• 한국가축번식학회지
• /
• 제15권2호
• /
• pp.125-132
• /
• 1991

The studies on the carried out to investigate the effects of capacitation method of spermatozoa on the in vitro fertilization and cleavage rate of bovine follicular oocytes. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluids from the visible of diameter 3~5mm. The follicular oocytes were cultured in TCM-199 medium containing hormones and FCS for 24~48hrs in an incubator with 5% CO2 in air at 38.5$^{\circ}C$ and then matured oocytes were again cultured for 12~18hrs with motile capacitated sperm by preincubation of mKRB, treatment of HIS(high strength ion), Ca-IA(Inophore A), BFF(bovine follicular fluids) and heparin. The results obtained in these experiments were summarized as follows : 1. The in vitro fertilizatin and cleavage rate offollicular oocytes fertilized with capacitated spermatozoas in BO solution by preincubation of mKRB, treatment of HIS, Ca-IA, BFF and heparin method were 53.1%, 33.9%, 50.8%, 48.1%, 58.8% and 28.1%, 17.7%, 26.2%, 22.8%, 32.8%, respectively. And the fertilization and cleavage rate of heparin method was of highest of all. 2. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solutin by both caffeine, BSA and heparin methods were 65.8%, 70.3% and 40.8%, 47.3%, respectively, and those rates were higher treatment of heparin＋BSA, heparin＋caffeine than treatment of heparin. 3. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoa in BO solution with heparin concentrations of 2, 5, 10, 20, 40$\mu\textrm{g}$/ml were 50.0%, 54.7%, 58.1%, 51.7% and 27.9%, 32.8%, 37.1%, 30.0%, respectively. And the fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solution with 10$\mu\textrm{g}$/ml of heparin was the highest of all. 4. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solution containing heparin with caffeine concentraton of 10, 20, 30, 40$\mu\textrm{g}$/ml were 71.4%, 74.3% and 70.6%, 70.0% and 45.7%, 47.3%, 44.1%, 41.4%, respectively. The fertilization and cleavage rate of spermatozoa fertilized in BO solution with caffeine and heparin together(70.3~74.3%) was higher than that of spermatozoa fertilized in BO solution with heparin(58.8%). 5. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solution containing heparin with BSA concentration of 5, 10, 20, 30$\mu\textrm{g}$/ml were 63.6%, 62.9%, 66.7%, 60.3% and 44.1%, 43.5%, 48.5%, 42.7%, respectively. The fertilization and cleavage rate of spermatozoa fertilized in BO solution with BSA and heparin together(60.3~66.73%) was higher than that of spermatozoa fertilized in BO solution with heparin(58.8%).

• 대한한방내과학회지
• /
• 제1권1호
• /
• pp.78-84
• /
• 1976

The SIB ZUN DAE BO TANG is one of the prevailing tonics appeared at first time on TAE PYUNG HOE MIN HAW ZE GUK BANG in Song’s dynasty and have been widely used up to now. To observe the metabolic effects of Thioacetamide from the SIB ZUN DAE BO TANG, the following experiment was performed. 1. The activation of G.O.T. and G.P.T. to blood serum have been decreased when Thioacetamide was administrated into the rabbit. 2. The coefficiency of the SIB JUN DAE BO TANG appears in 3 hours after administrate and it decreases gradually along with it’s time(1st, 2nd, 3rd day) but the coefficiency of the G.P.T. was more apparent on 2nd, 3rd and 5th day. 3. When administrating Thioacetamide, the total amount of cholesterol grows rapidly up to 3rd day and it continuously grows after the 3rd day. But when administrating SIB ZUN DAE BO TANG, the cholesterol amount in blood grows up to 3rd day, but it decreases gradually after it on the other day. The conclusion of the above experiments shows that the prescription (SIB ZUN DAE BO TANG) improves the metabolic deficiency and the recovery and remedy of diseases.

