• 한국식품영양과학회지
• /
• 제29권5호
• /
• pp.893-899
• /
• 2000

Oil emulsion에 느릅나무 근피(한국산, 중국산), 수피(한국산) 각 추출물 0.05% 첨가에 의한 항산화 작용의 실험 결과는 다음과 같다. 활성 산소종, 금속이온 참가에 따른 지방 산화 억제능은 근피, 수피 각 용매 추출물의 $H_2O_2,-OH,\;KO_2$에 대한 포집능은 모두 우수하여 높은 항산화 효과를 나타내었으며, 각 추출물과 $Fe^{2+}$ ion과 $Cu^{2+}$ ion도 강한 활성 유리 라디칼 포집능으로 우수한 항산화 효과를 보였다. 지방 산화를 촉진하는 nonheme iron 함량에서 $Fe^{2+}$ 함량과 total iron 함량은 근피(중국산) 에탄올, 메탄올 추출물이 가장 높은 함량을 나타내었고, 한국산 근피, 수피의 에탄올, 메탄올 그리고 부탄올 추출물군에서 80% 이상의 높은 활성을 보였고, 물 추출물군의 경우 20~55%의 범위로 낮은 효과를 보였다. SOD 유사활성은 근피(중국산) 부탄올 추출물, 수피(한국산) 물 추출물에서 가장 높은 활성능을 보였다. 아질산염 소거능의 경우도 근피, 수피 에탄올, 메탄올 그리고 부탄올 추출물군 모두 우수한 소거능을 보였고, 특히 pH 1.2에서는 가장 높은 아질산염 소거능을 보였으며, pH 6.0으로 갈수록 급격히 감소하였다.

• Journal of Plant Biotechnology
• /
• 제36권2호
• /
• pp.149-156
• /
• 2009

Application of exogenous polyamines (PAs) reduced the paraquat (PQ)-induced cotyledon injuries in radish seedling plants with 1 mM spermidine (Spd) being the most effective protectant. PQ injury symptoms in the cotyledons, e.g., large accumulation of $H_2O_2$, and losses of fresh weight, chlorophyll, and proteins, were significantly alleviated. Likewise, analysis of $H_2O_2$-scavenging enzymes such as catalase (CAT) and guaiacol peroxidase (GPX) showed that pretreatment with Spd among PAs remarkably increased total CAT activity and strongly retarded PQ-induced rapid decline in total GPX activity. In a native gel assay, one CAT isozyme (CAT1) and two GPX isozymes (GPX1 and a newly synthesized GPX isozyme) proved to be more responsible for PQ tolerance, as manifested by the strong increases in their activities by Spd pretreatment. Based on these results, we can suggest that PAs (especially 1 mM Spd) may function as antioxidant protectors by invoking CAT and GPX enzymes which control the endogenous $H_2O_2$ level in radish cotyledons exposed to PQ.

• Fisheries and Aquatic Sciences
• /
• 제13권1호
• /
• pp.26-35
• /
• 2010

In our previous study, we preliminarily demonstrated that Celluclast and Neutrase extracts exhibited the strongest $H_2O_2$-scavenging activities among five carbohydrases (Viscozyme, Celluclast, Termamyl, Ultraflo and AMG) and five proteases (Kojizyme, Alcalse, Flavourzyme Protamex and Neutrase) extracts. Thus, Celluclast and Neutrase extracts were selected for use in further experiments and were separated into four different molecular weight fractions (<5, 5-10, 10-30 and >30 kDa). Among them, the 5-10 kDa fraction showed the highest $H_2O_2$-scavenging activity. The 5-10 kDa fraction also strongly enhanced cell viability against $H_2O_2$-induced oxidative damage. Furthermore, the fraction reduced the proportion of apoptotic cells induced by $H_2O_2$, as demonstrated by decreased sub-G1 hypodiploid cells and decreased apoptotic body formation by flow cytometry. These results indicated that the 5-10 kDa fraction of the Celluclast and Neutrase extracts from S. coreanum exhibited strong antioxidant activity over $H_2O_2$-mediated cell damage in vitro.

