• Computers and Concrete
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• v.11 no.2
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• pp.123-148
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• 2013

The nonlinear finite element method with eight noded isoparametric quadrilateral element for concrete and two noded element for reinforcement is used for the prediction of the behavior of reinforcement concrete structures. The disturbed state concept (DSC) including the hierarchical single surface (HISS) plasticity model with associated flow rule with modifications is used to characterize the constitutive behavior of concrete both in compression and in tension which is named DSC/HISS-CT. The HISS model is applied to shows the plastic behavior of concrete, and DSC for microcracking, fracture and softening simulations of concrete. It should be noted that the DSC expresses the behavior of a material element as a mixture of two interacting components and can include both softening and stiffening, while the classical damage approach assumes that cracks (damage) induced in a material treated acts as a void, with no strength. The DSC/HISS-CT is a unified model with different mechanism, which expresses the observed behavior in terms of interacting behavior of components; thus the mechanism in the DSC is much different than that of the damage model, which is based on physical cracks which has no strength and interaction with the undamaged part. This is the first time the DSC/HISS-CT model, with the capacity to account for both compression and tension yields, is applied for concrete materials. The DSC model allows also for the characterization of non-associative behavior through the use of disturbance. Elastic perfectly plastic behavior is assumed for modeling of steel reinforcement. The DSC model is validated at two levels: (1) specimen and (2) practical boundary value problem. For the specimen level, the predictions are obtained by the integration of the incremental constitutive relations. The FE procedure with DSC/HISS-CT model is used to obtain predictions for practical boundary value problems. Based on the comparisons between DSC/HISS-CT predictions, test data and ANSYS software predictions, it is found that the model provides highly satisfactory predictions. The model allows computation of microcracking during deformation leading to the fracture and failure; in the model, the critical disturbance, Dc, identifies fracture and failure.

• Korean Journal of Environmental Agriculture
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• v.39 no.3
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• pp.246-252
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• 2020

BACKGROUND: Pesticides are broadly used to control weeds and pests, and the residues remaining in crops are managed in accordance with the MRLs (maximum residue limits). Therefore, an analytical method is required to quantify the residues, and we conducted a series of analyses to select and validate the quick and simple analytical method for tolpyralate in five agricultural products using QuEChERS (quick, easy, cheap, effective, rugged and safe) method and LC-MS/MS (liquid chromatography-tandem mass spectrometry). METHODS AND RESULTS: The agricultural samples were extracted with acetonitrile followed by addition of anhydrous magnesium sulfate, sodium chloride, disodium hydrogencitrate sesquihydrate and trisodium citrate dihydrate. After shaking and centrifugation, purification was performed with d-SPE (dispersive-solid phase extraction) sorbents. To validate the optimized method, its selectivity, linearity, LOD (limit of detection), LOQ (limit of quantitation), accuracy, repeatability, and reproducibility from the inter-laboratory analyses were considered. LOQ of the analytical method was 0.01 mg/kg at five agricultural products and the linearity of matrix-matched calibration were good at seven concentration levels, from 0.0025 to 0.25 mg/L (R²≥0.9980). Mean recoveries at three spiking levels (n=5) were in the range of 85.2~112.4% with associated relative standard deviation values less than 6.2%, and the coefficient of variation between the two laboratories was also below 13%. All optimized results were validated according to the criteria ranges requested in the Codex Alimentarius Commission (CAC) and Ministry of Food and Drug Safety (MFDS) guidelines. CONCLUSION: In conclusion, we suggest that the selected and validated method could serve as a basic data for detecting tolpyralate residue in imported and domestic agricultural products.

• Korean Journal of Environmental Agriculture
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• v.39 no.2
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• pp.130-137
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• 2020

BACKGROUND: Mefentrifluconazole and triticonazole are the triazole fungicides. The maximum residue levels for agricultural products need to be set up. Therefore, development of the official analytical method for determination of mefentrifluconazole and triticonazole residues from agricultural crops was necessary due to safety management, and then a simultaneous analytical method was developed for the determination of mefentrifluconazole and triticonazole in agricultural crops. METHODS AND RESULTS: Samples were extracted using acetonitrile and purified using dispersive solid phase extraction, and then detected with liquid chromatograph-tandem mass spectrometry (LC-MS/MS). Matrix-matched calibration curves (0.0025-0.25 ㎍/mL) were linear into a sample extract with r²>0.99. For validation, the recovery test was carried out at three fortification levels (LOQ, 10 LOQ and 50 LOQ) from agricultural samples. The results for mefentrifluconazole and triticonazole ranged between 92.3 to 115.3% and 91.4 to 108.5%, respectively and RSD (relative standard deviation) values were also below 6.0%. Furthermore, inter-laboratory was conducted to validate the method. CONCLUSION: All values were corresponded with the criteria ranges requested by both the CODEX (CAC/GL 40-1993, 2003) and MFDS guidelines (2016). Therefore, the proposed method can be used as an official analytical method for determination of mefentrifluconazole and triticonazole (triazole fungicides) in the Republic of Korea.

