• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 공동 춘계학술대회
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• pp.97.1-97
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• 2001

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• Food Quality and Culture
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• 제1권1호
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• pp.18-21
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• 2007

The objective of this study was to prepare bioactive ginseng yogurts containing compound K, which is transformed from ginsenosides, and to investigate the compound's cytotoxicity against tumor cells. Milk containing ginseng was fermented by Bifidobacteria KK-I and KK-2, and their activities for transforming ginsenosides to compound K were measured. Among the tested concentrations of ginseng in the milk, compound K was effectively produced in the 3% and 6% ginseng yogurts fermented for 48 hrs. These fermented ginseng yogurts were extracted with BuOH, and their cytotoxicities against tumor cells were examined. The BuOH extract of the yogurt made from the 3% ginseng milk showed cytotoxic activity against P388 and HeLa tumor cells. However, the nonfermented ginseng milk did not exhibit cytotoxicity against these cells. Therefore, we deem that the ginseng yogurt, which contained compound K, could be developed as a potential fermented drink product.

• KSBB Journal
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• 제29권6호
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• pp.443-447
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• 2014

Biosynthesis of lactate-containing polyhydroxyalkanoates (PHAs) was examined in recombinant Escherichia coli W strain from sucrose. The Pseudomonas sp. MBEL 6-19 phaC1437 gene and the Clostridium propionicum pct540 gene, which encode engineered Pseudomonas sp. MBEL 6-19 PHA synthase 1 ($PhaC1_{Ps6-19}$) and engineered C. propionicum propionyl-CoA transferase ($Pct_{Cp}$), respectively, were expressed in E. coli W to construct key metabolic pathway to produce poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)]. The recombinant E. coli W expressing the phaC1437 gene and the pct540 gene could synthesize P(3HB-co-13mol%LA) up to the polymer content of 31.3 wt% when it was cultured in chemically defined MR medium containing 20 g/L of sucrose and 2 g/L of sodium 3-hydroxybutyrate. When Ralstonia eutropha phaAB genes were additionally expressed to provide 3-hydroxybutyrate-CoA (3HB-CoA) from sucrose, P(3HB-co-16mol%LA) could be synthesized from sucrose as a sole carbon source without supplement of sodium 3-hydroxybutyrate in culture medium, but the PHA content was decreased to 12.2 wt%. The molecular weight of P(3HB-co-16 mol%LA) synthesized in E. coli W using sucrose as carbon source were $1.53{\times}10^4$ ($M_n$) and $2.78{\times}10^4$ ($M_w$), respectively, which are not different from those that have previously been reported by other recombinant E. coli strains. Engineered E. coli strains developed in this study should be useful for the production of lactate-containing PHAs from sucrose, one of the most abundant and least expensive carbon sources.

• 농촌계획
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• 제21권3호
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• pp.47-58
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• 2015

This study aimed to explore how to lead locals to join rural downtown regeneration projects on the basis of the downtown of Obuse Town located in Nagano Prefecture in Japan. What well depicts the outcome of Obuse Town Project joined by residents are revitalized downtown districts and open gardens. Actors that facilitated residents' participation are administration, "a-la Obuse Co. Ltd. and "Machizukuri Institude of TUS and Obuse Town". "a-la- Obuse Co. Ltd." is a middle support organization in the form of community business and "Machizukuri Institute of TUS and Obuse Town" is a professional research institute set by Tokyo University of Science and Obuse Town. This study presented activities, types and roles of residents' participation in the rural town activation project that started in 2015 by researching the activities of residents, shop owners and students with regard to Obuse Town Project, administrative actions for their participation and activities of middle-level supportive institutions, which are exports' research groups.

