• 제목/요약/키워드: $K_m$ value

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Association between a M-Iacking mutant D75N of pharaonis phoborhodopsin and its transducer is stronger than the complex of the wild-type pigment: Implication of the signal transduction

  • Sudo, Yuki;Iwamoto, Masayuki;Shimono, Kazumi;Kamo, Naoki
    • Journal of Photoscience
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    • 제9권2호
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    • pp.314-316
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    • 2002
  • In halobacterial membrane, pharaonis phoborhodopsin (or pharaonis sensory rhdopsin II, psRII) forms a complex with its transducer pHtrII. Flash-photolyis of D75N mutant did not yield M-intermediate but an O-like intermediate is observed. We examined the interaction between D75N of ppR and t-Htr (truncated pHtrII). These formed a complex in the presence of n-dodecyl-$\beta$-D-maltoside, and the association accelerated the decay of the 0 of D75N from 15 to 56 s$\^$-1/. From the decay time constants under varying ratios of D75N and t-Htr, n, the molar ratio of D75N/t-Htr in the complex, and K$\_$D/, the dissociation constant, were estimated. The value of n was unity and K$\_$D/ was estimated to 146 nM. This K$\_$D/ value can be considered as the association between the photo-intermediate and t-Htr, which is deduced by the method of estimation. Previously we (Photochem. Photobiol. 74, 489-494 (2001)) reported K$\_$D/ of 15 $\mu$M for the interaction between the wild-type and t-Htr by means of the change of M-decay rates. Therefore, this value should be the K$\_$D/ value for the interaction between M of the wild-type and t-Htr.

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내장산국립공원 굴거리나무군락의 군집특성 및 분포 변화 연구 (The Study of Distribution Changing and Community Characteristics of Daphniphyllum macropodum (National Monument No. 91) in Naejangsan National Park)

  • 신진호;전용삼;손지원
    • 한국환경복원기술학회지
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    • 제19권3호
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    • pp.45-57
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    • 2016
  • This study was focused on the northernmost limit of the distribution changing of Daphniphyllum macropodum and studied characteristic of plant communities of natural monument No. 91 in Najangsan national park, Korea. The results of this study were as follows : 1. The highest importance percentage(I.P.) value at tree layer in Quercus. mongolica community was Q. mongolica, 37.8%. But the highest value of mean importance percentage(M.I.P.) was D. macropodum, 32.8%. 2. The highest I.P. value at tree layer in Carpinus laxiflora community was C. laxiflora, 47.4%. The highest M.I.P. value was C. laxiflora, 28.8% and M.I.P. value of D. macropodum was 24.0%. 3. The highest I.P. value at tree layer in D. macropodum community was D. macropodum, 55.6%. C. laxiflora and Q. mogolica I.P. value was 14.8% and 6.8%, respectively. The highest M.I.P. value was D. macropodum, 47.9%. Sapium japonicum and C. laxiflora M.I.P. value was 11.4% and 10.7%, respectively. 4. The highest I.P. value at tree layer in Quercus variabilis community was C. laxiflora, 20.8%. Q. variabilis and Acer pseudosieboldianum I.P. value was 15.3% and 12.5%, respectively. The highest M.I.P. value was D. macropodum, 21.5%. It needs to the continuous monitoring of vegetation and importance percentage change in tree layer and subtree layer of D. macropodum. The researching results of D. macropodum distribution, the distribution range of D. macropodum showed expanded more than range of Lim and Oh' result(1999). In other words, it was distributed low density level between Najangsa temple and Byeokryeonam, and distributed in upper Keumsun waterfall and Wonjeok upper valley. D. macropodum was appeared on hiking trails around from Wonjeokam to Bulchulbong. Especially, it was found in this study that D. macropodum was distributed on Seoraebong's north which placed outside area of Naejangsa region. In this study, it was considered that distribution range of D. macropodum was expanded. Also, it is expected to be used as a result of the field study of changing distribution study of broad leaved evergreen forest due to global warming.

건식 쌀가루를 이용한 막편의 품질특성 (A Study on the Quality Characteristics of Makpyeon Prepared with Dry Milled Rice Powder)

  • 조윤주;윤혜현
    • 한국식생활문화학회지
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    • 제31권3호
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    • pp.235-242
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    • 2016
  • The purpose of this study was to investigate the quality characteristics of makpyeon prepared with dry milled rice powder. The makpyeon samples prepared with dry milled rice powder and various amount makgeolli (0% (CON), 25% (M25), 50% (M50), 75% (M75), 100% (M100)), and analyzed for moisture content, pH, Hunter's color value, TPA and sensory evaluation. The moisture content did not showed significant difference among makpyeon samples. The L-value, a-value resulted in that CON showed the highest and decreased with the amount of makgeolli. The b-value of makpyeon samples showed that CON was the lowest and increased with the amount of makgeolli. TPA resulted in that M100 showed the highest hardness and the lowest adhesiveness, cohesiveness. Chewiness and gumminess of makpyeon samples were higher than those of CON. Based on quantitative descriptive analysis, the score of brightness, moistness, particle size and gloss of showed higher in CON that in makpyeon samples, firmness increased with the amount of makgeolli. Flavor attributes of liquor odor, sour odor, fermentation odor of makpyeon was stronger in makpyeon samples that in CON with the amount of makgeolli. Acceptance test resulted in makpyeon made with CON, 100% of makgeolli (M100) showed the significantly highest score in taste and overall acceptance.

