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Plant Regeneration through Somatic Embryogenesis from Mature Zygotic Embryos of Ginseng(Panax ginseng C. A. Meyer) and Flowering of Plantlets (인삼(Panax ginseng C. A. Meyer)의 성숙한 배로부터 체세포 배발생을 통한 구분화 및 유식물체의 개화)

  • 이행순
    • Journal of Plant Biology
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    • v.32 no.3
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    • pp.145-150
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    • 1989
  • Mature zygotic embryos dissected from ginseng(Panax ginseng C. A. Meyer) seeds were cultured on Murashige and Skoog's (MS) medium containing various concentrations of 2, 4-dichlorophenoxyacetic acid(2, 4-D) and kinetin. Somatic embryos were induced directly from cotyledonary tissue or from intervening callus. The induction frequency of somatic embryos was up to 55%. Upon transfer to half-strength MS medium supplemented with 1 mg/1 6-benzyladenine(BA) and 1 mg/1 GA3, most somatic embryos developed into plantlets. Over 50% of the plantlets flowered after 4 weeks of culture and then a few bore immature fruits in vitro. Therefore, it is suggested that the juvenility of the ginseng tissue which give rise to somatic embryos does not interfere with in vitro flowering of their regenerated plantlets.

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Germination Percentage and Speed under International Seed Testing Association Conditions of Main Herbaceous Plants Used on the Slope (국제종자검정협회(ISTA) 변온조건에서 비탈면 복원용 주요 초화류의 발아율 및 발아속도)

  • Park, Jin-A;Shim, Sang-Ryul
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.21 no.3
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    • pp.47-56
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    • 2018
  • Research was initiated to investigate germination characteristics and germination pattern of 14 herbaceous plant entries used on the road slope during 30 days. An alternative germination condition for 14 herbaceous plant entries required by International Seed Testing Association(ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$. Significant differences were observed in the first germination rate(0.3%~40.7%)and in the final germination rate(7.7%~93.3%). Days to the first germination(2~8days), days to the 30% germination(2~6days) and days to the peak germination(6~18days) were different among 14 herbaceous plant entries in the study. From this result, we could find out higher final germination rate of 14 herbaceous plant entries in the following order; First, forage crops and cool-season turfgrasses; Second, herbeceous flowers; Third, wild plants. We could also divide germination rate among 14 herbaceous plant entries as 6 groups(I;very high, II;high, III;medium high, IV;medium low, V;low, VI;very low) based on the final germination rate and divide germination speed as 5 groups(A;very fast, B;fast, C;normal, D;slow, E;very slow)based on days to the peak germination. Considering germination characteristics and pattern of 14 herbaceous plant entries Medicago sativa, Lolium perenne, Festuca arundinacea and Cosmos sulphureus were regard as dominating species while Lespedeza cuneata, Silene armeria, Lotus corniculatus var.japonicus, Coreopsis tinctoria and Centaurea cyanus as competitive species following dominating species. However, Chrysanthemum burbankii, Pennisetum alopecuroides, Chrysanthemum boreale., Artemisia princeps var. orientalis and Arundinella hirta were not almost expected to emerge.

Experimental Study on Combined Ocean Thermal Energy Conversion with Waste Heat of Power Plant

  • Jung, Hoon;Jo, Jongyoung;Chang, Junsung;Lee, Sanghyup
    • KEPCO Journal on Electric Power and Energy
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    • v.5 no.3
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    • pp.215-222
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    • 2019
  • This work is experimental study of 10 kW specialized Combined Ocean Thermal Energy Conversion. We propose a C-OTEC technology that directly uses exhaust thermal energy from power station condensers to heat the working fluid (R134a), and tests the feasibility of such power station by designing, manufacturing, installing, and operating a 10 kW-pilot facility. Power generation status was monitored by using exhaust thermal energy from an existing power plant located on the east coast of the Korean peninsula, heat exchange with 300 kW of heat capacity, and a turbine, which can exceed enthalpy efficiency of 45%. Output of 8.5 kW at efficiency of 3.5% was monitored when the condenser temperature and seawater temperature are $29^{\circ}C$ and $7.5^{\circ}C$, respectively. The evaluation of the impact of large-capacity C-OTEC technology on power station confirmed the increased value of the technology on existing power generating equipment by improving output value and reducing hot waste water. Through the research result, the technical possibility of C-OTEC has been confirmed, and it is being conducted at 200 kW-class to gain economic feasibility. Based on the results, authors present an empirical study result on the 200 kW C-OTEC design and review the impact on power plant.

