• Title/Summary/Keyword: $F_1$ hybrids

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Progeny Analysis of Hybrid Lilies Crossed Between Several Genotypes and Tetraploid Oriental-Asiatic Hybrids (체세포 염색체가 배가된 4배체 Oriental-Asiatic(OA) hybrid 유래 종간잡종 백합의 후대검정)

  • Chung, Mi-Young;Chung, Jae-Dong;Van Tuyl, Jaap M.;Lim, Ki-Byung
    • Korean Journal of Breeding Science
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    • v.42 no.1
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    • pp.100-108
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    • 2010
  • This study was carried out to confirm the chromosome constitution and homoeologous recombination of progenies derived from various cross combination using tetraploid OA interspecific hybrid originated from mitotic chromosome doubling. Based on the chromosome analysis of progenies crossed reciprocally, there were only triploid progenies when crossed with diploid Asiatics as male or female parent. While only tetraploid progenies were produced when crossed tetraploid Asiatics or tetraploid OA hybrid with tetraploid OA hybrid, respectively. However, two types of progenies, that is, diploid and triploid plants, were produced from cross combinations between diploid Oriental hybrid and tetraploid OA hybrid. From the GISH analysis of OA hybrid, it was confirmed that diploid $F_1$ OA hybrid was consisted of 24 chromosomes (12 Oriental and 12 Asiatics) showing authentic OA hybrid. On the other hand, it was notified that triploid plants (3x=36) were consisted of 24 Asiatics lily chromosomes and 12 Oriental lily chromosomes by analysis of backcross progenies derived from either $A{\times}OA$ or $OA{\times}A$ crosses. In cross between tetraploid OA and OA, all the progenies were tetraploid with equal number of chromosomes without any homoeologous recombination, i.e. each 24 chromosomes of Oriental and Asiatics. In 2x-4x ($O{\times}OA$) cross combination, some progenies had 2x=24 chromosomes originated from only Oriental hybrid, and other progenies had 3x=36 chromosomes derived from 24 chromosomes of Oriental hybrid and 12 chromosomes of Asiatic hybrid. Only tetraploid Asiatics chromosomes without any Oriental one were produced in all the progenies from 4x-4x ($AA{\times}OA$) cross combination.

Changes in Variety and Cultural Practices of Soybean, Sweet Potato and Corn Since 1962 in Korea (하전작물 품종 및 재배기술의 1962년 이후 변천)

  • Keun-Yong Park;Eun-Hui Hong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.27 no.4
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    • pp.462-469
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    • 1982
  • Since 1962, varietal development and dissemination for summer upland crops have been actively initiated by the Crop Experiment Station, Office of Rural Development, Suweon, Korea. The major breeding objectives of soybeans have been to develop varieties which are early maturing, disease resistant, and adaptable to late planting for after-barley cropping. Development of eleven new soybean varieties including Hwangkeumkong, Jangyeobkong, Danyeobkong, and Kwangkyo has greatly increased the soybean yield throughout country. For com, after development of Hwangok #2, a synthetic, in early 1960's, nine corn hybrids-single crosses, double-crosses, and three-way crosses-such as Jecheon-ok, Hoengseong-ok, Kwangok, Suweon #19, etc., have been disseminated mainly to Kangweon province, a major corn producing area in Korea, and drew up the yield over 4 tons per hectare. The major breeding objectives of sweet potato have been to develop varieties which have high starch content and root yield. Hwangmi, Hongmi, and Shinmi are three sweet potato varieties developed and disseminated by the Crop Experiment Station, Office of Rural Development and are grown most widely in Korea. Most of researches on cultural practices of upland crops have begun on a full scale from early 1960's. In soybeans, for example, no fertilizer but for barley was applied although the effects of phosphate and potassium fertilizers were great on soybeans in after-barley soybean croppings. The effects of heavy application of phosphate and calcium fertilizers on soybeans in newly reclaimed soils were recognized. Recently a mixed fertilizer for soybean (N; 40, P:70, K:60 kg/㏊) was developed and sold for soybean growers. The optimum planting densities of 220, 000 plants/ha in full-season cropping and 330, 000 pts/ha in after-barley cropping of soybeans were known from repeated experiments. For higher yield, a means of cultural practices such as transplanting-pinching, direct planting-pinching, and hilling-up, etc., were developed along with barley-stubble planting with no tillage and integrated herbicide application for labour savings. For sweet potato, cultural practices for planting date, harvesting date, fertilizer, and planting density were fully established. For early marketing, a technique of vinyl-mulching on sweet potato has also fully developed. For com, planting density of 37, 000 pts/ha in early 1960's has been changed to 55, 000 pts/ha for grain production and 67, 000 pts/ha for silage. The amounts of fertilizers have also been changed from 120-120-120kg/ha (N-P-K) in early 1960s to 180-150-150 kg/ha. These increases in number of plants per unit area and fertilizer levels have resulted in greater production for both grain and silage. At the same time, the production techniques of F1 seeds have also improved.

