• 한국미생물·생명공학회지
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• 제28권4호
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• pp.209-213
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• 2000

The cultured mycelia of Ganoderma lucidum were extracted and the extract was separated into six fractions by organic solvent fractionation. The antihepatotoxic activity of all the fractions was evaluated by measuring activities of glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT). Among the fractions tested, the high-polarity fractions such as aqueous and n-butanol fractions significantly reduced activities of GPT and GOT in CCl4- and galactosamine-intoxicated rat primary hepatocytes. When intracellular synthetic activities were measured by pulsing the rate primary cultured hepatocytes with [3H]-uridine and [3H]-leucine, activities of DNA, RNA and protein. When direct toxicities of the fractions were measured against human hepatoma(SK-Hep-1), the non-polarity fractions such as n-butanol and ethyl acetate fractions showed potent direct cytotoxicities even at the concentration of 1 $\mu\textrm{g}$/ml. These data showed that Ganoderma lucidum has hepatoprotective and hepatotoxic recovery principles in its mycelia.

• Toxicological Research
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• 제5권2호
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• pp.135-149
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• 1989

The effect of Ganoderma lucidum (Polyporaceae) extract on initiation and promotion of liver preneoplastic lesions in chemical carcinogenesis were examined in male rats. Male Fisher 344 rats, 8 weeks old, were injected i.p. with diethylnitro-samine(DENA` 200 mg/kg) as an initiator and were fed on diet containing 0.02%-acetaminofluorene (2-AAF) as a promoter for 2 weeks (group 1, 2` 2-4 week) and were fed on basal diet (group 3 and 4). The rats in test groups(group 1 and 3) were fed on diet containing 0.1% of Ganoderma lucidum extract for 6 weeks (2-8 week).

• 농업과학연구
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• 제22권1호
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• pp.54-61
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• 1995

영지 extract가 유당발효성 효모인 Kluyveromyces fragilis KCCM 35458의 발효에 미치는 영향을 조사하기 위하여 탈지유 배양액에 영지 1호의 extract를 0%, 0.1%, 0.5% 및 1.0%의 농도별로 첨가하고 $30^{\circ}C$에서 72시간을 정치배양하면서 조사한 결과는 다음과 같다. 1. 영지 extract를 1.0% 첨가한 시험구(試驗區)는 대조구(對照區)에 비하여 시간이 경과함에 따라 pH에서 빠른 감소를 보였으며 적정산도에서는 빠른 증가를 보였다. 2. $CO_2$ 발생량(發生量)은 영지 extract를 첨가한 전(全) 시험구(試驗區)에서 높았으며 1.0% 첨가구의 효과가 월등하였다. 3. Alcohol 생성량(生成量)은 72시간 배양함에 따라 영지 extract 첨가구(添加區)에서 높게 나타났으며 1.0% 첨가구(添加區)에서 가장 높은 alcohol 생산성을 보였고 발효시간을 단축시켰다. 4. 효모수는 영지 extract 0.5%, 1.0%첨가구에서 대조구에 비하여 현저한 증식효과를 나타내었다. 5. 탈지유 배양액에 있어서의 Kluyveromyces fragilis의 유당발효 상태는 배양시간 경과에 따라 영지 extract 첨가구가 대조구에 비하여 발효가 촉진되었으며 1.0% 영지 extract 첨가구에서 효과적이었다.

• 산업기술연구
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• 제20권A호
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• pp.45-50
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• 2000

A submerged cultivation of Ganoderma lucidum was carried out in an air-lift fermenter system, and the effects of different pH processes on extracellular branched ${\beta}$-1,3-glucan(EPS) production and mycelial growth(MDW) were investigated. The controlled pH process improved the production of branched ${\beta}$-1,3-glucan and biomass in comparison to the uncontrolled pH process. However, the maximum production of branched ${\beta}$-1,3-glucan were obtained by the bi-staged pH process. From these results, we confirmed that the bi-staged pH process was the most effective for improving the production of branched ${\beta}$-1,3-glucan from submerged culture of G. lucidum.

