• Title/Summary/Keyword: $H_2O $

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Studies on the trehalose and other constituents of Saccharomyces cerevisiae Rasse O cultured on various molasses media (Saccharomyces cerevisiae Rasse O의 배양조건과 trdhalose를 중심으로한 균체성분과의 관계에 대하여)

  • 황규찬
    • Korean Journal of Microbiology
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    • v.8 no.2
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    • pp.85-89
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    • 1970
  • Effects of the sugar content in molasses media and pH on cell constituents of produced yeast adopting Saccharomyces cerevisiae Rasse O as a seed organism were studied, and following results were obtained. 1. Trehalose accumulation of the yeast was reduced at lower range of pH, however protein was increased. 2. Trehalose content of the yeast enriched by feeding increased sugar at suitable pH. 3. There was no significant increase of thehalose content in the cell by feeding concentrated molasses at lower range of pH.

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Low Temperature Sintering Properties of the $0.6TiTe_3O_8-0.4MgTiO_3$ Ceramics with Sintering Adds (소결조제 첨가에 따른 $0.6TiTe_3O_8-0.4MgTiO_3$ 세라믹스의 jdhs 소결 특성)

  • Kim, Jae-Sik;Ryu, Ki-Won;Koh, Jung-Hyuk;Lee, Young-Hie
    • Proceedings of the KIEE Conference
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    • 2007.11a
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    • pp.114-115
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    • 2007
  • In this study, low temperature sintering property of the $0.6TiTe_3O_8-0.4MgTiO_3$ ceramics with sintering adds were investigated for LTCC application which enable to cofiring with Ag electrode. $TiTe_3O_8$ mixed with $MgTiO_3$ to improve the temperature property. In the X-ray diffraction patterns, the columbite structure of $TiTe_3O_3$ phase and ilmenite structure of $MgTiO_3$ phase were coexisted in all specimens. In the case of $H_3BO_3$ addition, the bulk density and dielectric constant were decreased but quality factor was increased with amount of $H_3BO_3$ additions. The TCRF of the $0.6TiTe_3O_8-0.4MgTiO_3+xwt%H_3BO_3$ ceramics were moved to positive direction. In another case, SnO addition, the bulk density and dielectric constant were increased but Quality factor was decreased with amount of SnO additions. The TCRF of the $0.6TiTe_3O_8-0.4MgTiO_3$+ywt%SnO ceramics were shifted to negative direction. The dielectric constant, quality factor and TCRF of the $0.6TiTe_3O_8-0.4MgTiO_3$ ceramics with $2wt%H_3BO_3$ and 2.5wt%SnO sintered at $830^{\circ}C$ for 1h, were 28.5, 39,570GHz, $+9.34ppm/^{\circ}C$ and 29.86, 35,80000z, $-0.58ppm/^{\circ}C$, respectively.

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Characteristic of Electrical Conductivity in the $\textrm{CuO}-\textrm{Bi}_{2}\textrm{O}_3-\textrm{V}_2\textrm{O}_5$ Glass System with Various Compositions ($\textrm{CuO}-\textrm{Bi}_{2}\textrm{O}_3-\textrm{V}_2\textrm{O}_5$계 글라스에서 조성 변화에 따른 전기 전도도의 특성)

  • Park, S.S.;Jeong, D.J.;Lee, H.;Park, C.Y.;Min, S.K.;Park, H.C.
    • Korean Journal of Materials Research
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    • v.8 no.12
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    • pp.1110-1114
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    • 1998
  • The crystallization behaviors and electrical conductivities of the glasses heat-treated at various times and temperatures in the CuO-Bi$_2$O$_3$-V$_2$O$_{5}$ glass system were investigated. Among glass samples with various compositions, the highest conductivity obtained in the 31CuO-14Bi$_2$O$_3$-55V$_2$O$_{5}$ (mol%) glass sample. The 31CuO-14Bi$_2$O$_3$-55V$_2$O$_{5}$ (mol%) glass sample crystallized by heat treatment at 358$^{\circ}C$ for 8h had 2.67$\times$10$^{-2}$ $\Omega$$^{-1}$ $cm^{-1}$ /, which was much high value as a solid electrolyte. Compared to the glass sample, the heat- treated glass sample was increased in conductivity by an order of 10$^3$-10$^4$due to the formation and growth of BiVO$_4$ and CuVO$_3$crystals.

