• Title/Summary/Keyword: $GT_1$

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Protective Effect of Ethanol Extract of Artemisiae vulgaris L. on hepatic injury Induced by Carbon tetrachloride In Rat. (애엽 에탄올 추출물이 사염화탄소로 유발된 흰쥐의 간 손상 보호효과)

  • Kim, Ok-Kyung
    • Journal of the Korean Applied Science and Technology
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    • v.36 no.4
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    • pp.1420-1426
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    • 2019
  • This study was done to investigate the protective effects of ethanol extract Artemisiae vulgaris L(Av) on carbon tetrachloride(CCl4)intoxicated rats. Male sprague Dawley rats(200~210g)was used. experimental groups were divided into normal group, CCl4-control group, and ethanol extract CCl4-treated group. CCl4-treated groups were injected with CCl4 0.6mg/kg.b.w(i.p). The activities of Alanine aminotransferase(ALT), Aspartate aminotransferase(AST), Alkaline phosphatase(ALP), Glutamyl transpeptidase(γ-GT), Lactate dehydrogenase(LDH) in extract pretrated group was significantly decreased(p<0.05) compared to the CCl4-control group. The contents of triglyceride, cholesterol and lipid peroxide were significantly decreased(p<0.05). whereas the contents of HDL-cholesterol and glutathione(GSH) were significantly increased(p<0.05). These results suggest that extract of Artemisiae vulgaris L(Av) has hepatoprotective effect in the CCl4-intoxicated rats.

PREDICTION OF DAILY MAXIMUM X-RAY FLUX USING MULTILINEAR REGRESSION AND AUTOREGRESSIVE TIME-SERIES METHODS

  • Lee, J.Y.;Moon, Y.J.;Kim, K.S.;Park, Y.D.;Fletcher, A.B.
    • Journal of The Korean Astronomical Society
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    • v.40 no.4
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    • pp.99-106
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    • 2007
  • Statistical analyses were performed to investigate the relative success and accuracy of daily maximum X-ray flux (MXF) predictions, using both multilinear regression and autoregressive time-series prediction methods. As input data for this work, we used 14 solar activity parameters recorded over the prior 2 year period (1989-1990) during the solar maximum of cycle 22. We applied the multilinear regression method to the following three groups: all 14 variables (G1), the 2 so-called 'cause' variables (sunspot complexity and sunspot group area) showing the highest correlations with MXF (G2), and the 2 'effect' variables (previous day MXF and the number of flares stronger than C4 class) showing the highest correlations with MXF (G3). For the advanced three days forecast, we applied the autoregressive timeseries method to the MXF data (GT). We compared the statistical results of these groups for 1991 data, using several statistical measures obtained from a $2{\times}2$ contingency table for forecasted versus observed events. As a result, we found that the statistical results of G1 and G3 are nearly the same each other and the 'effect' variables (G3) are more reliable predictors than the 'cause' variables. It is also found that while the statistical results of GT are a little worse than those of G1 for relatively weak flares, they are comparable to each other for strong flares. In general, all statistical measures show good predictions from all groups, provided that the flares are weaker than about M5 class; stronger flares rapidly become difficult to predict well, which is probably due to statistical inaccuracies arising from their rarity. Our statistical results of all flares except for the X-class flares were confirmed by Yates' $X^2$ statistical significance tests, at the 99% confidence level. Based on our model testing, we recommend a practical strategy for solar X-ray flare predictions.

Inhibition of Sphingolipid Metabolism Enhances Resveratrol Chemotherapy in Human Gastric Cancer Cells

