• Plant Resources
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• 제8권3호
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• pp.263-274
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• 2005

We have studied the effects of dampening papers (Dampened paper, Newspaper, Korean paper, Flower sheet), silica gel (30g, 60g) and temperature ($20^{\circ}C,\;25^{\circ}C$) on color changes of Erythronium japonicum. In the treatment of $20^{\circ}C$, color changes were low in treatments with silica gel rather than in a treatment of dampening papers. In particular, newspaper and Korean paper showed much less changes in colors by the combination treatments with 30g of silica gel. Likewise, in the treatment of $25^{\circ}C$, color changes were low in combination treatments with silica gel rather than in a alone treatment of dampening papers. For the combination treatment with 30g of silica gel, low color changes were shown in the divisions of newspaper and Korean paper, while for the combination treatment with 60g of silica gel, low color changes were shown in the those of Korean paper and dampened paper.

• 환경위생공학
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• 제21권3호
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• pp.37-43
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• 2006

Recently, needs for reuse of sludge produced from WTP(water treatment plant) have been increased with shortage of landfill sites and difficulties of the treatment and disposal processes. Therefore, Reusing is becoming an Increasingly popular waste management alternative to divert waste from landfills. In order to research the characteristics of WTP sludges, we used the sludges of C WTP which intake the lake Dae-Cheong and the sludges of S WTP which intake Keum river, The specific surface area of C and S WTP sludges were $0.9986m^2/cc\;and\;1.874m^2/cc$, respectively. The gravity was about $2.0{\sim}2.4$ which are scope of peat or loamy clay. The major minerals of C WTP sludges were kaolinite(48.4%), muscovite(19.5%), and quartz(16.7%). Also, muscovite(31.6%), quartz(30.3%), and kaolinite(17.3%) in S WTP sludges were major minerals.

• Molecules and Cells
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• 제23권1호
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• pp.80-87
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• 2007

Beet curly top virus (BCTV) and Beet severe curly top virus (BSCTV), members of curtoviruses, encode seven open reading frames (ORFs) within a ~3 kb genome. One of these viral ORFs, C1, is known to play an important role in the early stage of viral infection in plants during initiation of viral DNA replication. We used promoter:: reporter (${\beta}$-glucuronidase) gene fusions in transgenic Arabidopsis to identify the putative promoter region of BCTV ORF C1. Unlike other geminiviruses, the intergenic region of BCTV was not sufficient to promote C1 expression in transgenic plants. When sequences extending into the coding region of C1 were tested, strong expression of the reporter protein was observed in vascular tissues of transgenic plants. This expression was not dependent on the presence of the intergenic regions or proximal 5' portions of the C1 coding region. Transgenic plants expressing a reporter gene under control of the putative complete C1 promoter were inoculated with virus to determine if any viral transcript affected C1 expression. Virus inoculated plants did not show any altered pattern or change in of reporter gene expression level. These results suggest that (1) important transcriptional activator elements for C1 expression reside in the 3' portion of C1 coding area itself, (2) C1 protein does not auto-regulate its own expression and (3) C1 expression of two curtoviruses is controlled differently compared to other geminiviruses.

• 한국작물학회지
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• 제29권3호
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• pp.232-241
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• 1984

수도 약배양의 효율을 높이기 위하여 callus 유기와 녹색체 출현율에 영향하는 화분의 발육단계, 화분의 저온처리와 기관 및 배지의 영향에 대한 일련의 실험을 수행하였으며 이에서 얻어진 결과를 요약하면 다음과 같다. 1. 단핵기의 화분에서 callus 유기율이 높았으며 이때는 대체로 엽신간장이 5~8cm에 해당되나 품종에 따라 차이가 있다. 2. 이삭을 상, 중, 하로 나누어 조사한 결과 엽이간장이 5~8cm의 것은 상부와 중간부분에 단핵기에 해당되는 것으로 나타났고 9~10cm 정도의 것은 중부와 하부 부분이 단핵기에 해당하는 것으로 나타났다. 3. 일반적으로 저온처리는 8$^{\circ}C$에서 12$^{\circ}C$가 callus 유기에 알맞는 것으로 나타났다. 그러나 온도에 대한 반응은 품종에 따라 차이가 있어 4$^{\circ}C$에서 callus 유기가 잘된 것도 있었다. 4. 저온 처리기간은 일반적으로 8~12일 정도에서 callus 유기가 잘 되었으나 품종간 차이가 있다. 5. 본 실험에서는 N6D 배지보다 N6 배지에서 callus 유기율이 높았다. 6. 대체로 녹색체 출현율은 4$^{\circ}C$와 8$^{\circ}C$로 처리한 것이 12$^{\circ}C$ 처리보다 높았다. 그러나 품종간 차이가 있었다. 7. 녹색체 출현에 좋은 결과를 주었던 온도 처리기간은 대체로 8~12일로 나타났다. 8. 본 실험에서 녹색체 출현율은 N6 배지보다 개량 MS 배지에서 2배 정도 높았다.

• 한국가금학회지
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• 제21권3호
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• pp.183-193
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• 1994

White Leghorn hens were fed a commercial formula feeds as a basal diet, which was supplemented with 3 types of plant oil safflower, evening primrose and pine seed, at the 10% level for 3 weeks. No significant changes was found between the basal diet and the oil feeding trials in the egg yolk cholesterol content, the laying rate, the egg weight, and the yolk weight, except the feed intake. In the fatty acid composition of the egg yolk lipid, however, the plant oil feedings to hens resulted in considerable increase of $C_{18:2}$ acid with a simultaneous decrease of $C_{18:2}$ acid ,and, consequently, followed by the improvement of balance with P /S and P /M /S ratio close to 1.0 and 1:1:1, which is known a desirable ratio for human health in lipid nutrition, respectively.