• 대한약리학회지
• /
• 제31권3호
• /
• pp.345-353
• /
• 1995

Harmaline을 포함한 ${\beta}-Carboline$ 알카로이드들은 마이크로조움의 효소성 또는 비효소성 지질 과산화를 억제한다고 제시되고 있으나, 이들의 항산화 작용기전은 분명하지 않다. 본 연구에서는 $Fe^{2+}$와 $H_2O_2$에 의한 hyaluronic acid, 지질과 콜라젠의 산화성 손상에 있어 harmaline과 harmalol의 항산화 능력을 관찰하였다. 또한 반응성 산소대사물에 대한 이들의 제거작용을 조사하였다. Harmaline, harmalol, superoxide dismutase, catalase와 DMSO는 $Fe^{2+}$와 $H_2O_2$에 의한 hyaluronic acid의 변성과 $Fe^{2+}$에 의한 지질 과산화를 억제하였다. 이들 반응에서 DABCO는 hyaluronic acid의 변성을 억제하였으나 지질 과산화에 영향을 나타내지 않았다. ${\beta}-Carboline$은 $Fe^{2+}$, $H_2O_2$와 ascorbic acid에 의한 cartilage collagen의 변성을 억제하였다. Superoxide dismutase에 의하여 억제되는 $Fe^{2+}$의 자가산화에 따른 ferricytochrome c의 환원은 harmaline과 harmalol의 영향을 받지 않았다. 또한 이들은 $H_2O_2$에 대하여 분해작용을 나타내지 않았다. $Fe^{2+}$와 $H_2O_2$의 존재하에서 OH 생성은 harmaline, harmalol과 DMSO에 의하여 억제되었다. Harmaline과 harmalol은 반응성 산소대사물인 OH 과 아마도 철이온-산소 복합체에 대한 제거작용으로써 $Fe^{2+}$와 $H_2O_2$에 의한 hyaluronic acid, 지질과 콜라젠의 산화성 손상을 억제하고, 항산화 능력을 나타낼 것으로 추정된다.

• ALGAE
• /
• 제21권1호
• /
• pp.149-156
• /
• 2006

The aim of the present study was to evaluate the antioxidant activity of red algae in Jeju Island. The algal extracts were obtained with MeOH and fresh water at 20 and 70°C, and screened for antioxidant activities using hydroxyl radical (HO·), superoxide anion (O2–), hydrogen peroxide (H2O2) and DPPH free radical scavenging assays. Among them, Gracilaria verrucosa methanolic extract at 20°C (20ME, 96.85%), G. textorii aqueous extract at 20°C (20AE, 88.01%), Grateloupia filicina 20AE (85.35%), and Polysiphonia japonica 20ME (94.92%) exhibited the highest scavenging activities against HO·, O2–, H2O2, and DPPH free radicals, respectively. Moreover, P. japonica (20ME and 70ME) is correlated between DPPH free radical scavenging activity and polyphenolic contents. These results indicate that some red algae in Jeju Island could be potential candidates for development of antioxidants.

• ALGAE
• /
• 제20권3호
• /
• pp.251-260
• /
• 2005

Antioxidative activities of Chlorophyta and Phaeophyta in Jeju Island were measured by superoxide anion ($O_2^{{\cdot}-}$), hydroxyl radical ($HO^{\cdot}$), hydrogen peroxide ($H_2O_2$) and DPPH free radical scavenging assays. Methanolic and aqueous extracts of the seaweeds were prepared at both temperatures, higher (70$^{\circ}C$) and room temperature (20$^{\circ}C$), and screened for the construction of an extract bank from seaweeds in Jeju Island. A variety of extracts showed positive effect against reactive oxygen species (ROS). Especially, Sargassum thunbergii methanolic extract at 70$^{\circ}C$ (70ME, 97.41%), S. fulvellum methanolic extract at 20$^{\circ}C$ (20ME, 84.66%), Codium fragile aqueous extract at 70$^{\circ}C$ (70AE, 96.61%) and S. thunbergii 20ME (97.44%) exhibited the highest scavenging activities against $O_2^{{\cdot}-}$, $HO^{\cdot}$, $H_2O_2$ and DPPH free radicals, respectively. Total phenolic contents also examined but did not show a positive correlation with ROS scavenging abilities (except for a few extracts). These results indicate that further investigation is needed to identify and purify the responsible antioxidative components.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2022년도 추계학술대회
• /
• pp.31-31
• /
• 2022

Plant-derived antioxidants are used as a healthy diet and are known to inhibit various human diseases. In this study, we investigated free radical scavenging and antioxidant activity of extracts from three plants (Ammannia multiflora, Ammannia coccinea and Salix gracilistyla) with the most DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity from 196 plant extracts inhabiting along Nakdong River in Republic of Korea. The three extracts also have strong total antioxidant activity. Moreover, the extracts inhibited hydrogen peroxide (H₂O₂)-induced reactive oxygen species production and depolarized mitochondrial membrane potential in RAW264.7 macrophages. In zebrafish larvae, 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) fluorescent intensity, induced by H₂O₂, was markedly reduced by the extracts of A. multiflora, A. coccinea and S. gracilistyla. Meanwhile, the extracts were upregulated Nrf2 and HO-1 expression, and an HO-1 inhibitor reversed the extract-induced oxidative responses both in vivo and in vitro. The data suggest that the extracts of A. multiflora, A. coccinea, and S. gracilistyla exert potential free radical scavenging and antioxidant capacities both in vivo and in vitro by activating the Nrf2/HO-1 signaling pathway.