물푸레나무로부터 분리된 Esculetin와 Esculin의 미백 효능 (Depigmenting Effects of Esculetin and Esculin Isolated from Fraxinus rhynchophyllaHance)

  • 홍용덕;남미희;이창석;신송석;박영호
    • 대한화장품학회지
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    • 제40권1호
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    • pp.89-94
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    • 2014
  • 물푸레나무 수피 추출물은 주요 생활성 물질로서 esculetin과 esculin을 포함하고 있다. 이 가운데, Esculetin은 B16F10 melanoma cells에서 $IC_{50}$ value $2.8{\mu}M$의 아주 좋은 melanogenesis 억제 활성을 나타내며, 이를 통해 Melan-A cell에서 melanin 합성을 감소시킨다. 더욱이, esculetin은 mushroom tyrosinase에서도 $IC_{50}$ value $40{\mu}M$의 억제 활성을 나타내어 melanin biosynthesis를 저해한다. 위의 결과들로서, 우리는 melanogenic enzyme 활성 조절에 의해 melanin 생성을 저해하는 효과적인 피부미백 물질로서 esculetin을 제안하고자 한다.

Characteristics of a Low Molecular Weight Minor Anionic Isoperoxidase $A_{3n}$ from Radish

  • Lee, Mi-Young;Kim, Soung-Soo
    • BMB Reports
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    • 제31권6호
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    • pp.548-553
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    • 1998
  • A minor anionic isoperoxidase named $A_{3n}$, was isolated from Korean radish (Raphanus sativus L.) root. Purification of the enzyme was accomplished by CMcellulose chromatography, DEAE-Sephacel chromatography, and Sephadex G-75 gel filtration. The enzyme was a glycoprotein with molecular weight of approximately 31,000 as determined by SDS-PAGE and 33,000 by Sepadex G-150 gel filtration, which is by far the smallest among the reported isoperoxidases. The pI value was 3.5. The optimum pH of the enzyme was 6.5 for guaiacol and $H_2O_2$, and the $K_m$ values for guaiacol and $H_2O_2$ were 13.3 mM and 1.5 mM, respectively. Kinetic studies with various substrates revealed that only A3n, unlike other isoperoxidases from radish, did not use scopoletin as a substrate and had very low $K_m$ value of 0.25 mM for ferolic acid among naturally occurring phenolic substrates.

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Evaluation of crystallinity and defect on (100) ZnTe/GaAs grown by hot wall epitaxy

  • Kim, Beong-Ju
    • 한국결정성장학회지
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    • 제12권6호
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    • pp.299-303
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    • 2002
  • The relationship of crystallinity between defects distribution with (100) ZnTe/GaAs using HWE growth was investigated by four crystal rocking curve (FCRC) and transmission electron microscopy (TEM). The thickness dependence of crystal quality in ZnTe epilayer was evaluated. The FWHM value shows a strong dependence on ZnTe epilayer thickness. For the films thinner than 6 ${\mu}{\textrm}{m}$, the FWHM value decreases very steeply as the thickness increases. For the films thicker than 6 ${\mu}{\textrm}{m}$, it becomes an almost constant value. At the thickness of 12 $\mu\textrm{m}$ with the smallest value of 66 arcsec. which is the best value so far reported on ZnTe epilayers was obtained. Investigation into the nature and behavior of dislocations with film thickness in (100) ZnTe/(100)GaAs heterostructures grown by Hot Wall Epitaxy (HWE). This film defects range from interface to 0.7 ${\mu}{\textrm}{m}$ thickness was high density, due to the large lattice mismatch and thermal expansion coefficients. The thickness of 0.7~1.8 ${\mu}{\textrm}{m}$ was exists low defect density. In the thicker range than 1.8 ${\mu}{\textrm}{m}$ thickness was measured hardly defects.