• Journal of Information Management
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• v.8 no.5
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• pp.130-140
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• 1975

이용자(利用者) 중심(中心)의 대화방식(對話方式) 시스팀을 개발(開發)하고 시험(試驗)하였다. 2년(年)동안 280개의 프로파일로 CA - Condensates 데이터 베이스내에서 정보(情報)를 검색(檢索)해 보았다. 그 시스팀의 작업능률(作業能率)을 사용(使用)한 데이터 베이스와 하드웨어의 구조(構造), 소프트웨어 패키지, 이용자(利用者)의 수(數), 그리고 정보(情報)센터에서 제공(提供)한 보조(補助)에 따라 평가(評價)하였다. 대화식(對話式) 검색방법(檢索方法)이나 이용자(利用者)의 요구(要求)를 양화(量化)하는 것, 그리고 여러가지 분야별(分野別)로 검색(檢索)하는 방법(方法) 등 이용자(利用者)의 요구(要求)를 보다 충실(充實)하게 만족(滿足)시킬 수 있는 여러가지 방법(方法)들을 논의(論議)하였다. 모든 정보(情報)시스팀의 목적(目的)은 적시(適時)에 올바른 정(情)보를 적자(適者)에게 제공(提供)하는 것이라고 잘 알려져 있다. 다시 말해서, 정보(情報)시스팀의 주요목적(主要目的)은 이용자(利用者)의 요구(要求)를 충족(充足)시켜 주는 것이다. 이러한 관념(觀念)의 변화(變化)는 정보처리(情報處理)에 큰 변화(變化)를 가져와 정보(情報)시스팀의 적응성(適應性)을 증가(增加)시키기 위해 여러 가지 새로운 방법(方法)들이 개발(開發)되었다. 정보(情報)시스팀들 사이의 상호작용(相互作用), 사람과 컴퓨터와의 대화(對話), 그리고 컴퓨터의 조력(助力)으로 이용자(利用者)의 프로파일을 작성(作成)하는 것 등은 이용자(利用者)의 요구(要求)를 존중(尊重)하고, 필요(必要)한 정보(情報)를 쉽게 찾기 위해서 고안(考案)된 것이다. 한편, 우리들은 이용자(利用者)의 특성(特性)이나 배경(背景), 그리고 문헌(文獻)에 대한 그의 습성(習性) 등에 대해서 얼마나 모르고 있는가를 깨닫게 된다. 상기(上記)의 내용(內容)을 요약(要略)하면, 근대정보(近代情報) 시스팀은 다음의 필요조건(必要條件)을 만족(滿足)해야 한다. 1) 이용자(利用者)의 요구(要求)에 호응(呼應)하기 위해서 적합(適合)한 일련(一連)의 정보(情報)를 제공(提供)해야 한다. 2) 이용자(利用者)와의 관계(關系)에서 시스팀은 적극적(積極的)이어야 한다. 즉, 프로파일 검색공식(檢索公式)에서 유발(誘發)되는 잘못을 지시(指示)하고 지적(指適)할 수 있어 야 한다. 나아가서 컴퓨터의 조력(助力)으로 프로파일을 작성(作成)하고 쉽게 수정(修正)할 수 있는 바람직하다. 우리들은 상기(上記)한 필수조건(必須條件)에 일치(一致)하도록 노력(努力) 하고 Bratislava에 위치(位置)하고 있는 슬로박 과학원(科學院)의 무기화학연구소(無機化學硏究所)와 화학공업경제연구소(化學工業經濟硏究所)가 공동(共同)으로 개발(開發)한 시스팀이 상기(上記)의 특수성(特殊性) 최소(最少)한 몇가지 도입(導入)하였다. 시스팀은 CACS라는 작업명하(作業名下)에서 운영(運營)되었다. 대략 15개월동안 280여개의 프로파일로 CA- Condensates 데이터베이스에서 정보(情報)를 검색(檢索)하여 이용자(利用者)들에게 최신정보(最新情報) 주지사업(周知事業)을 해 보았다. 우리들은 시스팀의 작업능률(作業能率)을 평가(評價)하고 이용자(利用者)의 요구(要求)를 만족(滿足)시키는 최선(最善)의 방법(方法)을 모색(摸索)하였다. 우선 이용자(利用者)와 시스팀사이를 밀접(密接)하게 연결(連結)하는데 중점(重點)을 두었다. 작업능률(作業能率Z)을 분석(分折)해 보고 우리들은 효율(效率)에 영향(影響)을 주는 인자(因子)는 다음의 4가지 주요분류(主要分類)에 속한다는 결론(結論)을 얻었다. (1) 검색(檢索)하는 데이터 베이스 (2) 情報시스팀, 소프트웨어 패키지 그리고 하드웨어 구조(構造) (3) 이용자(利用者)의 특성(特性)과 그의 요구(要求) (4) 정보(情報)센터가 이용자(利用者)에게 제공(提供)하는 보조(補助)