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Utilization of Male Sterility Type and Frequency of Male Sterile Genes in Carrots (당근 웅성부임성(雄性不稔性)의 형태별(形態別) 이용현황(利用現況)과 핵내(核內) 웅성부임(雄性不稔) 유전자(遺傳子)의 분포(分布))

  • Suh, Young Ki;Youn, Goang Hyeon;Cho, Young Hwan;Paek, Kee Yoeop
    • Horticultural Science & Technology
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    • v.17 no.4
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    • pp.470-472
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    • 1999
  • When the type and ratio of male-sterile plants in $F_1$ hybrids collected from several seed companies were investigated, there were differences in the male-sterile type depending upon region, seed company and variety group. The differences were inferred due to the easiness in breeding of maintainer line among the variety groups. American seed companies mainly used petaloid type male sterility with Imperater group varieties. European companies used brown anther type male sterility mainly with Nantes group but some companies used petaloid type also in varieties that were different from Nantes group. Asian companies (Japan, Korea) used both types with Chantaney and Kuroda group varieties, but one type was mainly used depending on individual seed company. Only one type of male-sterility in one variety was observed and the results were agreed well with other's results that male-sterility type was determined by cytoplasm factor. Some breeding lines were backcrossed to both types of cytoplasm (Sa, Sp) for maintainer line selection. We could select 15 maintainer lines from 20 lines in petaloid cytoplasm (Sp) and 3 from 4 lines in brown cytoplasm (Sa). In petaloid cytoplasm, maintainer lines can be selected at considerably high frequency. But in brown anther cytoplasm, the used materials are too restricted to tell general frequency maintainer lines.

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Growth, Photosynthesis and Rubisco Activity of Resistant Hybrid Poplar(Populus trichocarpa×P. deltoides) to Ozone Exposure: A Link with Compensatory Strategy (오존에 노출(露出)시켰을 때 저항성(抵抗性)을 갖는 잡종(雜種)포플러의 생장(生長), 광합성(光合成) 그리고 Rubisco 활성(活性)에 관(關)한 연구(硏究): 수목(樹木)의 보상전략(補償戰略)과의 관계(關係))

  • Woo, Su-Young
    • Journal of Korean Society of Forest Science
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    • v.86 no.1
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    • pp.80-86
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    • 1997
  • The objective of this study was to investigate how resistant poplar hybrid makes compensation to ozone stress. Growth, net assimilation rate and initial Rubisco activity were investigated. This study elucidates the physiological mechanisms associated with ozone sensitivity and resistance in 3 selected $F_2$ hybrids, a family originating from a cross between Populus trichocarpa${\times}$P. deltoides. Open-top chambers were used. Ozone concentrations varied from 90 to 115 ppb for 126 days, 6 to 9 hours in a day. This study tested the hypothesis that resistant poplar hybrid maintains the biomass production to ozone exposure via increased net assimilation rate and Rubisco activity. Growth, biomass, net assimilation rate and initial Rubisco activity were generally reduced by ozone treatment. In the tree parts, root under ozone stress was the most sensitive part. Reduced allocation of photosynthates to root growth might be due to increased respiratory demands for maintenance and repair of aboveground tissue damaged by ozone stress. Maintenance or increases remaining leaves in photosynthetic rates and Rubisco activity in resistant clone in response to ozone treatment were the results of biological compensation to ozone stress.

