• 대한핵의학회지
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• 제1권2호
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• pp.85-97
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• 1967

Histopathological changes of various organs of the mice after intra-peritoneal injections of radioactive iodine ($^{131}I$) were experimentally observed. Sixity healthy female mice, weighing average 25 gm, devided into 6 groups, were used. The various doses of $^{131}I$ were injected intraperitoneally at different intervals. The histopathological changes after these treatments were observed in organs such as thyroids, parathyroids, livers, kidneys and gonads. Following were the results; 1) Thyroid: In the group A given $^{131}I$ with a single dose of $10{\mu}C$ per gm body weight, it was observed that the protoplasms of follicular epithelial cells were destroyed, the nuclei were expanded or dissoluted, showing pyknotic changes of nuclei and vacuolizations of protoplasms. In the group B given $^{131}I$ with a single dose of $5{\mu}C$ per gm body weight, hyperemias, hemorrhages and hyaline degenerations in the whole area were observed. In the group C given $^{131}I$ with 3 doses of $2.5{\mu}C$ per gm body weight every week, the thyroid parenchyms were destroyed and epithelial cells of varing size were observed in the fibrinous tissues. In the group D given $^{131}I$ with 6 doses of $0.5{\mu}C$ per gm body weight every week, some destroyed follicles and new borne follicles were observed. But the histopathological changes resemble the follicles of the normal thyroid gland. In the group E and F given $^{131}I$ with 8 and 10 doses of $0.2{\mu}C\;and\;0.01{\mu}C$ for each group per gm body weight every two days, both pyknotic changes of nuclei and cytoplasmic vacuolizations of the follicular epithelia, hypertrophies of follicles and abnormal irregular follicular structures were observed, and in the group F, lymphocytes appeared around the thyroid glands. 2) Parathyroid: In the group A, hyperemia, proliferations of connective tissues, karyorrhexes and vacuolizations were observed. In other experimental groups, no particular pathological change was observed. 3) Liver: The degnerative changes and acute or chronic inflammatory changes were observed in proportion to the amount of $^{131}I$ injected. Atrophies of the liver cells, dilatations of sinusoids, hyaline degenerations and necrotic pictures were observed. 4) Kidney: In the group A, congestions and infiltrations of mononuclear cells and granulocytes were observed around the cortical arteries, and in the group B, the degenerative changes of cortexes, and, in the group C and D, hydronephrotic changes were observed respectively, and hyaline degenerations were partially observed. 5) Gonad: In the group A, the follicles were degenerated. The ova in the follicles showed irregular figures. The changes in the group B were almost the same as in the group A, but the changes were mild. In the group C, the destructions of whole ova, the hypertrophies of ovarian follicular membranes and pyknotic changes of nuclei were observed. In the group D, the pathological changes were similar to that of group C, but mild in the grade. In the group E, almost none of ovarian follicular fluid was observed, and in the group F, the tissue pictures were almost similar to that of the normal group.

• 대한수의학회지
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• 제32권1호
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• pp.127-134
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• 1992

Present experiments were undertaken in order to clarify the clinico-pathological characteristics of lead poisoning in goats. Twenty goats were divided into three experimental groups(A, B and C) and a control(D). The three experimental groups received diets contaminated artificially with 10(A group), 200(B group) and 1,000(C group) ${\mu}g/$ of lead, for 70 days respectively. The control group received normal diets. Blood samples were collected 1 or 2 weeks interval and were examined for anemia(erythrocyte counts, hemoglobin concentrations and hematocrit values) and lead contents of erythrocyte and serum. Urine samples collected similarly with blood were examined for delta-aminolevulinic acid and lead content. Collected samples were analyzed for lead content by atomic absorption spectrophotometry. From these experiments following results were obtained : In group B and C, marked decreases in body weight and feed intake, and diarrhea were observed from the $30^{th}$ day of experimental periods. The B and C groups showed pronounced anemia(decrease in erythrocyte count, hemoglobin concentration and hematocrit value) from the $21^{st}$ or $42^{nd}$ day. In group B and C, the lead content of erythrocytes was increased significantly from the $28^{th}$ or $14^{th}$ day. The lead content of serum was increased significantly from the $42^{nd}$ or $14^{th}$ day in B and C groups. The lead content of urine was increased significantly from the first day in both B and C groups. The urinary delta-aminolevulinic acid content was increased significantly from $14^{th}$ day in both B and C groups.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.339-339
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• 1994