• Nutrition Research and Practice
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• v.2 no.4
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• pp.289-294
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• 2008

Pre-B-cell leukemia transcription factor 1 (PBX1), which is located on chromosome 1q23, was recently reported to be associated with type 2 diabetes mellitus. We examined whether single nucleotide polymorphisms (SNPs) of the PBX1 gene are associated with overweight/obesity in a Korean population. We genotyped 66 SNPs in the PBX1 gene and investigated their association with clinical phenotypes found in 214 overweight/obese subjects and 160 control subjects using the Affymetrix Targeted Genotyping chip array. Seven SNPs (g.+75l86C>T, g.+78350C>A, g.+80646C>T, g.+138004C>T, g.+185219G>A, g.+191272A>C, and g.+265317T>A) were associated with the risk of obesity in three models (codominant, dominant, and recessive) (P=0.007-0.05). Haplotype 1 (CAC) and 3 (TAC) of block 3 and haplotype 2 (GGAAT) of block 10 were also strongly associated with the risk of obesity. In the control group, subjects that had homozygote for the major allele for both g.+185219G>A and g.+191272A>C showed lower high density lipoprotein-cholesterol (HDL-C) level compared to those possessing the minor allele, suggesting that the association between the homozygote for the major allele for both g.+185219G>A and g.+191272A>C and HDL-C is attributable to the increased risk of obesity. This study suggests that the PBX1 gene is a possible risk factor in overweight/obese patients.

• Computers and Concrete
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• v.33 no.5
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• pp.497-507
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• 2024

In squat reinforced concrete walls, the displacement capacity for lateral deformation is low and the ability to resist the axial load can quickly be lost, generating collapse. This work consists of testing two squat reinforced concrete walls. One of the specimens is built with conventional detailing of reinforced concrete walls, while the second specimen is built applying an alternative design, including stirrups along the diagonal of the wall to improve its ductility. This solution differs from the detailing of beams or coupling elements that suggest building elements equivalent to columns located diagonally in the element. The dimensions of both specimens correspond to a wall with a low aspect ratio (1:1), where the height and length of the specimen are 1.4 m, with a thickness of 120 mm. The alternative wall included stirrups placed diagonally covering approximately 25% of the diagonal strut of the wall with alternative detailing. The walls were tested under a constant axial load of 0.1f'cAg and a cyclic lateral displacement was applied in the upper part of the wall. The results indicate that the lateral strength is almost identical between both specimens. On the other hand, the lateral displacement capacity increased by 25% with the alternative detailing, but it was also able to maintain the 3 complete hysteretic cycles up to a drift of 2.5%, reaching longitudinal reinforcement fracture, while the base specimen only reached the first cycle of 2% with rapid degradation due to failure of the diagonal compression strut. The alternative design also allows 46% more energy dissipation than the conventional design. A model was used to capture the global response, correctly representing the observed behavior. A parametric study with the model, varying the reinforcement amount and aspect ratio, was performed, indicating that the effectiveness of the alternative detailing can double de drift capacity for the case with a low aspect ratio (1.1) and a large longitudinal steel amount (1% in the web, 5% in the boundary), which decreases with lower amounts of longitudinal reinforcement and with the increment of aspect ratio, indicating that the alternative detailing approach is reasonable for walls with an aspect ratio up to 2, especially if the amount of longitudinal reinforcement is high.

• Journal of Food Hygiene and Safety
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• v.34 no.1
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• pp.22-29
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• 2019

Validamycin A is an aminoglycoside fungicide produced by Streptomyces hygroscopicus that inhibits trehalase. The purpose of this study was to develop a method for detecting validamycin A in agricultural samples to establish MRL values for use in Korea. The validamycin A residues in samples were extracted using methanol/water (50/50, v/v) and purified with a hydrophilic-lipophilic balance (HLB) cartridges. The analyte was quantified and confirmed by liquid chromatograph-tandem mass spectrometer (LC-MS/MS) in positive ion mode using multiple reaction monitoring (MRM). Matrix-matched calibration curves were linear over the calibration ranges (0.005~0.5 ng) into a blank extract with $R^2$ > 0.99. The limits of detection and quantification were 0.005 and 0.01 mg/kg, respectively. For validation validamycin A, recovery studies were carried out three different concentration levels (LOQ, $LOQ{\times}10$, $LOQ{\times}50$, n = 5) with five replicates at each level. The average recovery range was from 72.5~118.3%, with relative standard deviation (RSD) less than 10.3%. All values were consistent with the criteria ranges requested in the Codex guidelines (CAC/GL 40-1993, 2003) and the NIFDS (National Institute of Food and Drug Safety) guideline (2016). Therefore, the proposed analytical method is accurate, effective and sensitive for validamycin A determination in agricultural commodities.

