• Journal of Korean Society of Water and Wastewater
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• v.32 no.5
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• pp.389-398
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• 2018

A nationwide survey of 8 N-nitrosamines in finished water samples from drinking water treatment plants (DWTPs) in Korea was conducted. The samples were pre-treated by solid-phase extraction (SPE) and analyzed using a gas chromatography coupled with tandem mass spectrometry (GC-MS/MS). According to the study results, four N-nitrosamines (NDMA, NDEA, NMOR, NDBA) were detected for three consecutive years, NMEA and NPYR were only found in samples collected in 2013. Two of these N-nitrosamines, N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA), have received attention and were the most commonly detected. The concentration of NDMA and NDEA in this study ranged from $0.002{\mu}g/L$ to $0.013{\mu}g/L$ and in $0.001{\mu}g/L$ to $0.008{\mu}g/L$, respectively. In comparison to studies performed in EPA(UCMR2), the concentrations of NDMA (from $0.002{\mu}g/L$ to $0.630{\mu}g/L$) and NDEA (from $0.005{\mu}g/L$ to $0.100{\mu}g/L$) observed in the this study were low.

• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1961-1967
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• 2006

Levels of ethyl carbamate, a potential carcinogen produced naturally during fermentation, in major Korean fermented foods and alcoholic beverages were determined by GC/MS/SIM, and their average daily intake and excess cancer risk in Korean people were estimated. In GC/MS/SIM analysis n.d.-4.26, 1.40-58.90, n.d.-3.76, n.d.-1.87, and 0.40-10.07 $\mu$g/kg of ethyl carbamate were detected in kimchi, soy sauces, fermented pastes, fermented dairy products, and alcoholic beverages, respectively. The average daily intake of ethyl carbamate and excess cancer risk through major Korean fermented foods and alcoholic beverage consumption were 6.0 ng/kg bw/day and $3.0\times10^{-7}$, respectively for the average Korean person aged 3-64 years, and were mainly contributed by Chinese cabbage kimchi, soy sauces, and Soju.

• Applied Biological Chemistry
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• v.36 no.2
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• pp.134-137
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• 1993

Yongia sonchifolia Max. has been used as raw materials of traditional Kimchi and medicinal herb in Korea. This study was performed to investigate biologically active components in the plant. First, the writer carried out the experiment of antitumor screening test against Sarcoma-180A and the cytotoxic activity against Chinese hamster V-79 cells with methanol extract of the plant. And the aqueous solution of the extract from roots of Youngia sonchifolia Max. was partitioned into n-hexane. The concentrated extract of n-hexane layer was chromatographed on silica gel column and developed with n-hexane and ethylacetate. Two yellow elutes, on concentration, were recrystallized from ethylacetate, and the $R_f$ value of TLC of the crystal was 0.43. After analysis by $^{1}H-NMR$, $^{13}C-NMR$ and MS to confirm the structure, the author could identify the compound as bauerenyl acetate, a naturally occurring pentacyclic triterpene. The crystal was colorless plate and m.p. was $280{\sim}282^{\circ}C$.

• Proceedings of the Korean Society for Cognitive Science Conference
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• 2000.05a
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• pp.55-60
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• 2000

연속재인과제에서 자극을 즉각적으로 반복 제시하거나 지연 반복 제시할 경우 사건관련전위에 차이가 나타나는지, 만약 차이가 관찰되면 자극 제시 후 어느 시점에서 차이가 두드러지게 나타나는지를 조사하였다. 실험 결과 즉각적 재인이 지연 재인에 비해 더 높은 반응정확율과 더 빠른 반응시간을 보였고 사건관련전위에서는 P300의 진폭이 더 컸고 잠재시간도 더 빨랐다. 그리고 지연재인에서 관찰되는 N400이 즉각적 재인에서는 나타나지 않았다. 지연재인에 비해 즉각적 재인에서 P300의 진폭이 더 크고 잠재시간이 더 빠르게 나타난 것은 P300이 template matching을 통한 정보처리 과정을 반영해 준다는 주장을 지지해 준다. 즉 즉각적 재인에서는 지연 재인에 비해 template matching이 훨씬 더 정확하고 빨리 일어나며 이 결과 P300의 진폭이 커지고 잠재시간이 더 빨라진 것으로 이해된다. 즉각적 재인에서는 방금 제시된 자극(시간 간격은 2초)을 재인하기 때문에 기억의 탐색 과정이 요구되지 않는 반면 5개의 간섭 단어 후에 반복 제시되는 단어를 재인하는 지연 재인에서는 단어에 대한 탐색 과정이 필요하며, 이에 따라 N400이 초래된 것으로 여겨진다. 이 결과는 N400이 기억 탐색 과정을 반영한다는 주장을 지지해 준다. 사건관련 전위에서 즉각적 재인과 지연 재인의 차이는 자극 제시 300ms에서 450ms사이에 가장 두드러지게 나타났다.

• Environmental Analysis Health and Toxicology
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• v.23 no.2
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• pp.113-117
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• 2008

Biomonitoring of nonylphenol (NP), an endocrine disrupting chemical, is required in Korea to perform its proper regulation. Thus, we analyzed exposure levels of nonlyphenol (NP) in breast milk from the mothers who delivered babies within 10 days (N=325). We analyzed free and total forms of NP in breast milk with LC/MS/MS (LOD, 0.5 ppb). In addition, we obtained questionnaires concerning lifestyle from the subject. As results, ranges of total NP were ${\mu}g/L$ (median, $3.51{\pm}4.98{\mu}g/L$ vs. normal, N=281, $2.07{\pm}3.76{\mu}g/L$; p<0.05). In conclusion, we suggest that exposure monitoring of NP should be continuously performed, even though the risks of NP are not clear, yet.

