• KSBB Journal
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• v.9 no.3
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• pp.294-298
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• 1994

The medium additives such as CaCl2, glucose, fructose, glutamine, glutamate and lipids were examined to enhance recombinant ${\beta}$-galactosldase(${\beta}$-gal) production in batch and continuous two-stage bioreactor systems. The presence of each medium additive such as CaCl2, fructose, glutamate, cholesterol and tocopherol at AcNPV infection of Sf 21 cells had an effect on improved ${\beta}$-gal production. The recombinant ${\beta}$-gal production using the infection media supplemented with a mixture of 30mM $CaCl_2$, 2.2mM fructose, 4.1mM glutamate and 0.34mM cholesterol was increased by about 40%.

• Journal of People, Plants, and Environment
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• v.23 no.4
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• pp.445-453
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• 2020

Background and objective: Calcium chloride (CaCl₂) and sodium chloride (NaCl) are commonly used as a deicing agent in South Korea and penetrate the soil on the roadside, causing damage to plants. This study was conducted to investigate the salinity reduction effect of Pennisetum alopecuroides and the chemical characteristics of soil leachate. Methods: The plants were treated with five different concentrations of CaCl₂ (0, 1, 2, 5, and 10g·L^-1) and were grouped into the Cont., C1, C2, C5, and C10 groups. CaCl₂ of 200 m·L^-1 was sprayed to each plant once every two weeks. The growth of P. alopecuroides (plant height, leaf length, leaf width and the number of leaves) was measured. The level of EC and pH, and exchangeable cations (K⁺, Ca²⁺, Na⁺, and Mg²⁺) in the leachate of soil was monitored. Results: The pH of soil leachate decreased as the CaCl₂ concentration increased, and the EC increased significantly. The content of K⁺ did not change significantly until the concentration of CaCl₂ reached 5 g·L^-1, but the content of Ca²⁺, Na⁺, and Mg²⁺ significantly increased. The plant height, leaf length, and leaf width of P. alopecuroides showed the highest value in CaCl₂ 1 g·L^-1 followed by CaCl₂ 2 g·L^-1 and the control group. Root fresh weight was the highest in CaCl₂ 2 g·L^-1. On the other hand, there was no change in the shoot fresh weight, dry weight and root dry weight, and P. alopecuroides growth inhibition at the concentration of 5 g·L^-1 or higher in the plant height and leaf length. Conclusion: P. alopecuroides is relatively highly salt-tolerant and can improve the salt damaged soil by lowering the content of the salt-based exchangeable K⁺ ions.

• Geomechanics and Engineering
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• v.20 no.2
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• pp.165-174
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• 2020

The enzyme-induced carbonate precipitation (EICP) method has been investigated to improve the hydro-mechanical properties of natural soil deposits. This study was conducted to explore the stiffness evolution during various stress scenarios. First, the optimal concentration of urea, CaCl₂, and urease for the maximum efficiency of calcite precipitation was identified. The results show that the optimal recipe is 0.5 g/L and 0.9 g/L of urease for 0.5 M CaCl₂ and 1 M CaCl₂ solutions with a urea-CaCl₂ molar ratio of 1.5. The shear stiffness of EICP-treated sands remains constant up to debonding stresses, and further loading induces the reduction of S-wave velocity. It was also found that the debonding stress at which stiffness loss occurs depends on the void ratio, not on cementation solution. Repeated loading-unloading deteriorates the bonding quality, thereby reducing the debonding stress. Scanning electron microscopy and X-ray images reveal that higher concentrations of CaCl₂ solution facilitate heterogeneous nucleation to form larger CaCO₃ nodules and 11-12 % of CaCO₃ forms at the interparticle contact as the main contributor to the evolution of shear stiffness.

• The Korean Journal of Physiology
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• v.28 no.2
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• pp.143-150
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• 1994

The whole-cell mode of the patch clamp technique was used to study $Ca^{2+}-activated\;Cl^-\;current$ $(I_{Cl_{Ca}})$ in gastric antral myocytes. Extracellular application of caffeine evoked $Ca^{2+}-activated\;current$. In order to isolate the chloride current from background current, all known systems were blocked with specific blockers. The current-voltage relationship of caffeine-induced current showed outward rectification and it reversed at around $E_{Cl^-}$. The shift of reversal potential upon the alteration of external and internal chloride concentrations was well fitted with results which were calculated by the Nernst equation. Extracellular addition of N-phenylanthranilic acid and niflumic acid which are known anion channel blockers abolished the caffeine induced current. Intracellular application of a high concentration of EGTA also abolished this current. Application of c-AMP, c-GMP, heparin, or $AIF^-_4$ made no remarkable changes to this current. Sodium replacement with the impermeable cation N-methylglucamine or with $Cd^{2+}$ rarely affected this current. From the above results it is suggested that the caffeine induced current was a $Cl^-$ current and it was activated by intracellular $Ca^{2+}$.

• 한국포장학회:학술대회논문집
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• 2006.11a
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• pp.95-101
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• 2006

거창사과원예협동조합에서 구입한 사과 600상자를 5% $CaCl_2$ 용액에 각각 15분간 침지하는 방법으로 전처리 후 24시간 건조시키고 무포장구, $25{\mu}m$ 두께의 기능성 MA 필름으로 포장한 포장구와 무처리 무포장구인 대조구로 나누어 $0^{\circ}C$의 저온저장고로 옮겨 7개월 동안 저장하면서 3주 간격으로 품질을 분석하였다. 저장 기간이 증가할수록 중량 감소율은 Control 구보다 $CaCl_2$구와 $CaCl_2+MA$구의 중량 감소율이 월등히 낮았다. 경도변화는 저장 기간이 증가할수록 감소하는 경향을 보였으며, 그 중 $CaCl_2$구는 초기치 0.97 $kg_f$에서 저장 18주에는 0.95 $kg_f$로 초기치에 비해 2%의 가장 적은 변화를 보였다. 산도는 대조구보다 처리구의 산도가 높게 나타났으나 큰 차이는 나타나지 않았다. 가용성 고형분 함량은 저장 기간 모든 실험구에 따른 큰 차이가 없었다. 비타민 C 함량은 저장기간이 지날수록 모든 실험구에서 감소하는 경향을 보였으며, 기호도 조사에서 $CaCl_2$구와 $CaCl_2+MA$구가 대조구보다 유의적으로 높게 나타났다.

