• Asian-Australasian Journal of Animal Sciences
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• v.22 no.2
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• pp.155-161
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• 2009

Mammal microsomal glutathione transferase 1 (MGST1) can conjugate many toxic or carcinogenic substances and depress oxidative stress. In this study, Chicken MGST1 and its variants were cloned for the first time and were composed of 956 or 944 nucleotides. The 12 nt deletion in the exon 2 did not alter the GT-AG rule and the ORFs for the two MGST1 variants were the same, which both comprised 465 nucletides and encoded a peptide with 155 amino acids. It was found that the two different splice variants identified using RT-PCR expressed in all three organs investigated of Dwarf Brown Chicken, namely liver, spleen and shell gland. Moreover, the expression level of MGST1 mRNA in the liver of Dwarf Brown chickens was the highest (p<0.01), and there were no significant differences between the spleen and the shell gland. These results provide a base for studying the biological function of Chicken MGST1.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.5
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• pp.3085-3091
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• 2013

Associations between ABCB1 and XPC genetic polymorphisms and risk of developing colorectal cancer (CRC) as well as clinical outcomes in CRCs with chemotherapy were investigated. A case-control study was performed on the ABCB1 C3435T, G2677T/A and XPC Lys939Gln polymorphisms in 428 CRC cases and 450 hospitalbased, age and sex frequency-matched controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays. We observed that the ABCB1 3435CT or CC+CT variants were significantly linked with increasing risk of developing CRC (adjusted OR (95% CI): 1.814 (1.237-2.660), P=0.0022; adjusted OR (95% CI): 1.605 (1.117-2.306), P=0.0102, respectively). Moreover, the distribution frequency of XPC AC genotype or AC+CC genotypes also showed a tendency towards increasing the suscepbility for CRC (P=0.0759 and P=0.0903, respectively). Kaplan-Meier curves showed that the ABCB1 C3435T variant was associated with a tendency toward longer progression-free survival (PFS) (n=343, Log-rank test: P=0.063), and the G2677T/A variant genotypes (GT+TT+GA+AA) with a tendency for longer OS in postoperative oxaliplatin-based patients (n=343, Log-rank test: P=0.082). However, no correlation of the XPC Lys939Gln polymorphism was found with PFS and OS in patients with postoperative oxaliplatin-based chemotherapy (n=343). Our study indicated that ABCB1 polymorphisms might be candidate pharmacogenomic factors for the prediction of CRC susceptibility, but not for prognosis with oxaliplatin chemosensitivity in CRC patients.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.2
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• pp.747-752
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• 2013

Oncostatin M (OSM) is a multifunctional cellular regulator acting on a wide variety of cells, which has potential roles in the regulation of gene activation, cell survival, proliferation and differentiation. Previous studies have shown that OSM can induce morphological and/or functional differentiation and maturation of many tumor cells. However, the action of OSM on the induction of differentiation of human hepatocellular carcinoma (HCC) has not been reported. Here, we investigated the effects of different concentrations of OSM on human HCC cell line SMMC-7721 growth, proliferation, cell cycling, apoptosis and differentiation in vitro. Cell growth was determined via MTT assay, proliferation by cell cycle analysis, apoptosis by flow cytometry, morphology by transmission electronic microscopy, and cell function by detection of biochemical markers. Our results demonstrated that OSM strongly inhibited the growth of SMMC-7721 cells in a dose-dependent manner, associated with decreased clonogenicity. Cell cycle analysis revealed a decreased proportion of cells in S phase, with arrest at G0/G1. The apotosis rate was increased after OSM treatment compared to the control. These changes were associated with striking changes in cellular morphology, toward a more mature hepatic phenotype, accompanied by significant reduction of the expression of AFP and specific activity of ${\gamma}$-GT, with remarkable increase in secretion of albumin and ALP activity. Taken together, our findings indicate that OSM could induce the differentiation and reduce cell viability of SMMC-7721 cells, suggesting that differentiation therapy with OSM offers the opportunity for therapeutic intervention in HCC.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.14 no.4
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• pp.260-264
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• 1981

The purpose of the present study is to measure the sound spectrum of the underwater noise generated by a stern trawler M/S Saebada (2,275 GT, 3600 ps) in the various operational conditions. Underwater noises were recorded by a hydrophone (B & K 8100) and analyses were made rising a digital frequency analyzer (B & K 2131) and level recorder (B & K 2370). The predominant frequency range was 100-500 Hz, and maximum sound pressure level was 121 dB(re. $1{\mu}Pa$). Underwater noise level increased with the increased speed of the vessel. Sound pressure level measured in the course of astern cruising was higher than that measured in the course of ahead cruising and also the noise spectrums were different in these two cases. At the time of cruising the underwater noise was higher than 10 dB compared to those values measured at the time at rest with only engine operation. The underwater noise of the vessel was mainly due to the main engine revolution of the propeller and the vibration of hull.