It has been reported that Indole-3-carbinol (I3C), a naturally occurring compound In cruclferous vegetables, exerts anticarcinogenic activity In several organs In rodents. The modifying effects of I3C were therefore assessed uging a rat multi-organ carcinogenesis model. A total of 100 male Sprague-Dawley rats were divided Into 3 groups. Animals of groups 1 and 2 were sequentially treated with diethylnitrosamine (DEN; 100 mg/kg b.w., i.p.), N-methylnitrosourea (NNU; 20 mg/kg b.w., 4 times for 2 weeks, i.p), and dihydroxy-di-N-propylnitrosauine (DHPN; 0.1% In d.w. for 2 weeks) for 4 weeks (DMD treatment). Animals of groups 1 and 3 were given the diet of 0.25% I3C for 20 weeks after DMD initiation and then were given basal diet for 28 weeks. All animals were sacrificed at week 24 and 52, respectively. I3C has been clearly demonstrated promoting effects on the development of glutathione S-transferase placental form (GST-P) positive hepatic foci at 24 weeks of the experiment. And I3C also exerted promoting potential In the hepatocellular adenoma (4/14; 29%) and the adenoma (7/14; 50%) of the thyroid gland at 52 weeks of the experiment. Therefore, I3C may promote hepatocarcinogenesis and thyroid tumorigenesis in the rat multi-organ carcinogenesis model.

• 대한화학회지
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• 제9권3호
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• pp.142-147
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• 1965

p-Phenylenediamine dihydrochloride의 結晶構造를 X-線 廻折法을 利用하여 解明하였다. 이結晶은 三斜晶係에 屬하며, 空間群은 $C_i1-P{\bar\1}$ 이고, 格子常數는 $a= 4.38{\pm}0.02, b=5.90{\pm}0.02, c=8.76{\pm}0.03{\AA}, {\alpha}=1101{\pm}1, {\beta}=96{\pm}1^{\circ}$이다. 그리고 單位格子속에는 들어있는 化學單位數,Z는 1이다. 各原子의 座標는 a, b 및 c軸에 따른 二次原的인 電子密度의 投影 및 ($F_o-F_c$) 合成에 依하여 얻었다. 이 結晶構造와 이미 硏究된 aliphatic diamine의 hydrogen halide 들의 構造와를 比較하였다.

• 생명과학회지
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• 제21권8호
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• pp.1076-1082
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• 2011

미세소관(microtubule) 위를 이동하는 키네신은 분비소포를 이동시키는 운동단백질이다. KIF5s (KIF5A, KIF5B and KIF5C)는 세포막으로 싸인 각종 세포 내 소기관과 결합하여 미세소관을 따라 목적지까지 이동시킨다는 결과는 알려져 있지만, 어떻게 상대의 cargo를 인식하는지는 밝혀지지 않았다. 본 연구는 KIF5B의 결합 단백질을 동정하기 위하여 효모 two-hybrid system을 사용하여 KIF5B와 특이적으로 결합하는 Myo9b을 확인하였다. Myo9b는 액틴위를 이동하는 운동단백질로 다른 KIF5s들과도 결합함을 효모 two-hybrid assay로 확인하였다. 또한 Myo9s의 GTPase 활성화 단백질(GAP) 영역은 KIF5B와 결합하는데 필수영역임을 확인하였고, 이러한 단백질간의 결합은 Glutathione S-transferase (GST) pull-down assay를 통하여서도 확인하였다. 생쥐의 뇌 파쇄액에 KIF5B들의 항체로 면역침강을 행하여 Myo9s 단백질을 확인한 결과, KIF5s는 Myo9s 단백질과 특이적으로 함께 침강하였다. 이러한 결과들은 kinesin-I는 액틴 결합 운동단백질과 직접 결합함을 보여준다.