Studies of Physiological Action of Chemicals to Increase in Ripening of Rice Plant II. Investigation of Chemical Effect and Ripeness of Rice Plant (수도등숙 향상을 위한 생리생태연구 제2보 ABA 및 BA처리가 수도등숙에 미치는 영향에 대하여)

  • Seo, G.S.;Lee, J.Y.;Kim, S.Y.;Ota,Yasuo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.2
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    • pp.189-194
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    • 1983
  • The experiment was carried out to know the ripeness effect of Gemgang when ABA and BA were sprayed at the heading stage. ABA promoted the stomatal movement, BA kept plant from senescence. Percent of filled grain, grain weight, photosynthesis, content of chlorophyll, transpiration and content of ATP were measured at 1-week interval from 2-weeks after heading.

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Effect of Medicinal Plant Extracts on Apple Storage Diseases (약용식물 추출물에 의한 사과 저장병 방제 효과)

  • 백수봉;정일민
    • Korean Journal Plant Pathology
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    • v.13 no.1
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    • pp.57-62
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    • 1997
  • This experiment was conducted to test the control effect of methanol extracts of 10 medicinal plants on apple storage diseases caused by Botryosphaeria berengeriana, Glomerella cingulata and Penicillium expansum. Out of the 10 medicinal plants, methanol extracts of Coptis japonica and Anemarrhena asphodeloides inhibited effectively the mycelial growth of B. berengeriana, G. cingulata and P. expansum in vitro, for which the inhibition ratios of the two plant extracts were 100.0% and 89.3%, 73.7% and 94.1%, and 100.0% and 51.6%, respectively. Spore germination of the three fungi was inhibited 100% only by C. japonica extract, but only P. expansum was inhibited 100% by A. asphodeloides extract. No lesion was formed y the fungi at 5$^{\circ}C$ up to 2 weeks after inoculation. Lesion sizes produced by the three fungi at the temperature ranges of 1$0^{\circ}C$ to $25^{\circ}C$ and infection of B. berengeriana and G. cingulata were inhibited by C. japonica extract, but not by A. asphodeloides extract, while no lesion was formed by the fungi at 5$^{\circ}C$. Infections of the fungi on apples were somewhat stimulated by A. asphodeloides extract.

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Analysis on the Structure of Plant Community in Mt. Yongmun by Classification and Ordination Techniques (Classification 및 Ordination 방법에 의한 융문산 삼림의 식물군집 구조분석)

  • 이경재
    • Journal of Plant Biology
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    • v.33 no.3
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    • pp.173-182
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    • 1990
  • To investigate the structure of the plant community structure of Mt. Yongmun in Kyonggi-do, fifty-four plots were set up by the clumped sampling method. The classification by TWINSPAN and DCA ordination were applied to the study area in order to classify them into several groups based on woody plant and environmental variables. By both techniques, the plant community were divided into two groups by the aspect. the dominant species of south aspect were Pinus densiflora, Quercus aliena, Q. mongolica, Carpinus laxiflora and of north aspect were Q. ongolica, Fraxinus rhynchophylla. The successional trends of tree species in south aspect seem to be from P. densiflora through Q. serrata, Q. aliena, A. mongolica to C. laxiflora. As a result of the analysis for the relationship between the stand scores of DCA and environmental variables, they had a tendency to increase significantly from the P. densiflora and Q. mongolica community to C. laxiflora and F. rhynchophylla community that was the soil moisture, the amount of soil humus and soil pH.

