• 대한치과기공학회지
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• 제38권1호
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• pp.37-49
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• 2016

Purpose: This research is performed through the analysis of the dental technology students' life-style factors of their daily lives that have a close relation with health, and the self-reported symptoms which are related to psychosomatic diseases, for the production of basic data for the change of life-styles and the development of educational programmes. Methods: This research has been performed through questionnaires from the beginning of October of 2014 till the end of November through the Dental Technology students of G-do, chosen by random sampling method after informed consent, where out of the 270 papers, 258 results were used for the analysis. Results: Firstly, the results of the research was as follows: Non-smokers accounted for 61.2%, student who did not do regular physical activities accounted for 50.4%, students who had sleeping hours of 6 to 7 hours accounted for 35.7%, students who eat snacks accounted for 63.2%, students who eat breakfast once to twice a week accounted for 30.6%, students who had an average number of drinks of once to twice a week accounted for 39.9%, and students with BMI of '$18.5^{\circ}{{\neq}}22.9$' accounted for 56.2%, being the highest. Secondly, in gender, smoking (p<.001), regular physical activity (p<.001), BMI (p<.001), eat snacks (p<.05), average number of drinks (p<.05), and of the health check-up, eat breakfast (p<.05), of the year level, eat snack (p<.01), BMI (p<.01), hours of sleep (p<.05), of major satisfaction, regular physical activity (p<.01), and BMI (p<.05), and there was a significant statistical difference, but there were no significant statistical difference in religion. Thirdly, of the bodily self-reported symptoms of the correlation analysis within the life-style variables and the self-reported symptoms, multiple subjective symptoms (I), respiratory (A), eye and skin (B), and digestive organs (C) were the highest in the correlation analysis with regular physical activity (p<.01), and of mental health, impulsiveness (H), mental instability (J), and depression (K) showed highest results in correlation analysis with regular physical activity (p<.01, p<.05), lie scale (L) and irregular and life (G) with eat breakfast (p<.01), and aggressiveness (F) with BMI (p<.01), showed highest results in correlation analysis. Fourthly of the Dental technology students' bodily self-reported symptoms, life-style factors which had a statistically significant effect appeared to be regular physical activity (p<.001) and BMI (p<.05), and of the mental self-reported symptoms, causes that did not have statistically significant effect appeared to be regular physical activity (p<.05) and eat breakfast (p<.05). Conclusion: This research concludes that in order to develop correct life-style habits for health promotion and reduce self-reported symptoms related to the Dental Technology students' diseases, education for the recognition of the necessity of self health promotion must be conducted, and a education programme scheme for the Dental technology curriculum for practicing the correct life-style habits in daily life is required.

• 아시안잔디학회지
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• 제23권1호
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• pp.9-22
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• 2009

본 연구는 전 세계적으로 물 부족 현상으로 인한 물의 사용에 대한 관심이 증가함에 따라 네 종류의 난지형 잔디에 각기 다른 비율의 적자란수를 함으로써 식물의 생리학적인 반응, 즉 시각적 품질, 잎의 물 함량, 엽록소 함량, 광합성, 물 이용효율 등이 어떤 반응을 보이는지를 이해하고 그에 따른 상관관계를 분석 해보고자 했다. 결론적으로 20% 정도 적자 관수를 했을 때 식물은 약간의 스트레스를 받는 상태에서도 적자 관수를 하지 않은 식물과 비교했을 때 광합성량의 차이가 없었으며 가장 이상적인 생리학적인 반응을 보였다. 뿌리 발육 부분에 있어서도 적자관수를 통한 건조스트레스는 깊은 뿌리 생육을 촉진하는 관리방법으로 적용되었다. 다음 연구는 잔디가 각기 다른 토양 수분상태에서 일정하게 유지되었을때 지상부와 지하부의 생육에 따른 생리학적인 반응에 대한 연구로 확대되어야 할 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권2호
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• pp.221-227
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• 2004

