• Title/Summary/Keyword: $GT_1$

Search Result 522, Processing Time 0.026 seconds

Characterization and refinement of enzyme of the gene encoding catechol 1,2-dioxygenase from Phenol-degrading, Rhodococcus sp.

  • 이희정;박근태;박재림;이상준
    • Proceedings of the Korean Environmental Sciences Society Conference
    • /
    • 2002.05b
    • /
    • pp.209-212
    • /
    • 2002
  • The heavy use of petroleum products in modern livings has brought ubiquitous environmental contaminants of aromatic compounds, which persist in aquatic and geo-environment without the substantial degradation. The persistence and accumulation of the aromatic compounds, which include xylene, phenol, toluene, phthalate, and so on are known to cause serious problems in our environments. Some of soil and aquatic microorganisms facilitate their growth by degrading aromatic compounds and utilizing degrading products as growth substrates, the biodegradation helps the reentry of carbons of aromatic compounds, preventing their accumulation in our environments. The metabolic studies on the degradation of aromatic compounds by microoganlsms were extensively carried out along with their genetic studies. A Rhodococcus sp. isolated in activated sludges has shown the excellent ability to grow on phenol as a sole carbon source. In the present study investigated a gene encoding phenol-degrading enzymes from a Rhodococcus sp.

  • PDF

On the Hull Vibration of the Training Ship Sae-Ba-Da (실습선 새바다호의 선체진동에 관하여)

  • 박중희
    • Journal of the Korean Society of Fisheries and Ocean Technology
    • /
    • v.22 no.3
    • /
    • pp.23-28
    • /
    • 1986
  • This paper describes on the measurement of the deck vibration produced by the main engine vibration of stern trawler MjS SAE-BA-DA (2,275GT, 3,600PS) while the ship is cruising and drifting. The obtained results are as follows; 1. The deck vibration level was the highest point at vertical line which pass main engine and the lowest point at vertical line which pass top bridge while the crusing. 2. The vibration source level of the main engine, screw shaft and screw propeller were respectively 110, 90 and 80% while the crusing. 3. The main deck vibration pressure level at the check points 2, 20, 30, 40, 60, 70, 80, 86m from the bow to stern was respectively 9, 8, 7, 10, 22, 45, 18, 23%. 4. The frequency distributions of the rr.ain engine, screw shaft, screw propeller vibration were from 3 Hz to 10 KHz, predominant frequency was 1 KHz, each vibration accelration the highest level were respectively 1. 3, 0.8, 0.5 $mm/s^2.$ 5. The predominant frequency distributions of the main deck, second deck, bridge deck and top bridge deck-s vibration were from 10 to 30 Hz, and each vibration accelration level were respectively 0.7, 0.05, 0.07, 0.04 $mm/s^2.$

  • PDF

Study On The Midwater Trawl Available in the Korean Waters - 1 . Attitude and Opening Opening Efficiency of Otter Board - (한국 근해에 있어서의 중층 트로올의 연구 - 1 . 전개판의 동작상태의 전개성능 -)

  • 이병기
    • Journal of the Korean Society of Fisheries and Ocean Technology
    • /
    • v.22 no.4
    • /
    • pp.41-48
    • /
    • 1986
  • To investigate the midwater trawl gear available for the Korean near sea trawlers, the authors carried out a field experiment on the attitude and the opening efficiency of the otter board with the Pusan 404 (160GT, 750ps), a training ship of National Fisheries University of Pusan. The experimental trawl gear was designed to be operable by the ship and the otter board was made of single iron plate with 12% camber ratio. The special-prepared potentiometric angle detector was used for determining the attitude, and the 50 KHz fish finder for the opening between the otter boards. The results obtained can be summarized as follows: 1. The angle of attack varied by moving the towing point on the towing plate of the otter board. It showed 33 to 36 degrees when the point was set at the outmost position, 25 to 31 degrees at the middle position and 19 to 30 degrees at the inmost position, with a decreasing tendency according to the increase of towing speed in each cases. 2. The heel of the otter board always occured inwards, increasing slightly according to the increase of towing speed. 3. The tilt of the otter board always occured outwards, increasing slightly according to the move of the towing point inwards, and decreasing slightly according to the increase of towing speed. 4. The opening between otter boards showed the largest value when the towing point was set at the outmost position.

  • PDF

On the Hull Vibration of the Training Ship Sae-Ba-Da (실습선 새바다호의 선체진동에 관하여)

  • Park, Jung-Hui
    • Journal of Korea Fishing Vessel Association
    • /
    • v.29
    • /
    • pp.15-20
    • /
    • 1986
  • This paper describes on the measurement of the deck vibration produced by the main engine vibration of stern trawler MIS SAE-BA-DA (2,275GT, 3,600PS) while the ship is cruising and drifting. The obtained results are as follows; 1. The deck vibration level was the highest point at vertical line which pass main engine and the lowest point at vertical line which pass top bridge while the crusing. 2. The vibration source level of the main engine, screw shaft and screw propeller were respectively 110, 90 and 80% while the crusing. 3. The main deck vibration pressure level at the check points 2, 20, 30, 40, 60, 70, 80, 86m from the bow to stern was respectively 9, 8, 7, 10, 22, 45, 18, 23%. 4. The frequency distributions of the main engine, screw shaft, screw propeller vibration were from 3Hz to 10KHz, predominant frequency was 1KHz, each vibration accelration the highest level were respectively 1.3, 0.8, 0.5mm/$S^2$. 5. The predominant frequency distributions of the main deck, second deck, bridge deck and top bridge deck's vibration were from 10 to 30Hz, and each vibration accelration level were respe¬ctively 0.7, 0.05, 0.07, 0.04mm/$S^2$.

