• Journal of Microbiology and Biotechnology
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• 제6권2호
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• pp.142-144
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• 1996

To clone the gene coding for steroid $\Delta^1$-dehydrogenase of Arthrobacter simplex, its genomic library was constructed with a , $\lambda$gt11 expression vector and immunoscreened with antiserum against the enzyme. One positive clone was found to carry a 1.6-kb EcoR I restriction endonuclease fragment of A. simplex DNA. The restriction map of the 1.6-kb EcoR I fragment was determined after cloning of the DNA into pBS vector.

• Journal of Genetic Medicine
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• 제2권1호
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• pp.17-22
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• 1998

The Lesch-Nyhan syndrome which is caused by the deficiency of hypoxanthine guanine phosphoribosyltransferase is an X-linked recessive disorder characterized by hyperuricemia, choreoathetosis, mental retardation and compulsive self-injurious behavior. Clinical management of the patients with the Lesch-Nyhan syndrome is frustrating and requires burdensome medical treatment since it cripples the patient and shortens the life span by progression of neurological symptoms, but there are no cures or measures for relieving relentless natural course of the disease yet. Therefore, prenatal diagnosis of the affected fetus is important in genetic counselling for the family at high risk. In this study, four different mutations in the HPRT gene of four probands have been identified in four unrelated families; K215X, Q109X, nt.631 ${\Delta}A$, and nt.289 ${\Delta}GT$. Two mutations among them altered restriction enzyme sites; SpeI for Q109X and MaeI for nt.289 ${\Delta}GT$. Based on their molecular defects, prenatal diagnoses of 3 the fetuses were successfully made between ninth and eleventh week of gestation by polymerase chain reaction (PCR), restriction digestion and DNA sequencing using cDNA obtained from chorionic villus samples (CVS). We predicted the outcome of all fetuses prenatally. Among the three fetuses two were male and one was female according to the identification made by PCR amplification of the sex determining region of the Y chromosome(SRY) gene. Each carried a wild type allele for the corresponding mutant allele. They were also tested postnatally for the mutations to be unaffected.

• 대한물리의학회지
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• 제9권4호
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• pp.455-463
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• 2014

PURPOSE: The purpose of this study was to compare the effects of Graston and self-myofascial release (SMR) techniques on knee joint flexibility, hamstring, and quadriceps strength. METHODS: Twenty subjects with hamstring shortness participated in this study. The subjects were assigned randomly to one of two groups: The Graston technique (GT) group received intervention using a Graston instrument for one minute, and the SMR group performed self-exercises using a foam roll for one minute. The range of motion (ROM) of the knee joint was measured by active knee extension test, and a handheld dynamometer was utilized to collect the hamstring and quadriceps muscle strength. This experiment was performed by two physical therapists. The significant level was set at ${\alpha}$=0.05. RESULTS: The results were as follows: 1) The ROM of the knee joint and quadriceps muscle strength were significantly increased in both groups. 2) Hamstring muscle strength was significantly reduced in both groups. 3) There were no significant differences between the GT group and SMR group for any variable. CONCLUSION: The results of this study suggest that SMR is an effective and easy technique for restoring proper muscle length and strength in subjects with hamstring shortness. We recommend that SMR technique be used for treat hamstring shortness in clinical setting and home-program.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 총회 및 춘계학술발표회
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• pp.77-82
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• 2004

Quantifying rates of microbial processes under subsurface conditions is difficult, and is most commonly approximated by laboratory studies using aquifer materials. In this study a single-well, 'push-pull' test method is adapted for the in situ determination of denitrification rates in groundwater aquifers. The rates of stepwise reduction of nitrate to nitrite, nitrous oxide, and molecular nitrogen were determined by performing a series of push-pull tests at an experimental well field of Korea University. A single Transport Test, one Biostimulation Test, and four Activity Tests were conducted for this study. Transport tests are conducted to evaluate the mobility of solutes used in subsequent tests. These included bromide (a conservative tracer), fumarate (a carbon and/or source), and nitrate (an electron acceptor). At this site, extraction phase breakthrough curves for all solutes were similar, indicating apparent conservative transport of the solutes prior to biostimulation. Biostimulation tests were conducted to stimulate the activity of indigenous heterotrophic denitrifyinc microorganisms. Biostimulation was detected by the simultaneous production of carbon dioxide and nitrite after each injection. Activity tests were conducted to quantify rates of nitrate, nitrite, and nitrous oxide reduction. Estimated zero-order degradation rates decreased in the order nitrate '||'&'||'gt; nitrite '||'&'||'gt; nitrous oxide. The series of push-pull tests developed and field tested in this study should prove useful for conducting rapid, low-cost feasibi1ity assessments for in situ denitrification in nitrate-contaminated aquifers.