• 한국균학회지
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• 제27권1호통권88호
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• pp.39-43
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• 1999

불로초의 계통 분류학적 조사를 위해 불로초속 중 8종(Ganoderma lucidum, G. tsugae, G. pfeifferi, G. resinaceum, G. australe-applanatum, G. oregonense, G. neo-japonicum, G. applanatum) 12균주와 out-group 균주로는 Inonotus xeranticus의 rDNA ITS II를 PCR로 증폭하여 염기서열을 비교 조사하였다. 불로초 속 중 ITS II 영역의 길이는 $247{\sim}257\;bp$로 분포하였고 종간 상동성은$70{\sim}100%$ 로 조사되었다. 계통도를 분석한 결과는 5개의 군(cluster)을 형성하였고 G. tsugae는 각각의 G. lucidum과 하나의 clade를 이루어 두 종은 서로 매우 밀접하게 진화된 것으로 사료되었다. 본 조사에서는 국내에서 재배되고 있는 불로초(G. lucidum)는 그 발생지 및 국외 ITS염기서얼 자료와 비교 및 분석할 때 G. tsugae로 하는 것이 타당할 것으로 사료되었다.

• 한국식품조리과학회지
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• 제21권6호통권90호
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• pp.759-768
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• 2005

This study was performed to determine the antioxidative effect of the hexane, dichloromethane, ethylacetate, butanol and water fractions of Ganoderma lucidum extracts on the inhibition of malondialdehyde(MDA) and bovine serum albumin(BSA) conjugation reaction, the inhibition of lipid peroxidation and the scavenging effect on 1,1-diphenyl-2-picryl-hydrazyl(DPPH) radical, the antimutagenic capacity as measured by the Ames test and the inhibitory effect on cancer cell. Ganoderma lucidum is believed to have possible antioxidative capacities, although the results have varied according to the assay method. The most effective antioxidative capacity was inhibition of lipid peroxidation. Among the five fractions, water fraction showed strong inhibition rates on MDA & BSA conjugation reaction, and ethylacetate fractions showed the most effective inhibition rate on lipid peroxidation and scavenging effect on DPPH radical. The indirect and direct antimutagenic effects of ethanol extracts of Ganoderma lucidum were examined by Ames test using Salmonella typhimurium TA98 and TA100. Among the samples, the water fraction did not have any antimutagenic effect. The inhibition rates on mutagenicity in the presence of 2.5 mg/plate were nearly $100\%$ for Salmonella typhimurium TA98 and TA100 except the hexane fraction of the direct mutagenicity mediated by 2-Nitrofluorene in Salmonella typimurium TA98($64.69\%$). Under the 2.5 mg/plate concentration, the inhibitory effects of hexane and dichloromethane fraction were superior to that of the other fractions on the direct mutagenicity for Salmonella typhimurium TA100 and indirect mutagenicity for Salmonella typhimurium TA98 and TA100. The inhibitory effect of Ganoderma lucidum extracts on cell proliferation in HeLa and MCF-7 was investigated by U test. The dichloromethane fraction showed highly antiproliferative effect in HeLa and MCF-7($IC_{50}$: 0.122 mg/mL, 0.272 mg/mL, respectively) cells while the water faction had a weak inhibitory effect($IC_{50}$: 0.691 mg/mL, 10.919 mg/mL respectively). These results suggest that Ganoderma lucidum may have antioxidative, antimutagenic and anticancer capacities and may be a candidate of the prevention and dietetic treatment of chronic diseases and the development of antioxidative, antimutagenic and anticancer functional food.