  • Shin, Kyong-Oh;Park, Nam-Young;Seo, Cho-Hee;Hong, Seon-Pyo;Oh, Ki-Wan;Hong, Jin-Tae;Han, Sang-Kil;Lee, Yong-Moon
    • Biomolecules & Therapeutics
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    • v.20 no.5
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    • pp.470-476
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    • 2012
  • Resveratrol, a chemopreventive agent, is rapidly metabolized in the intestine and liver via glucuronidation. Thus, the pharmacokinetics of resveratrol limits its efficacy. To improve efficacy, the activity of resveratrol was investigated in the context of sphingolipid metabolism in human gastric cancer cells. Diverse sphingolipid metabolites, including dihydroceramides (DHCer), were tested for their ability to induce resveratrol cytotoxicity. Exposure to resveratrol ($100{\mu}M$) for 24 hr induced cell death and cell cycle arrest in gastric cancer cells. Exposure to the combination of resveratrol and dimethylsphingosine (DMS) increased cytotoxicity, demonstrating that sphingolipid metabolites intensify resveratrol activity. Specifically, DHCer accumulated in a resveratrol concentration-dependent manner in SNU-1 and HT-29 cells, but not in SNU-668 cells. LC-MS/MS analysis showed that specific DHCer species containing C24:0, C16:0, C24:1, and C22:0 fatty acids chain were increased by up to 30-fold by resveratrol, indicating that resveratrol may partially inhibit DHCer desaturase. Indeed, resveratrol mildly inhibited DHCer desaturase activity compared to the specific inhibitor GT-11 or to retinamide (4-HPR); however, in SNU-1 cells resveratrol alone exhibited a typical cell cycle arrest pattern, which GT-11 did not alter, indicating that inhibition of DHCer desaturase is not essential to the cytotoxicity induced by the combination of resveratrol and sphingolipid metabolites. Resveratrol-induced p53 expression strongly correlated with the enhancement of cytotoxicity observed upon combination of resveratrol with DMS or 4-HPR. Taken together, these results show that DHCer accumulation is a novel lipid biomarker of resveratrol-induced cytotoxicity in human gastric cancer cells.

THE ANALYSIS OF INITIAL APICAL FILE SIZE BEFORE AND AFTER CORONAL FLARING (Coronal flaring 전, 후 초기근관장 파일크기의 분석)

  • Hwang, Ho-Keel;Park, Chan-Ho;Bae, Seong-Chul
    • Restorative Dentistry and Endodontics
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    • v.28 no.1
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    • pp.64-71
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    • 2003
  • The purpose of this study was to compare the initial apical file(IAF) first Ole that fits to the apex in each canal before and after early flaring to analyze if the size of file to fit to the apex would increase after flaring. Eighty anterior teeth with complete apical formation and patent foramens were selected. The samples were randomly divided into 4 groups(GG, OS, GT, PT Group) of 20 teeth each. A file was fit to the apex in each canal and that size recorded. Radicular flaring were completed using different types of instruments. After flaring a file was again fit to the apex in the same manner as before and its size recorded. The results of this study were as follows : 1. The mean diameter of IAF before flaring(file diameters in $mm{\times}10^{-2}$) was $19.81{\pm}8.32$ before and $25.94{\pm}9.21$ after(p<0.05). 2. The increase in diameter of IAF was approximately one file size for all groups. 3. Ranking of increasing diameter of IAF were GG>CT>OS>PT group. There was a statistically significant difference between before and after flaring(p<0.05). 4 Ranking of the time for flaring were GG>GT>OS>PT group. There was a statistically significant difference between GG group and other groups(p<0.05). 5. In the case without change of IAF diameter, they showed decrease in force after flaring when IAF was pulled out from root canal(p<0.05). This study suggested that early radicular flaring increases the file size that is snug at the apex, and awareness of that difference gives the clinician a better sense of canal size. Early flaring of the canal provides better apical size information and with this awareness, a better decision can be made concerning the appropriate final diameter needed for complete apical shaping.

Estimation of unused forest biomass potential resource amount in Korea

  • Sangho Yun;Sung-Min Choi;Joon-Woo Lee;Sung-Min Park
    • Korean Journal of Agricultural Science
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    • v.49 no.2
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    • pp.317-330
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    • 2022
  • Recently, the policy regarding climate change in Korea and overseas has been to promote the utilization of forest biomass to achieve net zero emissions. In addition, with the implementation of the unused forest biomass system in 2018, the size of the Korean market for manufacturing wood pellets and wood chips using unused forest biomass is rapidly expanding. Therefore, it is necessary to estimate the total amount of unused forest biomass that can be used as an energy source and to identify the capacity that can be continuously produced annually. In this study, we estimated the actual forest area that can be produced of logging residue and the potential amount of unused forest biomass resources based on GT (green ton). Using a forest functions classification map (1 : 25,000), 5th digital forest type map (1 : 25,000), and digital elevation model (DEM), the forest area with a slope of 30° or less and mountain ridges of 70% or less was estimated based on production forest and IV age class or more. The total forest area where unused forest biomass can be produced was estimated to be 1,453,047 ha. Based on GT, the total amount of unused forest biomass potential resources in Korea was estimated to be 117,741,436 tons. By forest type, coniferous forests were estimated to be 48,513,580 tons (41.2%), broad-leaved forests 27,419,391 tons (23.3%), and mixed forests 41,808,465 tons (35.5%). Data from this research analysis can be used as basic data to estimate commercial use of unused forest biomass.