• 한국식물병리학회지
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• 제11권3호
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• pp.258-264
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• 1995

Production and some properties of chitinolytic enzymes were investigated by 80% ammonium sulfate precipitates (crude enzymes) from culture supernatant of antagonistic bacteria, Chromobacterium violaceum strain C-61 and strain C-72, Aeromonas hydrophila, Aeromonas caviae, and Serratia marcescens. The maximum production of chitinase was obtained from the 3-day culture at 28$^{\circ}C$ in C. violaceum stains, the 6-day culture in S. marcescens, and the 2-day culture in A. hydrophila and A. caviae. In the optimum culture periods, chitinase activity of C. violaceum strains C-61 was 1.5, 5.5, 12.0 and 11.3 times higher than those of strain C-72, S. marcescens, A. hydrophila and A. caviae, respectively. However, N,N'-diacetylchitobiase activity was 3.2 times higher in S. marcescens than in C. violaceum strain C-61, and that of Aeromonas spp.was very low. On gels containing glycol chitin, chitinase of C. violaceum strains showed four isoforms of 54-, 52-, 50- and 37-kDa, whereas there were four isoforms of 58-, 52-, 48- and 38-kDa in S. arcescens, three isoforms of 70-, 58- and 54-kDa in A. hydrophila and six isoforms of 90-, 79-, 71-, 63-, 58- and 38-kDa in A. caviae. The chitinase of C. violaceum strain C-61 was most active at pH 7.0 and at 5$0^{\circ}C$ and was stable in ranges of pH 5.0~10.0 for 2 hours and of 0~5$0^{\circ}C$ for 30 min.

• The Plant Pathology Journal
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• 제39권4호
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• pp.384-396
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• 2023

Colletotrichum acutatum species complex is one of the most important groups in the genus Colletotrichum with a high species diversity and a wide range of host plants. C. acutatum and related species have been collected from different plants and locations in Korea and deposited into the Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Sciences since the 1990s. These fungal isolates were previously identified based mainly on morphological characteristics, and a limitation of molecular data was provided. To confirm the identification of species, 64 C. acutatum species complex isolates in KACC were used in this study for DNA sequence analyses of six loci: nuclear ribosomal internal transcribed spacers (ITS), betatubulin 2 (TUB2), histone-3 (HIS3), glyceraldehyde3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), and actin (ACT). The molecular analysis revealed that they were identified in six different species of C. fioriniae (24 isolates), C. nymphaeae (21 isolates), C. scovillei (12 isolates), C. chrysanthemi (three isolates), C. lupini (two isolates), and C. godetiae (one isolate), and a novel species candidate. We compared the hosts of KACC isolates with "The List of Plant Diseases in Korea", previous reports in Korea and global reports and found that 23 combinations between hosts and pathogens could be newly reported in Korea after pathogenicity tests, and 12 of these have not been recorded in the world.

• The Plant Pathology Journal
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• 제37권1호
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• pp.79-85
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• 2021

Chemical and biological agents were evaluated to inhibit Colletotrichum fructicola, Phytophthora cactorum, and Lasiodiplodia theobromae causing strawberry diseases. Mycelial growths of C. fructicola were gradually arrested by increasing concentrations of fungicides pyraclostrobin and iminoctadine tris (albesilate). P. cactorum and L. theobromae were more sensitive to pyraclostrobin compared to C. fructicola, but iminoctadine tris (albesilate) was not or less effective to limit P. cactorum or L. theobromae, respectively. Bacillus siamensis H30-3 was antagonistic against the three pathogens by diffusible as well as volatile molecules, and evidently reduced aerial mycelial formation of P. cactorum. B. siamensis H30-3 growth was declined by at least 0.025 mg/ml of pyraclostrobin. The two fungicides additively inhibited mycelial growths of C. fructicola, but not of P. cactorum and L. theobromae. B. siamensis H30-3 volatiles led to less growth of C. fructicola than one reduced by the fungicides. Taken together, in vitro antimicrobial activities of the two fungicides together with or without B. siamensis H30-3 volatiles may be cautiously incorporated into integrated management of strawberry diseases dependent on causal pathogens.

• 한국자원식물학회지
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• 제35권2호
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• pp.380-384
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• 2022

본 연구에서 PTS는 지방전구세포에서 PPARγ, C/EBPα 및 FABP4의 발현 억제를 통해 지방전구세포의 adipogenesis를 억제하고, AMPK 및 HSL의 활성화, ATGL의 발현증가, 그리고 perilipin-1의 발현감소를 통해 지방세포의 지질을 분해하여 지방세포 내 과도한 지질형성을 억제하는 것으로 판단된다. 이러한 결과들은 PTS는 비만 예방 및 치료를 위핸잠재적 식의약 소재로의 개발 및 활용이 가능할 것으로 사료된다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 추계학술대회 및 한일 식물생명공학 심포지엄
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• pp.3-8
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• 2005

Torenia hybridacv. Summerwave Blue and Violet mainly produce delphinidin. Down regulation of their flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase (F3'5'H) genes and over expression of rose or pelargonium dihydroflavonol 4-reductase (DFR) cDNA yielded pelargonidin-based bright pink flowers. Nierembergia cv. Fairybells lack pink color as they produced only delphinidin and flavonols. Pelargonidin-based pink flowers were achieved by down regulation of F3'5'H and flavonol synthase genes and over expressing rose DFR cDNA. Introduction of petunia F3'5'H and DFR cDNAs into white carnations deficient in DFR activity produced violet carnations, which arc now commercialized in the USA, Canada, Australia, Europe and Japan. Introduction of pansy F3'5'H and iris DFR cDNAs and down regulation of rose DFR gene produced rose flowers which accumulates delphinidin imparting novel violet color.