  • PDF

Comparison of Horizontal and Vertical Noise Power Spectrum in Measurements by Using Various Electronic Portal Imaging Devices in Radiation Therapy (방사선치료 시 전자포털 영상장치를 이용한 잡음전력스펙트럼 수평 및 수직 측정비교)

  • Kim, Ki-Won;Choi, Kwan-Woo;Jeong, Hoi-Woun;Jang, Seo-Goo;Kwon, Kyung-Tae;Son, Soon-Yong;Son, Jin-Hyun;Min, Jung-Whan
    • Journal of radiological science and technology
    • /
    • v.39 no.2
    • /
    • pp.171-176
    • /
    • 2016
  • The quality assurance (QA) is very important for diagnostic field and radiation therapy field to evaluate the characteristic of devices. The purpose of this study was to compare different NPS methodologies results which are measuring NPS with regard to horizontal and vertical directions by using megavoltage X-ray energies. The NPS evaluation methods were applied to the International Electro-technical Commission standard (IEC 62220-1). The electronic portal imaging devices (EPID) devices such as Siemens BEAMVIEW$^{PLUS}$, Elekta iViewGT and Varian Clinac$^R$ iX aS1000 were used. NPS data were expressed by corresponding each frequency about average of noise value corresponding the each frequency, and NPS were evaluated quantitatively by totaling up the noise values of average frequency which are on horizontal and vertical directions. In NPS results for Elekta iViewGT, NPS of horizontal and vertical by using 4 methods were indicated the difference of 3~5% between horizontal and vertical direction. In the results of Siemens BEAMVIEW$^{PLUS}$ and Varian Clinac$^R$ iX aS1000, the NPS of horizontal and vertical direction were indicated the difference of 15% when averaging the whole values. This study were evaluated the NPS of each devices by totaling up the noise values of average frequency which are on horizontal and vertical directions suggesting the quantitative evaluation method using the data.

DNA-functionalized single-walled carbon nanotube-based sensor array for gas monitoring

  • Zhang, Wenjun;Liu, Yu;Wang, Ming. L
    • Smart Structures and Systems
    • /
    • v.12 no.1
    • /
    • pp.73-95
    • /
    • 2013
  • Nine deoxyribonucleic acid (DNA) sequences were used to functionalize single-walled carbon nanotube (SWNT) sensors to detect the trace amount of methanol, acetone, and HCl in vapor. DNA 24 Ma (24 randomly arranged nitrogenous bases with one amine at each end of it) decorated SWNT sensor and DNA 24 A (only adenine (A) base with a length of 24) decorated SWNT sensor have demonstrated the largest sensing responses towards acetone and HCl, respectively. On the other hand, for the DNA GT decorated SWNT sensors with different sequence lengths, the optimum DNA sequence length for acetone and HCl sensing is 32 and 8, separately. The detection of methanol, acetone, and HCl have identified that DNA functionalized SWNT sensors exhibit great selectivity, sensitivity, and repeatability with an accuracy of more than 90%. Further, a sensor array composed of SWNT functionalized with various DNA sequences was utilized to identify acetone and HCl through pattern recognition. The sensor array is a combination of four different DNA functionalized SWNT sensors and two bare SWNT sensors (work as reference). This wireless sensing system has enabled real-time gas monitoring and air quality assurance for safety and security.

Nucleotide Sequence of Rat Transketolase and Liver-Specific Pretranslational Activation During Postnatal Development

  • Kim, Sung-Min F.;Kim, Byung-Moon;Jeng, Jingjau;Soh, Yun-Jo;Bak, Choong-Il;Huh, Jae-Wook;Song, Byoung-J.
    • BMB Reports
    • /
    • v.29 no.2
    • /
    • pp.146-150
    • /
    • 1996
  • A 2.1 kb cDNA clone for rat transketolase was isolated from rat liver ${\lambda}gt11$ cDNA library and its sequence was determined. The predicted rat transketolase (655 amino acids with $M_r$ 71,186) is highly similar (92%) to that of the human enzyme except that it contains an extra 32 amino acids at its N-terminus. Although it is less similar (<27%) to transketolases from non-mammalian species, the functional motifs such as the catalytic sites and thiamine binding domain are well conserved in the rat enzyme. Southern blot analysis of genomic DNA verified that transketolase appears to be derived from a single gene. Immunoblot and Northern blot analyses suggested that hepatic transketolase was activated pretranslationally by a 2.1-fold while little change was observed in brain enzyme, indicating a tissue-specific pretranslational activation during postnatal development.