• 대한본초학회지
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• 제26권4호
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• pp.31-37
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• 2011

Objectives : Chrysanthemum boreale flower is widely distributed in Korea, Japan, China, and Eastern countries. C. boreale flower is also one of the herbs used for the treatment of various inflammatory disease in Korean Medicine. So, this research was designed to study anti-inflammatory effect of C. boreale flower and its mechanism. Methods : We investigated nitro oxide (NO) and prostaglandin $E_2$ ($PGE_2$) production by ELISA. And expressions of inducible nitric oxide synthase (iNOS), Cyclooxygenase-2 (COX-2) and nuclear factor-${\kappa}B$ P50/65 (NF-${\kappa}B$ P50, NF-${\kappa}B$ P65) were measured in RAW 264.7 murine macrophage cells induced by LPS. Results : MeOH ex., EtOAc fr., $CHCl_3$ fr. and Water fr. of C. boreale flower showed anti-inflammatory effect through inhibition of NO and PGE expression respectively. Among them, EtOAc fr. and $CHCl_3$ fr. inhibited production of NO and $PGE_2$ through inhibition of iNOS and COX-2 expression. And MeOH ex., EtOAc fr. and $CHCl_3$ fr. inhibited translocation of NF-${\kappa}B$ P65, NF-${\kappa}B$ P50 by inhibiting phosphrylation of $I{\kappa}B$. Conclusions : MeOH ex. EtOAc fr, $CHCl_3$ fr., and Water fr. of the C. boreale flower have anti-inflammatory activity.

• 대한화학회지
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• 제42권5호
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• pp.519-525
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• 1998

양이온성 계면활성제인 DPC(dodecylpyridinium chloride)와 다른 양이온성 계면활성제인 DTAB(dodecyltrimethylammonium bromide), TTAB(tetradecyltrimethylammonium bromide) 및 CDEAB(cetyldimethylethylammonium bromide)와의 혼합마이셀화를 연구하기 위하여 전도도법을 이용하였다. DPC/DTAB, DPC/TTAB 및 DPC/CDEAB 혼합계면활성제의 마이셀화에 대한 임계마이셀농도$(CMC^{\ast})$와 반대이온의 결합상수값(B)을 각각 ${\alpha}$1(DPC의 몰분율)의 함수로서 25$^{\circ}C$에서 측정하였으며, 이와같이 측정한 $CMC^{\ast}$와 B 값으로부터 유사상태분리모델을 적용함으로써 여러 가지 열역학적 함수값$(X_{i},\;{\gamma}_{I},\;C_{i},\;a^{M}_{i},\;\beta,\;{\Delta}H_{mix},\;and\;{\Delta}G^{o}_{m})$을 계산하고 분석하였다. 그 결과 DPC/CDEAB 혼합계면활성제가 이상적 혼합마이셀모델에서 가장 큰 벗어남을 나타내었으며 DPC/DTAB 혼합계면활성제는 가장 작은 벗어남을 보였다.

• 한국식품조리과학회지
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• 제9권4호
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• pp.323-328
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• 1993

