• Title/Summary/Keyword: $GT_2$

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Effects of Photoperiod Manipulation on Gonadal Activity of the Damselfish, Chromis notata

  • Lee, Chi-Hoon;Park, Young-Ju;Lee, Young-Don
    • Development and Reproduction
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    • v.21 no.2
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    • pp.223-228
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    • 2017
  • This study investigated the effect of different photoperiods (14L: 10D, 12L:12D and 10L:14D) on the gonadal development and GtH mRNA expression in the pituitary of damselfish. The results showed that gonadosomatic index (GSI) was significantly lower in shot photoperiod (10L:14D), in comparison with other photoperiodic group during the spawning season. After 60 days treatment, histological analysis of gonad tissue showed that the gonad of 10L:14D and 12L: 12D treatment groups were resting phase with spermatogonia and perinucleolus stage oocytes but the gonad of 14L:10D treatment group was still ripe phase with spermatozoa and mature stage oocyte. The $FSH{\beta}$ and $LH{\beta}$ mRNA expression in pituitary drastically decreased shot photoperiod treatment from July (spawning period). These results suggest that photoperiod is considered to be the most effective environmental factor in controlling the reproductive cycle of damselfish.

MOLECULAR CLONING OF CHICKEN INTERFERON-GAMMA (닭 인터페론 유전자의 클로닝에 관한 연구)

  • ;Hyun Lillehoj
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 1999.11a
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    • pp.34-50
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    • 1999
  • A cDNA encoding chicken interferon-gamma (chIFN-${\gamma}$) was amplified from P34, a CD4$^{+}$ T-cell hybridoma by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into pUC18. THe sequences of cloned PCR products were determined to confirm the correct cloning. Using this cDNA as probe, chicken genomic library from White Leghorn spleen was screened. Phage clones harboring chicken interferon-gamma (chIFN-${\gamma}$) were isolated and their genomic structure elucidated. The chIFN-${\gamma}$ contains 4 exons and 3 introns spanning over 14 kb, and follows the GT/AG rule for correct splicing at the exon/intron boundaries. The four exons encode 41, 26, 57 and 40 amino acids, respectively, suggesting that the overall structure of IFN-${\gamma}$ is evolutionairly conserved in mammalian and avian species. The 5’-untranslated region and signal sequences are located in exon 1. Several AT-rich sequences located in the fourth exon may indicate a role in mRNA turnover. The 5’-flanking region contains sequences homologous to the potential binding sites for the mammalian transcription factors, activator protein-1(AP-1) activator protein-2(AP-2) cAMP-response element binding protein(CREB), activating transcription factor(ATF), GATA-binding fator(GATA), upstream stimulating factor(USF), This suggests that the mechanisms underlying transcriptional regulation of chicken and mammalian IFN-${\gamma}$ genes may be similar.r.

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Possible Relation between the NOS3 Gene GLU298ASP Polymorphism and Bladder Cancer in Turkey

  • Verim, Levent;Toptas, Bahar;Ozkan, Nazli Ezgi;Cacina, Canan;Turan, Saime;Korkmaz, Gurbet;Yaylim, Ilhan
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.2
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    • pp.665-668
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    • 2013
  • Endothelial nitric oxide synthase (eNOS), encoded by the NOS3 gene, has been suggested to play an important role in uncontrolled cell growth in several cancer types. The objective of this study was to evaluate the role of the NOS3 Glu298Asp polymorphism in bladder cancer susceptibility in a Turkish population. We determined the genotypes of 66 bladder cancer cases and 88 healthy controls. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism analysis. A significant association for NOS3 Glu298Asp heterozygotes genotypes and T allele were found between healthy controls and bladder cancer, respectively (p<0.001: p=0.002). There were no significant associations between any genotypes and the stage, grade, and histological type of bladder cancer. Our study suggested an increased risk role of NOS3 GT genotype in bladder cancer susceptibility in our Turkish population.

A Biomechanical Analysis of 540o Dwihuryeochagi of Taekwondo (태권도 540도 뒤후려차기 동작의 운동역학적 분석)

  • Kang, Dong-Kwon;Kang, Suh-Jung;Yu, Yeon-Joo
    • Korean Journal of Applied Biomechanics
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    • v.23 no.1
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    • pp.19-24
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    • 2013
  • The aim of the study was a quantitative analysis of elite athlete's $540^{\circ}$ Dwihuryeochagi and effects of ground movements to the jumping height and kicking velocity. Eleven elite players(Taekwondo demonstration team) participated in this study. In order to get the kinetic and kinematic variables, ten Vicon cameras and a force plate were used. Foot segment velocity(FSV), vertical ground reaction force(GRF), impulse, ground time(GT) in phase 1, trunk angular velocity(TAV), vertical center of gravity(COG), flight time(FT) in phase 2 and kicking leg segment velocity(KSV) in phase 3 were measured and analyzed. Results indicated that there were similar patterns of variables among phases between subjects. Non-significant correlation(r=.145) between flight time(FT) and impulse was found. Also non-significant correlation(r=.119) between center of gravity(COG) and impulse was found. In conclusions, there were similar strategies in phase 1, phase 2, and phase 3 between subjects.

