• Journal of Microbiology and Biotechnology
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• 제26권1호
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• pp.120-129
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• 2016

An industrial complex in Wonju, contaminated with trichloroethene (TCE), was one of the most problematic sites in Korea. Despite repeated remedial trials for decades, chlorinated ethenes remained as sources of down-gradient groundwater contamination. Recent efforts were being made to remove the contaminants of the area, but knowledge of the indigenous microbial communities and their dechlorination abilities were unknown. Thus, the objectives of the present study were (i) to evaluate the dechlorination abilities of indigenous microbes at the contaminated site, (ii) to characterize which microbes and reductive dehalogenase genes were responsible for the dechlorination reactions, and (iii) to develop a PCE-to-ethene dechlorinating microbial consortium. An enrichment culture that dechlorinates PCE to ethene was obtained from Wonju stream, nearby a trichloroethene (TCE)-contaminated industrial complex. The community profiling revealed that known organohalide-respiring microbes, such as Geobacter, Desulfuromonas, and Dehalococcoides grew during the incubation with chlorinated ethenes. Although Chloroflexi populations (i.e., Longilinea and Bellilinea) were the most enriched in the sediment microcosms, those were not found in the transfer cultures. Based upon the results from pyrosequencing of 16S rRNA gene amplicons and qPCR using TaqMan chemistry, close relatives of Dehalococcoides mccartyi strains FL2 and GT seemed to be dominant and responsible for the complete detoxification of chlorinated ethenes in the transfer cultures. This study also demonstrated that the contaminated site harbors indigenous microbes that can convert PCE to ethene, and the developed consortium can be an important resource for future bioremediation efforts.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제10권6호
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• pp.2709-2729
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• 2016

Representation based classification, kernel method and sparse representation have received much attention in the field of face recognition. In this paper, we proposed an improved kernel principal component analysis method based on sparse representation to improve the accuracy and robustness for face recognition. First, the distances between the test sample and all training samples in kernel space are estimated based on collaborative representation. Second, S training samples with the smallest distances are selected, and Kernel Principal Component Analysis (KPCA) is used to extract the features that are exploited for classification. The proposed method implements the sparse representation under ℓ₂ regularization and performs feature extraction twice to improve the robustness. Also, we investigate the relationship between the accuracy and the sparseness coefficient, the relationship between the accuracy and the dimensionality respectively. The comparative experiments are conducted on the ORL, the GT and the UMIST face database. The experimental results show that the proposed method is more effective and robust than several state-of-the-art methods including Sparse Representation based Classification (SRC), Collaborative Representation based Classification (CRC), KCRC and Two Phase Test samples Sparse Representation (TPTSR).

• Biomedical Science Letters
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• 제10권2호
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• pp.153-161
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• 2004

Antibiotic resistance is often associated with the production of inner membrane proteins (for example, AcrAB/TolC efflux pump) that are capable to extrude antibiotics, detergents, dyes and organic solvents. In order to evaluate the unknown MarB function of Escherichia coli, especially focused on the function of OmpF porin, several mutants were construted by T4GT7 transduction. MarA plays a major roles in mar (multiple antibiotic resistance) phenotype with AcrAB/TolC efflux pump in E. coli K-12. Futhermore, MarA decreases OmpF porin expression via micF antisense RNA. Expression of acrAB is increased in strains containing mutation in marR, and in those carrying multicopy plasmid expressing marA. MarB protein of E. coli K-12 showed its activity at OmpF porin & TolC protein as target molecule. Some paper reported MarB positively regulates OmpF function. MarA shows mar phenotype, and MarB along with MarA show decreased MIC through OmpF function. By this experiment, MarB could decrease MIC through the OmpF porin & TolC protein as target.

