• BMB Reports
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• v.41 no.8
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• pp.593-596
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• 2008

ADPKD (Autosomal Dominant Polycystic Kidney Disease) is characterized by the progressive expansion of multiple cystic lesions in the kidneys. ADPKD is caused by mutations in Ed-pl. consider PKD1 and PKD2. Recently a relation between c-myc and the pathogenesis of ADPKD was reported. In addition, c-Myc is a downstream effector of PKD1. To identify the gene regulated by PKD2 and c-Myc, we performed gene expression profiling in PKD2 and c-Myc overexpressing cells using a human 8K cDNA microarray. NCAM (neuronal cell adhesion molecule) levels were significantly reduced in PKD2 overexpressing systems in vitro and in vivo. These results suggest that NCAM is an important molecule in the cystogenesis induced by PKD2 overexpession.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.2
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• pp.211-216
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• 2003

The objective of this study was to determine the degradability of cassava chip (CC), cassava waste (CW), yellow sweet potato (YP), white sweet potato (WP), purple sweet potato (PP), corn meal (CM), and rice bran (RB) using in situ technique. Two ruminally fistulated steers with an average weight of $303{\pm}10kg$ were used to determine in situ degradabilities of DM and OM. Seven feed sources were weighted in nylon bags ($38{\mu}m$ pore size) and incubated ruminally for 1, 2, 4, 6, 8, 12, 24, and 48 h. The results showed that asymptote (a+b) and effective degradability (ED) of DM of energy sources ranked from the highest to the lowest; CC, YP, WP, PP, RB, CW, and CM (99.3, 92.5; 97.6, 87.9; 97.5, 87.9; 97.2, 87.8; 87.5, 63.6; 78.6, 63.0 and 81.7; 59.3, respectively) and for OM asymptote (a+b) and effective degradability (ED) were similar to those of degradation of DM (99.4, 93.4; 98.8, 89.8; 98.5, 89.4; 98.4, 88.1; 92.4, 65.8; 85.1, 66.9 and 83.6, 63.3, respectively). It was concluded that disappearance characteristic of CC was the highest and it may potentially facilitate the achievement of optimal ruminal availability of energy: protein especially with NPN for microbial protein synthesis.

• Journal of the Korean Chemical Society
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• v.46 no.3
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• pp.205-212
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• 2002

Bioactive compounds of Chung-pi methoxy flavonoids were isolated by column chromatography and preparative chromatography, and investigated on the smoothing effect for the tracheal smooth muscle of rats. The tracheal smooth muscles of rats were treated with acetylcholine ($ED_{50}:3${\times}$10^{-6}M$) and with chromatographic fractions. We found that six-methoxylated flavonoid (nobiletin) was the most active compound fro the smoothing effect of which the contracted tracheal smooth muscle was screened with further separated ethyl acetate fraction. This result shows how nobiletin and its analogues could be using as a promising drug of bronchial asthma.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.4
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• pp.344-350
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• 2006

Thermoplasma acidophilum is a thermoacidophilic archaeon that grows optimally at $59^{\circ}C$ and pH 2. Along with another thermoacidophilic archaeon, Sulfolobus solfataricus, it is known to metabolize glucose by the non-phosphorylated Entner-Doudoroff (nED) pathway. In the course of these studies, the specific activities of glyceraldehyde dehydrogenase and glycerate kinase, two enzymes that are involved in the downstream part of the nED pathway, were found to be much higher in T. acidophilum than in S. solfataricus. To characterize glycerate kinase, the enzyme was purified to homogeneity from T. acidophilum cell extracts. The N-terminal sequence of the purified enzyme was in exact agreement with that of Ta0453m in the genome database, with the removal of the initiator methionine. Furthermore, the enzyme was a monomer with a molecular weight of 49kDa and followed Michaelis-Menten kinetics with $K_m$ values of 0.56 and 0.32mM for DL-glycerate and ATP, respectively. The enzyme also exhibited excellent thermal stability at $70^{\circ}C$. Of the seven sugars and four phosphate donors tested, only DL-glycerate and ATP were utilized by glycerate kinase as substrates. In addition, a coupled enzyme assay indicated that 2-phosphoglycerate was produced as a product. When divalent metal ions, such as $Mn^{2+},\;CO^{2+},\;Ni^{2+},\;Zn^{2+},\;Ca^{2+},\;and\;Sr^{2+}$, were substituted for $Mg^{2+}$ the enzyme activities were less than 10% of that obtained in the presence of $Mg^{2+}$. The amino acid sequence of T. acidophilum glycerate kinase showed no similarity with E. coli glycerate kinases, which belong to the first glycerate kinase family. This is the first report on the biochemical characterization of an enzyme which belongs to a member of the second glycerate kinase family.