• BMB Reports
• /
• 제45권1호
• /
• pp.32-37
• /
• 2012

Alternative splicing generates several interleukin-6 (IL-6) isoforms; for them an antagonistic activity to the wild-type IL-6 has been proposed. In this study we quantified the relative abundance of IL-6 mRNA isoforms in a panel of mouse tissues and in C2C12 cells during myoblast differentiation or after treatment with the $Ca^{2+}$ ionophore A23187, the AMP-mimetic AICAR and TNF-${\alpha}$. The two mouse IL-6 isoforms identified, IL-6${\delta}$5 (deletion of the first 58 bp of exon 5) and IL-6${\delta}$3 (lacking exon 3), were not conserved in rat and human, did not exhibit tissue specific regulation, were expressed at low levels and their abundance closely correlated to that of full-length IL-6. Species-specific features of the IL-6 sequence, such as the presence of competitive 3' acceptor site in exon 5 and insertion of retrotransposable elements in intron 3, could explain the production of IL-6${\delta}$5 and IL-6${\delta}$3. Our results argued against biological significance for mouse IL-6 isoforms.

• 대한수의학회지
• /
• 제36권3호
• /
• pp.635-646
• /
• 1996

To establish the effective ways to prevent bovine mastitis, the study has been performed to investigate the attributable factors causing down-regulation of immune responses in mammary gland of non-lactating cows. Lymphocytes from peripheral blood and mammary gland secretions(MGS) were obtained from normal healthy cows and mastitic cows, respectively. Cellular immune responses were examined by comparison of proportion of lymphocyte subpopulations using a set of monoclonal antibodies and flow cytometry. The results obtained are as follows. 1. Proportions of peripheral blood lymphocyte subpopulations expressing BoCD2 and BoCD4 molecules were 32.9%, 15.4% in mastitic cows and 43.3%, 28.3% in normal healthy cows, respectively. The ratios of BoCD4 to BoCD8 were 0.76 and 1.47, respectively. 2. Proportions of mammary gland lymphocyte subpopulations expressing BoCD2 and BoCD4 molecules were 18.5%, 8.3% in mastitic cows and 38.2%, 14.2% in normal healthy cows, respectively. The ratios of BoCD4 to BoCD8 were 0.6 and 2.0, respectively. 3. Proportions of T lymphocyte subpopulations from MGS were significantly lower than those from peripheral blood both in mastitic cows and normal healthy cows. However, lymphocyte subpopulations expressing ACT2 and ACT3, which represent activated T suppressor cells, were significantly higher in MGS than those in peripheral blood.

• 한국전기전자재료학회논문지
• /
• 제19권8호
• /
• pp.744-748
• /
• 2006

Emission characteristics of terbium-activated $InBO_3$ under cathode-ray(c.r.) and vacuum ultra violet(v.u.v.) irradiation have been investigated as a functions of norminal compositions and firing conditions. From the x-ray analysis, it was found that some of the synthesized samples contained excess $In_2O_3$ and the amount of the excess $In_2O_3$ varied with the norminal composition and heat treatment conditions. The samples with remanent excess $In_2O_3$ showed lower luminescence intensities than the ones free of excess $In_2O_3$. The phosphors of norminal composition of $InBO_3+10mol%B_2O_3:2mol%Tb$ synthesized at $1250^{\circ}C$ showed excellent CL and PL properties.

• 한국자기공명학회논문지
• /
• 제9권1호
• /
• pp.61-66
• /
• 2005

$^{11}B MAS NMR$ spectra of binary glass system $xV_2O5-B_2O_3$ and ternary glass system $xV_2O5-B_2O_3-yNa_2O$ (x = $V_2O_5 mol%/$B_2O_3$ mol%, y = $Na_2O$ mol$/$B_2O_3$ mol%) were acquired. $BO_3$ units are dominant components in the spectra of $xV_2O_5-B_2O_3$glass systems while both $BO_3$ and $BO_4$ unit appear in comparable amounts in the spectra of $xV_2O_5-B_2O_3-yNa_2O$ glass systems. More $BO_3$ units were monitored for higher $V_2O_5$ contents while more $BO_4$ unit for higher $Na_2O$ contents. Quadrupole parameters such as $e^2qQ$ and $\eta$ obtained form spectral simulation indicate that $e^2qQ$ has a maximum value at x = y 1 and $\eta$ decreases and increases as x or y grows, respectively. Our results suggest that $V_2O_5$ and $Na_2O$ play opposite roles in the ternary glasses.

