• Title/Summary/Keyword: $C_{12}A_7$

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Effects of Temperature on the Activity of Pulmonary Surfactant of the Rabbit (온도(溫度)가 가토(家兎) 폐포표면(肺胞表面) 활성물질(活性物質)의 활성도(活性度)에 미치는 영향(影響))

  • Kwon, Koing-Bo
    • The Korean Journal of Physiology
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    • v.7 no.2
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    • pp.1-8
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    • 1973
  • Though it has been reported by Clements et al. and Avery et al. that the activity of the pulmonary surfactant can be altered by the temperature changes, a conclusive evidence of the effects of temperature on the surfactant system of the lung is yet to come. In the present study, an attempt was made to observe possible effects of a few different degrees of temperature on the activity of the pulmonary surfactant of the rabbit in vivo and in vitro. The rabbit was sacrificed by blood shedding and both lungs were completely removed. The lung washings, obtained by gently lavaging the left lung with saline, was placed at 1) 4C for 1, 5, 10, 15, 30 and 40 days, and 2) 20C for 1, 2, 3, 4, 5 and 7 days for in vitro experiment. For in vivo experiment, the rabbit was placed at 4C for 4, 8, 12 and 24 hours, and the lung lavage was prepared as described above in the in vitro experiment. Tension-area (T-A) diagram of the lung lavage was recorded automatically by a modified. Langmuir-Wilhelmy balance with a synchronized recording system. The surface tensions thus obtained were compared with those of the normal rabbit, and the results are summarized as follows: 1. The maximal surface tension, minimal surface tension and stability index of the normal rabbit lung lavage were $52.5{\pm}2.3\;dynes/cm,\;4.9{\pm}2.3\;dynes/cm$ and 1.65, respectively. 2. In the group where the lung lavage was placed at 4C in vitro, the maximal and minimal surface tensions, and stability index did not show any noticeable changes comparing with the normal values up to 30 days. On the 40th day of the experiment, a tendency of a slight increase in the surface tensions was observed but the change was not significant. 3. When the lung lavage was placed at 20 C in vitro, the maximal surface tension did not show any appreciable change comparing with the normal except on the 7 th day with a slight increase. The minimal surface tension showed an increased value from the 2nd day, and on the 5 th and 7 th experimental day, markedly increased value was observed. The stability index, on the other hand. showed a marked decrease throughout the entire experiment with the value of 0.71 and 0.53 on the 5th and 7 th day, respectively. 4. In the group where the rabbit was placed at 4 C in vivo, the maximal surface tensions and stability index of the lung lavage showed little change from the normal. The minimal surface tension at 12 experimental hour showed a slight increase, but it returned to the normal value at 24 hour.

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Frequencies and Distributions of Unexpected Antibodies at a General Hospital in the Daejeon of Korea (대전지역 일개 종합병원에서의 비예기 항체 발생현황과 분포)

  • Kim, Jae-Jung
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.3
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    • pp.354-358
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    • 2018
  • When preparing for a blood transfusion, the presence and types of unexpected antibodies should be identified through screening tests. Using the DiaMed-ID system, antibody identification among unexpected antibody screening tests performed at a university hospital in Daejeon area for two years from January 2016 to December 2017 were limited to 55 patients and were predominantly women. A total of 36 patients (65.5%) belonged to the Rh group, 7 (12.7%) patients to the Lewis group, 4 (7.3%) patients to the Kidd and Duffy groups, 3 patients (5.5%) to the MNS group, and 1 (1.8%) to the Rh+Kidd combined group. In the Rh group, 19 (34.5%) patients had Anti-E single antibody, 5 (9.1%) patients had Anti-D single antibody, 4 (7.3%) patients had Anti-E/-c, 4 (7.3%) patients had Anti-C/-e, and 1 (1.8%) patient had $Anti-E/-c/-Jk^b$. In the Lewis group, three (5.5%) patients had both $Anti-Le^a$ and $Anti-Le^b$. In the Kidd group, one (1.8%) patient had $Anti-Jk^a$ and three (5.5%) patients had $Anti-Jk^b$. In the Duffy and MNS groups, only single antibody was found: one (1.8%) patient with $Anti-Fy^a$, three (5.5%) patients with $Anti-Fy^b$, two (3.6%) patients with Anti-M, and one (1.8%) patient with Anti-S. This study reflects the recent frequencies and distributions of unexpected antibodies in Daejeon, which would be helpful for the efficient preparation for transfusions.