유지를 가열할때 생성되는 B(a)P 및 기타 PAHs함량과 각 유지의 이화학적 성질변화와의 관계를 비교해 봄으로써 각 유지의 적절한 가열 온도와 시간을 알아보기 위하여 시판 대두유를 $180\pm$5^{\circ}C$$,$200\pm$5^{\circ}C$$, $300\pm$5^{\circ}C$$,에서 50시간 가열한 것을 시료로 취하였다. 이들 각 시료의 B(a)P 및 기타 PAHs함량은 HPLC/UV를 사용하여 측정하였고, 산패와 관련된 이화학적 성질변화를 알아보기 위하여 가열유지의 산값(Acid Value, AV)과, 공액이중 산값(Conjugated Diene Value, CDNV)을 측정하였다. 그 결과는 다음과 같았다. 1. 시료로 사용한 대두유의 B(a)P 및 PAHs함량은 Pyr 1.093, B(a)P 0.986, Ch 1.147, DMBA 1.082, B(e)P 0.664, Per 1.135, B(a)P 0.146, DBA 1.053, 3-MC 0.055 $\mu\textrm{g}$/kg 이었다. 대두유를 $180\pm$5^{\circ}C$$, 에서 10,20,30,50시간 가열했을 때 B(a)P함량은 각각 0.391, 0.692, 0.451, 0.372$\mu\textrm{g}$/kg이었으며 $200\pm$5^{\circ}C$$, 와 $300\pm$5^{\circ}C$$,에서 가열했을 때 B(a)P함량은 각각 10시간일때 0.844와 0.466, 20시간일때 0.512와 0.706, 30시간일때 0.479와 0.607, 50시간일때 0.367과 0.247 $\mu\textrm{g}$/kg이었다. 2. 대두유를 10,20,30,50시 간 가열했을 때 산값은 180$\pm$5$^{\circ}C200\pm$5^{\circ}C$$,에서는 0.26, 0.26, 0.27, 0.33이었고, 200$\pm$5$^{\circ}C$에서는 0.23, 0.21, 0.21, 0.52였으며 $300\pm$5^{\circ}C$$,에서는 0.47, 1.57, 3.90, 6.42였다. CDNV는 $180\pm$5^{\circ}C$$,에서는 0.67,0.76,0.99,1.04였으며, $200\pm$5^{\circ}C$$,$^{\circ}C$에서는 0.39, 0.49, 3.27, 3.89였고 $300\pm$5^{\circ}C$$,에서는 0.65, 2.I5, 3.00, 3.88이었다. 3. 대두유를 가열했을 때 B(a)P의 생성과 산패도의 변화와의 관계를 볼때 B(a)P함량은 $180^{\circ}C$에서는 20시간가열시 0.692$\mu\textrm{g}$/kg, $200\pm$5^{\circ}C$$,에서는 10시 간 가열시 0.844$\mu\textrm{g}$/kg, $300\pm$5^{\circ}C$$,에서는 20시간 가열시 0.706$\mu\textrm{g}$/kg으로 가장 높게 생성된 후 서서히 감소하였다. 그러나 산값과 공액이중산값은 계속 증가하는 양상을 나타냈다. 즉 B(a)P생성과 산패도 변화사이에는 일정한 관계를 나타내지는 않았다.

• 대한화학회지
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• 제11권1호
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• pp.12-16
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• 1967

?織物上의 Chlorantine Fast Red 5B(C.I. Direct Red 81)의 照光으로 因한 褪色을 檢討하여 同染料의 CF曲線의 기울기는 異例的으로 負의 기울기를 나타내는 反面 CFG 曲線의 기울기는 正의 기울기를 나타낸다는 것을 밝혀내고, 同染料의 CF曲線의 기울기가 負로 나타남에도 不拘하고 CFG曲線의 기울기는 正으로 나타나게 되는 理由를, Weber-Fechner의 法則이 染色物의 視覺에 依한 褪色判定에 作用하는 때문인 것으로 보고, 視覺判定에 依할 때의 染料의 褪色量($F_v$)은 $F_v=[logC_0-log(C_0-C_0F_t/100)]{\times}100/(logC_0+b)$ 但 $F_t$; t時間 照光後의 染料의 褪色量, $C_0$; 染料의 初濃度, b; 定數으로 表示할 수 있다는 것을 明白히 하였다. 아울러 染料濃度 增加에 따르는 日光堅牢度 上昇의 原因으로서 染料의 集合效果外에, 染料濃度가 視覺判定에 依한 褪色判定結果에 미치는 影響도 考慮해야 할것임을 明白히 하였다.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 2004년도 추계학술발표회 발표논문집
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• pp.225-226
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• 2004