• Title/Summary/Keyword: $M/H_2/1$ model

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Phosphoprotein Partitioning in Metal-Affinity Aqueous Two-Phase Systems and Prediction of Partitioning Behavior (금속 친화성 액 이상분계 시스템에서 Phsphoprotein분배 및 분배예측)

  • 정봉현
    • KSBB Journal
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    • v.9 no.3
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    • pp.279-286
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    • 1994
  • A mathematical model has been derived and used to describe phosphoprotein partitioning in Fe(III) IDA-PEG/dextran two-phase systems. This model includes the inhibitory effects of hydrogen and hydroxyl ion concentrations on protein partitioning. For aqueous two-phase partitioning experiments, the Al and A2 subcomponents of ovalbumin carrying two and one surface phosphoryl group(s) were purified using an immobilized metal ion affinity chromatography (IMAC). The ratio of partition coefficients in the presence and absence of Fe(III)IDA-PEG, K/Ko, increased in the pH range of 3.0 to 5.0 due to deprotonation of the second oxygen of the phosphoryl group, and above pH 5.0 declined steeply by the inhibitory binding of hydroxyl ions to the metal ion. This partitioning behavior was well described by the mathematical model. The binding constants for formation of the complex between the phosphoryl group and the Fe(III)IDA-PEG were found to be $6.1{\times}10^3M^{-1} and 2.3{\times}10^4M^{-1}$ in the top and bottom phases, respectively. These values are 3-5 times those for interaction of Cu(II)IDA-PEG with a single surface-accessible histidine.

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Experimental study on the Behavior CFT Column to H-Beam Endplate Connections with Penetrated High Strength Bolts (II) (관통형 고력볼트를 사용한 엔드플레이트형식 콘크리트 충전 각형강관 기둥.H형강 보 접합부의 거동에 관한 실험적 연구 (II))

  • Kim, Jae Keon;Moon, Tae Sup
    • Journal of Korean Society of Steel Construction
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    • v.11 no.2 s.39
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    • pp.109-116
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    • 1999
  • This paper presents an experimental study on the behavior of CFT Column to H-Beam Endplate Connections with penetrated H/T bolts under monotonic loading. The object of this study is to estimate accurately the effect about the thickness of endplate and the arrangement of H/T bolts which was not got a grip on the results reported in the previous paper. Main parameters are the thickness of endplates (12mm, 16mm) and the arrangemement of H/T bolts (EP1, EP2, EP3 Type). The experimental results compared and analysed. 1) The specimens were classified by Bjorhovde's and EC3's method. 2) A formula to predict the ultimate moment of connection was derived based on the T-stub model, and theoretical value $(_tM_u)$ computed by the formula corresponded to the experimental value $(_eM_u)$.

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Fabrication and pH response characteristics of LAPS(Light addressable potentiometric sensor) with electrolyte/$Si_3N_4/SiO_2$/Si structure (Electrolyte/$Si_3N_4/SiO_2/Si$ 구조의 LAPS 제작 및 pH 응답특성)

  • Chang Su-Won;Koh Kwang-Nak;Kang Shin-Won
    • Journal of the Korean Electrochemical Society
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    • v.1 no.1
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    • pp.40-44
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    • 1998
  • The LAPS device of fast response and high sensitivity, based on electrochemical potential difference, and its system were fabricated for the precise measurement of pH changes and its characteristic were investigated. The electrostatic variation characteristics of LAPS according to the pH changes and parameters in the device were verified through a simulation using LAPS equivalent circuit model. The LAPS device and its system were fabricated on the basis of the result of simulation. The fabricated LAPS system showed linear sensitivity (about 56 mV/pH within the range of pH 2 to pH 11. In order to overcome the defect of general urea sensor (especially slow response time), urease immobilized nitrocellulose membrane was attached on the LAPS and resulted in the very fast response time, 0.29 mV/sec, 0.86 mV/sec at urea concentration of $50{\mu}g/ml,\; 500{\mu}g/ml$, respectively. And also in order to measure the uranyl ion, the uranyl ion selective sensing membrane with calix[6]arene derivative was used and its sensitivity was 25mV/concentration decade in the wide uranyl ion concentration range of $10^{-11}M\;to\;10^{-4}M$.

