• Title/Summary/Keyword: $HT_{8}$

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Acupuncture Treatment at HT8 Protects Hippocampal Cells in Dentate Gyrus on Kainic Acid-Induced Epilepsy Mice Model (소부혈(少府穴) 자침(刺鍼)이 Kainic Acid로 유도(誘導)된 간질(癎疾) 동물(動物) 모델의 해마(海馬) 치상회(齒狀回)에 미치는 영향(影響))

  • Kim, Seung-Tae;Chung, Joo-Ho;Jeong, Wu-Byung;Kim, Jang-Hyun;Kang, Min-Jung;Hong, Mee-Sook;Park, Hae-Jeong;Kim, Yeon-Jung;Park, Hi-Joon;Lee, Hye-Jeong
    • Korean Journal of Acupuncture
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    • v.24 no.4
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    • pp.99-110
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    • 2007
  • Objectives : Epilepsy is one of the most common serious brain disorders that affect people of all ages, and it is characterized by recurrent unprovoked seizures. We examined whether acupuncture can reduce both the incidence of seizures and hippocampal cell death in dentate gyrus (DG) using a mouse model of kainic acid (KA)-induced epilepsy. Methods : ICR mice ($20{\sim}25$ g) were given acupuncture once a day at acupoint HT8 (sobu) bilaterally during 2 days before KA injection. After an intracerebroventricular injection of 0.1${\mu}g$ of KA, acupuncture treatment was subsequently administered once more (total 3 times), and the degree of seizure was observed for 20 min. Three hours after injection, we confirmed the neural cell death using cresyl violet staining and silver impregnation staining, and determined the expressions of c-Fos and glutamate decarboxylase (GAD)-67 using immunohistochemistry techniques in the DG. Results : KA induced epileptic seizure, neural cell death, increased c-Fos expression and decreased GAD-67 expression in the DG. Acupuncture treatment at HT8 reduced the severity of the epileptic seizure and inhibited neural cell death from KA. In addition, acupuncture normalized the expressions of c-Fos and GAD-67 in the same areas. Conclusions : These results demonstrated that acupuncture treatment at HT8 may reduce the KA-induced epileptic seizure and neural cell death in the DG possibly by normalizing c-Fos expressions and the gamma-aminobutyric acid neurons.

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Morphological Correlation and Chronology of Lower Terrace Formations of Southeastern Coast of Korea (한국 남동해안 저위단구 퇴적층의 지형대비 및 형성시기 고찰)

  • Choi, Weon-Hack;Kim, Ju-Yong
    • Proceedings of the Korean Quaternary Association Conference
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    • 2005.10a
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    • pp.48-54
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    • 2005
  • Terrace morphology is so conspicuous in the south eastern coastal areas. Coastal terraces can be divided into 5 main surfaces, including beach and coastal alluvial plain(AP, $4{\sim}5m$), Low Terrace(LT, 8 $^{\sim}$ 25m), Middle Terrace(MT, 36 $^{\sim}$ 55m), High Terrace(HT, 63 $^{\sim}$ 86m) and upper High Terrace(uHT, above 90m). Among them Lower Terrace Formation is distributed between 8m and 20m in altitude. Both Tephra deposited of LT2 formation and OSL datings of sand layers in LT 2 and LT 3 Formations support the age of the LT 2 formation is MIS 5d or 5e, most probably 5e. The age of LT 3 is interpreted MIS 5a, based on tephra production in organic mud layers and OSL dating of sand deposits just above the beach pebbles of the LT 3. Particularly the transgression, possibly equivalent to the well-known Monastirrian episode in the Mediterranean Sea.

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Effect of the Hexane Extract of Saussurea lappa on the Growth of HT-29 Human Colon Cancer Cells (목향 헥산추출물이 대장암세포인 HT-29 세포의 증식에 미치는 영향)

  • Kim, Eun-Ji;Park, Hee-Sook;Lim, Soon-Sung;Kim, Jong-Sang;Shin, Hyun-Kyung;Yoon, Jung-Han
    • Korean Journal of Food Science and Technology
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    • v.40 no.2
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    • pp.207-214
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    • 2008
  • In Asia Saussurea lappa (SL) has been used as a traditional herbal medicine to treat abdominal pain and tenesmus. Recently, in vitro cell culture studies have shown that SL has anti-ulcer, anti-inflammatory, and anti-tumor properties. To explore its potential chemopreventive and chemotherapeutic effects in colon cancer, we examined whether the hexane extract of SL (HESL) could inhibit the growth of HT-29 human colon cancer cells, and investigated the mechanisms for this effect. The cells were cultured with various concentrations (0-5 ${\mu}g/mL$) of HESL. The results indicated that HESL markedly decreased the numbers of viable HT-29 cells; whereas at the concentration of 5 ${\mu}g/mL$, HESL slightly decreased the viable cell numbers of CCD 1108Sk human skin normal fibroblasts at 72 hr. HESL substantially increased the numbers of cells in the sub G1 phase, and dose-dependently increased apoptotic cell numbers. Western blot analysis of the total cell lysates revealed that HESL increased Bax protein levels, but did not affect Bcl-2 levels. HESL induced the cleavage of poly (ADP-ribose) polymerase and caspases 8, 9, 7, and 3. This study demonstrated that HESL inhibits cell growth and induces apoptosis in HT-29 cells, which may be mediated by its ability to increase Bax levels and activate the caspase pathway. These findings may lead to the development of new therapeutic strategies for colon cancer treatment.