• 한국전기전자재료학회:학술대회논문집
• /
• 한국전기전자재료학회 1999년도 추계학술대회 논문집
• /
• pp.107-110
• /
• 1999

The ultrasonic spray method was employed to make (Y,Gd)BO$_3$:Eu Phosphor, and its optical properties under 147nm VUV and 254 nm UV excitations were characterized and then compared with that produced by the solid-state reaction. The mixed solution of acetate hydrates of Y, Gd, Eu and boric acid diluted in water or methanol was used as the precursor fur the spray. It was found that (Y,Gd)BO$_3$:Eu phosphor made by this ultrasonic spray had a spherical shape and fine particle size of 1${\mu}{\textrm}{m}$. The crystalline structure for the as-sprayed phosphor was amorphous, but it converted into the same polycrystalline phase of solid state reaction after post heat treatment at 110$0^{\circ}C$ for 2hr. The emitting intensity under VUV and UV excitations for the spray-formed (Y,Gd)BO$_3$:Eu phosphor, however, was inferior to the later one. The excitation spectra were also studied and compared under VUV and UV excitations to explain the change of emitting intensity with Gd substitution in (Y$_{1-x}$ Gd$_{x}$)BO$_3$:Eu Phosphors made by spray and solid state reaction.on.

• 대한한의학방제학회지
• /
• 제15권1호
• /
• pp.37-47
• /
• 2007

許浚(Heo Jun), the best physician on his day, wrote 東醫寶鑑(DongEuiBoGam, 1613) based on his clinical experience as a court doctor and life-long study of over 500 medical books. Now most clinicians consult 東醫寶鑑(DongEuiBoGam) for its practicality. And it is also considered as one of the most valuable medical books for basic medical studies. As 許浚(Heo Jun) mentioned on the 東醫寶鑑(DongEuiBoGam) preface. we should practice flexibility on the interpretation of the medical classics instead of binding ourselves to the precedents. But worship of 東醫寶鑑(DongEuiBoGam) may hinder the studying of new pathways. The treatment and its understandings of a disease should be based on objectivity of observation. Finding the common traits of the recipes in 東醫寶鑑(DongEuiBoGam) is important for the study of 東醫寶鑑(DongEuiBoGam). Accordingly, group synthesis of symptoms and resultant classification of recipes are vital. In this research, symptoms listed in the head part(頭門) of External Shape vol. I(外形篇 卷1) of 東醫寶鑑(DongEuiBoGam) are synthesized into 3 different parts: head-wind syndrome(頭風證), dizziness(眩暈), headache(頭痛). Classification of prescriptions according to these 3 parts is also suggested.