Performance Predictions of Tilting Pad Journal Bearing with Ball-Socket Pivots and Comparison to Published Test Results (볼 소켓형 피봇을 갖는 틸팅 패드 저널 베어링의 성능 예측 및 기존 결과와의 비교)

  • Kim, Tae Ho;Choi, Tae Gyu
    • The KSFM Journal of Fluid Machinery
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    • v.20 no.2
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    • pp.63-68
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    • 2017
  • This paper predicts the rotordynamic force coefficients of tilting pad journal bearings (TPJBs) with ball-socket pivot and compares the predictions to the published test data obtained under load-between-pad (LBP) configuration. The present TPJB model considers the pivot stiffness calculated based on the Hertzian contact stress theory. Due to the compliance of the pivot, the predicted journal eccentricity agree well with the measured journal center trajectory for increasing static loads, while the early prediction without pivot model consideration underestimates it largely. The predicted pressure profile shows the significant pressure development even on the unloaded pads along the direction opposite to the loading direction. The predicted stiffness coefficients increase as the static load and the rotor speed increase. They agree excellently with test data from open literature. The predicted damping coefficients increase as the static load increases and the rotor speed decreases. The prediction underestimates the test data slightly. In general, the current predictive model including the pivot stiffness improves the accuracy of the rotordynamic performance predictions when compared to the previously published predictions.

A techno-economic analysis of partial repowering of a 210 MW coal fired power plant

  • Samanta, Samiran;Ghosh, Sudip
    • Advances in Energy Research
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    • v.3 no.3
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    • pp.167-179
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    • 2015
  • This paper presents a techno-economic analysis of a partial repowering scheme for an existing 210 MW coal fired power plant by integrating a gas turbine and by employing waste heat recovery. In this repowering scheme, one of the four operating coal mills is taken out and a new natural gas fired gas turbine (GT) block is considered to be integrated, whose exhaust is fed to the furnace of the existing boiler. Feedwater heating is proposed through the utilization of waste heat of the boiler exhaust gas. From the thermodynamic analysis it is seen that the proposed repowering scheme helps to increase the plant capacity by about 28% and the overall efficiency by 27%. It also results in 21% reduction in the plant heat rate and 29% reduction in the specific $CO_2$ emissions. The economic analysis reveals that the partial repowering scheme is cost effective resulting in a reduction of the unit cost of electricity (UCOE) by 8.4%. The economic analysis further shows that the UCOE of the repowered plant is lower than that of a new green-field power plant of similar capacity.

Effect of Luteinising Hormone Releasing Hormone Analogue and Human Chorionic Gonadotropin on Spermiation in the Tongue Sole, Cynoglossus semilaevis (Luteinising Hormone Releasing Hormone Analogue과 Human Chorionic Gonadotropin 처리가 박대(Cynoglossus semilaevis)의 배정에 미치는 영향)

  • LIM, Han-Kyu;PARK, Jung-Yeol;KANG, Hee-Woong
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.2
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    • pp.596-602
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    • 2016
  • Experiments were carried out examine short-term effects of human chorionic gonadotropin (hCG) and long-term effects of luteinising hormone releasing hormone analogue (LHRHa) on milt production of the tongue sole Cynoglossus semilaevis. In the first experiment, each fish was implanted with a blank cholesterol pellet (control), 100 and $200{\mu}g$ LHRHa per kg body weight. In the second experiment, fish were injected with either 100, 200, 400 and 800 IU hCG per kg body weight or same volume of marine fish Ringer's solution. In the first experiment, milt volume was increased in male implanted with $200{\mu}g$ LHRHa pellet compared with other groups at day 10. Injection of 400 and 800 IU hCG resulted in an increase in the milt volume at hour 96 after the treatment. Although statistical difference is unable to confirm because of small milt volume, compared with the control group, hormone pellet-treated groups had a reduction in the mean spermatocrit (Sct) and sperm concentration (Sc). The results suggest that the increase in milt volume is at least partially gonadotropin (GtH)-dependent and increased milt volume has a relationship with milt hydration.

Load Resistance Influence of Magnetoelectric Characteristics on NiZnFe2O4+PZT Composites for Magnetoelectric Sensors

  • Ryu, Ji-Goo;Chung, Su-Tae
    • Journal of Sensor Science and Technology
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    • v.22 no.6
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    • pp.379-386
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    • 2013
  • The influences of the load resistance $R_L$ on the magnetoelectric (ME) characteristics of $NiZnFe_2O_4+PZT$ composite were investigated in the non-resonance frequency range. The ME coefficient peak increases with increasing $R_L$, but the frequency indicating the ME coefficient peak decreases with increasing $R_L$. The maximum output power peak is approximately $9.3{\times}10^{-10}mW/Oe$ near $R_L=3.3M{\Omega}$ at f=280 Hz, and the ME coefficient seems to be saturated at $R_L&gt;20M{\Omega}$. This frequency shift effect of $R_L$ shows that the frequency range for an ME sensor application can be modulated with the appropriate value of $R_L$. The ME output voltage has a good linear response to the ac field Hac and shows fair stability over a range of temperatures. The measured non-linearity of this sample is approximately 0.8%. This sample will allow for a low-strength magnetic ac-field sensor. The result from this sample will serve as basic data for a signal-processing circuit system.