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The Maize with Multiple Ears and Tillers (MET) III. Developmental Habit and Morphology of the Tillers (다얼성 옥수수 연구 III. 분얼발생의 습성 및 형태)

  • Choe, Bong-Bo;Lee, Hee-Bong;Lee, Won-Koo;Kang, Kwon-Kyoo;Jong, Seung-Keun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.1
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    • pp.23-29
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    • 1989
  • In order to investigate developmental habit and morphology of maize tillers, time and location of tiller development. number of tillers per plant, tiller angle, height and diameter of tillers and root systems of tillers were examined under field condition for maize with tillers. Materials used were mostly from Korean local lines and a few lines from other countries were also included for comparison. The time of the first tiller development was about 18 to 20 days after emergence when planted on May in Yusong. The second tiller appeared about 4 to 5 days after the first tiller appeared. The tiller number per plant varied with lines and hybrids and ranged from two to ten. The location of tiller development was usually basal nodes of the main stem. Each tiller appeared to have its own root system. The angle between tillers and main stem was variable depending upon the maizes and the tiller angle could be classified into three categories. The height of tillers was also variable and seemed to be under genetic control. The most productive tillers were found among the Korean local derivatives.

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Construction of Genetic Linkage Map and Identification of Quantitative Trait Loci in Populus davidiana using Genotyping-by-sequencing (Genotyping-by-sequencing 기법을 이용한 사시나무(Populus davidiana) 유전연관지도 작성 및 양적형질 유전자좌 탐색)

  • Suvi Kim;Yang-gil Kim;Dayoung Lee;Hye-jin Lee;Kyu-Suk Kang
    • Journal of Korean Society of Forest Science
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    • v.112 no.1
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    • pp.40-56
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    • 2023
  • Tree species within the Populus genus grow rapidly and have an excellent capacity to absorb carbon, conferring substantial ability to effective purify the environment. Poplar breeding can be achieved rapidly and efficiently if a genetic linkage map is constructed and quantitative trait loci (QTLs) are identified. Here, a high-density genetic linkage map was constructed for the control pollinated progeny using the genotyping-by-sequencing (GBS) technique, which is a next-generation sequencing method. A search was also performed for the genes associated with quantitative traits located in the genetic linkage map by examining the variables of height and diameter at root collar, and resilience to insect damage. The height and diameter at root collar were measured directly, while the ability to recover from insect damage was scored in a 4-year-old breeding population of aspen hybrids (Odae19 × Bonghyeon4 F1) established in the research forest of Seoul National University. After DNA extraction, paternity was confirmed using five microsatellite markers, and only the individuals for which paternity was confirmed were used for the analysis. The DNA was cut using restriction enzymes and the obtained DNA fragments were prepared using a GBS library and sequenced. The analyzed results were sorted using Populus trichocarpa as a reference genome. Overall, 58,040 aligned single-nucleotide polymorphism (SNP) markers were identified, 17,755 of which were used for mapping genetic linkages. The genetic linkage map was divided into 19 linkage groups, with a total length of 2,129.54 cM. The analysis failed to identify any growth-related QTLs, but a gene assumed to be related to recovery from insect damage was identified on linkage group (chromosome) 4 through genome-wide association study.

Tolerance of Corn, Sorghum, Sorghum-Sudangrass Hybrid, and Pearl Millet to Simazine and Alachlor (옥수수, 수수, 수수-수단그라스 교잡종 및 진주조의 Simazine과 Alachlor에 대한 저항성)