• 한국식품영양과학회지
• /
• 제29권1호
• /
• pp.106-110
• /
• 2000

We investigated the active oxygen scavenging activity and active compound from Humulus japonicus. The ethyl acetate and butanol fraction exhibited strong scavenging effects on hydroxyl radical. Furthermore active compound was isolated and purified using Amberlite XAD-2 column chromatography and preparative HPLC from ethyl acetate fracton, and major compound was identified as a luteolin-7-O-$\beta$-D-glucoside by the MS, UV, 1H-NMR and 13C-NMR analyses. Luteolin-7-O-$\beta$-D-glucoside exhibited strong scavenging effect on active oxygens such as superoxide radical, hydroxyl radical and hydrogen peroxide.

• 대한한방내과학회지
• /
• 제25권2호
• /
• pp.268-276
• /
• 2004

Objective : Peroxynitrite $(ONOO^-)$, formed from the reaction of $O_2^-$ and NO, is a cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer's disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate scavenging activities of $ONOO^-$ and its precursors. NO and $O_2^-$ and its scavenging mechanism using fluorescent probes, DCFDA, DAF-2 and DHR 123.. Methods : Psoralea corylifolia was crushed. The crushed Psoralea corylifolia was extracted 3 times, each time with 3 volumes of methyl alcohol at $60^{\circ}C$ for 24 h. The extract was filtered and evaporated under reduced pressured using a rotary evaporator to yield 16g. This was done to investigate scavenging activities of $ONOO^-$, NO, $O_2^-$ and its scavenging mechanism using fluorescent probes, DCFDA, DAF-2 and DHR 123. Results : After Psoralea corylifolia was added authentic $ONOO^-,\;{\cdot}\;O_2^-$ and NO was markedly scavenged. Also, $ONOO^-$ induced by $O_2^-$ and NO (these derived from SIN-1) was inhibited. The data showed a decrease in $ONOO^-$ mediated nitration of tyrosine through electron donation after Psoralea corylifolia was added. Data showed a dose-dependent correlation with inhibition of nitration of bovine serum albumin induced by $ONOO^-$, Furtheremore, LPS-induced ROS and RNS generation was blocked. Conclusions: These results suggest potential for use of Psoralea corylifolia as an effective $ONOO^-$ scavenger to counter the aging process and age-related diseases.

• 한국식품영양과학회지
• /
• 제26권5호
• /
• pp.814-821
• /
• 1997

Kimchi is composed of many ingredients such as Chinese cabbage, garlic, ginger, and red pepper and fermented fish extract. Some of them were known to have antioxidative activities due to their scavenging effect against reactive oxygen species(ROS). To study the health effects of kimchi on human skin cells, keratinocyte(A431, epidermoid carcinoma, human) and fibroblast(CCD-986SK, normal control, human) were cultured in oxidative stress condition provoked by paraquat, a superoxide anion generator, and hydrogen peroxide in the absence and presence of kimchi extract. The survival rate of keratinocyte was greatly reduced when exposed over 1mM concentration of hydrogen peroxide($H_{2}O_{2}$), but cytotoxicity of $H_{2}O_{2}$ was significantly reduced by kimchi extracts on cells. Especially 2 week-fermented kimchi decreased remarkably the cytotoxicity by $H_{2}O_{2}$ to keratinocyte cells. Over 1mM of paraquat concentration showed strong cell toxicity on keratinocyte, but the extracts from kimchi fermented for 1, 2 and 3 weeks showed protective effects in order. Fibroblast cells were significantly affected by $H_{2}O_{2}$ as were keratinocyte cells. Although almost all extacts of kimchi of different fermentation periods showed protective effect against cell killing at 0.5mM concentration of $H_{2}O_{2}$ week-fermented kimchi extract showed the strongest protective effect on fibroblast cells treated with 1mM $H_{2}O_{2}$ for either 1 day or 4 days. However most of kimchi extracts showed weak preventive effect or no effect on oxidative stress produced by paraquat. In conclusion, 2 week-fermented kimchi extract seems to have the best potential in preventing skin cells against oxidative damage which might be related to their scavenging effects of kimchi components produced during their fermentation process.