• Speech Sciences
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• v.9 no.4
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• pp.15-25
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• 2002

It's not easy to produce very high tones during singing for not only untrained ordinary people but also even trained singers. To get high singing tones from the low tones, some trained singers used to use a distinguished singing technique, Passaggio (vocal register transition). The purpose of this study is to compare several voice parameters variation between when to sing with using the passaggio technique and to sing without using it. We selected 18 male singers (tenor 8, baritone 10), who had more than 7 years of experience and were well trained in passaggio technique. Simultaneous measurements of fundamental frequency (F0), mean flow rate (MFR), intensity (I), and subglottal pressure (Psub) were performed using the phonatory function analyzer (Nagashima). For the tenor, target tones /a/ were presented: 1) easy phonation: $B_{2}$, 2) high tone without passaggio: F$#_{3}$ 3) high tone with passaggio: F$#_{3}$. For the baritone, target tones /a/ were presented: 1) easy phonation: G$#_{3}$, 2) high tone without passaggio: D$#_{3}$, 3) high tone with passaggio: D$#_{3}$. F0 of the target tones between non-passaggio group and passaggio group was almost the same in both tenor and baritone groups. Intensity of the non-passaggio and passaggio vocalization was much louder than that of easy phonation and pasaggio was louder than non-passaggio vocalization (especially statistically significant in baritone singers). MFR of the passaggio vocalization was greater than non-passaggio vocalization in both tenor and baritone group, but statistically significant only in baritone. Psub of the passaggio vocalization was greater than that of the non-passaggio vocalization in both tenor and baritone group, but statistically not significant in tenor.

• Phonetics and Speech Sciences
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• v.4 no.4
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• pp.139-146
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• 2012

This study was designed to examine comfort levels of pitch and loudness with target matching and their effects on electroglottographic (EGG) and acoustic measures. Twelve speakers, six males and six females, were instructed to produce /a/ sustained vowel for three seconds at a comfortable pitch and loudness level without any instruction and with a target matching procedure of either a certain f0 or SPL separately with visual and auditory feedback. The range of pitch for females and males were presented by progressing up and down randomly at intervals of 5Hz from 150 Hz to 310 Hz (total 33 frequency targets) and from 85 Hz to 190 Hz (total 22 frequency targets), respectively. The loudness levels were 65, 75, 85, 95 dB (total of four intensity targets) for both males and females. Subjective estimations of comfortable levels were obtained using a 10-point equal-appearing interval rating scale following each phonation. The results showed that males and females demonstrated similar trends in loudness levels with greatest comfort at 75 dB, whereas pitch comfort ratings showed a greater variability with females having a wider range with target matching. In the comfort levels of individuals, most male and female speakers rated higher comfort at soft, rather than loud phonations. On the other hand, most male speakers perceived highest comfort levels below the comfort pitch levels they phonated under natural conditions. Higher frequency ranges, however, were perceived to be more comfortable than those of natural condition in most female speakers, although the comfortable pitch levels in spontaneous phonations were within the comfort level ranges determined by targeted phonations. When comparing acoustic (%jitter, %shimmer, SNR) and EGG measures (CQ%) between spontaneous comfortable phonations and targeted phonations produced by the same subject at similar f0 and intensity, no significant differences were observed (p>0.05). Thus, target matching procedures may be considered a compatible and alternative method to reduce the variability of comfortable pitch and loudness levels by eliciting consistent comfortable phonations.

• Development and Reproduction
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• v.23 no.3
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• pp.277-284
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• 2019

ARID1A and PGR plays an important role in embryo implantation and decidualization during early pregnancy. Uterine specific Arid1a knockout ($Pgr^{cre/+}Arid1a^{f/f}$) mice exhibit in non-receptive endometrium at day 3.5 of gestation (GD 3.5). In previous studies, using transcriptomic analysis in the uterus of $Pgr^{cre/+}Arid1a^{f/f}$ mice, we identified proline-rich acidic protein 1 (PRAP1) as one of the down-regulated genes by ARID1A in the uterus. In the present study, we performed RT-qPCR and immunohistochemistry analysis to investigate the regulation of PRAP1 by ARID1A and determine expression patterns of PRAP1 in the uterus during early pregnancy. During early pregnancy, PRAP1 expression was strong at day 0.5 of gestation (GD 0.5) and then decreased at GD 3.5 in the epithelium and stroma. After implantation, PRAP1 expression was remarkably reduced in the uterus. However, the expression of PRAP1 at GD 3.5 was remarkably increased in the $Pgr^{cre/+}Arid1a^{f/f}$ mice. To determine the ovarian steroid hormone regulation of PRAP1, we examined the expression of PRAP1 in ovariectomized control, $Pgr^{cre/+}Arid1a^{f/f}$, and progesterone receptor knock-out (PRKO) mice treated with progesterone. While PRAP1 proteins were strongly expressed in the luminal and glandular epithelium of control mice treated with vehicle, progesterone treatment suppressed the expression of PRAP1. However, PRAP1 was not suppressed in both the $Pgr^{cre/+}Arid1a^{f/f}$ and PRKO mice compared to controls. Our results identified PRAP1 as a novel target of ARID1A and PGR in the murine uterus.