• Journal of East-West Nursing Research
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• v.24 no.1
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• pp.10-19
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• 2018

Purpose: The purpose of this study was to examine factors affecting the performance of the forensic nursing role among emergency department (ED) nurses. Methods: Data collection was conducted on 125 nurses of EDs in 9 medical centers in U metropolitan city from June 20 to July 20, 2016. Data were analyzed by descriptive statistics, t-test, ANOVA, Pearson's correlation coefficients, and stepwise multiple regression using SPSS Win 21.0 program. Results: There were statistically significant differences in the performance of the forensic nursing role according to the types of emergency medical institutions, perception of the forensic nursing and forensic nurse. Performance of the forensic nursing role had positive relationships with the awareness of the forensic nursing role and forensic nursing competency. Factors affecting the performance of forensic nursing role were forensic nursing role awareness, the types of emergency medical institutions and forensic nursing competency. These factors explained 40.8% of the total variance. Conclusion: The results suggest that awareness of the forensic nursing role, forensic nursing competency and the types of emergency medical institutions have influences on the performance of forensic nursing role. Therefore, it is necessary to design interventions suitable for the types of emergency medical institutions for ED nurses to enhance the awareness of forensic nursing role and to reinforce forensic nursing competency.

• Archives of Pharmacal Research
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• v.21 no.2
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• pp.198-206
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• 1998

Fourty eight derivatives of 2-(1-oxyalkyl)-1,4-dioxy-9,10-anthraquinone were synthesized, and their antitumor activity was evaluated. On the whole, 2-(1-hydroxyalkyl)-1,4-dihydroxy-9,10-anthraquinones (DHAQ=1,4-dihydroxy-9,10-anthraquinone) showed stronger cytotoxic activity against L1210 cells than 2-(l-hydroxyalkyl)-1,4-dimethoxy-9,10-anthraquinones(DMAQ =1,4-dimethoxy-9,10-anthraquinone), implying that free hydroxy groups at C-1 and C-4 of the anthraquinone structure are necessary for the cytotoxic activity. The bioactivity of 2-(lhydroxyalkyl)-DHAQ derivatives differed according to the size of alkyl group at C-1;while the elongation of alkyl group over 7 carbon atoms failed to enhance the bioactivity, the derivatives possessing alkyl moiety of 1-6 carbon atoms showed an increase in the cytotoxicity and the antitumor activity in Sarcoma-180; 2-hydroxymethyl-DHAQ ($ED_{50}$, $15\mu\textrm{g}$/ml; T/C, 125%), 2-(1 -hydroxyethyl)-DHAQ($1.9{\mu}g/ml;139.2%)$;, 2-(1-hydroxypropyl)-DHAQ ($7.2{\mu}g$/ml; 135.1%), 2-(1-hydroxybutyl)-DHAQ ($10.2{\mu}g/ml; 125.3%)$, 2-(1-hydroxypentyl)-DHAQ ($23.7{\mu}g/ml; 110.1%$). and 2-(1-hydroxyhexyl)-DHAQ ($58{\mu}g/ml;108%$). Next, 2-(1-Hydroxyalkyl)-DHAQ derivatives were acetylated to produce 2-(1-acetoxyalkyl)-DHAQ analogues. Although the acetylation somewhat enhanced the cytotoxicity, but not the antitumor action. In addition, the presence of phenyl group at $C-1^{l}$ enhanced the cytotoxicity and the T/C value, compared to alkyl groups of same size; 2-(1-hydroxy-1-phenyl)-DHAQ ($ED_{50}$, $5.6{\mu}g$, T/C, 137%).

