• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.179-181
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• 2003

$F(ab`)_2$-ELISA and direct antigen coating-ELISA (DAC-ELISA) were evaluated in the detection of purified Bombyx mori nuclear polyhedrosis virus (BmNPV) and nuclear polyhedrosis virus infection in silkworm larvae inoculated with BmNPV polyhedra. Although nanogram levels of BmNPV was detected in both DAC- and $F(ab`)_2$-ELISA, similar concentrations of antigen was detected in case of F(ab’)$_2$-ELISA even at higher dilution of antibody (up to 1 : 20 K). One hundred percent nuclear polyhedrosis infection was detected 6 hrs after inoculation in BmNPV infected silkworm larvae by $F(ab`)_2$-ELISA. On the other hand, detection of 100% infection was observed only three days after inoculation in DAC-ELISA. In this study, it was observed $F(ab`)_2$-ELISA was more sensitive than DAC-ELISA in the detection of purified BmNPV as well as nuclear polyhedrosis infection in silkworm larvae.

• Asian Pacific Journal of Cancer Prevention
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• 제16권3호
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• pp.941-945
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• 2015

Semaphoring is a transmembrane receptor which participates in many cytokine-mediated signal pathways that are closely related to the angiogenesis, occurrence and development of carcinoma. The present study was designed to access the effect of mono-antibody (mAb) guided radioimmunotherapy (RIT) on skin carcinoma and investigate the potential mechanisms. Semaphoring mAb was acquired from mice (Balb/c), purified with rProtein A column; purity, concentration and activity were tested with SDS-PAGE and indirect ELISA; specificity and expression on the cutanuem carcinoma line and tissue were tested by Western blotting; morphology change was assessed by microscopy. MTT assay and colony inhibition tests were carried out to test the influence on the proliferation of tumor cells; Western blotting was also carried out for expression of apoptosis-associated (caspase-3, Bax, Bcl-2) and proliferation-related (PI3K, p-Akt, Akt, p-ERK1/2, ERK1/2) proteins and analyse the change in signal pathways (PI3K/Akt and MEK/ERK). The purity of purified semaphorin mAb was 96.5% and the titer is about $1{\times}10^6$. Western blotting showed semaphoring mAb to have specifically binding stripes with semaphoring b1b2 protein, B16F10, and A431 cells at 39KDa, 100KDa and 130KDa, respectively. Positive expression was detected both in cutanuem carcinoma line and tissue and it mostly located in cell membranes. MMT assay revealed dose-relate and time-relate inhibitory effect of semaphorin mAb on A431 and B16F10. Colony inhibition tests also showed dose-relate inhibitory effects. Western blotting demonstrated the expression of apoptosis and proliferation-related protein and changes in signal pathway. In conclusion, we demonstrated that semaphorin is highly expressed on the tumor cell-surfaces and RIT with semaphorin mAb has effect in i nhibiting proliferation and accelerating apoptosis of tumor cells.

• 한국식품위생안전성학회지
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• 제3권2호
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• pp.65-73
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• 1988

종래의 화학적, 생물학적 분석방법의 단점인 검출한도가 낮은점, 시료전처리의 복잡성, 비경제성 및 비 특이성 등을 극복하기 위해 monoclonal AB를 사용한 ELISA법으로 T-2 toxin 에 대해 특이성있는 새로운 분석법을 개발하여 다음과 같은 결과를 얻었다. 1. 종래의 GLC 및 GC-MS 분석법보다 간편하고 신뢰성이 높으며 검출한계가 0.1ppb인 고감도의 분석법을 개발하였다. 2. 본 분석법을 이용하여 Fusarium spp. 균의 T-2 생산유무를 단시간에 다량의 시료를 검색할 수 있었으며, data의 정확도가 GLC와 유사하며 GLC로는 검색할 수 없는 150ppb이하의 미량함유 시료에서도 T-2 toxin을 검색할 수 있었다. 3. 본 실험에 사용한 F. sporotichioides M-1-1 균주의 밀에 대한 최적 배양조건은 $24~27^{\circ}C$ 2주간임을 알았다.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1336-1344
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• 2017