• Journal of the Korean Ceramic Society
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• v.39 no.8
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• pp.742-749
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• 2002

The PZT thin films werre deposited on Pt/Ti/$SiO_2$/Si substrate by R. F. magnetron sputtering with $Pb_{1.1}Zr_{0.53}Ti_{0.47}O_3$ target. When interlayers(PbO, $TiO_2$, PbO/$TiO_2$) were inserted between PZT and Pt, the crystallization of the PZT thin films was considerably improved and the processing temperature was lowered. Compared to the pure PZT thin films, dielectric constant, dielectric loss and polarization properties of PZT thin films with interlayers were considerably improved. From XPS depth profile analysis, it was confirmed that PZT thin films and interlayers existed independently. In particular, PZT thin films deposited on interlayer(PbO/$TiO_2$) showed the best dielectric property (${\varepsilon}_r$=414.94, tan${\delta}$=0.0241, Pr=22${\mu}C/cm^2$).

• The Journal of Korean Society for Radiation Therapy
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• v.29 no.2
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• pp.53-64
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• 2017

Purpose: To evaluate the usefulness of the $HexaPOD^{TM}$ evo RT system(6DoF couch) and the tendency of dose difference according to size of rotational direction error for volumetric rotational modulated radiotherapy(V-MAT) in patients with long target lengths. Therefore, it is suggested to recommend the need for rotational error correction. Materials and Methods: Ten patients with Esophagus cancer or Breast cancer including SCL treated with HexaPOD 6DoF(Six-Degree of Freedom) couch were included in this study. 6DoF couch was used to measure the difference in dose according to the rotation error in the directions of Rx(pitch), Ry(roll), and Rz(yaw). Each rotation error was applied. Positioning variation on x, y and z axis was verified and random variations were made by 6DoF couch with positioning variation. Modified DQA is conducted and point dose and gamma value are analyzed and compared. In addition, after applying the rotation error every $1^{\circ}$ to treatment plans of each target with a diameter of 3 cm, 5, 10, 15, and 20 cm respectively, gamma passing rate is being monitored by its aspect of change according to types and sizes of the target length and rotation error. Results: Mean error of the point dose and Gamma passing rate when the position variation was applied were $2.50{\pm}1.11%$ and $84.1{\pm}7.39%$ in the Rx direction, $2.36{\pm}1.16%$, and $81.0{\pm}8.49%$ in the Ry, $2.35{\pm}1.10%$ and $84.4{\pm}6.99%$ in the Rz direction, respectively. As a result of analysis on gamma passing rate according to types and sizes of the target length and rotation error, the gamma passing rate tended to decrease with increasing rotation error in the Rx and Rz directions except Ry direction. In particular, the lowest gamma passing rate (74.2 %) was in the case of $2.5^{\circ}$ rotation error in Rz direction of the target of 10 cm. Conclusion: The correction of the rotational error is needed for volumetric modulated radiotherapy of the treatment area with a long target length, and the use of 6DoF couch will improve the reproducibility of the patient position and the quality of the treatment.