• Radiation Oncology Journal
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• 제23권1호
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• pp.51-60
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• 2005

목적 : 분자 표적의 선택적 억제가 방사선 세포 살상 효과를 증진시키는 것으로 알려져 있으므로 선택적 COX-2 억제제와 EGF 수용체 차단제를 HeLa 세포주에 처리한 후 방사선 효과의 상승작용을 알아보고자 하였다. 대상 및 방법 : 자궁경부암 세포주인 HeLa세포에서 EGF 수용체 차단제 AG 1478, 선택적 COX-2 억제제 NS 398과 방사선을 복합 투여하여 세포성장 억제 분석(cell graph inhibition assay)과 세포사멸 분석(apoptosis assay)을 시행하였고, 방사선 감수성 변화를 살펴보기 위해 세포생존 분석(clonogenic survival assay)을 시행하였다. 방사선 감수성 인자로는 2 Gy에서의 세포생존분획($SF_2$)과 linear-quadratic model을 이용한 dose enhancement ratio (DER)를 사용하였다. 방사선 감수성에 대한 작용기전 분석을 위해 flow cytometry로 세포주기 분석(cell cycle analysls)을 시행하였고, western blot 분석을 통하여 bcl-2와 bax 단백질의 발현 변화를 살펴보았다. 결과 : HeLa세포에 NS 398과 AG 1478을 방사선과 함께 복합 투여한 실험 군에서 세포사멸 정도가 가장 높게 나타났다($8.49\%$ vs. $22.70\%$). 세포주기 분석 결과, 방사선과 복합 약물 처리군에서 $G_0/G_l$ 세포주기 정체와 5 세포 분획 소실이 나타났으며 이러한 변화는 72시간 이후까지 지속되었다 세포생존 분석 결과로는 방사선과 AG 1478군에서 $SF_{2}0.68{\pm}0.07$, DER 1.12를 보인 반면, 방사선과 복합약물처리군에서는 $SF_{2}0.12{\pm}0.01,\;DER\;3.00$으로 나타났다. Western blot분석에서는 방사선과 복합약물처리군에서 bcl-2와 bax 단백질 발현이 모두 감소하는 양상을 보였다. 결론 : 신호전달 체계를 억제하는 분자 표적 약제인 선택적 COX-2 억제제와 EGF 수용체 차단제를 방사선과 복합투여함으로써 HeLa세포의 방사선 감수성이 증가됨을 확인하였다.

• 한국표면공학회지
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• 제50권6호
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• pp.465-472
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• 2017

The ingot fabrication conditions related with the thermal shock bearing phase and microstructure have investigated for the rare earth zirconate ceramic material, lanthanum gadolinium zirconate, as a thermal barrier coating using electron beam evaporation method. The thermal shock resistance of the prepared ingot was evaluated by high energy electron beam irradiation. The rare earth zirconate ceramic powder was prepared by controlling the raw material powder composition of $La_2O_3$, $Gd_2O_3$ and $ZrO_2$ so as to have a composition of $(La_{0.3}Gd_{0.7})_2Zr_2O_7$ which was selected from the former study. Ingot samples were prepared under two conditions. The first condition is prepared by sintering the prepared powder mixture to form an ingot. The second condition is prepared by calcining the prepared powder mixture to form a composite phase and then sintering to form an ingot. X-ray diffraction(XRD) and Scanning Electron Microscope(SEM) were used to analyze phase forming behavior and microstructure of ingot samples. Nanoindentation method used to obtain elastic modulus and hardness of each ingot specimen. Also the stress distribution of ingot was simulated by using FEM method assuming the ingot surface was exposed to electron beam. As a results, in the case of an ingot having a network-shaped microstructure in which relatively coarse pores are included, it seems that the thermal shock resistance was higher than in the case of an ingot having a microstructure composed of relatively fine grains only or particles with the similar level size when the high energy electron beam irradiation.

• 한국세라믹학회지
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• 제54권4호
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• pp.278-284
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• 2017

In this work, Ta-substituted $Li_7La_3Zr_{2-x}O_{12}$ (LLZTO) powder and pellets with garnet cubic structure were fabricated and characterized by modified and optimized sol-gel synthesis. Ta-substituted LLZO powder with the smallest grain size and pure cubic structure with little pyrochlore phase was obtained by synthesis method in which Li and La sources in propanol solvent were mixed together with Zr and Ta sources in 2-methoxy ethanol. The LLZTO pellets made with the prepared powder showed cubic garnet structure for all conditions when the amount of Li addition was varied from 6.2 to 7.4 mol. All the X-ray peaks of the pyrochlore phase disappeared when the Li addition was increased above 7.0 mol. When the final sintering temperature was varied, the LLZTO pellet had a pyrochlore-mixed cubic phase above $1000^{\circ}C$. However, the surface morphology became much denser when the final sintering temperature was increased. The sol-gel-driven LLZTO pellet with a sintering temperature of $1100^{\circ}C$ showed a lithium ionic conductivity of 0.21 mS/cm when Au was adopted as electrode material for the blocking capacitor. The results of this study suggest that modified sol-gel synthesis is the optimum method to obtain cubic phase of LLZTO powder for highly dense and conductive solid electrolyte ceramics.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2002년도 춘계학술대회 논문집 유기절연재료 전자세라믹 방전플라즈마 일렉트렛트 및 응용기술
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• pp.71-74
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• 2002