Hepatitis B virus (HBV) is a major cause of liver cirrhosis and hepatocellular carcinoma. With recent identification of HBV receptor, inhibition of virus entry has become a promising concept in the development of new antiviral drugs. To date, 10 HBV genotypes (A-J) have been defined. We previously generated two murine anti-preS1 monoclonal antibodies (mAbs), KR359 and KR127, that recognize amino acids (aa) 19-26 and 37-45, respectively, in the receptor binding site (aa 13-58, genotype C). Each mAb exhibited virus neutralizing activity in vitro, and a humanized version of KR127 effectively neutralized HBV infection in chimpanzees. In the present study, we constructed a humanized version (HzKR359-1) of KR359 whose antigen binding activity is 4.4-fold higher than that of KR359, as assessed by competitive ELISA, and produced recombinant preS1 antigens (aa 1-60) of different genotypes to investigate the binding capacities of HzKR359-1 and a humanized version (HzKR127-3.2) of KR127 to the 10 HBV genotypes. The results indicate that HzKR359-1 can bind to five genotypes (A, B, C, H, and J), and HzKR127-3.2 can also bind to five genotypes (A, C, D, G, and I). The combination of these two antibodies can bind to eight genotypes (A-D, G-J), and to genotype C additively. Considering that genotypes A-D are common, whereas genotypes E and F are occasionally represented in small patient population, the combination of these two antibodies might block the entry of most virus genotypes and thus broadly neutralize HBV infection.

• Pediatric Infection and Vaccine
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• 제4권1호
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• pp.126-132
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• 1997

목 적 : 높은 예방접종율에도 불구하고 흥역이 계속 발생하는 것은 1차 백신 실패 또는 2 차 백신 실패 등의 논란을 자아내고 있다. 이에 저자들은 1993년 전국적으로 홍역이 유행하였을 당시 인구 밀집 대도시인 성남지역에서 그 당시 홍역에 이환되었던 일부 소아를 대상으로 특이 혈청학적 검사를 실시하였다. 대상 및 방법 : 1993년 홍역이 유행할 당시 임상진단 기준에 따라서 홍역으로 진단된 초등학교생을 대상으로 학교에 의뢰하여 93년 12월 10일부터 5일간 채혈한 후, 혈청을 분리한 후 $-20^{\circ}C$ 냉장고에 보관한 후 2개월 지나 검사하였다. Behring사의 시약을 사용하여 ELISA(Enzyme linked immunosorbent assay)방법에 의해 혈청내 홍역 특이 IgM 항체 (measles-specific IgM) 및 IgG를 검사하였다. 결 과 : 1) 총 126명 (남녀비=1:1)으로 발병시 연령은 5세 6명, 6세 11명, 7세 20명, 8세 39명, 9세 22명, 10세 11명, 11세 11명, 12세 6명으로 8세 전후에 가장 많은 빈도 보였다. 홍역은 여름에 발생하기 시작한 홍역은 9월부터 증가하기 시작하여 11월에 정점을 이루었다. 2) 홍역환자 126명중 99명(78.6%)에서 MMR접종력이 있었다. MMR접종자 99명 가운데 홍역 특이 IgM 항체에 양성은 80.8%(80/99)에서 관찰되었고, MMR 비접종자 27명 가운데 26명 (96.3%)에서 양성율을 보였다. 3) 홍역 특이 IgG 항체 반응결과 MMR 접종력이 있는 99명 중 90명(90.9%)에서, MMR 비접종군 27명중 23명(85.2%)에서 양성반응을 보였다. 흥역특이 IgG 항체반응은 MMR 접종력이 있는 아동 33명중 30례(90.1%)에서 양성을 보였다. 결 론 : MMR 접종력이 있는 아동의 상당수는 시간에 따른 항체가의 감소로 인해 홍역이 발생하였을 가능성을 배제할 수 없으나, 상당수의 환자가 IgM 항체 양성을 보인 것은 1차 백신 실패가 여전히 중요하다는 것을 의미하는 것으로 생각한다. 이를 확인하기 위해서는 보다 많은 아동을 대상으로 한 연구가 필요할 것으로 생각한다.