Shaping Ability of Four Rotary Nickel-Titanium Instruments to Prepare Root Canal at Danger Zone (네 가지 전동 Ni-Ti 파일의 danger zone에서의 근관성형력)

  • Choi, Seok-Dong;Jin, Myoung-Uk;Kim, Ki-Ok;Kim, Sung-Kyo
    • Restorative Dentistry and Endodontics
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    • v.29 no.5
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    • pp.446-453
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    • 2004
  • The aim of this study was to evaluate the shaping abilities of four different rotary nickel-titanium instruments with anticurvature motion to prepare root canal at danger zone by measuring the change of dentin thickness in order to have techniques of safe preparation of canals with nickel-titanium files. Mesiobuccal and mesiolingual canals of forty mesial roots of extracted human lower molars were instrumented using the crown-down technique with ProFile, $GT^{TM}$ Rotary file, Quantec file and $ProTaper^{TM}$. In each root, one canal was prepared with a straight up-and-down motion and the other canal was with an anticurvature motion. Canals were instrumented until apical foramens were up to size of 30 by one operator. The muffle system was used to evaluate the root canal preparation. After superimposing the pre- and post-instrumentation canal. change in root dentin thickness was measured at the inner and outer sides of the canal at 1. 3, and 5 mm levels from the furcation. Data were analyzed using two-way ANOVA. Root dentin thickness at danger zone was significantly thinner than that at safe zone at all levels (p < 0.05). There was no significant difference in the change of root dentin thickness between the straight up-and-down and the anticurvature motions at both danger and safe zones in all groups (p > 0.05). ProTaper removed significantly more dentin than other files especially at furcal 3 mm level of danger and safe zones (p < 0.05) Therefore, it was concluded that anticurvature motion with nickel-titanium rotary instruments does not seem to be effective in danger zone of lower molars.

Fishing Experiment for Development of Unused Fishery Resources on the Deep Sea Bed of Korean East Sea (동해구 심해 미이용 자원의 어획 시험 연구)

  • Lee, Byoung-Gee;Lee, Ju-Hee;Shin, Hyeong-Il
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.22 no.4
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    • pp.61-70
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    • 1986
  • In accordance with a rapid growth of demani on aquatic animals, researches of the unused fishery resources On the deep sea b~d in the Korean Waters has been and will be required. The authors carried out a series of fishing experiments to investigate the available resources and to find the effective fishing method on the deep sea bed of the Korean East Sea. In the experiments, 19 kinds of traps which are different from each other in shape, mesh size and entrance diameter were used. The fishing experiments w~r;; carried out in four areas of 20Dm, 600.'11, 800m and 1000.'11 deep respectively, by the Pusan 402 (30:) GT) and the Pusan 403 (279GT), the training ships of National Fisheries University of Pusan, during August, 1986. The catc~ were analyzed with the size, the depth and the construction of traps. The results obtained can be summarized as follows: 1. Main species of the catch w~re pink shrimp, Pandalus bolelis, a kind of welks, Buccimum striatissimum and a kind of larg~ crabs. Chiono8cetes japonicus and the another species were few. 2. The CPUE value (expressed by the number of catch per trap in this paper) of pink shrimp was the highest in the depth of 20J-n around, and the value in the depth of 600.'11 or more decreased gradually with an increase of the depth. But, the value of Buccimum straitissimum was much higher in the depth of 6:J:)!1I or more than that in the depth of 200m around. On the other hand, the value of Chion:Jecetes japonjcus was very low in general. 3. The iniividual body size of the catch differed with the depth. Pink shrimps caught in the depth of 200m around were smaller than those in the depth of 600.'11 or more. In contrast with this, Baccimum striatissim:t.m caught in the depth of 200m around were larger than those in the depth of 600.'11 or more. 4. Depending on the selection curve in Ishida's method for the mesh size of trap webbing, the carapace length of pink shrimp and the shell length of Buccimum striatissimum which are equivalent to 100% relative catching efficiency can be estimated about 3.5cm and 6.5cm or so respectively. 5. The number of catch of pink shrimp and Buccimum striatissimum by the 60.'1'1.'11 entrance diameter of trap were less than that by the 90mm, 120mm and 150mm, even thogh the diffierence am~r, g 9:Jmm, 120.'11.'11 and 150:1'.'11 are not so large.

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