In this study, resonant characteristics of $Pb_{0.88}(La_{0.6}Nd_{0.4})_{0.08}(Mn_{1/3}Sb_{2/3})_{0.02}Ti_{0.98}O_3$ ceramics were investigated with the variations of electrode radius size for manufacturing the best 30MHz SMD type ceramic resonator with the size of $3.7{\times}3.1{\times}0.255mm^3$. Physical properties were proper for appling for 30MHz SMD type ceramic resonator. In third overtone thickness vibration mode, with increasing electrode radius size, resonant resistant($Z_r$) was decreased gradually. Mechanical quality factor($Q_{mt3}$) and dynamic range(D.R) showed the maximum value of 2,283, 47.1dB respectively, at 0.74mm electrode radius size.

• Fisheries and Aquatic Sciences
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• 제18권1호
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• pp.89-98
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• 2015

A synthesis method for a chitosan-coated magnetic drug-delivery system of cisplatin is proposed. Here, cisplatin was conjugated to the surface of Magnetite ($Fe_3O_4$) nanoparticles via a (3-Aminopropyl)-trimethoxysilane (APTS) coupling agent. To reduce the cytotoxic effect of cisplatin, the magnetic drug was then encapsulated in chitosan (CS-cisplatin-$Fe_3O_4$) through the water/oil (W/O) emulsion method. The CS-cisplatin-$Fe_3O_4$ nanoparticles were synthesized in a spherical shape with a diameter of 190 nm. The cytotoxicity assay was performed using HeLa cells. The cisplatin uptake of the cells was determined using High Performance Liquid Chromatography (HPLC) to calculate the drug content. The controlled release of cisplatin was demonstrated by regulating the dissolution and diffusion of the drug through the chitosan matrix.

• 한국식품영양과학회지
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• 제35권10호
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• pp.1291-1296
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• 2006

메밀 국수의 주 재료인 메밀분의 함량을 60%와 70%로 다르게 하여 건면, 압출면 그리고, 생면의 추출물을 조제하여 항돌연변이원성과 세포독성 효과를 조사하였다. 세포독성 효과를 알아보기 위해서 A549, Hep3B, MCF-7, AGS와 HeLa를 사용하였다. 이들 시료의 추출물의 암세포 성장 억제효과를 조사한 결과, 메밀 생면은 자궁암세포에서 68.5%의 높은 억제 활성을 나타내었으며 압출면의 경우는 위암세포에서 75.7%의 억제율을 나타내었다. 건면의 경우는 폐암 세포에서 80%의 높은 억제효과를 나타내었다. 그리고 돌연 변이성 및 항돌연변이 활성은 S. Typhimurium TA98과 TA100 균주를 이용한 돌연변이성 실험 결과 메밀면 자체에는 돌연변이성이 없었으며, 항돌연변이원성 실험에서는 직접변이원인 MNNG$(0.4{\mu}g/place)$의 경우 TA100 균주에서 시료농도 $160{\mu}g/place$에서 메밀분 70% 혼합분 생면 추출물의 경우 41%의 억제효과를 나타내었으며 4NQO$(0.15{\mu}g/place)$에 대해서는 같은 시료농도에서 TA98 균주에 대해서 43%의 억제 효과를 나타내었다. 그리고 메밀분 70% 혼합분 압출면의 경우 28%의 낮은 효과를 보였으나 S. Typhimurium TA98 균주에서는 같은 시료농도에서 45%의 억제효과를 나타내었으며 공시 시료 모두 농도 의존적으로 억제하는 것으로 나타났다.