• Mycobiology
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• v.41 no.3
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• pp.149-154
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• 2013

An ${\alpha}$-glucosidase inhibitor was developed from Aspergillus oryzae N159-1, which was screened from traditional fermented Korean foods. The intracellular concentration of the inhibitor reached its highest level when the fungus was cultured in tryptic soy broth medium at $27^{\circ}C$ for five days. The inhibitor was purified using a series of purification steps involving ultrafiltration, Sephadex G-25 gel permeation chromatography, strong cation exchange solid phase extraction, reverse-phase high performance liquid chromatography, and size exclusion chromatography. The final yield of the purification was 1.9%. Results of the liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis indicated that the purified ${\alpha}$-glucosidase inhibitor was a tri-peptide, Pro-Phe-Pro, with the molecular weight of 360.1 Da. The IC50 value of the peptide against ${\alpha}$-glucosidase activity was 3.1 mg/mL. Using Lineweaver-Burk plot analysis, the inhibition pattern indicated that the inhibitor acts as a mixed type inhibitor.

• Macromolecular Research
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• v.14 no.5
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• pp.491-498
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• 2006

The thermal degradation of poly(hexamethylene guanidine) phosphate (PHMG) was studied by dynamic thermogravimetric analysis (TGA) and pyrolysis-GC/MS (p-GC). Thermal degradation of PHMG occurs in three different processes, such as dephosphorylation, sublimation/vaporization of amine compounds and decomposition/ recombination of hydrocarbon residues. The kinetic parameters of each stage were calculated from the Kissinger, Friedman and Flynn-Wall-Ozawa methods. The Chang method was also used for comparison study. To investigate the degradation mechanisms of the three different stages, the Coats-Redfern and the Phadnis-Deshpande methods were employed. The probable degradation mechanism for the first stage was a nucleation and growth mechanism, $A_n$ type. However, a power law and a diffusion mechanism, $D_n$ type, were operated for the second degradation stage, whereas a nucleation and growth mechanism, $A_n$ type, were operated again for the third degradation stage of PHMG. The theoretical weight loss against temperature curves, calculated by the estimated kinetic parameters, well fit the experimental data, thereby confirming the validity of the analysis method used in this work. The life-time predicted from the kinetic equation is a valuable guide for the thermal processing of PHMG.

• Molecules and Cells
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• v.27 no.1
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• pp.83-88
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• 2009

Amino acid homology analysis predicted that rbmD, a putative glycosyltransferase from Streptomyces ribosidificus ATCC 21294, has the highest homology with neoD in neomycin biosynthesis. S. fradiae BS1, in which the production of neomycin was abolished, was generated by disruption of the neoD gene in the neomycin producer S. fradiae. The restoration of neomycin by self complementation suggested that there was no polar effect in the mutant. In addition, S. fradiae BS6 was created with complementation by rbmD in S. fradiae BS1, and secondary metabolite analysis by ESI/MS, LC/MS and MS/MS showed the restoration of neomycin production in S. fradiae BS6. These gene inactivation and complementation studies suggested that, like neoD, rbmD functions as a 2-N-acetlyglucosaminyltransferase and demonstrated the potential for the generation of novel aminoglycoside antibiotics using glycosyltransferases in vivo.

• International Journal of Concrete Structures and Materials
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• v.9 no.3
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• pp.323-335
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• 2015

This paper examines some properties of concrete, such as strength, oxygen permeability and sorptivity using starch [cassava (CA) and maize (MS)] as admixtures. Concrete cubes containing different percentages of the CA and MS by weight of cement (0, 0.5, 1.0, 1.5 and 2.0 %) were cast. Compressive strength tests were carried out after 3, 7, 14, 21, 28, 56, 90, 180, 270 and 365 days of curing. Oxygen permeability and sorptivity tests were carried out on another set of concrete specimens with the same percentages of starch at 7, 28, 90, 180, 270 and 365 days. Oxygen permeability and sorptivity tests data obtained were subjected to Kruskal-Wallis one-way analysis of variance by ranks. The strength increase after 1 year over the control for CA 0.5 and CA 1.0 are 2.7 and 3.8 % respectively, while MS 0.5 and MS 1.0 gave 1.5 % increase over control. These results showed a decrease in oxygen permeability and rates of sorptivity, with concretes containing starch as admixtures giving better performance than the control concretes.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 2002.05a
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• pp.44-50
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• 2002

The fast Fourier transform (FFT) is a good method to estimate spectral density, but the frequency resolution is limited to the sampling window, and thus the precise characteristics of the spectral density for short signals are not clear. To solve the limitation, a multiple band-pass filter was introduced to estimate the precise time course of the spectral density for flash visual evoked potentials (VEPs). Signals were recorded during -200 and 600 ms using balanced noncephalic electrodes, and sampled at 1 K Hz in 12 bits. With 1 Hz and 10 ms resolutions, spectral density was estimated between 10 and 100 Hz. Background powers at the alpha-and beta-bands were high over the posterior scalp, and powers around 200ms were evoked at the same bands over the same region, corresponding to P110 and N165 of VEPs. normalized's spectral density showed evoked powers around 200 ms and suppressed powers following the evoked powers over the posterior scalp. The evoked powers above the 20Hz band were not statistically significant. However, the gamma band was significantly evoked intra-individually; details in the gamma bands were varied among the subjects. Details of spectral density were complicated even for a simple task such as watching flashes; both synchronization and desynchronization occurred with different distributions and different time courses.