• 신재생에너지
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• 제7권2호
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• pp.10-17
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• 2011

Korea is the 11th largest energy consumption country and 96% of its total energy consumption depends on imports from overseas. Therefore it is a very important task to secure renewable energy sources which can reduce both the carbon-dioxide emission and dependency on overseas energy imports. Among the various renewable energy sources, organic wastes are important sources. In Korea, 113 million toe of methane is generated from organic wastes annually, but only 3.7% is effectively used for energy conversion. Thus, it is very important to make better use of organic wastes, especially for power generation. The goals of this project are to develope the fuel supplying system of Bio Gasturbine (GT) for 5MW-class co-generation system. The fuel supplying system mainly consists of $H_2S$ removal system, Bio Gas compression system, Siloxane removal system and moisture separating systems. The fuel requirement of 5MW-class GT is at around 60% of $CH_4$, $H_2S$ (<30 ppm), Siloxane(<10 mg/$nm^3$) and supply pressure (> 25 bar) from biogas compressor. Main mechnical charateristics of Bio Gasturbine system have the specific performance; 1) high speed turbine speed (12,840 rpm) 2) very clean emmission NOx (<50 ppm) 3) high efficiency of energy conversion rate. This paper focuses on the development of design technology for food waste biogas pretreatment system for 5MW-class biogas turbine. The study also has the plan to replace the fuel of gas turbine and other distributed power systems. As the increase of bioenergy, this system help to contribute to spread more New & Renewable Energy and the establishment of Renewable Portfolio Standards (RPS) for Korea.

• Bulletin of the Korean Chemical Society
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• 제26권4호
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• pp.641-644
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• 2005

Nucleophilic addition reactions of benzylamines $(XC_6H_4CH_2NH_2)\;to\;{\beta}$-cyanostilbenes ($YC_6H_4CH=C(CN)C_6H_4$Y’) have been studied in acetonitrile at 30.0 oC. A greater degree of N-$C_{\alpha}$ bond formation (larger ${\beta}_X$) is obtained with a stronger electron-withdrawing substituent in either ${\alpha}-\;(\delta\sigma_Y\;{\gt}\;0)\;or\;{\beta}-ring\;(\delta\sigma_{Y'}\;{\gt}$ 0). A stronger charge development is observed in the TS on $C_{\beta}\;(\rho_{Y'}$= 1.06 for X=Y=H) rather than on $C_{\alpha}\;(\rho_{Y}$ = 0.62 for X=Y’H) indicating the lag in the resonance development into the activating group (CN) on $C_{\beta}$ in the transition state. Similarly, the magnitude of $\rho$$_{XY'}$(−0.72) is greater than $\rho_{XY}$ (−0.66) due to a stronger interaction of the nucleophile with $\beta$-ring than $\alpha$-ring. The positive sign of $\rho_{YY'}$correctly reflects $\pi$ bond cleavage between the two rings in the TS. Relatively large kinetic isotope effects ($k_H/k_D\;{\geq}$ 2.0) involving deuterated nucleophiles ($XC_6H_4CH_2ND_2$) suggest a four-membered cyclic TS in which concurrent N-C$_{\alpha}$ and H(D)-C$_{\beta}$ bond formation occurs.

• 한국균학회지
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• 제28권1호
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• pp.26-31
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• 2000

팽이버섯의 자실체형성 과정에 특이적으로 발현하는 FVFD16과 FVFD30 유전자의 genomic 클론을 단리하여 염기서열을 분석하였다. FVFD16은 Open Reading Frame(ORF)내에 2개의 intron이 관찰되었고, FVFD30 유전자에서는 4개의 intron이 관찰되었다. 또한 intron의 특징적인 염기서열인 GT/AG의 rule과도 일치하고 있음이 밝혀졌다. FVFD16과 FVFD30의 두 유전자 모두에서 진핵생물의 promoter영역에서 자주 관찰되는 CAAT box와 유사한 배열과 TATA box가 존재했다. 또한, 전사개시점의 바로 앞에서 관찰되어지는 CT-rich의 영역이 존재하고 있었으며, 특히 FVFD30에서는 전사개시 시에 중요한 역할을 할 것으로 예상되는 CCACC의 서열이 관찰되었다. 한편 FVFD16 genomic클론의 염기서열 분석결과 cDNA클론과 80%의 상동성을 나타내는 gene family임이 밝혀졌다. 여러 가지 제한효소에 의한 genomic southern blot 분석결과 FVFD16과 FVFD30은 2번 이상의 반복배열 또는 gene family의 존재가 확인되었다.