• Biomedical Science Letters
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• 제14권2호
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• pp.119-122
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• 2008

The baculovirus/insect cell expression system is of great value in the study of structure-function relationships in mammalian glucose-transport proteins by site-directed mutagenesis and for the large-scale production of these proteins for mechanistic and biochemical studies. In order to exploit this, the effects of substitution at the highly conserved residue glutamine 282 of the human erythrocyte-type glucose transporter have been examined by in vitro site-directed mutagenesis. The modified human transport protein has been expressed in Spodoptera frugiperda 21 cells by using the recombinant baculovirus AcNPV-GTL. To assess the functional integrity of the expressed transporter, measurements of the transport inhibitor cytochalasin B binding were performed, involving the membranes prepared from 4 days post infection with no virus, with wild-type virus or AcNPV-GTL virus. Data obtained showed that there was little or no D-glucose-inhibitable binding in cells infected with the wild type or no virus. Only the recombinant virus infected cells exhibited specific binding, which is inhibitable by D- but not by L-glucose. However, there was a notable reduction in the affinity for the potent inhibitor cytochalasin B when binding measurements of AcNPV-GTL were compared with those of AcNPV-GT, which has no substitution. It is thus suggested that although the modified and unmodified human transporters differed slightly in their affinity for cytochalasin B, the glutamine substitution did not interfere the heterologous expression of the human transporter in the insect cells.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• 제22권4호
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• pp.41-48
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• 1986

To investigate the midwater trawl gear available for the Korean near sea trawlers, the authors carried out a field experiment on the attitude and the opening efficiency of the otter board with the Pusan 404 (160GT, 750ps), a training ship of National Fisheries University of Pusan. The experimental trawl gear was designed to be operable by the ship and the otter board was made of single iron plate with 12% camber ratio. The special-prepared potentiometric angle detector was used for determining the attitude, and the 50 KHz fish finder for the opening between the otter boards. The results obtained can be summarized as follows: 1. The angle of attack varied by moving the towing point on the towing plate of the otter board. It showed 33 to 36 degrees when the point was set at the outmost position, 25 to 31 degrees at the middle position and 19 to 30 degrees at the inmost position, with a decreasing tendency according to the increase of towing speed in each cases. 2. The heel of the otter board always occured inwards, increasing slightly according to the increase of towing speed. 3. The tilt of the otter board always occured outwards, increasing slightly according to the move of the towing point inwards, and decreasing slightly according to the increase of towing speed. 4. The opening between otter boards showed the largest value when the towing point was set at the outmost position.

• Interaction and multiscale mechanics
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• 제5권4호
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• pp.399-405
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• 2012

A dissipative particle dynamics (DPD) simulation was presented to analyze surface wettability and contact angles of a droplet on a solid platform. The many-body DPD, capable of modeling vapor-liquid coexistence, was used to resolve the vapor-liquid interface of a droplet. We found a constant density inside a droplet with a transition along the droplet boundary where the density decreased rapidly. The contact angle of a droplet was extracted from the isosurfaces of the density generated by the marching cube and a spline interpolation of 2D cutting planes of the isosurfaces. A wide range of contact angles from $55^{\circ}$ to $165^{\circ}$ predicted by the normalized parameter ($|A_{SL}|/B_{SL}$) were reported. Droplet with the parameters $|A_{SL}|>5.84B{_{SL}}^{0.297}$ was found to be hydrophilic. If $|A_{SL}|$ was much smaller than $5.84B{_{SL}}^{0.297}$, the droplet was found to be superhydrophobic.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• 제27권1호
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• pp.87-97
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• 1997

The purpose of this study was to evaluate the method for quantitative assessment of bone mass from aluminum-equivalent value of hydroxyapatite by using digital imaging system consisted of Power Macintosh 7200/120, 15-inch color monitor, and GT-9000 scanner with transparency unit. After aluminum-equivalent image made from correlation between aluminum thickness and grey scale, the accuracy of conversion to mass from aluminum-equivalent value was evaluated. Measured bone mass was compared with converted bone mass from aluminum-equivalent value of hydroxyapatite block by correlation formula between aluminum-equivalent value of hydroxyapatite block and hydroxyapatite mass. The results of this study were as follow; 1. Correlation between aluminum thickness and grey level for obtaining aluminum-equivalent image was high positively associated(r²=0.99). Converted masses from aluminum-equivalent value were very similar to measured masses. There was, statistically, no significant difference(P<0.05) between them 2. Correlation between hydroxyapatite aluminum-equivalent and hydroxyapatite mass was shown to linear relation (r²=0.95). 3. Converted masses from aluminum-equivalent value of 3 dry mandible segments were similar to measured masses. The difference between the exposure directions was not significantly different(P<0.05).