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Effect of Forcing Date and Temperature on Growth and Flowering of Iris koreana and Iris minutoaurea (노랑붓꽃과 금붓꽃의 입실시기 및 온도가 생장 및 개화에 미치는 영향)

  • Lee, Jeong Ho;Lee, Chung Hee;Park, Gwang Woo;Song, Cheon Young
    • Journal of Korean Society of Forest Science
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    • v.96 no.6
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    • pp.699-704
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    • 2007
  • This experiment was conducted to investigate growth and flowering characteristics of Iris koreana and Iris minutoaurea Makino native to Korea for potting plant of early spring treated by forcing date and temperature. As forcing date delayed from November 1 to December 1, the plant height and number of leaves were increased, however no difference in the number of flower and date of flowering. In Iris koreana, the plant was not flowering in forcing on November 1, flowering 30% on November and 100% on December 1. However all the forcing plant flowered in Iris minutoaurea Makino. As forcing temperature increased from $7^{\circ}C$ to $15^{\circ}C$, the plant height and number of leaves were decreased, however the number of flower in $7^{\circ}C$ was increased and date of flowering was earlier than ones of higher temperature. In Iris koreana, the plant was not flowered in forcing $12^{\circ}C$ and $15^{\circ}C$, all flowered in forcing $7^{\circ}C$. Iris minutoaurea Makino was flowered 30% forcing in $12^{\circ}C$ and all flowered forcing in $7^{\circ}C$.

A Newly Known Genus Charitoprepes Warren (Lepidoptera: Pyraloidea: Crambidae) in Korea, with Report of C. lubricosa Warren (한국산 Charitoprepes 속 (나비목: 명나방상과: 포충나방과)의 1 미기록종 보고)

  • Kim, Minyoung;Park, Young-Mi;Hyun, Ik-Hwa;Kang, Byoung-Hyo;Oh, Si-Heon;Jwa, Jae-Kwang;Hyun, Young-Kwon;Lee, Heung-Sik
    • Korean journal of applied entomology
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    • v.53 no.3
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    • pp.301-303
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    • 2014
  • The genus Charitoprepes (Warren), a probable vagrant group of the family Crambidae is newly recorded for the first time from the Korean Peninsula, which was described based on C. lubricosa (Warren) from Jeju islands. Diagnosis and illustrations of detailed diagnostic characters, including genitalia are provided.

High Concentration of Sodium Chloride Increases on Survival of Non-pathogenic Erwinia carotovora subsp. carotovora 9-3 during Drying and Storage (비병원성 Erwinia carotovora subsp. carotovora 9-3의 건조 및 저장의 과정에서 생존에 미치는 염 효과)

  • Park, Kyoung-Soo;Kim, Gun-Ju;Shin, Yun-Ju;Kim, Sik;Cha, Jae-Soon
    • The Korean Journal of Pesticide Science
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    • v.12 no.4
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    • pp.368-374
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    • 2008
  • Dry formulation is a limiting step for successful development of microbial bio-pesticides with the antagonistic Gram-negative bacteria because their survival rates are too low during drying and storage. The high concentration of sodium chloride (NaCl) in culture medium that induces osmolyte in bacterial cells is known to increase of survival rate during drying in many Gram-negative bacteria. Effect of NaCl on survival of antagonistic non-pathogenic Erwinia carotovora subsp. carotovora 9-3 (Ecc 9-3) during drying and storage was studied. Growth rate of Ecc 9-3 was not much different up to 0.5 M NaCl in NB while it was lower significantly with 0.7 M NaCl. Survival rates were twice and 3 times higher with 0.5 M NaCl than with no additional NaCl during drying at room temperature and freeze-drying, respectively. Survival rate was also higher with high NaCl in culture medium during storage after drying. It was not much different on storage at $4^{\circ}C$ both of drying at room temperature and freeze-drying. However, the survival rate was higher on storage at $27^{\circ}C$ and $37^{\circ}C$ with high NaCl concentration. Among the additives tested, lactose affects most to survival rate both of drying at room temperature and freeze-drying, and dextrin influenced significantly to survival rate of drying at room temperature.