• Proceedings of the Korea Water Resources Association Conference
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• 2004.05b
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• pp.1247-1250
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• 2004

The amount of precipitation during El Nino over Korea increases in Summer and Winter. However, it decreases in Fall, and exhibits not much change in Spring. Especially, the amount of precipitation during September of El Nino year is much less than that of the September of non-El Nino year. The amount of precipitation during El Nino year of October and November shows similar amount of precipitation during non-El Nino year of the same period. The reason for decreasing precipitation in September is related to the weakening of the 2nd rainy season during the development of El Nino over East Asia including Korea. Insufficiency of fall precipitation during El Nino year influences drought in Spring for next year.

• Journal of Microbiology
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• v.38 no.3
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• pp.145-149
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• 2000

GroEL is a typical molecular chaperone. GroEL synthesis patterns at various culture temperatures in Escherichia coli were investigated in this study. No significant differences in the amount of GroEL produced from the chromosome were found at 30 and 37$^{\circ}C$. However, GroEL production increased 3.4-fold at 42$^{\circ}C$. GroEL synthesis was not transient but continuous at 42$^{\circ}C$, although most heat shock gene expression is known to be transient. To understand the role of the groEL structural gene, a groE promoter-lacZ fusion was constructed. Interestingly , while transcriptional fusion is not thermally inducible, it is inducible by ethanol, suggesting that the secondary structure of the groEL transcript is involved in thermal regulation of the groEL gene. Secondary structures of groE mRNA at 37 and 42$^{\circ}C$ were compared using the computer program RNAdraw. Distinct structures at the two temperatures were found, and these structures may be related to a high level of GroEL expression at 42$^{\circ}C$.

• Journal of the Korean Society of Marine Environment & Safety
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• v.26 no.1
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• pp.75-83
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• 2020

This study investigated the effects of El Niño and La Niña on coastal upwelling in the East Sea of Korea using long-term (1967-2017) water temperature observation data and Oceanic Niño Index (ONI). As a result of time series analysis of water temperature, the occurrence frequency of summer coastal upwelling was the highest in the southeastern (Ulgi ~ Gimpo) coast. In 1987-1988 and 1997-1998, when the annual fluctuations of ONI plunged more than 2.5, the water temperature in whole coast areas of the East Sea (Busan ~ Goseung) rose by 4 ~ 7 ℃. The temperature structure of the East Sea coastal water was different when El Niño was strong with ONI above 1.5 and La Niña with strong ONI below -0.8. When El Niño is strong, the water temperature anomaly in coastal waters is negative. This is due to the strong baroclinic tilting and the formation of shallow temperature stratification in the coastal waters. The strong La Niña season is opposite to the strong El Niño season, whereas the water temperature anomaly is positive. In addition, the baroclinic tilting is weaker than the time of strong El Niño and the temperature stratification is formed deeper than the time of strong El Niño. The formation of temperature stratification at shallow depths when El Niño is strong can increase the probability of occurrence coastal upwelling caused by southerly winds in the summer season. On the contrary, when La Niña is strong, occurrence of coastal upwelling is less likely even if the southerly wind blows continuously. This is because the temperature stratification is formed at deeper than when El Niño is strong.

• Korean Journal of Microbiology
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• v.32 no.1
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• pp.47-52
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• 1994

The cells of Campylobacter jejuni heat-shocked at 48${\circ}C$ for 30 min synthesized the heat shock proteins of HSP90, HSP66 and HSP60. Those heat shock proteins were found to correspond to the heat shock proteins of HSP87, HSP66 (DnaK), and HSP58 (GroEL) of E. coli, respectively. By Southern blot analysis of the chromosomal DNAs of C. jejuni with groESL and dnaK genes of E. coli as DNA probes, the heat shock genes of C. jejuni which are homologous to the E. coli groESL and dnaK genes were found to exist in the chromosomal DNA. The genomic libraries of C. jejuni were constructed with the cosmid vector pWE15 and the groEL gene of C. jejuni were cloned in E. coli B178 groEL44 temperature senstive mutant. The hybrid plasmid (pLC1) was inserted with the DNA fragment (about 5.7kb in size) containing the groEL gene. E. coli groEL44 mutant cell transformed with the pLC1 could grow at 42${\circ}C$ by synthesizing the HSP60 of C. jejuni and regained the susceptibility to the ${\lambda}$ vir phage by expression of the groEL gene in the cloned cells. These indicated that the groEL products of C. jejuni had chaperon effects by synthesizing the heat shock proteins in the cloned cells of E. coli.

• Journal of Microbiology and Biotechnology
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• v.24 no.6
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• pp.725-731
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• 2014

The classical biotype strains of the Vibrio cholerae O1 serogroup harbor the biotype-specific cholera-toxin encoding phage (CTX) $CTX^{cla}$, and the El Tor biotype strains contain CTX-1. Although the classical biotype strains have become extinct, a remnant of classical CTX phage is transferred to the El Tor biotype strains. The prototype El Tor strains, which produce the biotype-specific cholera toxin, are now being replaced by atypical El Tor variant strains producing classical biotype cholera toxin. The genome sequences of the CTX phages in atypical El Tor strains indicate that the CTX phages in atypical El Tor strains are a mosaic of $CTX^{cla}$ and CTX-1. Before the emergence of atypical El Tor stains in the early 1990s, unusual pre-seventh pandemic strains were isolated in the US Gulf Coast between 1973 and 1986. These strains have characteristics of atypical El Tor strains since they are El Tor biotype strains containing $CTX^{cla}$, yet the genome sequence of this CTX phage indicates that it is different from $CTX^{cla}$ and is therefore classified separately as $CTX^{US\;Gulf}$.