Purification and Characterization of Cyclodextrin Glucanotransferase from Paenibacillus sp. JK-12

  • Kang, Yong;Kim, Sung-Koo;Jun, Hong-Ki
    • Preventive Nutrition and Food Science
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    • v.7 no.3
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    • pp.310-316
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    • 2002
  • Extracellular cyclodextrin glucanotransferase (CGTase) from Paenibacillus sp. JK-12 was purified through sev-eral purification steps consisting of ammonium sulfate precipitation and chromatographies on DEAE-sephadex A-50 and Mono QIM HR5/5. The purified CGTase exhibited a single band on SDS-PAGE and was estimated to be approximately 82 kDa. The isoelectric point of the enzyme was 7.2 as determined by isoelectric focusing. The CGTase from Paenibacillus sp. JK-12 had a transglucosylation activity at the C-2 position of L-ascorbic acid. The optimum pH and temperature for the CGTase activity were 8.0 and 5$0^{\circ}C$, respectively. The enzyme activity was stable from pH 6.0 to 9.() and at temperatures up to 55$^{\circ}C$ at pB 8.0, having 80% residual activity. The activity of the CGTase was strongly resistant to metals such as A $g^{+}$ and $Ba^{2+}$ but slightly inhibited by H $g^{+}$, N $i^{2+}$ and $Mg^{2+}$. The enzymeproduced $\alpha$ -cyclodextrin ($\alpha$-CD) and $\beta$-CD as the main products from starch, but not ${\gamma}$-CD.X>-CD.

Multiplication and Survival of Salmonellae in Raw Meat (Salmonella균(菌)의 생육내(生肉內) 증식(增殖) 및 생존(生存))

  • Chung, G.T.
    • Korean Journal of Veterinary Research
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    • v.8 no.1
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    • pp.1-5
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    • 1968
  • Multiplication and survival of S. typhhi-murium S. anatum and S. meleagridis in raw meat were observed at different storage temperatures. All the three serotypes grew in raw meat at $10^{\circ}C$ reaching the maximum numbers in three to five days. However, none of the serotypes grew at $7^{\circ}C$ or below. At both 7 and $5^{\circ}C$ the number of organisms remained almost constant for the first few weeks but decreased slightly thereafter At $-12^{\circ}C$ the numbers of S. typhi-murium and S. anatum decreased very slowly over a period of six month but the number of S. meleagridis decreased faster than the other two serotypes.

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Measurement of elastic constants of single crystal PbMoO_4$ by using brillouin scattering experiment (브릴루앙 산란실험을 이용한 단결정 PbMoO_4$의 탄성계수 측정)

  • 박주일;이석목;유윤식;김성철
    • Korean Journal of Optics and Photonics
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    • v.7 no.4
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    • pp.363-369
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    • 1996
  • We have performed Brillouin scattering experiments to investigate the elastic properties of tetragonal symmetry single crystal $PbMoO_4$ and could determine the value of birefringence as well as the whole elastic constants. As a result, $c_11=111.4{\pm}4.4,$$, $c_12=64.7{\pm}3.4$, $c_44=27.0{\pm}0.8$, $c_13=51.9{\pm}2.5$, $c_33=95.5{\pm}1.9$, $c_66=34.5{\pm}4.6$ and $c_16=15.8{\pm}1.2({\times}10^9N/m^2)$ and $n_o-n_e=0.151{\pm}0.018$.

