• Journal of Pharmacopuncture
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• v.3 no.2
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• pp.55-77
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• 2000

This is the study of the effects of Aqua-acupuncture with Gardeniae Fructus on thc recovery of rat's liver which was damaged by 0.3ml/ea of $CCI_4$. Rats were divided into 4 groups; Normal-group(None treated group), Control-group(Not treated after $CCI_4$-intoxicated), Exp. I(Treated with Saline Aqua-acupuncture after $CCI_4$-intoxicated) and Exp. ll(Treated with Gardeniae Fructus Aqua-acupuncture after $CCI_4$-intoxicated). Biochemical assays for each serum enzyme activities of AST, ALT, Albumin, LDH, ${\gamma}$-GT, TG and Total cholesterol were performed. The results were summarized as follows: 1. AST activities in serum significantly decreased in the Gardeniae Fructus Aqua-acupuncture treated group after $CCI_4$-intoxicated. In companson with Saline-treated group after $CCI_4$-intoxicated, the Gardeniae Fructus Aqua-acupuncture treated group *The professor of Dept. of Acupuncture & Moxibustion, 2. At T activities in serum significantly decreased in the Gardeniae Fructus Aqua-acupuncture treated group after $CCI_4$-intoxicated. In com pan son with Saline-treated group after $CCI_4$-intoxicated, the Gardeniae Fructus Aqua-acupuncture treated group after $CCI_4$-intoxicated worked effectively to rat's damaged liver. 3. Albumin in serum increased in the Gardeniae Fructus Aqua- acupurkture treated group after $CCI_4$-intoxicated. 4. LDH in serum significantly decreased in the Gardeniae Fructus Aqua-acupuncture treated group after $CCI_4$-intoxicated. In comparison with Saline-treated group after CClcintox icated, the Gardeniae Fructus Aqua acupuncture treated group after $CCI_4$-intoxicated worked highly effectively to rat's damaged liver. 5. ${\gamma}$-GT In serum significantly decreased In the Gardeniae Fructus Aqua-acupuncture trea ted group after $CCI_4$-intoxicated. In compan son with Saline-treated group after $CCI_4$-intoxicated, the Crardeniae Fructus Aqua-acupuncture treated group after $CCI_4$-intoxicated was not recognized significantly. 6. TG in serum significantly decreased in the Gardeniae Fructus Aqua-acupuncture treated group after $CCI_4$-intoxicated. In comparison with Saline-treated group after $CCI_4$-intoxicated, the Gardeniae Fructus Aqua -acupuncture treated group after $CCI_4$-intoxicated worked highly effectively to rat's damaged liver. 7. Total cholesterol in serum decreased in the Gardenias Fructus Aqua-acupuncture treated group after $CCI_4$-intoxicated. In comparison with Saline-treated group after $CCI_4$-intoxicated, the Gardeniae Fructus Aqua-acupuncture treated group after $CCI_4$-intoxicated worked highly effectively to rat's damaged liver. The results from ahove show that the Gardeniae Fructus Aqua- acupuncture has highly effects on the damaged liver caused by $CCI_4$. Therefore it is expected that the Gardeniae Fructus Aqua- acupuncture could be used to cure the damaged liver.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.6
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• pp.3015-3020
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• 2016

Background: Numerous studies have investigated associations of DNA methyltransferase (DNMT) -149 C>T and -579 G>T polymorphisms with gastric cancer (GC) and colorectal cancer (CRC) susceptibility; however, the findings are inconsistent prompting the present meta-analysis. Materials and Methods: Related studies were identified from PubMed, Google scholar, and SID until 10 October 2015. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of the associations. Results: Eleven studies were included based on the search criteria for CRC and GC related to the DNMT3B 149 C>T (3,353 cases and 4,936 controls) and DNMT3B 579 G>T (1,387 cases and 2,064 controls) polymorphisms. There was no significant association overall between DNMT3B -149 and 579 polymorphisms and the risk of cancer. In the stratified analysis by cancer type, DNMT3B 579G>T polymorphism was associated with the risk of CRC and GC. While the DNMT3B -149C/T polymorphism was related with a significantly increased risk of CRC in two tested models, dominant (GG+GT vs. TT: OR 0.51, 95 % CI 0.38-0.69; P = 0.00, Pheterogeneity=0.69, $I^2=0%$) and heterozygote (GT vs. TT: OR 0.50, 95 % CI 0.37-0.69; P=0.00, Pheterogeneity=0.41, $I^2=0%$), no evidence of any association with GC risk was observed as in the pooled analyses. Conclusions: More studies are needed to assess associations of DNMT3B -149C/T and DNMT3B 579G>T polymorphisms with cancer in different ethnicities with large population sizes to generate comprehensive conclusions.