A Study on the Development of Hydroelastic Experimental Techniques of Very Large Box-shaped Floating Structures with Shallow Draft (천흘수 부유식 해양 구조물의 유탄성 모형시험 기법 개발에 관한 연구)

  • H. Shin;I.K. Park;H.S. Shin;S.K. Kim;Y.S. Yang
    • Journal of the Society of Naval Architects of Korea
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    • v.36 no.4
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    • pp.64-76
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    • 1999
  • In this paper hydroelastic experimental techniques of very large floating offshore structures are suggested based on the model test carried out in the UOU Ocean Engineering Wide Tank. The prototype is a box-shaped floating structure with length of 300m, breadth of 60m, depth of 2m and draft of 0.5m and longitudinal bending rigidity as $4.87{\times}10^{10}kgm^2$. The scale ratio is 1/42.857. The model is realized by aluminum square pipes with the section dimension of $20mm{\times}20mm$. The numbers of longitudinal and transverse pipes are 7 and 35 respectively. Heave motions at selected points are measured with potentiometers and bending moments with strain gages.

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Derivation of Biochemical and Biophysical Parameters and Their Application to the Simple Biosphere Model (SiB2) (생화학 및 생물리 모수들의 도출과 생권 모형(SiB2)에의 적용)

  • Chae Nam-Yi;Kim Joon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.1 no.1
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    • pp.52-59
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    • 1999
  • Vegetation canopy plays an important role in $CO_2$/$H_2$O exchange between the biosphere and the atmosphere by controlling leaf stomata. In this study, rice (Oryza sativa L.), a staple crop in Asia was investigated to formulate its single leaf model of photosynthesis and stomatal conductance. Photosynthesis and stomatal conductance were measured with a portable infrared gas analyzer system. Other plant and meteorological variables were also measured. To evaluate empirical constants in this biochemical leaf model, nonlinear least squares technique was used. The maximum catalytic activity of enzyme and the maximum rate of electron transport were $ 100\mu$$m^{-2}$ $s^{-1}$ and $140 \mu$㏖ m$^{-2}$ s$^{-1}$ (@ 35$^{\circ}C$), respectively. The empirical constants, m and b, associated with stomatal conductance model were 9.7 and $0.06 m^{-2}$ $s^{-1}$ , respectively. On a leaf scale, agreements between the modeled and the measured values of photosynthesis and stomatal conductance were on average within 20%, and the simulation of diurnal variation was also satisfactory On a canopy scale, the Simple Biosphere model(SiB2) was tested using the derived parameters. The modeled energy fluxes were compared against the micrometeorologically measured fluxes over a rice canopy. Agreements between the modeled and the measured values of net radiation, sensible heat and latent heat fluxes, and $CO_2$ flux (i.e., net canopy photosynthesis) were on average within 25%.

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Effect of Phenolic Acids on Inhibition of Browning of Maillard Reaction Model Solutions (Phenolic acid가 Maillard 반응 모델액의 갈변억제에 미치는 영향)

  • Kwak, Eun-Jung;Lim, Seong-Il
    • Korean Journal of Food Science and Technology
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    • v.39 no.1
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    • pp.20-24
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    • 2007
  • The effects of phenolic acids on inhibition of browning by the Maillard reaction were investigated with a glucose-glutamic acid model for doenjang with citric acid as the antibrowning agent and phenolic acid as its synergist. Five phenolic acids, cinnamic, coumaric, caffeic, hydroxybenzoic, and protocatechuic acids, were used. In order to investigate the antibrowning capacity, 0.1M glucose, 0.1M glutamic acid, 50mM citric acid, and 1mM phenolic acid were dissolved in 1M phosphate buffer (pH 7.0), heated at $50^{\circ}C$ for 24hr in the presence of 0.2mM $FeCl_{2}$, and stored at $4^{\circ}C$ or $30^{\circ}C$ for four weeks. Phenolic acid addition more efficiently inhibited browning during storage at $30^{\circ}C$ than at $4^{\circ}C$, without changes in pH. Hydroxybenzoic acid was the most efficient and increased the antibrowning capacity by 13% compared to sample without phenolic acids. Although caffeic and protocatechuic acids inhibited most the formation of 3-deoxyglucosone or fluorescence, they increased browning by forming colored complexes between two hydroxy groups of phenolic acids and iron ions. Hydroxybenzoic acid will be able to be a useful synergist of citric acid, an antibrowning agent in doenjang, since it is permitted for doenjang.