• 한국식품영양학회지
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• 제7권1호
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• pp.45-50
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• 1994

This study was carried out to Investigate the effects of Panax ginseng, Ganoderma lucidum extract and crude polysaccharide of G. lucidum on the growth of lactic acid bacteria. p. ginseng extract contained 60.7% carbohydrate and 27.5% protein, whereas G. lucidum contained 35.9% carbohydrate and 46.3% protein. The total sugar and protein content of crude polysaccharide of G. lucidum were 47.2% and 15.2%, respectively. Two amino acids(hg, Trp) were detected in p. ginseng extract and 11 amino acids (hg, Trp, Ua, Lys, Ser, etc.) in C. lucidum extract. By the addition of p. ginseng, 5. lucidum extract and crude polysaccharide, the cia. p. ginseng was more effective on the growth of 1. casei an: G. lucidum was more effective on that of S. thermophilus. The effect of free amino acids on the growth of tactic acid bacteria was also examined. Arginine and lysine stimulated the growth of L. casei, whereas Lysine, serine, arginine, and glutamic acid stimulated the growth of 5. thermophilus.

• Biomolecules & Therapeutics
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• 제11권2호
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• pp.139-144
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• 2003

Ganoderma lucidum is commonly known as medically potent mushroom, which has been widely used in China and other oriental countries for the treatment of various diseases, including cancer. In this report, we investigated the anti-oxidant and protective effect of Ganodema lucidum extract (GLE) against the DNA damage induced by free radical and U.V. In the assay of cell growth inhibition, the inhibitory cell growth rate induced by hydroxyl radical was dose-dependently decreased by GLE. This results support that GLE has a detoxifying activity against cytotoxicity of hydroxyl radical in E. coli cell. GLE also protected ColE1 plasmid DNA damage in the concentration of 200$\mu\textrm{g}$ per reaction on the DNA fragmentation assay. The nuclear tailing by hydrogen peroxide in single cell gel electrophoresis(SCGE) was decreased by GLE in the concentration of 50$\mu\textrm{g}$/ml. These data indicate that Ganoderma lucidum has an anti-oxidative activity to hydrogen peroxide. The mutation rate after irradiation of U.V. was reduced by 50$\mu\textrm{g}$/ml GLE and total number of Rif (Rifampicin) resistant mutants was decreased in a concentration dependent manner when added the GLE exogenously in a culture media. According to the results, it is likely that GLE has not only an anti-oxidative activity to hydroxyl radical but also an anti-mutagenic activity to U.V. mutagenesis.

• 한국미생물·생명공학회지
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• 제14권4호
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• pp.279-283
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• 1986

홍삼제품 제조 과정중의 부산물인 홍삼박의 활용 가능성을 조사하기 위하여 여러 가지 담자균의 배양에 홍삼박 추출물을 첨가하여 배양한 후 균체 증식량, 단백질 및 amino acid함량 등을 조사하였으며 그 결과를 보고하는 바이다. 수집 된 담자균 중 G. lucidum과 P. ostreatus가 액체배양에서 균체증식이 양호한 균주로 선발되었으며, 홍삼박 추출물을 0.2％ 첨가하였을 때 균체증식량이 가장 많았으며 단백질 함량은 0.5％ 첨가하였을 때 l-3％가 증가하였다. Amino acid함량은 인삼박 추출물을 0.2％ 첨가하였을 때는 거의 변화가 없었으나 0.5％ 첨가하였을 때에는 G. lucidum, P. ostreatus 모두 상당히 증가하였으며 그 증가폭 중 cysteine이 가장 컸다.

• Mycobiology
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• 제40권1호
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• pp.71-75
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• 2012

In the present study, a phylogenetic analysis was undertaken based on the internal transcribed spacer (ITS) rDNA and partial ${\beta}$-tubulin gene sequence of the $Ganoderma$ species. The size of the ITS rDNA regions from different $Ganoderma$ species varied from 625 to 673 bp, and those of the partial ${\beta}$-tubulin gene sequence were 419 bp. Based on the results, a phylogenetic tree was prepared which revealed that Korean $Ganoderma$ $lucidum$ strains belong in a single group along with a $G.$ $lucidum$ strain from Bangladesh.