• The Korean Journal of Food And Nutrition
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• v.32 no.5
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• pp.462-472
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• 2019

The purpose of this study was to demonstrate the quality characteristics of Brassica juncea cultivated in Jeongseon (BJJ), South Korea. We analyzed the nutritional components and antioxidant activity of BJJ. As a result of the free sugar analysis, the contents of glucose and fructose in BJJ were $0.29{\pm}0.02g/100g$ and $0.10{\pm}0.00g/100g$, respectively. The major fatty acids were palmitic acid, octadecenoic acid and stearic acid. The palmitic acid was the highest at 31.22% of all fatty acids. The major minerals were identified as Ca, P, K, Mg and Na. The contents of vitamin $B_1$, vitamin $B_2$, vitamin $B_6$, vitamin C and vitamin E in BJJ were $0.02{\pm}0.00mg/100g$, $0.087{\pm}0.01mg/100g$, $0.02{\pm}0.00mg/100g$, $0.56{\pm}0.06mg/100g$ and $0.20{\pm}0.03mg\;{\alpha}-TE/100g$, respectively. As a result of the free amino acid analysis, total amino acid contents in BJJ were $2,801.21{\pm}115.38mg/100g$. L-proline content was the highest ($744.30{\pm}119.06mg/100g$) in BJJ. BJJ extract inhibits reactive oxygen species production in 3T3-L1 adipocytes. Also, BJJ extract exhibits a protective effect on oxidative stress in $H_2O_2$-induced human dermal fibroblast. These results indicate that BJJ comprises various valuable nutrients which can be used as functional food ingredients.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.209-210
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• 2016

우리나라의 연평균강수량은 약 1362 mm이며, 총강수량의 약 30% 이상이 증발산을 통해 손실되고 있다고 추정되어지고 있다. 증발산은 물 수지 분석에 있어 매우 중요한 성분이며, 많은 부분을 차지하지만 다른 요인들에 비해 직접적인 관측이 어려워 과거에는 경험식을 사용하거나 단순하게 가정에 의해 결정해 왔다. 또한 기상자료로부터 증발산량을 추정하거나 증발접시나 추정식으로 잠재증발산을 추정하고 있다. 또한 최근 기후변화의 가속화에 따른 홍수의 가뭄의 강도와 빈도가 높아지고 있으며, 이에 따라 수자원 관리에 있어서 기초수문조사 항목에 많은 변화를 요구하고 있다. 그 결과 2007년 4월 하천법 개정으로 증발산량 및 토양수분량이 기초수문조사 항목으로 추가되었으며, K-water 연구원에서는 용담시험유역에 플럭스타워를 설치하였고 현재 운영 중에 있다. 덕유산 플럭스타워는 용담시험유역 내에 위치한 금강 수계 구량천 상류부의 덕곡제 유역 내에 설치하였으며, 2011년 4월부터 실제 증발산량을 관측하고 있다. 동경 $127^{\circ}$42'23" ~ $127^{\circ}$44'53", 북위 $35^{\circ}$50'47" ~ $35^{\circ}$52'50"사이로 중부지방에 위치한 유일한 증발산관측 타워이다. 유역 면적은 9.27 km2으로 유로연장 3.48 km, 유역 평균폭 2.66 km, 형상계수는 0.77이며, 덕곡제플럭스 타워 주변의 토지이용은 대부분 산림으로 구성되어 있으며, 침활 혼효림과 낙엽송림으로 임상 분포가 이루어져 있다. 주요 관측기기로는 3차원 풍향 풍속계, $CO_2/H_2O$ 기체분석기, 순복사 측정 센서, 지중열플럭스 측정 센서 등이 있다. 2011년부터 측정된 자료를 바탕으로 에디공분산 방법을 이용하여 증발산량을 측정하였으며, 30분간의 데이터 18,000개 중 취득률 90 % 이상의 데이터를 대상을 분석을 실시하였다. 2011 ~ 2015년도 증발산량 분석 결과는 아래의 표와 같다. 증발산의 패턴은 1월부터 서서히 증가하지만 활발하지는 않고, 4월부터 매우 활발해져 8월에 최대치에 이른다. 10월부터 증발산량은 급격히 감소하기 시작하며 11, 12월에는 증발산이 거의 발생하지 않는 공통적인 경향을 보였다. 2013년 8, 9월은 다른 해와 다른 경향을 보이고 있는데, 이는 2013년 8, 9월에 강우가 많이 발생하여 증발산량이 감소하였기 때문으로 판단된다. 2015년 8월은 다른 년도와 비교했을 때, 매우 높은 증발산량을 보이는데 이는 2015년 8월에 많은 강우에도 식생이 활발하게 작용하였기 때문으로 판단된다.