• Experimental and Molecular Medicine
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• v.50 no.12
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• pp.1.1-1.14
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• 2018

DNA methylation is a regulatory mechanism in epigenetics that is frequently altered during human carcinogenesis. To detect critical methylation events associated with gastric cancer (GC), we compared three DNA methylomes from gastric mucosa (GM), intestinal metaplasia (IM), and gastric tumor (GT) cells that were microscopically dissected from an intestinal-type early gastric cancer (EGC) using methylated DNA binding domain sequencing (MBD-seq) and reduced representation bisulfite sequencing (RRBS) analysis. In this study, we focused on differentially methylated promoters (DMPs) that could be directly associated with gene expression. We detected 2,761 and 677 DMPs between the GT and GM by MBD-seq and RRBS, respectively, and for a total of 3,035 DMPs. Then, 514 (17%) of all DMPs were detected in the IM genome, which is a precancer of GC, supporting that some DMPs might represent an early event in gastric carcinogenesis. A pathway analysis of all DMPs demonstrated that 59 G protein-coupled receptor (GPCR) genes linked to the hypermethylated DMPs were significantly enriched in a neuroactive ligand-receptor interaction pathway. Furthermore, among the 59 GPCRs, six GI hormone receptor genes (NPY1R, PPYR1, PTGDR, PTGER2, PTGER3, and SSTR2) that play an inhibitory role in the secretion of gastrin or gastric acid were selected and validated as potential biomarkers for the diagnosis or prognosis of GC patients in two cohorts. These data suggest that the loss of function of gastrointestinal (GI) hormone receptors by promoter methylation may lead to gastric carcinogenesis because gastrin and gastric acid have been known to play a role in cell differentiation and carcinogenesis in the GI tract.

• Journal of Ginseng Research
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• v.43 no.1
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• pp.116-124
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• 2019

Background: Ginsenosides are known as the principal pharmacological active constituents in Panax medicinal plants such as Asian ginseng, American ginseng, and Notoginseng. Some ginsenosides, especially the 20(R) isomers, are found in trace amounts in natural sources and are difficult to chemically synthesize. The present study provides an approach to produce such trace ginsenosides applying biotransformation through Escherichia coli modified with relevant genes. Methods: Seven uridine diphosphate glycosyltransferase (UGT) genes originating from Panax notoginseng, Medicago sativa, and Bacillus subtilis were synthesized or cloned and constructed into pETM6, an ePathBrick vector, which were then introduced into E. coli BL21star (DE3) separately. 20(R)-Protopanaxadiol (PPD), 20(R)-protopanaxatriol (PPT), and 20(R)-type ginsenosides were used as substrates for biotransformation with recombinant E. coli modified with those UGT genes. Results: E. coli engineered with $GT95^{syn}$ selectively transfers a glucose moiety to the C20 hydroxyl of 20(R)-PPD and 20(R)-PPT to produce 20(R)-CK and 20(R)-F1, respectively. GTK1- and GTC1-modified E. coli glycosylated the C3-OH of 20(R)-PPD to form 20(R)-Rh2. Moreover, E. coli containing $p2GT95^{syn}K1$, a recreated two-step glycosylation pathway via the ePathBrich, implemented the successive glycosylation at C20-OH and C3-OH of 20(R)-PPD and yielded 20(R)-F2 in the biotransformation broth. Conclusion: This study demonstrates that rare 20(R)-ginsenosides can be produced through E. coli engineered with UTG genes.

• The Journal of Engineering Geology
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• v.33 no.2
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• pp.307-322
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• 2023

Rock-mass grouting plays a crucial role in the construction of dams and deep caverns, effectively preventing seepage in the foundations, enhancing stability, and mitigating hazards. Most rock grouting is affected by hydrogeological and rock engineering indices such as rock quality designation (RQD), rock mass quality (Q-value), geological strength index (GSI), joint spacing (Js), joint aperture (Ap), lugeon value (Lu), secondary permeability index (SPI), and coefficient of permeability (K). Therefore, accurate geological analysis of basic rock properties and guidelines for grouting construction are essential for ensuring safe and effective grouting design and construction. Such analysis has been applied in dam construction sites, with a particular focus on the geological characteristics of bedrock and the development of prediction methods for grout take. In South Korea, many studies have focused on grout injection materials and construction management techniques. However, there is a notable lack of research on the analysis of hydrogeological and rock engineering information for rock masses, which are essential for the development of appropriate rock grouting plans. This paper reviews the current state of research into the correlation between the grout take with important hydrogeological and rock engineering indices. Based on these findings, future directions for the development of rock grouting research in South Korea are discussed.

• Research in Plant Disease
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• v.14 no.1
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• pp.21-25
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• 2008