Effects of Pre-drying Process on the Quality of 'Fuyu' Persimmons (예건 처리 온도에 따른 단감(부유)의 품질 변화 조사)

  • Park, Sung Jin;Cho, Kwang Sik;Kim, Jun Tae
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.21 no.2
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    • pp.79-84
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    • 2015
  • Pre-drying of 'Fuyu' persimmon was performed right after harvesting from a farm. Pre-drying conditions were varied with room temperature (RT) for 1 day to 7 days, low temperature (LT, at $20{\sim}30^{\circ}C$) for 1 day to 4 days, high temperature (HT, at $30{\sim}40^{\circ}C$) for 3 h to 12 h, and ultra-high temperature (UT, at $50{\sim}60^{\circ}C$) for 30 min to 120 min. Weight loss of pre-dried persimmon was increased from 1.62% up to 2.96% with increased pre-drying temperature and time. Pre-drying at RT resulted more significant weight loss of persimmon compared to that of pre-drying at HT. Minimum firmness loss of persimmon stored at $0^{\circ}C$ for 100 days was obtained at the condition of HT for 6 h. Rate of peel blackening was decreased from 31.5% to 16.4% and 10.9% by pre-drying at HT for 6 h and 9 h, respectively.

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Extraction and Composition of Bound Lipids in Naked Barley (쌀보리 결합지질의 추출과 그 조성에 관한 연구)

  • Kim, Hae-Gyoung;Kim, Bok-Nam;Choi, Hong-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.18 no.1
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    • pp.109-114
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    • 1989
  • Bound lipids(BL) of naked barley(Hordeum vulgare L.) were extracted by different methods and the composition of BL was determined by the procedures of column chromatography, thin layer chromatography and gas chromatography. For the extraction, after free lipids were removed from barley flour by petroleum ether(PE) extraction and then BL were extracted from PE treated flour by the solvent systems of water-saturated butanol(WSB) at $25^{\circ}C$(WSB-LT) and at $95^{\circ}C$ (WSB-HT). BL were extracted by WSB-HT with higher extraction yield as 1.5% as dry basis of flour. The contents of neutral lipids(NL), glycolipids(GL) and phospholipid(PL) in BL were $20.7{\sim}35.5%$, $28.7{\sim}32.4%$, $32.1{\sim}50.6%$, respectively with particularly higher content of PL in WSB-HT as 50.6%. Digalactosyl-diglycerides $(40.2{\sim}44.8%)$, monogalactosyl-diglycerides $(20.3{\sim}31.1%)$, sterly glycerides$(11.2{\sim}15.2%)$ and cereb rosides$(11.6{\sim}12.9%)$ were observed in GL. Of the PL in BL, lysophosphatidyl choline, phosphatidyl choline and phosphatidyl serine, and phosphatidyl ethanolamine were the major components. The predominent fatty acids of NL, GL and PL were linoleic and palmitic acids, however, no significant difference was observed in the composition of fatty acids between two extraction methods.

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Anticancer Effect of COX-2 Inhibitor DuP-697 Alone and in Combination with Tyrosine Kinase Inhibitor (E7080) on Colon Cancer Cell Lines

  • Altun, Ahmet;Turgut, Nergiz Hacer;Kaya, Tijen Temiz
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.7
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    • pp.3113-3121
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    • 2014
  • Colorectal cancer remains one of the most common types of cancer and a leading cause of cancer death worldwide. In this study, we aimed to investigate effects of DuP-697, an irreversible selective inhibitor of COX-2 on colorectal cancer cells alone and in combination with a promising new multi-targeted kinase inhibitor E7080. The HT29 colorectal cancer cell line was used. Real time cell analysis (xCELLigence system) was conducted to determine effects on colorectal cell proliferation, angiogenesis was assessed with a chorioallantoic membrane model and apoptosis was determined with annexin V staining. We found that DuP-697 alone exerted antiproliferative, antiangiogenic and apoptotic effects on HT29 colorectal cancer cells. For the antiproliferative effect the half maximum inhibition concentration ($IC_{50}$) was $4.28{\times}10^{-8}mol/L$. Antiangiogenic scores were 1.2, 0.8 and 0.5 for 100, 10 and 1 nmol/L DuP-697 concentrations, respectively. We detected apoptosis in 52% of HT29 colorectal cancer cells after administration of 100 nmol/L DuP-697. Also in combination with the thyrosine kinase inhibitor E7080 strong antiproliferative, antiangiogenic and apoptotic effects on HT29 colorectal cancer cells were observed. This study indicates that DuP-697 may be a promising agent in the treatment of colorectal cancer. Additionally the increased effects observed in the combination with thyrosine kinase inhibitor give the possibility to use lower doses of DuP-697 and E7080 which can avoid and/or minimize side effects.