Effects of Herbal and Western Medicines on Liver Functions (한.양약 복용이 간기능이상에 미치는 영향에 대한 연구)

  • Park, Hae-Mo;Shin, Heon-Tae;Lee, Sun-Dong
    • Journal of Society of Preventive Korean Medicine
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    • v.11 no.2
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    • pp.23-39
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    • 2007
  • Objectives : The aim of this study was to evaluate effects of herbal medicine and other associated factors for abnormal liver function tests(especially total bilirubin, AST, ALT, and ${\gamma}GT$) levels in outpatients at an oriental medical clinic. Methods : A cross-sectional study based on clinical records was conducted on 1,871 patients at one Oriental medical clinic in Seoul, Korea. 504 patients received a liver function tests for screening and 497 patients ranging from the age of 4 to 74 were chosen for the study. Patients with basal liver disease or DM were excluded during the screening process. Patients were classified into case(abnormal) and control(normal) groups by normal liver function test references. Results and Conclusions : In this study, 33.0% of the patients were females, and 67.0% were males. The mean age was $34.7{\pm}11.9$ years old. The mean total protein value was $6.78{\pm}0.66g/dL$(male $6.79{\pm}0.61$, female $6.76{\pm}0.76$), albumin $3.89{\pm}0.50g/dL$(male $3.94{\pm}0.47$, female $3.81{\pm}0.54$), total bilirubin $0.51{\pm}0.35mg/dL$(male $0.54{\pm}0.39$, female $0.45{\pm}0.23$), AST $23.31{\pm}18.22U/L$(male $26.37{\pm}20.73$, female $17.09{\pm}8.72$), ALT $33.49{\pm}36.36U/L$(male $40.56{\pm}41.77$, female $19.13{\pm}12.64$), LDH $258.07{\pm}74.84mg/dL$(male $263.68{\pm}73.77$, female $246.70{\pm}75.92$), and ${\gamma}GT$ $39.64{\pm}59.16U/L$(male $50.15{\pm}69.43$, female $17.83{\pm}8.36$). The percentage of abnormal total bilirubin levels(>1.0) in these patients was 2.6%, abnormal AST levels(>39m, >29f) 8.5%, ALT levels(>47m, >32f) 18.8%, and ${\gamma}GT$ (>50m, >40f) 19.9%.

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A Study on the Proper Crown Height of GT 100,000Ton Cruise ship and DWT 100,000Ton Container ship (10만톤급 크루즈선과 컨테이너선의 적정 마루높이에 관한 연구)

  • Kim, Seungyeon;Lee, Yunsok
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.24 no.2
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    • pp.157-162
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    • 2018
  • The increase of risk in port due to the increase in ship size and sea level rises, the standard crown height will increase. In this study, cruise and container ships will need to raise their crown height due to the projected wind pressure areas becoming larger due to the ships' size increase. The mooring assessment was evaluated with the rise of the crown height. The cruise ship of GT 100,000 tons exceeded the permissible breaking force of the mooring line under the crown height conditions of wind speed of 30 kts when the wind direction was $45^{\circ}$ to the direction of the bow. Also, the elevation angle of the pier and mooring line was analyzed and exceeded the crown height, and it was determined that it is necessary to adjust the crown height. Container ships of DWT 100,000 tons were analyzed to exceed the limit of sway motion at the crown height and it was determined that they need to be adjusted to the minimum crown height standard.

Effects of herbal acupuncture(Atratylodes japonica, Coix lachrymajobi, Ephedra sinica, Atratylodes japonica mixed with Coix lachrymajobi and Ephedra sinica mixed with Green tea) at Pungnyung(ST40) and Umnungchon(SP9) in obese Rats induced by high fat diet (고지방식이(高脂肪食餌)로 유발(誘發)된 비만백서(肥滿白鼠)모델에서 창출(蒼朮), 의이인(薏苡仁), 마황(麻黃), 창출합의이인(蒼朮合薏苡仁) 마황합록차(麻黃合綠茶) 약침(藥鍼)이 혈청지질(血淸脂質) 및 간기능(肝機能)에 미치는 영향(影響))