• Journal of Pharmaceutical Investigation
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• v.20 no.1
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• pp.13-18
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• 1990

Hydrocortisone (HC) sustained-release suppositories were prepared by using a solid matrix of methacrylic acid-methacrylic acid methyl ester copolymer $(Eudragit\;L_{100}^{R}:\;EL)$ as a poorly water soluble carrier and polyethylene glycole 1540 (PEG) as an water soluble carrier. HC release rate was controlled by complexation with ${\beta}-cyclodextrin$ $({\beta}-CyD)$ which was confirmed by X-ray diffractometry, IR-spectroscopy and differential scanning calorimetry. Release rate of HC from the EL-PEG matrix suppositories decreased with increase of EL contents. The release rale from $HC-{\beta}-CyD$ complex decreased in the following order: $HC-{\beta}-CyD/PEG$ > HC/PEG > $HC-{\beta}-CyD/EL_{10%}-PEG$ > $HC/EL_{10%}-PEG$ > $HC-{\beta}-CyD/EL_{15%}-PEG$ > $HC/EL_{15%}-PEG$ > $HC-{\beta}-CyD/EL_{20%}-PEG$ > $HC/EL_{20%}-PEG$. The crystallinity of HC in polymer matrix was identified using X-ray diffractometer and the surface of matrix suppositories after release test was examined by scanning electron microscopy. The sustained release of HC from these matrix suppositories was attributed to the network structure of EL.

• Journal of Environmental Health Sciences
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• v.29 no.3
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• pp.9-15
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• 2003

The modification of CYP2El activity is a matter of considerable interest because of its role in the metabolic activation of a variety of environmental toxicants. In the present study, the time-course of changes in human CYP2El activities was determined following treatment with solvents (acetone, dimethylsulphoxide or pyridine) using intact HepG2 cells transfected by human CYP2El. Hydroxylation of chlorzoxazone was used for the measurement of CYP2El activity. CYP2E1 protein level was increased upon cultivation of cells in the presence of the solvents for 24 hr. Determination of CYP2El activities after 24 ht cultivation with the solvents demonstrated that acetone or dimethylsulphoxide increased, whereas pyridine inhibited the activities. This differential effect of the solvents on CYP2El activities persisted to subsequent 24 ht. Competitive inhibition study suggested that pyridine has stronger binding affinity to CYP2E1 than acetone or dimethylsulphoxide. These results demonstrate that different binding affinity of the solvents to CYP2El plays important role in determining real CYP2El activity in intact cells after exposure to the solvents. Present study would be helpful in precise understanding of human CYP2El-mediated toxicity.

• Iberoamérica
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• v.12 no.1
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• pp.349-374
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• 2010

En realidad ningún otro cineasta español que Pedro Almodóvar ha centrado tan obsesivamente en Madrid. Casi todas sus obras se ocupan de la capital de España como espacio cinematográfico. Como uno de los protagonistas de La Movida, Almodóvar es un producto de Madrid de la Transición que dió luz a la nueva sociedad democrática. En principio, Madrid en las películas de Almodóvar se convierte en un espacio carnavalesco desde la perspectiva bajtiniana. El crítico ruso ha exaltado la plaza pública de los pueblos medievales europeos como el espacio carnavalesco en el que los contactos libres y familiares se realizan y el principio de lo corporal y lo material predomina. Para Bajtín, el carnaval es básicamente un rito político en el que el individuo parece dotado de una segunda vida que le permite establecer nuevas relaciones verdaderamente humanas con sus semejantes sin ninguna represión. Así que en el mundo carnavalesco todo lo sagrado, lo oficial y lo privilegiado se destronan, y en cambio lo grosero, lo extra-oficial y lo bajo se reivindican. Como los participantes del carnaval, los personajes de Almodóvar disfrutan de una segunda vida en un Madrid del mundo al revés. Por otra parte, la representación carnavalesca de Madrid tiene una tradición rica y larga en el arte español. Entre muchos artistas Goya, Arniches, Valle-Inclán y Buñuel son los antecedentes destacados de esta tradición. Así pues se puede concluir que la estética del cine de Almodóvar está anclada en la tradición hispánica del carnavalismo más bien que la estética global de la posmodernidad.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.27 no.1
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• pp.111-118
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• 2001

A novel melanogenic inhibitory compound, Phytoclear-EL1 (5,10-diacetyl-3-benzoyllathyrol) was isolated from Euphorbiae lathyridis. The effects of EL1 on culture normal human melanocytes (NHM) were assessed. EL1 reduced the melanin synthesis of NHM by 40% at a concentration of 2 $\square$g/ml without any apparent cytotoxicity. We also have found that the treatment of the cells with EL1 decreased the tyrosinase activity by 29% in situ. To elucidate the action mechanism of EL1, we investigated the changes in mRNA levels of tyrosinase, TRP-1 and TRP-2 using RT-PCR technique. AS a result, the mRNA levels of tyrosinase, TRP-1 and TRP-2 were markedly reduced by EL1 treatment. These results suggest that Phytoclear-EL1 is a novel whitening agent that is effective in human melanocytes.

• Proceedings of the KIEE Conference
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• 2015.07a
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• pp.1132-1133
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• 2015

In this paper, we are presents production and operation characteristics for inorganic EL phosphors device using screen printed. The EL device is composed as ITO PET / EL phosphors dielectric $(2{\times}2cm^2)/TiO^2$ paste/Ag electrode. At 100Vac 400Hz, the luminescence of inorganic EL phosphors were $60.33cd/m^2$ of red phosphor, $42.12cd/m^2$ of green phosphor and $58.45cd/m^2$ of blue phosphor. The output current was 12.57 mA, 17.11 mA and 11.98 mA, respectively. The inorganic EL phosphors of EL device are increasing efficiency EL device.