Nutrient utilization and plasma level of minerals were studied in lactating crossbred cows fed diet supplemented from different source of minerals. Twelve crossbred cows of first to third lactation during their mid stage of lactation were distributed equally under two groups and were fed concentrate mixture, green fodder (para grass) and finger millet straw as per requirement. Cows in group I were fed concentrate mixture supplemented with 1% mineral mixture whereas cows in group II were not supplemented with mineral mixture in the concentrate mixture but were offered additional quantity of green fodder (1 kg DM/cow/day) to compensate for the requirement of minerals. Balance study conducted towards the end of 120 days of feeding experiment indicated that the total dry matter intake in both the groups did not differ significantly. The cows in group II offered additional quantity of green fodder consumed significantly (p<0.05) more of green fodder (5.11 vs. 4.51 kg /animal/day) and the cows in group I consumed significantly (p<0.01) more of finger millet straw (1.71 vs. 0.92 kg/animal/day). The digestibility of major nutrients did not differ between the groups except for ether extract which was significantly (p<0.05) lower in cows fed additional green fodder. The total daily intake of P, Cu, Fe and Co did not differ significantly in both the groups whereas significantly higher intakes of Ca, Mg, Zn and Mn were observed in cows supplemented with inorganic source of minerals. However, supplementation through both the sources could meet the mineral requirement in group I and group II. The gut absorption (%) of all the minerals was comparable between the groups except for Mg which was significantly (p<0.05) higher in cows supplemented mineral mixture. The net retention of all the minerals was significantly more in the group supplemented with inorganic source of minerals. Except for P, Mg and Co the retention as percentage of total intake was comparable for all minerals in both the groups. Irrespective of source of mineral supplementation the average monthly blood plasma mineral levels (Ca, P, Mg, Cu, Zn, Fe) were within the normal range and comparable between the groups. Lower level of Cu observed at the start of the experimental feeding in both the groups increased with the advancement of supplemental feeding. The plasma Zn and Fe content in cows of both the groups did not vary. The blood plasma level of some minerals (Ca, P, Mg and Cu) was significantly higher towards the end of experimental feeding as compared to the initial values due to the reduction in milk yield with advancement of lactation or due to supplemental effect of minerals. It could be concluded that supplementation of minerals through inorganic source is better utilized in terms of retention as compared to green fodder (para grass), which is a moderate source of most nutrients.

• Asian-Australasian Journal of Animal Sciences
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• 제13권5호
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• pp.587-594
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• 2000

At present embryonic stem (ES) cells with confirmed pluripotential properties are only available in the mouse. Recently, we were able to isolate, culture and genetically transform primordial germ cell (PGC)-derived cells from pig embryos and demonstrate their ability to contribute to chimera development in the pig. In order to determine whether the system we developed could be used to isolate embryonic germ (EG) cells from other mammalian species, we placed isolated PGCs from cattle, goats, rabbits and rats in culture. Briefly, PGCs were isolated from fetuses of cow (day 30-50), goat (day 25), rabbit (day 15-18) and rat (day 11-12), and plated on STO feeder cells in Dulbecco's modified Eagle's medium (DMEM): Ham's F10 medium (1:1) supplemented with 0.01 mM nonessential amino acids, 2 mM L-glutamine, 0.1 mM $\beta$ - mercaptoethnol, soluble recombinant human stem cell factor (SCF; 40ng/ml), human basic fibroblast growth factor (bFGF; 20ng/ml) and human leukemia inhibitory factor (LIF; 20ng/ml). For maintenance of the cells, colonies were passed to fresh feeders every 7-10 days. In all species tested, we were able to obtain and maintain colonies with ES-like morphology. Their developmental potential was tested by alkaline phosphatase (AP) staining and in vitro differentiation assay. For genetic transformation, cells were electroporated with a construct containing the green fluorescent protein (GFP) under the control of the cytomegalovirus (CMV) promoter. GFP-expressing colonies were detected in cattle, rabbits and rats. These results suggest that PGC-derived cells from cattle, goats, rabbits and rats can be isolated, cultured, and genetically transformed, and provide the basis for analyzing their developmental potential and their possible use for the precise genetic modification of these species.

• 한국응용곤충학회지
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• 제19권2호
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• pp.85-89
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• 1980

1976년 본해충을 실내사육하면서 얻어진 결과는 다음과 같다. 1. 한 마리당 산란수는 $1202.0{\pm}150.6$개 산란전기간 $9.0{\pm}0.8$일 산란기간 $13.3{\pm}1.2$일 산란후기간은 $1.7{\pm}0.2$일이었고 산란시기는 일몰후였다. 2. 유충은 5령형과 6령형으로 구분할 수 있었고 5령형의 유충기간은 $15.36{\pm}1.59$일 용기간 $7.82{\pm}1.01$일 6령형의 유충기간은 $16.92{\pm}1.08$일 용기간 $8.23{\pm}1.09$일 이였으며 난기간은 2일 자성충 수명은 $21.29{\pm}3.15$일 웅성충은 $19.14{\pm}2.47$일이였다. 3. 령기별 두건은 5령형 $2.77\~21.89mm$ 5령형이 $2.78\~22.38mm$였다. 4. 두건을 logarithum으로 변형했을 때 령기에 따른 두건의 증가는 5령형이 $log \;y=0.228\chi+0.228(r=0.997)$, 6령형이 $log \;y=0.182\chi+0.259(r=0.999)$로 표현되어 고도의 정상관 관계였다.