  • Youn, Dae-Hwan;Kang, Ja-Don;Joo, Joon-Sung;Chae, Woo-Seok;Na, Chang-Su
    • Journal of Pharmacopuncture
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    • v.7 no.1 s.12
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    • pp.87-100
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    • 2004
  • Objective : This research was performed to investigate the effect of herbal acupuncture(Atratylodes japonica, Coix lachrymajobi, Ephedra sinica, Atratylodes japonica mixed with Coix lachrymajobi and Ephedra sinica mixed with Green tea) at Pungnyung(ST40) and Umnungchon(SP9) on weight gain, food intake, food efficiency, serum of lipid concentrations, liver function and HDL to total cholesterol ratio of rats fed high fat diet for 5weeks. Method : Experimental groups were divided into normal group(Normal), high fat diet group(Control), high fat diet and Atractylodes japonica-herbal acupuncture group(AJ), high fat diet and Coix lachrymajobi-herbal acupuncture group(CL), high fat diet and Ephedra sinica-herbaI acupuncture group(ES), high fat diet and Atractylodes japonica+Coix lachrymajobi-herbal acupuncture group(AJ+CL), Ephedra sinica+Green tea-herbal acupuncture group(ES+GT). Herbal acupuncture was bilaterally treated at the level of 132.5mg/kg body weight per 2day. Results : Body weight and food efficiency were decreased in AJ, ES, AJ+CL, ES+GT. The level of serum total cholesterol, triglyceride and free fatty acid were increased in AJ, ES, ES+GT. That of serum HDL-cholesterol was increased in AJ. The change of food intake, the level of serum phospholipid and ALP were not significant. The HDL to Total cholesterol ratio was increased in AJ and ES. Conclusion : Atractylodes japonica-herbal acupuncture in ST40 SP9 is effective on Body weight, food efficiency ratio, the level of serum lipid, protection of liver function and prevention cardiovascular risk by obesity induced by high fat diet. Herbal acupuncture mixed Ephedra sinica with Green tea can control the body weight, food efficiency ratio and the level of serum lipid.

Anticarcinogenic Effects of Extracts from Gloiopeltis tenax (참가사리 분획물의 암 예방효과)

  • Jung, Young-Hwa;Jung, Bok-Mi;Shin, Mi-Ok;Bae, Song-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.4
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    • pp.395-401
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    • 2006
  • In this study, we investigated anticarcinogenic effects of extracts from Gloiopeltis tenax (GT). GT was extracted with methanol (GTM), which was then further fractionated into four fractions by using solvent fractionation method, affording methanol (GTMM), hexane (GTMH), butanol (GTMB) and aqueous (GTMA) soluble fractions. We determined the cytotoxic effects of these fractions on cancer cells by MTT assay. Among various fractions of GT, the GTMM showed the strongest cytotoxic effect at concentration of $150{\mu}g/mL$, displaying 95.97% on HepG2 cell lines and 93.64% on HT-29 cell lines, respectively. And, the anti-proliferative effect of GT was accompanied by a marked in increase of levels of Bad, Bax, Bok and Bak protein and activation of caspase-3, caspase-7 and PARP protein. Also, we observed quinone reductase (QR) induced effects in all fraction layers of GT on HepG2 cells. The QR induced effects of the GTMM and GTMB on HepG2 cells at concentration of $60{\mu}g/mL$ showing inductive indexes of 2.86 and 2.04 compared to the control value of 1.0.

A Novel Phototransduction Pathway in the Pineal Gland and Retina

  • Okano, Toshiyuki;Kasahara, Takaoki;Fukada, Yoshitaka
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.246-248
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    • 2002
  • Light is a major environmental signal for entrainment of the circadian clock, but little is known about the phototransduction pathway triggered by light-activation of photoreceptive molecule(s) responsible for the phase shift of the clock in vertebrates. The chicken pineal gland and retina contain the autonomous circadian oscillators together with the photic entrainment pathway, and hence they provide useful experimental model for the clock system. We previously demonstrated the expression and light-dependent activation of rod-type transducin $\alpha$-subunit (Gtl$\alpha$) in the chicken pineal gland. It is unlikely, however, that the pineal Gt$_1$$\alpha$ plays a major role in the photic entrainment, because the light-induced phase shift is unaffected by bloking the signaling function of Gt$_1$$\alpha$. Here, we show the expression of G 11 $\alpha$, an $\alpha$-subunit of another heterotrimeric G-protein, in the chicken pineal gland and retina by cDNA cloning, Northern blot and Western blot analyses. GIl$\alpha$-immunoreactivity was colocalized with pinopsin in the chicken pineal cells and it was found predominantly at the outer segments of photoreceptor cells in the retinal sections, suggesting functional coupling of G11 $\alpha$ with opsins in the both the tissues. By coimmunoprecipitation experiments using the retina, we showed the light- and GTP-dependent interaction between rhodopsin and G11 $\alpha$. Upon ectopic expression of a Gq/ 11-coupled receptor in cultured pineal cells, pharmacological (non-photic) activation of endogenous G11 induced phase-dependent phase shifts of the melatonin rhythm in a manner very similar to the effect of light. These results suggested opsin-G11 pathway contributing to the photic entrainment of the circadian clock.

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