Physiological Activities of Thiacremonone Produced in High Temperature and High Pressure Treated Garlic

  • Woo, Koan Sik;Hwang, In Guk;Kim, Hyun Young;Lee, Sang Hoon;Jeong, Heon Sang
    • Preventive Nutrition and Food Science
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    • 제21권1호
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    • pp.68-72
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    • 2016
  • To examine the possibility of using thiacremonone isolated from high-temperature-high-pressure treated garlic, this study investigated the physiological activities properties. The $IC_{50}$ values of hydroxyl, superoxide, hydrogen peroxide, and nitric oxide radical scavenging activities of thiacremonone were 92.50, 65.05, 12.60, and $81.53{\mu}g/mL$, respectively. On the other hand, the activities of vitamin C were 104.93, 99.43, 42.42, and $122.64{\mu}g/mL$, and the activities of butylated hydroxyanisole were 37.22, 68.45, 22.47, and $40.54{\mu}g/mL$, respectively. The $IC_{50}$ value of ACE inhibition activities of thiacremonone and captoprill were 0.265 and $0.036{\mu}g/mL$, respectively. The $IC_{50}$ value of xanthine oxidase inhibition activities of thiacremonone and allopurinol were 39.430 and $9.346{\mu}g/mL$, respectively. The $IC_{50}$ value of tyrosinase inhibition activities of thiacremonone and kojic acid were 101.931 and $65.648{\mu}g/mL$, respectively.

시판 폴리비닐실록산 인상재의 물성에 관한 비교연구 (Comparative study on properties of commercial polyvinyl siloxane impression materials)

  • 강재경
    • 한국치위생학회지
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    • 제1권2호
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    • pp.171-180
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    • 2001
  • The purpose of this study was to compared with the properties of commercial polyvinyl siloxane impression materials on the Korean product and the other country one. The materials used in this study were 5 hydrophilic polvinyly siloxane impression materials of light body automixing type (Perfect-F, Handae Chemical, Korea; Examix, GC, Japan; Contrast, VOCO, Germany; Express, 3M, U.S.A; Extrude, Kerr, U.S.A.). Specimens of each impression material were fabricated from a mold with dimensions identical to that specified in ASTM D624-91 and were subjected to tensile lode at 500 mm/min until failure for tear strength. Properties of consistency, strain in compression, recovery from deformation, detail reproduction and linear dimensional change were tested according to the testing methods of the ISO specification no.4823(1992). From this study, the follow ing results were obtained 1. The tear strength values were high ET(3.4kN/m), EP(3.4kN/m), PF(3.0kN/m), CT(3.0kN/m), but the lowest was EM(2.2kN/m)(p<0.05). 2. In the consistency, CT(47 mm) had the highest value, followed by PF(42.1 mm), ET(41.2 mm), and EM(39.6 mm), EP(39.2 mm)(p<0.05). It means more flow that the consistency value is high. 3. The strain in compression values were high followed by EM(5.8%), PF(52%), CT(4.6%), ET(4.1%), EP(2.9%)(p<0.05). 4. In the recovery from deformation, ET(99.95%) and PF(99.90%) had the highest value, followed by EP(99.75%), EM(99.74%) without statistical signification, CT(99.64%) had the lowest value(p<0.05). 5. Detail reproduction reappeared to line $20{\mu}m$ all products. 6. The linear dimensional change was a little shrinked all products. and there were not statistical signification (p>0.05). All products were satisfied the ISO specification.

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콘빔전산화단층촬영에서 노출 조건에 따른 화질 유지 및 선량 감소에 대한 평가 (Evaluation of Dose Reduction and Maintaining Image Quality according to Exposure Factors of Cone Beam Computed Tomography)

  • 한진우
    • 한국방사선학회논문지
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    • 제14권4호
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    • pp.353-360
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    • 2020
  • 본 연구는 CBCT 장비의 표준 노출 조건(80 kV, 7 mA)을 기준으로 관전압과 관전류를 낮추어 촬영하면서 표준 노출 조건의 물리적 화질 요소값을 유지할 수 있는 노출 조건의 제시를 목표로 두고자한다. 영상의 물리적 화질 요소값의 측정을 위해 변조전달함수(MTF)가 분석되었고 선량 측정을 위해 선량-면적 곱(DAP)을 이용하였다. 관전압(80, 78, 76 kV)과 관전류(7, 6, 5, 4, 3 mA)의 15가지 조합의 노출 조건에서 Sedent ex IQ 팬텀 (Leeds Test Objects Ltd., Boroughbridge, UK)의 CBCT 영상을 얻었고 MTF 10이 각 조건에서 계산 되었다. 표준 노출 조건과 비교시 80 kV-6 mA, 80 kV-5 mA 노출 조건은 MTF 10에 있어 유의한 차이를 보이지 않았기에 본 연구에서 사용된 CBCT 장비의 경우, 80 kV-5 mA로 낮춘 노출 조건에서 물리적 화질요소값을 유지하면서 선량을 감소시킬 수 있는 것으로 판단되어진다.