Development of Kinetic Models Describing Kinetic Behavior of Bacillus cereus and Staphylococcus aureus in Milk

  • Kim, Hyoun Wook;Lee, Sun-Ah;Yoon, Yohan;Paik, Hyun-Dong;Ham, Jun-Sang;Han, Sang-Ha;Seo, Kuk-Hwan;Jang, Aera;Park, Bum-Young;Oh, Mi-Hwa
    • Food Science of Animal Resources
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    • v.33 no.2
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    • pp.155-161
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    • 2013
  • This study developed predictive models to evaluate the kinetic behaviors of Bacillus cereus and Staphylococcus aureus in milk during storage at various temperatures. B. cereus and S. aureus (3 Log CFU/mL) were inoculated into milk and stored at $10^{\circ}C$, $15^{\circ}C$, $20^{\circ}C$, and $30^{\circ}C$, as well as $5^{\circ}C$, $15^{\circ}C$, $25^{\circ}C$, and $35^{\circ}C$, respectively, while bacterial populations were enumerated. The growth data were fitted to the modified Gompertz model to estimate kinetic parameters, including the maximum specific growth rate (${\mu}_{max}$; Log CFU/[$mL{\cdot}h$]), lag phase duration (LPD; h), lower asymptote ($N_0$; Log CFU/mL), and upper asymptote ($N_{max}$; Log CFU/mL). To describe the kinetic behavior of B. cereus and S. aureus, the parameters were fitted to the square root model as a function of storage temperature. Finally, the developed models were validated with the observed data, and Bias (B) and Accuracy (A) factors were calculated. Cell counts of both bacteria increased with storage time. Primary modeling yielded the following parameters; ${\mu}_{max}$: 0.14-0.75 and 0.06-0.51 Log CFU/mL/h; LPD: 1.78-14.03 and 0.00-1.44 h, $N_0$: 3.10-3.37 and 2.09-3.07 Log CFU/mL, and $N_{max}$: 7.59-8.87 and 8.60-9.32 Log CFU/mL for B. cereus and S. aureus, respectively. Secondary modeling yielded a determination of coefficient ($R^2$) of 0.926.0.996. B factors were 1.20 and 0.94, and A factors were 1.16 and 1.08 for B. cereus and S. aureus, respectively. Thus, the mathematical models developed here should be useful in describing the kinetic behaviors of B. cereus and S. aureus in milk during storage.

DISCOVERY OF A STRONG LENSING GALAXY EMBEDDED IN A CLUSTER AT z = 1.62

  • WONG, KENNETH C.;TRAN, KIM-VY H.;SUYU, SHERRY H.;MOMCHEVA, IVELINA G.;BRAMMER, GABRIEL B.;BRODWIN, MARK;GONZALEZ, ANTHONY H.;HALKOLA, ALEKSI;KACPRZAK, GLENN G.;KOEKEMOER, ANTON M.;PAPOVICH, CASEY J.;RUDNICK, GREGORY H.
    • Publications of The Korean Astronomical Society
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    • v.30 no.2
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    • pp.389-392
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    • 2015
  • We identify a strong lensing galaxy in the cluster IRC 0218 that is spectroscopically confirmed to be at z = 1.62, making it the highest-redshift strong lens galaxy known. The lens is one of the two brightest cluster galaxies and lenses a background source galaxy into an arc and a counterimage. With Hubble Space Telescope (HST) grism and Keck/LRIS spectroscopy, we measure the source redshift to be $z_S=2.26$. Using HST imaging, we model the lens mass distribution with an elliptical power-law profile and account for the effects of the cluster halo and nearby galaxies. The Einstein radius is $^{\theta}E=0.38^{+0.02{\prime}{\prime}}_{-0.01}$ ($3.2^{+0.2}_{-0.1}kpc$) and the total enclosed mass is $M_{tot}(<^{\theta}_E)=1.8^{+0.2}_{-0.1}{\times}10^{11}M_{\odot}$. We estimate that the cluster environment contributes ~ 10% of this total mass. Assuming a Chabrier IMF, the dark matter fraction within $^{\theta}E$ is $f^{Chab}_{DM}=0.3^{+0.1}_{-0.3}$, while a Salpeter IMF is marginally inconsistent with the enclosed mass ($f^{Salp}_{DM}=-0.3^{+0.2}_{-0.5}$).