• Advances in Traditional Medicine
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• 제5권1호
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• pp.29-36
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• 2005

Polysaccharide hydrogels constitute a structurally diverse class of biological macromolecules with a wide range of physicochemical properties. They also constitute important members of the family of industrial water-soluble polymers. They find application in Pharmacy as binders, disintegrants, suspending, emulsifying and sustaining agents. According to the International Pharmaceutical Excipients Council (IPEC), an excipient must have an established safety profile. Hence, in the present study, in vitro cytotoxicity on Vero and HEp-2 cell lines, and in vivo acute toxicity in rats were carried out to establish the safety of polysaccharide hydrogels from the seeds of Plantago ovata and Ocimum basilicum. The in vitro cytotoxicity was determined by MTT and SRB assays. In the in vivo acute toxicity, the effects of three different doses of hydrogels (100, 200 and 400 mg/kg body weight) on food and water intake, body weight, biochemical and hematological parameters were studied. The results of in vitro did not show any cytotoxicity on both the cell lines used. In the in vivo acute toxicity, the hydrogels did not show any toxic symptoms in all three dose levels. This establishes the safety of the selected hydrogels. Hence, they can be used as excipients in pharmaceutical dosage forms.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• 제25권6_1호
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• pp.485-497
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• 2007

By the rapid development of Information Communication Technology (ICT) and Geo-spatial Technology (GT) and the increased usage of spatial data for planning and infrastructure management, the National Geographic Information System (NGIS) for more efficient and effective utilization of spatial information has been developed by the central government in Korea since 1995. NGIS is the base of Spatial Data Infrastructure (SDI). SDI is developed as one of National Information Infrastructures (NII). Among the hierarchy of SDI, National Spatial Data Infrastructure (NSDI) has very important role in the success of SDI development. Many research articles show that the USA's NSDI initiatives, development strategy have been strongly influenced all over the world. In these viewpoints, to propose the future directions of Korean NGIS, the development of NSDI strategy of USA is reviewed by literature through published book and internet resources. The conclusions of this study are as follow: 1) top-down and bottom-up approach are needed for integrated data sharing and standardization. 2) the creative and evolutionary vision and strategy has to be suggested. 3) the training program and lecture material has to be developed and diffused to the users and providers of spatial data. 4) governance system has to be built for NSDI evaluation. 5) the formation of geo-spatial forum to discuss the spatial-related problems and make research agenda, etc.

• Transactions of the Korean Society of Automotive Engineers
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• 제8권5호
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• pp.36-46
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• 2000

The performance improvement and emission reduction in a turbocharged D.I. diesel engine was studied experimentally in this paper. The system of intake port, fuel injection and turbochager are very important factors which have influence on the engine performance and exhaust emission because the properties in the injected fuel depend on the combustion characteristics. Through these experiments it can be expected to meet performance and emission by optimizing the main parameters; the swirl ratio of intake port, fuel injection system and turbocharger. The swirl ratio of intake port was modified by hand-working and measured by impulse swirl meter. Through this steady flow test, we knew that the increase of swirl ratio is decreasing the mean flow coefficient, whereas the gulf factor is increasing. And the optimum results of engine performance and emission are as follows; the swirl ratio is 2.43, injection timing is BTDC 13。 CA, compression ratio is 16, combustion bowl is re-entrant 5$^{\circ}$, nozzle hole diameter is $\Phi$0.28＊6, turbocharger is GT40 model which are compressor A/R 0.58 AND turbine A/R 1.19.