• 공학교육연구
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• 제17권4호
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• pp.43-47
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• 2014

The rapid development of science & technology and the globalization of society have accelerated the fractionation and specialization of academic disciplines. Accordingly, Korean colleges and universities are continually dropping antiquated courses to make room for new courses that better meet societal demands. With emphasis placed on providing students with a broader range of choices in terms of course selection, compulsory courses have given way to elective courses. On average, 4 year institutions of higher learning in Korea currently offer somewhere in the neighborhood of 1,000 different courses yearly. The classification of an ever growing list of courses offered and the practical use of such data would not be possible without the aid of computers. For example, if we were able to show the pre/post requisite relationship among various courses as well as the commonalities in substance among courses, such data generated regarding the interrelationship of different courses would undoubtedly greatly benefit the students, as well as the professors, during course registration. Furthermore, the GT system's relatively simple approach to course classification and coding will obviate the need for the development of a more complicated keyword based search engine, and hopefully contribute to the standardization of the course coding scheme in the future..Therefore, as a sample case project, this study will use GT to classify and code all courses offered at the College of Engineering of K University, thereby developing a system that will facilitate the scanning of relevant courses.

• 인터넷정보학회논문지
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• 제7권1호
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• pp.17-30
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• 2006

인터넷과 웹이 활성화됨으로써 사용자는 문서, 디렉토리, 데이터베이스, 웹 페이지 등과 같은 자원들을 액세스하는 것이 훨씬 더 쉬워졌다. 그러나 이로 인하여 네트워크의 인증, 자원들을 액세스하기 위한 권한의 허가, 데이터의 정책과 보안 그리고 보안 시스템의 무결성과 같은 중대한 보안 문제들이 생기게 되었다. 본 논문에서는 기업 환경의 접근제어를 위하여 시간(기간과 주기)에 따른 제약으로 자원의 사용을 제한할 수 있는 GTRBAC(Generalized Temporal Role Based Access Control) 모델에 부역할(sub-role) 개념과 PBDM(Permission Based Delegation Model) 개념을 적용한 확장된 GTRBAC 위임(Ex-GTRBAC Delegation)모델을 제안한다. 제안 모델은 부역할을 사용하여 하위 역할에 할당된 권한을 상위 역할에 할당된 사용자가 모두 상속하여 실행할 수 없도록 하여 권한의 남용을 방지하여 최소권한의 원칙을 지킬 수 있도록 하고, 기업 환경에서 빈번히 발생하는 권한의 위임에 대해서 사용자 대 사용자 위임, 역할 대 역할 위임, 다단계 위임, 다중 위임과 같은 기능을 제공하여 기업 환경의 특성에 따라 다양하고 정교한 접근제어 정책을 적용할 수 있도록 한다.

• Food Science and Biotechnology
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• 제14권1호
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• pp.42-45
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• 2005

The present study was carried out to envisage the aerobic growth of Listeria monocytogenes and Yersinia enterocolitica on pork, which is one of the major meat sources in Korea. The results were compared with the previously developed predictive model systems for the verification of microbial growth in a real situation during pork processing. Pork loin samples (8.0 g, 5 mm thick) were aseptically prepared and inoculated with each pathogen by immersing into the respective inoculums for one min. Each of the samples were then wrapped with PE film and stored at 5, 10, and $15^{\circ}C$ up to 36 days to measure the growth profile of the respective pathogens. The growth parameters were calculated by using Gompertz equation and were compared with the previously reported data. The predicted generation time (GT) of L. monocytogenes at 5, 10 and $15^{\circ}C$ was 28.74, 7.85 and 4.02 hr, respectively, and for Y. enterocolitica was 10.29, 4.74 and 2.50 hr, at the same temperatures respectively. In this study, the GT values predicted on pork were slightly higher than the values predicted in other studies using liquid model systems. Unlike previous reports, both the pathogens were found to grow at $5^{\circ}C$ on pork. This finding recommends the necessity of controlling the growth of both the pathogens during the slaughtering process and distribution.