  • PDF

Molecular Characterization of Rockbream (Oplegnathus fasciatus) Cytoskeletal β-actin Gene and Its 5'-Upstream Regulatory Region

  • Lee, Sang-Yoon;Kim, Ki-Hong;Nam, Yoon-Kwon
    • Fisheries and Aquatic Sciences
    • /
    • v.12 no.2
    • /
    • pp.90-97
    • /
    • 2009
  • The cytoskeletal $\beta$-actin gene and its 5'-upstream region were isolated and characterized in the rockbream (Oplegnathus fasciatus). Complementary DNA of the rockbream $\beta$-actin represented a 1,125 bp of an open reading frame encoding 375 amino acids, and the rockbream $\beta$-actin cDNA and deduced amino acid sequences were highly homologous to those of other vertebrate orthologs. At the genomic level, the $\beta$-actin gene also exhibited an organization typical of vertebrate cytoskeletal actin genes (2,159 bp composed of five translated exons interrupted by four introns) with a conserved GT/AG exon-intron splicing rule. The putative non-translated exon predicted in the rockbream $\beta$-actin gene was much more homologous with those of teleostean $\beta$-actin genes than those of mammals. The 5'-upstream regulatory region isolated by genome walking displayed conserved and essential elements such as TATA, CArG and CAAT boxes in its proximal part, while several other immune- or stress-related motifs such as those for NF-kappa B, USF, HNF, AP-1 and C/EBP were in the distal part. Semi-quantitative RT-PCR assay results demonstrated that the rockbream $\beta$-actin transcripts were ubiquitously but different-tially expressed across the tissues of juveniles.

T-NEIGHBORHOODS IN VARIOUS CLASSES OF ANALYTIC FUNCTIONS

  • Shams, Saeid;Ebadian, Ali;Sayadiazar, Mahta;Sokol, Janusz
    • Bulletin of the Korean Mathematical Society
    • /
    • v.51 no.3
    • /
    • pp.659-666
    • /
    • 2014
  • Let $\mathcal{A}$ be the class of analytic functions f in the open unit disk $\mathbb{U}$={z : ${\mid}z{\mid}$ < 1} with the normalization conditions $f(0)=f^{\prime}(0)-1=0$. If $f(z)=z+\sum_{n=2}^{\infty}a_nz^n$ and ${\delta}$ > 0 are given, then the $T_{\delta}$-neighborhood of the function f is defined as $$TN_{\delta}(f)\{g(z)=z+\sum_{n=2}^{\infty}b_nz^n{\in}\mathcal{A}:\sum_{n=2}^{\infty}T_n{\mid}a_n-b_n{\mid}{\leq}{\delta}\}$$, where $T=\{T_n\}_{n=2}^{\infty}$ is a sequence of positive numbers. In the present paper we investigate some problems concerning $T_{\delta}$-neighborhoods of function in various classes of analytic functions with $T=\{2^{-n}/n^2\}_{n=2}^{\infty}$. We also find bounds for $^{\delta}^*_T(A,B)$ defined by $$^{\delta}^*_T(A,B)=jnf\{{\delta}&gt;0:B{\subset}TN_{\delta}(f)\;for\;all\;f{\in}A\}$$ where A, B are given subsets of $\mathcal{A}$.

A study on assessment of bone mass from aluminum-equivalent image by digital imaging system (디지털 영상 시스템을 이용한 알루미늄 당량화상에 의한 골량 측정에 관한 연구)

  • Kim Jin-Soo;Choi Eui-Hwan;Kim Jae-Duk
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
    • /
    • v.27 no.1
    • /
    • pp.87-97
    • /
    • 1997
  • The purpose of this study was to evaluate the method for quantitative assessment of bone mass from aluminum-equivalent value of hydroxyapatite by using digital imaging system consisted of Power Macintosh 7200/120, 15-inch color monitor, and GT-9000 scanner with transparency unit. After aluminum-equivalent image made from correlation between aluminum thickness and grey scale, the accuracy of conversion to mass from aluminum-equivalent value was evaluated. Measured bone mass was compared with converted bone mass from aluminum-equivalent value of hydroxyapatite block by correlation formula between aluminum-equivalent value of hydroxyapatite block and hydroxyapatite mass. The results of this study were as follow; 1. Correlation between aluminum thickness and grey level for obtaining aluminum-equivalent image was high positively associated(r²=0.99). Converted masses from aluminum-equivalent value were very similar to measured masses. There was, statistically, no significant difference(P<0.05) between them 2. Correlation between hydroxyapatite aluminum-equivalent and hydroxyapatite mass was shown to linear relation (r²=0.95). 3. Converted masses from aluminum-equivalent value of 3 dry mandible segments were similar to measured masses. The difference between the exposure directions was not significantly different(P<0.05).

  • PDF