  • 이석순;최상집
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.2
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    • pp.113-119
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    • 1989
  • In 1988 the tolerance of corn, sorghum, sorghum-sudangrass. and pearl millet hybrids to simazine and alachlor was tested in field during the growing season and pots during the summer and fall. In field and summer pot experiments(Exp.) the above mentioned four crops were tested at the ratios of simazine WP (50% ai, g/10a) ; alachlor EC (43.7% ai. ml/10a) of 130: 0, 100: 0, 70: 200, 0: 300 and 0: 400 and a sorghum hybrid was tested at 0, 50, 100, 200, 300, and 400ml/10a of alachlor and 70g/10a of simazine+ 200ml/10a of alachlor in fall pot Exp. In corn emergence rate, percent stand, plant height of seedlings, and dry matter(DM) yield were not affected by simazine and alachlor in all Exps. In sorghum and sorghum-sudangrass early growth and DM yield were not affected by simazine and alachlor in field Exp. In contrast, simazine reduced height and dry weight of seedlings slightly without any deterimental effects on emergence and survival rates. but alachlor reduced survival rate, plant height, and dry weight of seedlings significantly in summer pot Exp. In fall Exp. alachlor did not affect emergence rate of a sorghum hybrid, but survival rate, plant height, and dry weight of seedlings reduced with increased levels of alachlor when applied higher than 100ml/10a. In pearl millet simazine did not affect emergence rate, plant height, and DM yield in field, but reduced survival rate, plant height, and dry weight of seedlings in summer pot Exp. However, alachlor reduced DM yield significantly due to a lower percent stand even in the field. In summer pot Exp. although emeregence rate was slightly reduced, all seedlings were dead after emergence. Simazine did not control grasses such as Digitaria sanguinalis, Setaria viridis, Echinochloa crusgalli effectively, but controlled broadleaf weeds. Alachlor controlled all grasses, Porluraca oleracea, and Amaranthus mangostanus, but did not control Acalypha australis and Chenopodium album. A combination of simazine and alachlor controlled weeds more effectively than either simazine or alachlor alone.

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Studies on the Virus in Silkworm Bombyx mori L. -Resistance to Virus Disease- (가잠 Virus에 관한 연구 -저항성에 관한 기초조사-)

  • 박광의;강석권
    • Journal of Sericultural and Entomological Science
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    • v.9
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    • pp.67-87
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    • 1969
  • 1. Objectives and Importance. Many silkworms have been damaged by nuclear polyhedrosis virus diseases thoughout the country every year causing a decease in cocoon production by approximately ten per cent per year. The damage caused by the infections virus has occured in spite of complete disinfection. In this respect, it is well known it is impossible, at the present time, to protect the silkworm from these virus infections through chemical and physical control methods. Therefore, this author has attempted to solve this urgent problem from the view point of heredity and breeding, discovering the different resistances and heritabilities among 120 stains collected from throughout the country, and selecting the ones with highest resistance for the basic materials in the silkworm breeding. 2. Results of work 1) The strains with strong resistance to the nuclear polyhedrosis virus diseases are N$_4$, N$\sub$15/, N$\sub$48/, C$\sub$55/ and Em. the log ED$\sub$50/ values of them vary between 0.799 and 1.611. The susceptible strains are N$\sub$20/, C$\sub$62/, N$\sub$76/, N$\sub$79/ and C$\sub$108/, the log ED$\sub$50/ values of them vary between 5.159 and 7.258. (Reference Table 4) The Japanese strain with a log ED$\sub$50/, value of 3.770 is the strongest, followed by the Chinese strain with a log ED$\sub$50/ value of 3.564. The weakest is the European strain with a log ED$\sub$50/ value of 3.3381. The direction coefficient of the regression equation of the susceptibility varies between 0.1 and 0.6, the uniformness of the resistance of the preserved strains of this country is comparatively low. The hereditary henomena of the resistance of each strain and the conerete method of its application for silkworm breeding main the subjects for later studies. 2) The content of water and ash in silkworm has not been correlated with the capability for resistance to the virus diseases(Reference. Table 8). but it is very significantly correlated with mortality rate (in common reaning). In the case of the silkworms which have just completed the fourth moulting the content of water and ash is not related to the mortality rate. In the case of the silkworms which have just completed the third moulting, however, the water( +0.326) and ash (+0.362) registered a high significance. The ash content in the first ($\div$0.520) and second ($\div$0.386) moults is highly significant but water content in both cases is not significant (Reference Table 7). 3) The No. 205 strain proved to be the best in character among the various F$_1$ hybrids. No. 204 was very good in strength but a little lower in cocoon character than the control. No. 212 was a little low in coccon character and mortality was average, but the cocoon harvest was the best among all the varieties offered for (Reference Table 9). 4), In short, the above mentioned strains which are known to have strong resistance to the virus disease are expected to provide basic data for breeding strong varieties. It is proposed that continued research should be conducted on the characteristics of various strains for a satisfactory preservation of various characteristics.research should be conducted on the characteristics of various strains for a satisfactory preservation of various characteristics.

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