• Journal of Dairy Science and Biotechnology
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• 제31권1호
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• pp.35-49
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• 2013

The purpose of this study was to develop Mozzarella cheese analogues by using dairy products in the form of WPC 34, WPC 80, whey protein, demineralized whey powder, and lactose powder along with soy milk. Soy milk was separately blended with 5% WPC 34 (A), WPC 80 (B), DWP (C), WP (D), and LP (E) and also with 10% WPC 34 (F), WPC 80 (G), DWP (H), WP (I), and LP (J). Blending of soy milk and whey products showed that increase in the proportions of whey products (WPC 34, WPC 80, DWP, WP, and LP) led to increase in the protein, lactose, and SNF levels of the admixture. A decrease in fat content was observed for all cheeses prepared from mixtures, relative to those for the control cheese. The nitrogen content within analogue samples was higher than that in the control cheese and increased with increase in the proportions of whey products within soy milk. Higher water soluble nitrogen levels were observed in cheese prepared from whey-product-blended soy milk than in the control cheese. The non-protein nitrogen level within the control Mozzarella cheese was significantly lower than that in the Mozzarella analogues, and, in the case of cheese analogues, it increased with increase in the proportion of whey products in soy milk. With regard to the physicochemical and sensory qualities of the Mozzarella cheese analogues and control cheese, the pH of all analogue samples, with the exception of the cheese prepared from group G, was lower than that of the control Mozzarella cheese. Rheological studies showed that the hardness of Mozzarella cheese analogues was lower than that of the control Mozzarella, while the elasticity, cohesiveness, and brittleness of the analogues was higher. The control sample had a higher meltability level than any of the Mozzarella analogues. Mozzarella cheese prepared with the traditional method had higher browning and stretching levels than all the cheese analogues, but a lower oiling-off level.

• Asian-Australasian Journal of Animal Sciences
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• 제23권12호
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• pp.1578-1586
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• 2010

This study estimated the effect of fermented persimmon (Diospros kaki L.) extract (FPE) supplement on feed intake, digestibility, nitrogen (N) balance, and rumen fermentation characteristics in sheep. Five male sheep (Corriedale${\times}$Polwarth) with average body weight of $48.6{\pm}1.3\;kg$ were housed in metabolism crates and assigned to a 5${\times}$5 Latin square design with five consecutive 20-d periods which consisted of 14-d adaptation and 6-d data collection. The sheep were fed ad libitum a diet containing concentrate and rice straw (3:7). The five treatments were FPE supplemented at 0 (Control), 5, 10, 20, and 30 g/kg of concentrate. Intakes of dry matter (DM, p<0.01), organic matter (OM, p<0.01), neutral detergent fiber (NDF, p<0.05), acid detergent fiber (ADF, p<0.05), and nitrogen-free extract (NFE, p<0.01) increased quadratically with increasing intake of FPE supplement and maximized (p<0.05) at 10 g/kg FPE. The digestibilities of DM (p<0.05), OM (p<0.05), crude protein (p<0.01), and NFE (p<0.01) increased quadratically with increasing amount of FPE supplement, and sheep fed 5 and 10 g/kg diets had greater (p<0.05) DM, OM, and NFE digestibilites than the Control treatment. By increasing FPE supplement concentration, N intake (p<0.01) and fecal N (p<0.05) increased linearly, whereas retained N (p<0.05) and retained N ratio (p<0.05) increased quadratically. The retained N was maximized (p<0.05) in sheep fed 5 and 10 g/kg diets. The mean rumen pH was not affected by FPE supplement, but there was a quadratic increase (p<0.05) of mean rumen ammonia N concentration and a linear increase (p<0.01) in mean rumen total volatile fatty acid (VFA) and acetate concentrations. The mean concentration of rumen propionate in sheep fed all FPE supplemented diets was greater (p<0.05) than the Control, but the mean ratios of rumen acetate to propionate in sheep fed 5 and 10 g/kg diets were lower (p<0.05) than that of Control sheep. In conclusion, FPE supplemented at 5-10 g/kg of concentrate improved feed intake, the digestibilites of OM and NFE, N metabolism, and rumen fermentation indices of sheep.