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Syntheses of Tetradentate Nitrogen-Oxygen(N2O2)) Ligands with Substituents and the Determination of Stability Constants of Their Heavy(II) Metal Complexes (치환기를 가진 질소-산소(N2O2)계 네 자리 리간드의 합성과 중금속(II)이온 착화합물의 안정도상수 결정)

  • Kim, Sun-Deuk;Seol, Jong-Min
    • Journal of Environmental Science International
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    • v.21 no.4
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    • pp.421-435
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    • 2012
  • Novel $N_2O_2$ tetradentate ligands, H-3BPD and H-2BPD were synthesized. Hydrochloric acid salts of Br-3BPD, Cl-3BPD, Br-2BPD and Cl-2BPD having Br and Cl substituents at the $para$ position of the phenol hydroxyl group, were synthesized. The ligands were characterized by C. H. N atomic analysis, $^1H$ NMR, $^{13}C$ NMR, UV-visible, and mass spectra. The proton dissociation constants ($logK_n{^H}$) of the phenol hydroxyl group and secondary amine of the synthesized $N_2O_2$ ligands were shown by four step wise values. The orders of the calculated overall proton dissociation constants ($log{\beta}_p$) were Br-3BPD < Cl-3BPD < H-3BPD in case of 3BPD and Br-2BPD < Cl-2BPD < H-2BPD in case of 2BPD respectively. The order agreed well with that of $para$ Hammett substituent constants(${\delta}_p$). The stability constants($logK_{ML}$) of the complexes between the synthesized ligands and transition metal(II) ions agreed with the order of $log{\beta}_p$ of the ligands. The order of the $logK_{ML}$ value of the each transition metal (II) ion was Co(II) < Ni(II) < Cu(II) > Zn(II) > Cd(II) > Pb(II), which agreed well with that of Iriving-Williams series.

Protective Effects of Chongmyunggongjin-dan on H2O2-induced C6 Glial Cell Death (H2O2로 유발된 C6 신경교세포 사멸에 대한 총명공진단의 보호 효과)

  • Hwang, Gyu-sang;Shin, Yong-jeen
    • The Journal of Internal Korean Medicine
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    • v.41 no.1
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    • pp.44-58
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    • 2020
  • Objectives: This study was conducted to identify the protective effects of Chongmyunggongjin-dan (CMGJD) on Hydrogen peroxide (H2O2)-induced apoptosis mechanisms in C6 glial cells. Method: We used CMGJD after distilled water extraction, filtration, and lyophilization. The ROS scavenging effect was examined by fluorescence microscopy. Expression levels of proteins related to ROS generation were investigated by western blotting. Functional changes in organelles related to Reactive oxygen species (ROS) generation were investigated by immunoblotting and by verifying expression level of relevant enzymes. Results: The CMGJD extract protected the cells against H2O2-induced morphological changes and DNA fragmentation, inhibited the increase of Heme_oxygenase-1(HO-1) and the decrease in catalase, protected against the loss of mitochondrial membrane potential, inhibited disturbances of lysosomal function, and induced an increase in peroxisomes. Conclusion: CMGJD was confirmed to have a protective effect on H2O2-induced C6 glial cell death possibly by blocking the pathways causing damage to subcellular organelles, such as mitochondria, lysosomes, and peroxisomes. We assume that CMGJD will be effective for the prevention and treatment of ischemic stroke in a clinical environment.