This study was conducted to decide disease incidence level of rice leaf blast required for reasonable fungicide application in paddy field. We induced the disease development by inoculating rice blast pathogens on rice seedlings (Jinmibyeo) in the greenhouse and transplanting the infected seedlings in the field two weeks after rice plants were transplanted. We scored the disease incidence, grouped and marked according to degree of percentage of diseased leaf area at maximum stage of disease development. The percent diseased leaf area (PDLA) had negative correlations with panicle number per hill, ripened grain (%), and total yield; their correlation coefficients (r), $-0.97^{**}$, $-1.00^{**}$ and $-0.96^{**}$, respectively. However, it had positive correlations with spikelets per panicle and thousand grain weight; their correlation coefficients (r), $0.98^{**}$ and $0.98^{**}$, respectively. Gain threshold (GT) calculated based on control cost and market price was estimated to be 8.35. Economic injury level (EIL) obtained based on GT and coefficient of damage of regression equation between disease incidence and the different yield components; panicle number per hill, spikelets per panical, ripened grain(%), thousand grain weight and yield were 41.8, 9.7, 19.1, 291.1 and 3.4%, respectively. Economic threshold (ET) for yield was 2.7% ($3.4%(EIL){\times}0.8$) on PDLA. These results suggest that application of fungicide is necessary when two under leaves are almost covered with lesions or contained more than twenty large lesions under leaves at maximum tillering stage.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.22 no.3
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• pp.23-28
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• 1986

This paper describes on the measurement of the deck vibration produced by the main engine vibration of stern trawler MjS SAE-BA-DA (2,275GT, 3,600PS) while the ship is cruising and drifting. The obtained results are as follows; 1. The deck vibration level was the highest point at vertical line which pass main engine and the lowest point at vertical line which pass top bridge while the crusing. 2. The vibration source level of the main engine, screw shaft and screw propeller were respectively 110, 90 and 80% while the crusing. 3. The main deck vibration pressure level at the check points 2, 20, 30, 40, 60, 70, 80, 86m from the bow to stern was respectively 9, 8, 7, 10, 22, 45, 18, 23%. 4. The frequency distributions of the rr.ain engine, screw shaft, screw propeller vibration were from 3 Hz to 10 KHz, predominant frequency was 1 KHz, each vibration accelration the highest level were respectively 1. 3, 0.8, 0.5 $mm/s^2.$ 5. The predominant frequency distributions of the main deck, second deck, bridge deck and top bridge deck-s vibration were from 10 to 30 Hz, and each vibration accelration level were respectively 0.7, 0.05, 0.07, 0.04 $mm/s^2.$

• Journal of Korea Fishing Vessel Association
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• v.29
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• pp.15-20
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• 1986

This paper describes on the measurement of the deck vibration produced by the main engine vibration of stern trawler MIS SAE-BA-DA (2,275GT, 3,600PS) while the ship is cruising and drifting. The obtained results are as follows; 1. The deck vibration level was the highest point at vertical line which pass main engine and the lowest point at vertical line which pass top bridge while the crusing. 2. The vibration source level of the main engine, screw shaft and screw propeller were respectively 110, 90 and 80% while the crusing. 3. The main deck vibration pressure level at the check points 2, 20, 30, 40, 60, 70, 80, 86m from the bow to stern was respectively 9, 8, 7, 10, 22, 45, 18, 23%. 4. The frequency distributions of the main engine, screw shaft, screw propeller vibration were from 3Hz to 10KHz, predominant frequency was 1KHz, each vibration accelration the highest level were respectively 1.3, 0.8, 0.5mm/$S^2$. 5. The predominant frequency distributions of the main deck, second deck, bridge deck and top bridge deck's vibration were from 10 to 30Hz, and each vibration accelration level were respe¬ctively 0.7, 0.05, 0.07, 0.04mm/$S^2$.

• Journal of the FoodService Safety
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• v.2 no.2
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• pp.91-96
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• 2021

This study was performed to provide fundamental data on hygiene and quality control of ready-to-eat sandwiches. Predictive models were developed to the kinetics of Staphylococcus aureus growth in these sandwiches as a function of temperature (10, 15, 25, and 35℃). The result of the primary model that used the Gompertz equation showed that the lag phase duration (LPD) and generation time (GT) decreased and the exponential growth rate (EGR) increased with increasing storage temperature. The secondary model showed an R² for M and B of 0.9967 and 09916, respectively. A predictive growth model of the growth degree as a function of temperature was developed. L(t)=A+Cexp(-exp(-B(t-M))) (A=Initial contamination level, C=MPD-A, B=0.473166-0.045040*Temp-0.001718*Temp*Temp, M=19.924824-0.627442*Temp-0.004493*Temp*Temp, t=time, Temp=temperature). This model showed an R² value of 0.9288. All the models developed in this study showed a good fit.

• Journal of applied mathematics & informatics
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• v.31 no.5_6
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• pp.669-676
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• 2013

Let $a$ and $b$ be two even integers with $2{\leq}a<b$, and let k be a nonnegative integer. Let G be a graph of order $n$ with $n{\geq}\frac{(a+b-1)(a+b-2)+bk-2}{b}$. A graph G is called an ($a,b,k$)-critical graph if after deleting any $k$ vertices of G the remaining graph of G has an [$a,b$]-factor. In this paper, it is proved that G is an ($a,b,k$)-critical graph if $${\mid}N_G(X){\mid}&gt;\frac{(a-1)n+{\mid}X{\mid}+bk-2}{a+b-1}$$ for every non-empty independent subset X of V (G), and $${\delta}(G)>\frac{(a-1)n+a+b+bk-3}{a+b-1}$$. Furthermore, it is shown that the result in this paper is best possible in some sense.