• Korean Journal of Food Science and Technology
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• v.51 no.5
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• pp.473-479
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• 2019

Gryllus bimaculatus (GB) has recently been registered as a food variety in Korea. In the present study, we prepared protein hydrolysates from GB and evaluated their antioxidant capacity. Protein hydrolysates were prepared from dried GB using enzymatic hydrolysis using five different proteases, and protein hydrolysates showing high hydrolysis value (alcalase, flavourzyme, and neutrase) were separated further into fractions ${\leq}3kDa$ and then lyophilized. Based on $RC_{50}$ values of hydrolysates (${\leq}3kDa$) obtained from four different antioxidant analyses, the flavourzyme hydrolysates showed relatively high levels of antioxidant capacity among the three hydrolysates, and in particular, it showed considerably strong antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays. The flavourzyme hydrolysate also significantly inhibited peroxidation of linoleic acid. These results suggest that protein hydrolysates from GB represent potential sources of natural antioxidants. Our current studies are focused on identification of active peptides from the flavourzyme hydrolysate.

• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.92-96
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• 2015

It is known that silk protein supports effectively proliferation of cell such as insect cell and hybridoma cell. Although there are many varieties of Bombyx mori silkworm, the effect of silkworm varieties on cell proliferation has not been considered in detail. We studied that characteristics of silk cocoon obtained from Baegokjam, Kumokjam, Daeseongjam silkworm varieties and whether silk protein affected cell proliferation or not. Silk sericin was prepared under high temperature and high pressure condition. Silk fibroin was prepared using $CaCl_2:H_2O:EtOH$ with different dissolution time. As a result, there are differences in silk cocoon from different silkworm varieties about cell proliferation. The proliferation was accelerated in the presence of Baegokjam silk sericin and Kumokjam silk fibroin with 5hr dissolution time. We expect that silk proteins could be a preferable culture medium supplement for stimulating the proliferation of cell. Then, this results suggest silk as a new material for medium supplement replacing with fetal bovine serum.

• Journal of The Korean Society of Agricultural Engineers
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• v.63 no.6
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• pp.1-16
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• 2021

Global warming due to climate change is expected to significantly affect the hydrological cycle of agriculture. Therefore, in order to predict the magnitude of climate impact on agricultural water resources in the future, it is necessary to estimate the water demand for irrigation as the climate change. This study aimed at evaluating the future changes in water demand for irrigation under two Shared Socioeconomic Pathways (SSPs) (SSP2-4.5 and SSP5-8.5) scenarios for paddy rice in Gimje, South Korea. The APEX-Paddy model developed for the simulation of paddy environment was used. The model was calibrated and validated using the H₂O flux observation data by the eddy covariance system installed at the field. Sixteen General Circulation Models (GCMs) collected from the Climate Model Intercomparison Project phase 6 (CMIP6) and downscaled using Simple Quantile Mapping (SQM) were used. The future climate data obtained were subjected to APEX-Paddy model simulation to evaluate the future water demand for irrigation at the paddy field. Changes in water demand for irrigation were evaluated for Near-future-NF (2011-2040), Mid-future-MF (2041-2070), and Far-future-FF (2071-2100) by comparing with historical data (1981-2010). The result revealed that, water demand for irrigation would increase by 2.3%, 4.8%, and 7.5% for NF, MF and FF respectively under SSP2-4.5 as compared to the historical demand. Under SSP5-8.5, the water demand for irrigation will worsen by 1.6%, 5.7%, 9.7%, for NF, MF and FF respectively. The increasing water demand for irrigating paddy field into the future is due to increasing evapotranspiration resulting from rising daily mean temperatures and solar radiation under the changing climate.

• Journal of Climate Change Research
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• v.3 no.4
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• pp.235-243
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• 2012

Mobile sources are one of the most significant contributors to the inventory of greenhouse gas (GHG). The administration in Korea has set a goal of cutting GHG emissions of vehicles by 34.3% compared to Business As Usual (BAU) by 2020. To achieve this goal, GHG emission standards for vehicles have been applied since 2012, and now light-duty trucks are under consideration to be included to the vehicle types that will be regulated in the new version of GHG emission standards. Therefore, this study focuses on analyzing characteristics of exhaust GHGs (CO2, CH4, and N2O) emissions of diesel light-duty trucks according to their various driving modes. GHGs emissions of diesel light-duty trucks reduced in inverse proportion to the speed of the vehicles. GHGs emissions from the combined mode were 8% and 14% lower than those from the CVS- 75 and NEDC modes, respectively.