Removal of Total Organic Carbon and Micropollutants in Tertiary Treated Sewage by Medium Pressure UV/H2O2 (중압 자외선과 과산화수소 공정을 이용한 하수 3차 처리수중 총유기탄소와 미량오염물질 제거)

  • Lee, Jai-Yeop;Kim, Ilho
    • Journal of Korean Society on Water Environment
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    • v.36 no.4
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    • pp.314-321
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    • 2020
  • This study evaluated the applicability of UV-AOP process using medium-pressure UV lamp and H2O2 to remove TOC and emerging micropollutants in the effluent from a sewage treatment plant. The UV lamp with higher output(1.6~8.0 kW) showed slightly higher amount of power in removing TOC of 1 mg/L(0.09 kWh/mg/L~0.11 kWh/mg/L), however it was found that there was no significant difference for each cases. In addition, under the condition that the H2O2 concentration is sufficient, as the power consumption of the UV lamp increases, the unit TOC removal concentration per unit H2O2 decomposition concentration also increases, resulting in effective removal of TOC. The removal rate of 7 new trace contaminants, such as antibiotics by the UV-AOP tested, was at least 89.4%, and the ability to remove the emerging micro pollutants in the process was very effective. But, it was judged that it could not be excluded that the probablity of transforming to oxidated by-product in the case of a low TOC removal efficiency. Depending on the operating conditions of the UV and H2O2 processes, a higher BOD concentration is found in the treated water than in the influent, and it is necessary to review the UV power and proper injection conditions of H2O2 to maintain the BOD concentration increase below a certain level.

Protective Effect of Ginsenoside Rgl on H2O2-Induced Cell Death by the Decreased Ceramide Level in LLC-PK1 Cells

  • Lee, Youn-Sun;Yoo, Jae-Myung;Shin, Hyun-Woo;Kim, Dong-Hyun;Lee, Yong-Moon;Yun, Yeo-Pyo;Hong, Jin-Tae;Oh, Sei-Kwan;Yoo, Hwan-Soo
    • Journal of Ginseng Research
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    • v.30 no.1
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    • pp.1-7
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    • 2006
  • Ceramide has been involved in celt death and acted as a lipid mediator of stress responses. Elevation of ceramide level was reported to occur in oxidative stress and lead to cell death in many cell types. This study was undertaken to elucidate a protective role of ginsenoside Rgl in cell death induced by oxidative stress. When LLC-PK1 cells were treated with $H_2O_2$ at a concentration of $400{\mu}M$ for 5 hr, cell death was observed and a released LDH activity indicative of cytotoxicity was Increased. $H_2O_2$ exposure to LLC-PK1 cells was shown to elevate the content of total ceramide by approximately 200% compared to control cells. Ceramide level was hypothesized to be a key to a reversal of cell death to survival. Ginsenoside Rgl at the concentrations ranging from 12.5 to $250{\mu}M$ protected LLC-PK1 cells from cell death induced by $H_2O_2\;at\;400{\mu}M$ for 5 hr, and decreased the ceramide level relative to $H_2O_2$. Ginsenoside Rgl inhibited neutral human ceramidase by 71% of controls, while sphingomyelinase was not inhibited. These results suggest that ginsenoside Rgl show the protection against cell death via the modulation of ceramide metabolism, and ceramide may be a promising therapeutic target for human diseases related to cell death.

The Influence of Temperature on the Recovery Reaction of Silver Based on the Pourbaix Diagram (Pourbaix Diagram에 의거한 은(銀)의 회수반응(回收反應)에 미치는 온도영향(溫度影響))

  • Won, Yu-Ra;Kim, Dong-Su
    • Resources Recycling
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    • v.21 no.6
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    • pp.74-81
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    • 2012
  • The Influence of temperature on the recovery reaction of silver in aqueous solution was investigated based on Pourbaix diagram constructed by thermodynamic calculation at different temperatures. It was observed that the stability of water is more strongly affected by pH variation and the stable region of ${Ag^+}_{(aq)}$ is diminished at higher temperature. It was shown that the recovery of $Ag_{(aq)}$ in the forms of $Ag_{(s)}$ and $Ag_2O_{3(s)}$ is more advantageous thermodynamically at lower temperature, however, the recovery of $Ag_{(aq)}$ in the forms of $Ag_2O_{(s)}$$Ag_2O_{2(s)}$ is more advantageous as temperature increases. The rise of temperature is considered to demote the recovery of silver thermodynamically in strong acidic condition ($pH{\leq}2$), but more silver is regarded to be recovered with temperature above pH 2. Finally, The recovery of silver in the elemental state is shown to be more sensitively influenced by temperature variation compared with the recovery of silver in its oxide form.