Antitumor Effects of Hyperthermic CO2 Pneumoperitoneum on Human Gastric Cancer Cells

  • Zhou, Hou-Min;Feng, Bo;Zhao, Hong-Chao;Zheng, Min-Hua
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.1
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    • pp.117-122
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    • 2012
  • Aim: To elucidate the effects of hyperthermic $CO_2$ pneumoperitoneum on human gastric AGS cells. Methods: Based on a newly devised in vitro study model, we evaluated the anti-cancer effects of HT-$CO_2$ ($42-44^{\circ}C$ for 2-4h) on human gastric cancer cells, and also the corresponding mechanisms. Results: HT-$CO_2$ ($42-44^{\circ}C$ for 2-4h) severely inhibited cell proliferation as assessed by Cell Counting Kit-8 assay, while inducing apoptosis in a temperature- and time-dependent manner demonstrated by annexin-V/PI flow cytometry and morphological analysis (Hoechst/PI fluorescence). In addition, it was found that HT-$CO_2$ ($42-44^{\circ}C$ for 2-4h) promoted the up-regulation of Bax by western blotting. Significantly, it could also suppress gastric cancer cell invasion and metastasis by in vitro invasion and motility assay. Conclusion: In conclusion, HT-$CO_2$ had an efficacious cytotoxic effect on gastric cancer cells through Bax-induced mitochondrial apoptotic signaling. Our studies indicate that it may serve as a potential therapy for peritoneal carcinomatosis of gastric cancer. Further investigations in vivo using animal models are now urgently needed.

Laccase Fermentation of Clove Extract Increases Content of Dehydrodieugenol, Which Has Neuroprotective Activity against Glutamate Toxicity in HT22 Cells

  • Lee, Han-Saem;Yang, Eun-Ju;Lee, Taeho;Song, Kyung-Sik
    • Journal of Microbiology and Biotechnology
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    • v.28 no.2
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    • pp.246-254
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    • 2018
  • Enzyme fermentation is a type of food processing technique generally used to improve the biological activities of food and herbal medicines. In this study, a Syzygii Flos (clove) extract was fermented using laccase derived from Trametes versicolor (LTV). The fermented clove extract showed greater neuroprotective effects against glutamate toxicity on HT22 than the non-fermented extract did. HPLC analysis revealed that the eugenol (1) and dehydrodieugenol (2) contents had decreased and increased, respectively, after fermentation. The content of 2 peaked at 1 h after fermentation to $103.50{\pm}8.20mg/g_{ex}$ (not detected at zero time), while that of 1 decreased to $79.54{\pm}4.77mg/g_{ex}$ ($185.41{\pm}10.16mg/g_{ex}$ at zero time). Compound 2 demonstrated promising HT22 neuroprotective properties with inhibition of $Ca^{2+}$ influx, the overproduction of intracellular reactive oxygen species, and lipid peroxidation. In addition, LTV showed the best fermentation efficacy compared with laccases derived from Pleurotus ostreatus and Rhus vernicifera.

Effect of Active layer (P3HT:PCBM) Thickness on the Performance of Bulk Heterojunction Solar Cells (Active layer (P3HT:PCBM) 두께에 따른 유기물 태양전지의 제작 및 특성 분석)

  • Baek, Woon-Hyuk;Kim, Jung-Min;Yoon, Tae-Sik;Lee, Hyun Ho;Kim, Yong-Sang
    • Proceedings of the KIEE Conference
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    • 2008.07a
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    • pp.1154-1155
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    • 2008
  • 본 연구에서는 전자 주개 물질(electron donor)인 regioregular poly(3-hexylthiophene)(P3HT)와 전자 받개 물질(electron acceptor)인 phenyl-$C_{61}$-butyric acid methyl ester (PCBM)을 혼합한 복합 박막 구조(Bulk Heterojunction)를 이용하여 태양전지를 제작하고 광활성층(Active layer)의 두께를 변화시키면서 광학적 특성 및 전기적 특성에 대해 분석하였다. 광활성층의 두께가 두꺼워 질수록 광흡수율이 높기 때문에 태양전지의 효율이 증가하여 200nm정도의 두께에서 가장 좋은 특성을 보였으며, 그 이상의 두께에서는 광흡수율이 높더라도 직렬저항(Series resistance)의 증가로 개방 회로 전압이 감소하는 것을 볼 수 있었으며, 최적화된 광활성층의 두께(190nm)에서 개방 회로 전압($V_{oc}$)은 0.6V, 단락 회로 전류($J_{sc}$)는 8.29mA, Fill factor(FF)는 0.59, 전력변환효율($\eta$)은 2.94%였다.

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