• Structural Engineering and Mechanics
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• v.76 no.1
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• pp.83-99
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• 2020

Shear walls are structural members in buildings that are used extensively in reinforced concrete frame buildings, and almost exclusively in the UK, regardless of whether or not they are actually required. In recent years, the UK construction industry, led by the Concrete Centre, has questioned the need for such structural elements in low to mid-rise reinforced concrete frame buildings. In this context, a typical modern, 5-storey residential building is studied, and its existing shear walls are replaced with columns as used elsewhere in the building. The aim is to investigate the impact of several design variables, including concrete grade, column size, column shape and slab thickness, on the building's structural performance, considering two punching shear limits (V_Ed/V_Rd,c), lateral drift and accelerations, to evaluate its maximum possible height under wind actions without the inclusion of shear walls. To facilitate this study, a numerical model has been developed using the ETABS software. The results demonstrate that the building examined does not require shear walls in the design and has no lateral displacement or acceleration issues. In fact, with further analysis, it is shown that a similar building could be constructed up to 13 and 16 storeys high for 2 and 2.5 punching shear ratios (V_Ed/V_Rd,c), respectively, with adequate serviceability and strength, without the need for shear walls, albeit with thicker columns.

• CELLMED
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• v.6 no.1
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• pp.6.1-6.6
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• 2016

Polycystic Ovarian Syndrome (PCOS) has now become more common in occurrence in women of reproductive age, particularly in urban and semi-urban population in India. So there is a need to investigate this phenomenon taking into consideration various aspects including possible treatment method to ameliorate/eradicate this syndrome, which has far reaching socio-economic impact and consequences, in view of infertility and irregular menstrual cycles frequently associated with this syndrome. Homeopathy is a branch of traditional alternative medicine which is gaining popularity in India and some other developing countries, as also in some of the developed countries in Europe. With this background scenario, we have made an attempt to treat cases of confirmed PCOS and tried to compare the relative efficacy of two potentized homeopathic drugs, namely, Lycopodium clavatum (Lyco) and Calcarea carbonica (Calc), most frequently used by homeopathic practitioners, selecting different potencies of the drugs, depending on condition/guiding symptom(s) of the patients. While the main focus was pointed on total/partial removal of cysts, data pertaining to different PCOS associated symptoms were also compared for the sake of learning if the two drugs had differential effects on these symptoms also. The study parameters in this investigation included: regularity/irregularity of menstrual cycle, presence/absence of acne, hirsutism, male type alopecia, acanthosis nigricans, body/mass index (BMI) and waist-hip ratio. Overall results provided clear evidences that both these homeopathic drugs had great ameliorating effects on PCOS, although each drug had a little different effect in respect of the individual parameters of this study.

• Journal of Medicine and Life Science
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• v.17 no.1
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• pp.7-15
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• 2020

The field triage guidelines have been widely implemented in the Korean trauma system. This study aimed to evaluate and validate whether it is reliable to use the field triage guidelines for predicting severe traumatic brain injury (TBI) and traumatic spinal injury (TSI) patients. This study retrospectively analyzed in-hospital cohort registries of all TBI and TSI patients, who visited the emergency department (ED) of the Jeju National University Hospital from 1 January 2013 to 31 December 2015. The primary outcome was defined as TBI and TSI patients with an injury severity score (ISS)>15. Secondary outcomes were defined as cases in which one or more of the following conditions: in-hospital death, ISS>15, admission to the intensive care unit, emergency surgery. We enrolled 14,889 TBI and TSI patients who visited ED, of which 7,966 (53.5%) were triage positive. The overall sensitivity, specificity and area under the curve (AUC) of the full cumulative field triage guidelines step's model (Step 1+3+4 criteria) for primary outcome were 82.8%, 47.0%, and 0.646, respectively. In the results for secondary outcomes, the specificity did not show a significant difference, but the sensitivity decreased to 66.5% and AUC to 0.568. The results of this study suggest that further optimization of the field triage guidelines is needed to identify high-risk TBI and TSI patients.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.23 no.2
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• pp.81-85
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• 2013

We performed analysis of composition and structure of coating layers by using ED-XRF and TOF-SIMS for some passion topaz of Swarovski which developed recently as new surface treatment of TCF (thermal color fusion) technique. In addition, we compared differences between Ti-coated topaz (Mystic topaz) and new treated colored topazes (passion topaz) with magnification observation and simplified durability test. As a result, we can observe similar characteristic clues in Ti-coated topaz and passion topaz by magnified observation. According to results of depth profile by TOF-SIMS, we can know that topaz is treated by multi-layer coating or surface diffusion coating. Moreover, the passion topaz which is treated by chemical reaction between metal elements shows more stable chemical resistance and higher Mohs' hardness than Ti-coated topaz.