• Journal of Food Hygiene and Safety
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• v.31 no.5
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• pp.319-326
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• 2016

Colistin is a last resort antimicrobial agent against multi-drug resistant Gram-negative bacteria. This study was conducted to develop an analytical method to determine colistin in fish and shrimp. The analytes were confirmed and quantified via liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the positive ion mode using multiple reaction monitoring (MRM). The sample was extracted with acidified 5% methanol (containing 0.5% formic acid). Then, solid phase extraction (SPE) was used for cleanup. Matrix-matched calibration curves were linear over the calibration ranges (0.05-1.2 mg/kg) for all the analytes into blank sample with $r^2$ > 0.99. All the values fulfilled the criteria requested by the Codex guidelines. Average recoveries ranged from 85.9% to 107.9%. The repeatability of measurements, expressed as the coefficient of variation (CV, %), was less than 15%. The limit of detection (LOD) was 0.02 mg/kg, and the limit of quantitation (LOQ) was 0.05 mg/kg. This improved method showed higher accuracy and acceptable sensitivity to meet the CAC guideline requirements and is applicable for the analysis of residual colistin (A+B) in fish and shrimp.

• Computers and Concrete
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• v.14 no.5
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• pp.599-612
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• 2014

This paper presents the effects of the construction stages using time dependent material properties on the structural behaviour of concrete arch dams. For this purpose, a double curvature Type-5 arch dam suggested in "Arch Dams" symposium in England in 1968 is selected as a numerical example. Finite element models of Type-5 arch dam are modelled using SAP2000 program. Geometric nonlinearity is taken into consideration in the construction stage analysis using P-Delta plus large displacement criterion. In addition, the time dependent material strength variations and geometric variations are included in the analysis. Elasticity modulus, creep and shrinkage are computed for different stages of the construction process. In the construction stage analyses, a total of 64 construction stages are included. Each stage has generally $6000m^3$ concrete volume. Total duration is taken into account as 1280 days. Maximum total step and maximum iteration for each step are selected as 200 and 50, respectively. The structural behaviour of the arch dam at different construction stages has been examined. Two different finite element analyses cases are performed. In the first case, construction stages using time dependent material properties are considered. In the second case, only linear static analysis (not considered construction stages) is taken into account. Variation of the displacements and stresses are obtained from the both analyses. It is highlighted that construction stage analysis using time dependent material strength variations and geometric variations has an important effect on the structural behaviour of arch dams. The maximum longitudinal, transverse and vertical displacements obtained from construction stages and static analyses are 1.35 mm and 0 mm; -8.44 and 6.68 mm; -4.00 and -9.90 mm, respectively. In addition, vertical displacements increase from the base to crest of the dam for both analyses. The maximum S11, S22 and S33 stresses are obtained as 1.60MPa and 2.84MPa; 1.39MPa and 2.43MPa; 0.60MPa and 0.50MPa, respectively. The differences between maximum longitudinal, transverse, and vertical stresses obtained from construction stage and static analyses are 78%, 75%, and %17, respectively. On the other hand, there is averagely 12% difference between minimum stresses for all three directions.

• Korean Journal of Environmental Agriculture
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• v.41 no.2
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• pp.135-151
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• 2022

BACKGROUND: The β-agonists known as phenyl ethanolamine derivatives have a conjugated aromatic ring with amino group. They are used as tocolytic agents and bronchodilator to human and animal generally, and some of them are used as growth promoters to livestock. METHODS AND RESULTS: β-agonists in samples were extracted by 0.4 N perchloric acid and ethyl acetate. The target compounds were analyzed by liquid chromatography-electrospray tandem mass spectrometry (LC-ESI-MS/MS). Validation of method was performed according to CODEX guidelines (CAC/GL-71). The matrix matched calibration gave correlation coefficients>0.98, and the obtained recoveries were in the range of 62.0-109.8%, with relative standard deviation ≤ 20.1%. In addition, a survey was performed to inspect any residual β-agonist from 100 samples of livestock and fishery products and ractopamine was detected in one of the 100 samples. CONCLUSION(S): In this study, we established the analytical method for β-agonists through using the expanded target compounds and samples. And we anticipate that the established method would be used for analysis to determine veterinary drug residues in livestock and fishery products.