• Journal of Korea Game Society
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• v.11 no.2
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• pp.151-159
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• 2011

For the development of a curriculum of the serious game for the elderly in preparation of senior society, we have surveyed students of both college of humanity and college of engineering for their perception of importance and necessity on the subjects prepared by this team. We analyzed their common points and different points, and developed a new interdeciplinary curriculum for students in both college. The curriculum has been opened in the last semester successfully.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.1
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• pp.63.1-63.1
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• 2013

To improve the forecast capability of geomagnetic storms, we consider the real time solar and near Earth conditions together, since the characteristics of CMEs can be modified during their transit from the Sun to the Earth, and the geomagnetic storms may be directly affected by not only solar events but also near Earth interplanetary conditions. Using 55 CME-Dst pairs associated with M- and X-class solar flares, which have clearly identifiable source regions during 1997 to 2003, we confirm that the peak values of negative magnetic field Bz and duskward electric field Ey prior to Dst minimum are strongly related with Dst index. We suggest the solar wind criteria (Bz<-5 nT or Ey>3 mV/m for t>2 hr) for moderate storm less than -50 nT by modifying the criteria for intense storms less than -100 nT proposed by Gonzalez and Tsurutani (GT, 1987). As the results, 90% (28/31) of the storms are correctly forecasted by our criteria. For 15 exceptional events that are incorrectly forecasted by only CME parameters, 12 cases (80%) can be properly forecasted by solar wind criteria. When we applying CME and solar wind conditions together, all geomagnetic storms (Dst<-50 nT) are correctly forecasted. Our results show that, the storm forecast capability of the 2~3 days advanced warning based on CME parameters can be improved by combining with the urgent warning based on the near Earth solar wind condition.

• Journal of Korean Biological Nursing Science
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• v.17 no.2
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• pp.104-113
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• 2015

Purpose: This study aimed to analyze physical activity as measured by the International Physical Activity Questionnaire (IPAQ) and an actigraph in breast cancer survivors, as well as to identify their intention to participate in a physical activity program. Methods: Breast cancer patients who had been diagnosed for more than six months (N=135) at a university hospital participated from June 2012 to May 2013. Physical activity was measured using the Korean version of the IPAQ-Short Form and Actigraph GT3X plus an accelerator for seven consecutive days. Data analyses were conducted using the SPSS WIN 19.0 program. Results: Mean total physical activity was 2298.21 metabolic equivalent task (MET)-min/week as assessed by IPAQ and 150,140.57 counts/day as measured by an actigraph. There were statistically significant correlations between moderate physical activity from IPAQ and light intensity of physical activity from the actigraph (r=.735, p<.001), vigorous physical activity from IPAQ and vigorous intensity of physical activity from the actigraph (r=.871, p<.001), total physical activity from IPAQ and light intensity of physical activity from the actigraph (r=.825, p<.001), respectively. Most (80.7%) cancer survivors reported a positive attitude toward physical activity and 57.8% expressed a willingness to participate in a physical activity program. More than half (60%) of the subjects preferred walking, 80.6% preferred more than 30 minutes of exercise, and 57.1% wanted to engage in physical activity three times a week and preferred home-based activities. Perceived barriers included fatigue, lack of strength and pain. Conclusion: It is necessary to consider intensity, personal preferences, and patient-perceived barriers when developing physical activity programs for breast cancer survivors.

• Animal cells and systems
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• v.14 no.2
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• pp.83-89
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• 2010

Gangliosides are a ubiquitous component of the membranes of mammalian cells that have been suggested to play important roles in various cell functions such as cell-cell interaction, adhesion, cell differentiation, growth control and signaling. However, the role that gangliosides play in the immune rejection response in xenotransplantation is not yet clearly understood. In this study, differential expression patterns of gangliosides in HEK293 (human embryonic kidney cells), PK15 (porcine kidney cells), NIH-kd (NIH-mini pig kidney cells, primary cultured) and the cortex, medulla and calyx of the NIH-mini pig kidney were investigated by high-performance thin-layer chromatography (HPTLC). The results revealed that HEK293, PK15 and NIH-kd contained GM3, GM2 and GD3 as major gangliosides. Moreover, GM3, which are the gangliosides of NIH-kd, were expressed at higher levels than HEK293 and PK15. Especially, GT1b were expressed in HEK293 and NIH-kd but not in PK15. Finally, GM1 and GD1a were expressed in NIH-kd, but not in HEK293 or PK15. These results suggest that differential expression patterns of gangliosides from HEK293, PK15 and NIH-kd are related to the immune rejection response in xenotransplantation.

• Journal of Adhesion and Interface
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• v.13 no.2
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• pp.73-84
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• 2012

The transfer mechanism of optical information at the interface of air and glass (the air-glass IF) has been investigated by thoroughly fulfilling the theoretical and experimental analyses regarding the FTIR (Frustrated Total Internal Reflection) and moisture effects on the fingerprint onto a glass cup with water. As for the fingerprint onto a glass cup with water its image was observed to be very vivid, which turned out to be due to the difference between the two light intensities reflected on the air-glass IF and the wet fingerprint ridge by manipulating the optical theories such as Fresnel relation, Snell's law, FTIR, GT (general transmission) and so on. In addition, the experimental inspection for FTIR and moisture effects on the fingerprint image also evidenced the fact that the vivid fingerprint image originated from the moisture effect rather than the FTIR phenomenon.

• Journal of Energy Engineering
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• v.25 no.2
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• pp.29-36
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• 2016

In this study, diesel/natural gas dual-fuel engine was studied numerically using DoE method. The engine is CI engine for power generation and modelled by 1-D simulation GT-power. The combustion and emission characteristics were analyzed as a function of IVO, IVC and the ratio of natural gas to total fuel enegy. As the proportion of natural gas increases, the BSFC(Brake specific fuel consumption) is increased and BSNOx(Brake specific NOx) is decreased. If specific valve timing to improve the BSFC is applied to the engine, the BSFC is decreased by 1% and simultaneously BSNOx is decreased by 36%.