• Asian-Australasian Journal of Animal Sciences
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• 제26권4호
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• pp.537-544
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• 2013

This study examined whether pre-treating palm kernel expeller (PKE) with exogenous enzyme would degrade its fiber content; thus improving its metabolizable energy (ME), growth performance, villus height and digesta viscosity in broiler chickens fed diets containing PKE. Our results showed that enzyme treatment decreased (p<0.05) hemicellulose and cellulose contents of PKE by 26.26 and 32.62%, respectively; and improved true ME (TME) and its nitrogen corrected value ($TME_n$) by 38% and 33%, respectively, compared to the raw sample. Average daily gain (ADG), feed intake and feed conversion ratio (FCR) of chickens fed on different dietary treatments in the grower period were not significantly different. Although there was no difference in feed intake (p>0.05) among treatment groups in the finisher period, ADG of chickens in the control (PKE-free diet) was higher (p<0.05) than in all treatment groups fed either 20 or 30% PKE, irrespective of with or without enzyme treatment. However, ADG of birds fed with 20% PKE was higher than those fed with 30% PKE. The FCR of chickens in the control was the lowest (2.20) but not significantly different from those fed 20% PKE diets while birds in the 30% PKE diets recorded higher (p>0.05) FCR. The intestinal villus height and crypt depth (duodenum, jejunum and ileum) were not different (p>0.05) among treatments except for duodenal crypt depth. The villus height and crypt depth of birds in enzyme treated PKE diets were higher (p<0.05) than those in the raw PKE groups. Viscosity of the intestinal digesta was not different (p>0.05) among treatments. Results of this study suggest that exogenous enzyme is effective in hydrolyzing the fiber (hemicellulose and cellulose) component and improved the ME values of PKE, however, the above positive effects were not reflected in the growth performance in broiler chickens fed the enzyme treated PKE compared to those received raw PKE. The results suggest that PKE can be included up to 5% in the grower diet and 20% in the finisher diet without any significant negative effect on FCR in broiler chickens.

• Asian Pacific Journal of Cancer Prevention
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• 제17권1호
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• pp.37-44
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• 2016

Several infectious agents are considered to be causes of cancer in human, mainly hepatitis B and C viruses, high-risk human pailloma viruses, Helicobacter pylori, Clonorchis sinensis, and Opisthorchis viverrini. Here we described the evident research and the association between Helicobacter spp. and biliary tract cancer particularly cholangiocarcinoma (CCA). Global epidemiological studies have suggested that Helicobacter spp. are possible risk factors for biliary tract diseases. Molecular studies support a linkage of Helicobacter spp. with CCA development. H. pylori, H. bilis, and H. hepaticus, are found in CCA, but the most common species are H. pylori and H. bilis. The type of CCA are associated with Helicobacter spp. include extrahepatic CCA, and common bile duct cancer. Up to the present, however, the results from different regions, materials and methods, sub-sites of cancer, and controls have not been consistent, thus introducing heterogeneity. Therefore, a comparison between co-Helicobacter spp.-CCA in the countries with low and high incident of CCA is required to settle the question. Furthermore, clarifying variation in the role of Helicobacter species in this CCA, including pathogenesis of CCA through enhanced biliary cell inflammation and proliferation, is necessary.

• Asian-Australasian Journal of Animal Sciences
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• 제17권1호
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• pp.73-79
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• 2004

The objective of the present study was to determine whether ${\beta}$1-4 galacto-oligosaccharides (GOS) and Candida kefyr combined with nitrate as manipulators could suppress rumen methanogenesis without nitrate poisoning in sheep. Four rumen fistulated wethers were allocated to a $4{\times}4$ Latin square design. Nitrate (1.3 g $NaNO_3$ $Kg^{-0.75}$body weight) with and without GOS and Candida kefyr were administered into the rumen through fistula as a single dose 30 min after the morning meal. GOS and Candida kefyr were supplemented by sprinkling onto the feed and through rumen fistula, respectively. The four treatments consisted of saline, nitrate, nitrate plus GOS and nitrate plus GOS plus Candida kefyr. Physiological saline was used as the control treatment. Compared to saline treatment, the administration of nitrate alone resulted in a very marked decrease in rumen methanogenesis and an increase in rumen and plasma nitrite production and blood methaemoglobin formation consequently causing a decline in oxygen consumption, carbon dioxide production and metabolic rate. When compared to nitrate alone, the simultaneous administration of nitrate with GOS decreased nitrite accumulation in rumen and plasma and nitrate-induced methaemoglobin, while retaining low methane production. However, GOS could not fully restore metabolic parameters reduced by nitrate. When compared to the simultaneous administration of nitrate with GOS, the simultaneous administration of nitrate with GOS plus Candida kefyr lowered rumen methanogenesis to a negligible level, but did not decrease rumen and plasma nitrite accumulation as well as blood methaemoglobin formation. Thus, these results suggest that combination of nitrate with GOS may be a potent manipulator to suppress rumen methanogenesis with abating the hazards of nitratenitrite toxicity in ruminants.