• 한국결정학회지
• /
• 제15권1호
• /
• pp.35-39
• /
• 2004

Two new nickel vanadium borophosphate cluster compounds, $(NH_4)_{10}[Ni(H_2O)_5]_4[V_2P_2BO_{12}]_6{\cdot}nH_2O$ (1) and $(NH_4)_{3.5}(C_3H_{12}N_2)_{3.5}[Ni(H_2O)_6]_{1.25}{[Ni(H_2O)_5]_2[V_2P_2BO_{12}]_6{\cdot}nH_2O$ (2) have been synthesized and structurally characterized. Inter-diffusion methods were employed to prepare the compounds. The cluster anion $[(NH_4)\;{\supset}\;V_2P_2BO_{12}]_6$ is used as a building unit in the synthesis of new compounds containing $Ni(H_2O){^{2+}_5}$ in the presence of pyrazine and 1,3-diaminopropane. Compounds contain isolated cluster anions with general composition ${[Ni(H_2O)_5]_n[(NH_4)\;{\supset}\;V_2P_2BO_{12}]_6}^{-(17-2n)}$ (n = 2, 4). Crystal data: $(NH_4)_{10}[Ni(H_2O)_5]_4[V_2P_2BO_{12}]_6{\cdot}nH_2O$, monoclinic, space group C2/m (no. 12), a = 27.538(2) ${\AA}$, b = 20.366(2) ${\AA}$, c = 11.9614(9) ${\AA}$, ${\beta}$ = 112.131(1)$^{\circ}$, Z = 8; $(NH_4)_{3.5}(C_3H_{12}N_2)_b[Ni(H_2O)_6]_{3.5}{[Ni(H_2O)_5]_2[V_2P_2BO_{12}]_6{\cdot}nH_2O$, triclinic, space group P-1 (no. 2), a = 17.7668(9) ${\AA}$, b = 17.881(1) ${\AA}$, c = 20.668(1) ${\AA}$, ${\alpha}$ = 86.729(1)$^{\circ}$, ${\beta}$ \ 65.77(1)$^{\circ}$, ${\gamma}$ = 80.388(1)$^{\circ}$, Z = 2.

• 한국안광학회지
• /
• 제7권2호
• /
• pp.19-25
• /
• 2002

$SrO-B_2O_3$ 유리들에 같은 양의 $Al_2O_3$와 $SiO_2$를 첨가한 3성분 SrBAl 유리들과 SrBSi 유리들을 제작하여, $^{11}B$ NMR 방법을 이용하여 4배위 붕소 수인 $N_4$, 대칭적 3배위 붕소 수인 $N_{3S}$ 그리고 비대칭적 3배위 붕소 수인 $N_{3A}$를 얻어 구조를 정량적으로 비교 분석하였다. 두 유리 모두에 대해 $BO_3$ 단위의 경우 $Q_{cc}$=2.74MHz, ${\eta}=0.22$이며, $BO_3{^-}$단위의 경우 $Q_{cc}$=2.54MHz, ${\eta}=0.55$ 그리고 $BO_4$ 단위의 경우 $Q_{cc}$=0.60~0.75MHz, ${\eta}{\approx}0.00$이다. SrBAl 유리계에서는 $R_{1st}$($N_4$ 값의 제1변환점)에서의 유리들의 구조는 tetraborate ($[B_8O_{13}]^{-2}$) 단위들과 1차 변형된 diborate($[B_2Al_2O_7]^{-2}$) 단위들로 구성되어졌으며, $R_{max}$($N_4$ 최대인 R)에서 유리의 구조는 diborate($[B_4O_7]^{-2}$) 단위들, metaborate($[BO_2^{-1}]$) 단위들, 1차 변형된 diborate 단위들, 그리고 2차 변형된 diborate($[B_2Al_2O_8]^{-4}$) 단위들로 구성되어졌다. 그리고 3배위인 $AlO_3$ 단위들은 $BO_3$ 단위들보다 SrO로부터 도입된 산소를 우선적으로 배위하여 4배위인 $AlO_4$ 단위들로 전환되었다. 또한, SrBSi 유리계에서는 Si 원자들은 $R{\leq}0.5$ 영역에서는 붕소 망목구조에 기여하지 않으므로 유리 구조는 $SiO_4$ 단위들로 희석(diluted)된 2성분 SrB 유리계와 같은 구조로 구성되어졌으며, $R{\geq}R_{max}$이상의 영역에서는 SrO로부터 도입된 산소에 의해 $SiO_4$ 단위들이 $BO_3{^-}$ 단위들보다 우선적으로 $SiO_4{^-}$ 단위들로 모두 형성되었다. 그리고 $R_{max}$에서 유리의 구조는 diborate 단위들, metaborate 단위들, loose $BO_4([BO_2]^{-1})$ 단위들, 그리고 $SiO_4{^-}([SiO_{2.5}]^{-1})$ 단위들로 구성되어졌다.