• Journal of the East Asian Society of Dietary Life
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• v.15 no.2
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• pp.219-225
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• 2005

Effect of blanching and/or $CaCl_2$ addition on the quality characteristics of Oiji prepared by dry salting method, which has been used for industry, was investigated. Control$(15\%\;salt),\;CAO(15\%\;salt\;+\;0.04\%\;CaCl_2)$ and BCO $(15\%\;salt\;+\;0.04\%\;CaCl_2+blanching\;at\;60^{\circ}C\;for\;20\;min)$ for 165 days. Acidity was lower, but pH was higher in BCO than in the control or CAO. During the whole period of storage, greenness(-a) of BCO was maintained in Hunter color system, compared with the other groups. The numbers of total microbes, lactic acid bacteria and yeast were the lowest in BCO, while the highest in control. Moreover, texture profile analysis exhibited that fracturability and hardness maintained the highest in BCO during storage, compared with the other groups. Based on these results, combination of blanching and $CaCl_2$ addition is favorable to extend the shelf life and to maintain the good quality.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.6
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• pp.1292-1295
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• 2006

Three kinds of electrolyte membranes were prepared by impregnating filter papers with one of the electrolyte solutions fur primary manganese battery ($NH_4Cl$, $ZnCl_2$, and alkaline types) and hygroscopic agent ($CaBr_2$ or $CaCl_2$), respectively. The thickness of them was $250{\sim}300{\mu}m$, and they were very flexible. The electrochemical characteristics greatly depended on the hygroscopic agent to supply water to the cell. The electrolyte membrane containing $CaCl_2$ showed the highest ionic conductivity and the largest discharge capacity.

• Korean Journal of Agricultural Science
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• v.6 no.2
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• pp.185-191
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• 1979

Compressive and tensile strengths of commonly being used mortar and mortar specially hardened by adding 1%, 2% and 3% of $CaCl_2$ were compared under the different mixing ratio of mortar and ages to investigate the effect of $CaCl_2$ which is being used as a promotor for coagulation and hardness, on the strength of mortar. The results obtained were as follows: 1. The compressive strength of mortar hardened by addition of $CaCl_2$ was higher than that of commonly being used mortar. The highest compressive strength of mortar was obtained when 2% of $CaCl_2$ were added. 2. The tensile strength of mortar, which was made by adding $CaCl_2$ and aged for seven days, was higher than that of commonly being used mortar. The highest tensile strength of mortar was obtained when 1% of $CaCl_2$ was added and aged for 28 days. And the tensile strength of mortar with 2% of $CaCl_2$ was lower than that of commonly being used mortar. 3. When the amount of $CaCl_2$ added was higher than 3%, the mortar was abruptly hardened and thereby occurred crack was considered lowering strength of mortar. 4. The rich mix was effective for the increasing the compressive and tensile strength before seven days of age and less effective after seven days of age. Therefore, the addition of one to two per cent of $CaCl_2$ would be effective for promoting initial strength of mortar during winter season.

• Geomechanics and Engineering
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• v.25 no.3
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• pp.245-251
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• 2021

The present study investigated the ageing effect on compressibility, permeability and shear strength behavior of kaolin and bentonite samples in the presence of NaCl and CaCl₂ solutions. The compressibility, permeability and shear strength parameters were determined on the 60, 190, and 250 days cured samples. The results have shown that, the kaolin sample becomes more compressible in the presence Ca²⁺ ions with ageing. Generally, the normalized compression index values of bentonite samples increased at the end of 60 days and 250 days curing time periods. The normalized permeability value of kaolin decreased by ageing in the presence of Na⁺ ions almost twofold. The permeability values of bentonite increased both in NaCl and CaCl₂ solutions during ageing. In the presence of Na⁺ ions kaolin had higher max. shear stress value than Ca²⁺ ions. When the max. shear stress values of 0, 60 and 190 days samples were compared, it was seen that NaCl solution had no significant effect on the shear strength of kaolin sample. However, the shear strength of kaolin increased in the CaCl₂ solution during ageing. In the presence of Ca²⁺ ions the max. shear stress value of bentonite was higher. The results of this study have shown that ageing has significant effects on the compressibility, permeability and shear strength of kaolinitic and bentonitic clayey soils.

• Current Research on Agriculture and Life Sciences
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• v.10
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• pp.147-155
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• 1992

This study was conducted to get basic information for factors affecting protoplast fusion between Petunia hybrida 'Titan red' and Nicotiana sanderae. The experiments such as fusogen, time of PEG treatment, temperature at fusion, $CaCl_2{\cdot}2H_2O$ concentration in fusion solution, and $CaCl_2{\cdot}2H_2O$ concentration and pH in eluting solution were carried out to increase the fusion efficiency. The results obtained were as follows; Fusion between P. hybrida and N. sanderae was accelerated when the mixture of the protoplasts was treated with 30% PEG 6,000 solution containing 5.5 mM $CaCl_2{\cdot}2H_2O$ for 10 minutes at $25^{\circ}C$, and subsequently eluted with a eluting solution containing 50 mM $CaCl_2{\cdot}2H_2O$ adjusted to pH 9.0.