Protectvie effects of Lonicerae Japonicae Flos against hydrogen peroxidase-induced oxidative stress on Human keratinocyte, HaCaT cells (Hydrogen peroxide로 산화적 스트레스가 유도된 HaCaT keratinocyte에서 금은화의 세포 보호 효과)

  • Seo, Seung-Hee;Choi, Mee-Ok
    • The Korea Journal of Herbology
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    • v.28 no.4
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    • pp.57-62
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    • 2013
  • Objectives : Lonicerae Japonicae Flos (LJF) has been shown anti-oxidant, anti-inflammatory, anti-viral, anti-rheumatoid properties. However, it is still largely unknown whether LJF inhibits skin injury against oxidative stress in human keratinocyte, HaCaT cells. The purpose of this study was to evaluate the protective effects of LJF against hydrogen peroxide($H_2O_2$)-induced oxidative stress in human keratinocytes, HaCaT cells. Methods : To evaluate out the protective effects of LJF on oxidative injury in HaCaT cells, an oxidative stress model of HaCaT cells was established under a suitable concentration (500 ${\mu}M$) hydrogen peroxide. HaCaT keratinocyte cells were pre-treated with LJF (0.1, 0.25 or 0.5 mg/ml), and then stimulated with $H_2O_2$. Then, the cells were harvested to measure the cell viability, DNA damage, and release of reactive oxygen species (ROS). Results : LJF (0.1, 0.25 or 0.5 mg/ml) itself did not show any significant toxicity in HaCaT cells. The treatment of $H_2O_2$ caused the oxidative stress, leading to the cell death, and DNA injury. However, pretreatment with LJF reduced cell death, and DNA injury. The stimulation of $H_2O_2$ on HaCaT cells resulted in excessive release of ROS, which is the main factor of oxidative stress. The excessive release of ROS was inhibited by LJF treatment significantly. Conclusions : These results could suggest that LJF exhibited the protective effects of HaCaT cells against $H_2O_2$-induced oxidative stress by inhibiting ROS release. It could be explained that LJF inhibit skin damages against oxidative stress. Thus, LJF would be useful for the development of drug or cosmetics treating skin troubles.

Research for Deposition of $CeO_2$ Buffer Layer on Coated Conductor by Electron Beam Evaporation (전자빔 증발법에 의한 박막형 고온초전도체의 $CeO_2$ 버퍼층 증착 연구)

  • Lee, J.B.;Park, S.K.;Kim, H.J.;Moon, S.H.;Lee, H.G.;Hong, G.W.
    • Progress in Superconductivity
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    • v.11 no.2
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    • pp.123-127
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    • 2010
  • The properties of buffer layer for thermal and chemical stability in coated conductor is a very important issue. $CeO_2$ has desirable thermal and chemical stability as well as good lattice match. In this study, $CeO_2$ was deposited by electron beam deposition. The MgO(001) single crystal and LMO buffered IBAD substrate(LMO/IBAD-MgO/$Y_2O_3/Al_2O_3$/Hastelloy) were used as substrates, which have $\Delta\phi$ values of ${\sim}8.9^{\circ}$. The epitaxial $CeO_2$ films was deposited with high deposition rate of $12{\sim}16\;{\AA}/sec$. During deposition, the change of oxygen partial pressure(${\rho}O_2$) does not cause change in c-axis texture. In case of $CeO_2$ on MgO single crystal, the substrate temperature was optimized at $750^{\circ}C$ with superior $\Delta\phi$ and $\Delta\omega$ value. Otherwise, In case of LMO buffered IBAD substrate, It was optimized at $650^{\circ}C$ with increasing its deposition thickness of $CeO_2$, which was finally obtained with best $\Delta\phi$ value of $5.5^{\circ}$, $\Delta\omega$ value of $2^{\circ}$ and Ra value of 2.2 nm.