• Biodesign
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• v.6 no.4
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• pp.84-91
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• 2018

Spo0F is a response regulator that modulates sporulation, undergoes phosphorylation for phosphorelay signal transduction, and interacts with various regulatory proteins; however, the mechanisms through which phosphorylation induces structural changes and regulates interactions with binding partners remain unclear. Here, we determined the unphosphorylated crystal structure of Spo0F from the psychrophilic bacterium Paenisporosarcina sp. TG-14 (PaSpo0F) and established a phosphorylation-state structural model. We found that PaSpo0F underwent structural changes (Lys54 and Lys102) by phosphorylation and generated new interactions (Lys102/Gln10 and Lys54/Glu84) to stabilize the ${\beta}4/{\alpha}4$ and ${\beta}1/{\alpha}1$ loop structures, which are important target-protein binding sites. Analysis of Bacillus subtilis Spo0 variants revealed movement by BsSpo0F Thr82 and Tyr84 residues following interaction with BsSpo0B, providing insight into the movement of corresponding residues in PaSpo0F (Thr80 and Tyr82), with further analysis of BsSpo0F/BsRapH interaction revealing alterations in the ${\beta}4/{\alpha}4$ loop region. These results suggest that phosphorylation-induced structural rearrangement might be essential for PaSpo0F activation and expand the understanding of Spo0F-specific activation mechanisms during sporulation.

• Phonetics and Speech Sciences
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• v.3 no.2
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• pp.37-52
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• 2011

This study investigates patterns of the alignment of the accentual peaks in bisyllabic words of the CVNCV, CVNV, and CVNNV structures in North Kyungsang Korean. Based on the tonal alignment, patterns of the F0 pitch excursion are discussed relative to one another. Issues are addressed concerning how the tonal targets are aligned, and how the tonal specifications of nasals in postvocalic, intervocalic, and prevocalic environments are supplied in the LH, HL, and HH classes. Tonal specification of nasals in various environments is accounted for by extension of the L target, displacement of the pitch peak, and interpolation between two tonal targets, depending on the tonal class. The results in this study provide preliminary evidence that the categorical alignment of the tonal targets is implemented by simply checking the presence or absence of a nasal before or after the nucleus vowel on the segmental string, without reference to the constituency of the nasal in the syllable structure. However, the prosodic structure has a key role to play in explaining speaker-dependent variations in the tonal alignment. Sensitivity to tautosyllabicity has an effect on the shape of the F0 contour, and disparity in the patterns of the pitch excursion is represented as a function of syllable structure correlated with segmental composition of the nasal.

• Current Optics and Photonics
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• v.6 no.4
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• pp.420-429
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• 2022

In this paper, the details and design process of an optical system for space target detection cameras are introduced. The whole system is divided into three structures. The first structure is a short-focus visible light system for rough detection in a large field of view. The field of view is 2°, the effective focal length is 1,125 mm, and the F-number is 3.83. The second structure is a telephoto visible light system for precise detection in a small field of view. The field of view is 1°, the effective focal length is 2,300 mm, and the F-number is 7.67. The third structure is an infrared light detection system. The field of view is 2°, the effective focal length is 390 mm, and the F-number is 1.3. The visible long-focus narrow field of view and visible short-focus wide field of view are switched through a turning mirror. Design results show that the modulation transfer functions of the three structures of the system are close to the diffraction limit. It can further be seen that the short-focus wide-field-of-view distortion is controlled within 0.1%, the long-focus narrow-field-of-view distortion within 0.5%, and the infrared subsystem distortion within 0.2%. The imaging effect is good and the purpose of the design is achieved.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.05a
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• pp.112-112
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• 2004

양전자 단층촬영(PETㆍ Positron Emission Tomography)에 가장 널리 사용되는 방사성의약품인 FDG는 방사성동위원소인$^{18}$ F가 사용되며, 이는 안정물질인 H$_2$$^{18}$ O을 액체표적에 주입한 후, 고 에너지의 양성자빔을 조사하여 생산한다. 표적은 내화학성 및 높은 인장강도론 가진 재질인 titanium으로 제조하며, 0.075mm의 얇은 박판이 양성자빔 입사부에 사용된다. H$_2$$^{18}$ O가 주입된 표적에 양성자빔이 입사되는 순간 표적 내부는 높은 에너지로 인하여 표적물은 고온상태로 기화가 일어나고 이것이 압력을 증가시켜 target window는 바깥쪽으로 팽창한다.(중략)

• Speech Sciences
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• v.8 no.1
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• pp.21-31
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• 2001

The effects of focus on prosodic phrasing, F0, and duration are investigated paying attention not only to the target of focus but also to the constituents that are outside the domain of focus in Korean. We find that the constituents preceding and following the focused word tend to be dephrased. Dephrasing does not always cover up to the Intonation Phrase boundary contrary to Jun's (1993) claim. Dephrasing caused by focus determines F0 and durational difference between focused and neutral sentences. Syntactic constituency is also shown to playa role in prosodic phrasing.