• Journal of Practical Agriculture & Fisheries Research
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• v.22 no.1
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• pp.43-53
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• 2020

The test conducted in this study was carried out for three years from 2013 to 2015 to investigate the optimal level of seeding amount and nitrogen fertilizer application to cultivate and produce the seeds of rye in Jangsu province, Jeollabuk-do. The variety of forage rye used in this experiment was 'Gogu', developed by National Institute of Crop Science, Suwon, Korea in 2004. The seeding rate was four levels (3, 5, 7 and 9 kg 10a^-1, respectively) while nitrogen fertilized with respective amounts of 0, 3, 6, and 9 kg 10a^-1. In Jangsu, the number of spike per m² and lodging ratio were increasing, while fertility rate, 1 liter weight, 1000-grain weight and seed productivity were decreased by increasing of seeding rate from 3 kg 10a^-1 to 9 kg 10a^-1. There was an increase in the number of spike per m², culm lengh, the number of grain per spike, lodging ratio and seed productivity, while productiove tiller, 1 grain weight, and 1 liter weight were decreased by increasing nitrogen fertilizer level from 3kg 10a^-1 to 9 kg 10a^-1. The best seeding rate and nitrogen fertilizer level for rye seed production were 5 kg and 6 kg 10a^-1, respectively, considering plant lodging, seed and fertilizer reduction and the prevention of pollution by excess fertilization.

• Journal of Microbiology and Biotechnology
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• v.34 no.3
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• pp.606-621
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• 2024

This study evaluated the hepatoprotective effect of fermented Protaetia brevitarsis larvae (FPB) in ethanol-induced liver injury mice. As a result of amino acids in FPB, 18 types of amino acids including essential amino acids were identified. In the results of in vitro tests, FPB increased alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) activities. In addition, FPB treatment increased cell viability on ethanol- and H₂O₂-induced HepG2 cells. FPB ameliorated serum biomarkers related to hepatoxicity including glutamic oxaloacetic transaminase, glutamine pyruvic transaminase, total bilirubin, and lactate dehydrogenase and lipid metabolism including triglyceride, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol. Also, FPB controlled ethanol metabolism enzymes by regulating the protein expression levels of ADH, ALDH, and cytochrome P450 2E1 in liver tissue. FPB protected hepatic oxidative stress by improving malondialdehyde content, reduced glutathione, and superoxide dismutase levels. In addition, FPB reversed mitochondrial dysfunction by regulating reactive oxygen species production, mitochondrial membrane potential, and ATP levels. FPB protected ethanol-induced apoptosis, fatty liver, and hepatic inflammation through p-AMP-activated protein kinase and TLR-4/NF-κB signaling pathways. Furthermore, FPB prevented hepatic fibrosis by decreasing TGF-β1/Smad pathway. In summary, these results suggest that FPB might be a potential prophylactic agent for the treatment of alcoholic liver disease via preventing liver injury such as fatty liver, hepatic inflammation due to chronic ethanol-induced oxidative stress.

• Journal of Animal Science and Technology
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• v.45 no.2
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• pp.319-326
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• 2003

Angiotensin-I converting enzyme(ACE)inhibitor was isolated from beef by-products. The beef by- product hydrolysates prepared with various proteases were tested for the inhibitory effects against ACE. The proteases used were proteinase A from bakers yeast, protease type ⅩIII fungal and thermolysin. The maximum inhibitory effect was observed after hydrolysis for 12hrs(beef heart) and 24hrs(beef spleen), respectively. After gel filtration, IC50 value was 0.37mg/ml in beef heart and 1.84mg/ml in beef spleen. After RP-HPLC, the IC50 value of peak 1, peak 2, peak 3 and peak-4 were 0.28mg/ml, 0.26mg/ml, 0.25mg/ml and 0.35mg/ml, respectively. In the results of amino acid composition of peak 1, peak 2, peak 3 and peak 4, it was observed that peak 1 was consisted mainly of glycine and methionine, peak 2 was proline, cystine and methionine, peak 3 was proline and peak 4 was alanine, methionine and leucine. In conclusion, beef heart hydrolysate treated with thermolysin+ proteinase A was shown to have the highest inhibitory effect for 12hrs incubation at 37$^{\circ}C$.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.33 no.4
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• pp.263-267
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• 2007

Parabens are used in nearly all types of cosmetics and toiletries because they are formulated well and have broad spectrum of activity, interness, low costs and excellent chemical stability in relation to pH. 2-phenoxyethanol and chlorphenesin are common preservatives which are usually used in combination with parabens in cosmetics. Toxicity of parabens is generally low but application of parabens to damaged or broken skin has resulted in sensitization. Moreover, the possibility of their estrogenic potential, anesthetic effects and reproductive toxicity has been reported. Consequently there are some regulations in use of parabens. And the maximum permitted concentrations of chlorphenesin and 2-phenoxyethanol in cosmetic products are authorized by the same reasons. So it is important to control and estimate the amount of parabens in products. In this article, we proposed a valid method for the simultaneous determination of 8 preservatives including parabens in a short time using ultra performance liquid $chromatography^{TM}\;(UPLC^{TM})$. Separation of eight components was achieved in less than 10 min and resolutions were reasonable (USP resolution ${\geqq}\;2$). And limit of detection and quantification were evaluated. The method was suitably validated for specificity, linearity, precision (repeatability, intermediate precision) and accuracy for assay (recovery) based on International conference on harmonisation (ICH) guideline. The method was applicable to analysis of preservatives in cosmetic products.