Characteristics of Wave Propagation by Water Level Conditions at Wando Sea Area: Numerical Modeling (완도 해역의 해수면 조건에 따른 파랑 변형 특성)

  • Jeon, Yong-Ho;Yoon, Han-Sam;Kim, Dong-Hwan;Kim, Won-Seok;Kim, Heon-Tae
    • Journal of Fisheries and Marine Sciences Education
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    • v.25 no.1
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    • pp.1-11
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    • 2013
  • The aim of this study was estimated the characteristics of the wave propagation by the water level conditions using a numerical modeling method at the Wando sea area. For three cases numerical simulation on the condition of incident and incoming of the deepwater design wave and the season normal wave, the spatial distribution of the incident wave at study area were investigated. And the calculated numerical modeling results were compared with measured field wave data. According to on-site wave data measured for 18 days, the range of the significant wave height and period were 0.10~1.14 m, 4.35~8.74 sec, respectively, and the maximum wave height were 0.15~1.66 m. From the results of numerical model for offshore design wave incident, the wave height attacked from Southern-East direction at this study area were over maximum 10.5 m because of rapidly change of water depth. Numerical modeling by three water level conditions of Approxmate Lowest Low Water Level(Approx. L.L.W), Mean Sea Level(M.S.L) and Approximate Highest High Water Level(Approx. H.H.W) were practiced. From the results for the case of Approx. H.W.L, variations of wave height at the back area of islands were about 1.6 m at maximum value for the case of deepwater design wave incoming. The significant wave heights of winter season were bigger than summer under normal wave condition, the incident wave height over 5.5 m decreased by shielding effect of islands. The change of maximum wave height at summer season were distinct than winter and was about 1.2 m and 0.8 m, respectively.

A Model Experiment on the Basic Efficiency of Midwater Rope Trawl Net (로프 트롤 그물의 기본성능에 관한 모형실험)

  • Yae, Young-Hee;Lee, Byong-Gee
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.29 no.3
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    • pp.200-213
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    • 1993
  • A model experiment on a midwater rope trawl net which is used in the North Pacific to catch alaska pollack is carried out in the circulating tank to examine the basic efficiency of the net. The prototype is the net used by M/S Hanil(1, 179GT, 2, 700PS), a Korean trawler. The model net was made according to the Tauti's Similarity Law of Fishing Gear in 1/100 scale by considering the condition of the tank. To measure the basic efficiency of the standard model net, the vertical opening and width between some points marked on the net were measured, and the hydrodynamic resistance were determined. Then the constructive conditions of the net were varied as follows and the factors were measured again to compare the efficiency of those nets with that of the standard net(A-1 type) front weight multiplied 1.5 times: A-2 type. buoyancy and depressing force multiplied 1.7 times: A-3 type. front weight multiplied 1.5 times on A-3 type: A-4 type. depressors rigged at ground rope: B type. cod-end stuffed with cashmylon wad: C type. The results obtained can be summarized as follows: 1. The vertical opening at the center of head rope was steeply decreased with the flow velocity increasing and the vertical opening H(m) can be expressed in H=1.2v super(-1.2)(v : flow velocity in m/sec). The width of the net varied a little when the flow velocity was over 0.4m/sec, and the width of net mouth showed about 37% of the distance between the fore tips of net pendant. The shape of net mouth was almost a circle at 0.2m/sec and then steeply flatted elliptically with the flow velocity increasing and the area of mouth S(m super(2)) can be expressed in S=(1.65-2.3v)$\times$10 super(-2). The hydrodynamic resistance of the net increased almost linearly with the flow velocity increasing and the resistance R(kg) can be expressed in R=3.2$\times$d/l$\times$abv. where d/l denotes the mean of d(diameter of netting twine) and l(length of a leg in a mesh) from wing tip to the end of bag-net except cod-end on the side pannel, and a denotes the strectched circumference of the net at the fore end of a meshed part and b the stretched length of the whole net from wing tip to the end of cod-end. 2. In the condition-varied nets, the vertical opening of head rope showed some increase in every type net except the C type, and the increase showed the greatest in the B type by 30~54%, whereas it showed decrease in the C type by 5~10%. Variation of the area of net mouth showed almost the same tendency as the vertical opening and the increase showed the greatest in the B type by 20%, whereas it showed decrease in the C type by 12%. Hydrodynamic resistance showed some increase in every type compared with the standard net, and the rate of increase indicated 5~10% in the A-2, A-3 and A-4 type, 22% in the B type and 3% in the C type.

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