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An Analysis Method on Injury Symptoms Utilizing Infrared Thermal Imaging under the Freezing Stress of Hedera helix L. (헤데라 헬릭스 식물의 적외선 열영상에 의한 저온 및 한풍피해에 관한 연구)

  • Seong, Bu-Geun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.6
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    • pp.173-179
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    • 2012
  • The experiments, which analyze the injury symptoms and diagnose growth conditions utilizing IRVT and analyzing each parts of H. helix L., had been held under a low temperature. Greenhouse and outdoor growing Genus hedera had been prepared and compared with each Genus hedera's peak and bottom leaves' surface temperature under the experimental categories $-6^{\circ}C$ and $-12^{\circ}C$. As results, analyzing the surface thermal property of peak part leaves' of outdoor growing Genus hedera, at experimental categories $-6^{\circ}C$, $-12^{\circ}C$ were ranged from $-2^{\circ}C{\sim}-7^{\circ}C$ and $-2^{\circ}C{\sim}-15^{\circ}C$. On the other hand, the surface thermal property of bottom part leaves at experimental categories $-6^{\circ}C$, $-12^{\circ}C$ were ranged $-2^{\circ}C{\sim}-11^{\circ}C$ and $-1^{\circ}C{\sim}-12^{\circ}C$. It appears that the thermal properties of leaves' surface on $-6^{\circ}C$ peaks and $-12^{\circ}C$ bottoms were more broadband than bottoms and peaks. It means that the peaks were more sensitive than bottoms, as like $-2^{\circ}C{\sim}-15^{\circ}C$, $-1{\sim}-12^{\circ}C$. Moreover, as similar results had seen to leaves surface temperature added to cold wind conditions. How the cold wind damaged the outdoor growing Genus hedera, analyzed the surface thermal property by IRVT data under $0^{\circ}C$, $-2^{\circ}C$, $-4^{\circ}C$ condition, it resulted to $-6.2^{\circ}C$, $-6.8^{\circ}C$, $-7.5^{\circ}C$. It appeared more $3.5{\sim}6.2^{\circ}C$ low temperature than experimental setting point. In addition, each parts thurmal property of peaks and bottoms was not similar, it referred to each parts' sensitivities of low temperature were different on the peak and bottom leaves surface temperature.

Effects of 2-Chloroethylphosphnic Acid on Ripening of Tobacco Leaves (2_Chloroethylphosphonic Acid가 잎담배 성숙에 미치는 영향)

  • Il Hou;Han-Seo Koo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.12
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    • pp.71-75
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    • 1972
  • To investigate the effects of C. E. P. A(2-chloroethyl phosphonic acid) on the ripening of tobacco leaf, the effects on the yield and quality of leaf tobacco, this experiments were carried out during the period of from 1970 to 1971 at tobacco experiment station, Sosa, Korea and 3 locations. The results are summarized as follows. 1. The higher the C. E. P. A concentration was, the more the leaf ripening was accelerated. During the period from 3 to 4 days after treatment, the differences of leaf ripening among levels were prominent. 2. Treatment with C. E. P. A only on the upper surface of the tobacco leaf, accelerated the ripening of that particular part treated, but not apparently the other parts of leaf. 3. Distinctive acceleration of leaf ripening was, obserbed in the fully develope1lower leans, however, the upper leaves were indistinctive. 4. The higher C. E. P. A concentration was, the more the effect of ripening acceleration was. But the yield was reduced over 900ppm because of the low of growth of leaves and the reduced yield was 90% at the 3, 000ppm. So the proper concentration was regarded as 900ppm. 5. In the view point of the days of C. E. P. A ripening acceleration, it was shortened one days at 100 ppm, three days at 300ppm, three days at 450ppm, four days at 900ppm, seven days at 3, 000ppm. 6. In the point of curing process, it was possible that the curing time and fuel was reduced 29% and 45% respectively in the C. E. P. A treatment than the check. 7. Therefore, if it is treated the C. E. P. A at 900ppm in the tobacco cultivation, the quality shall be increased 13.5% and the price shall be increased 12% in the 10 Are. In the point of subsidiary affect, it is possible that the C. E. P. A ripening acceleration is shortened about 7 days at 3, 00ppm and curing time is shortened about 24 hours.

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Behaviour of Fungicide $^{14}C-Propiconazole$ in a Lysimeter of Sandy Loam (사양토성 Lysimeter에서 살균제 $^{14}C-Propiconazole$의 행방)

  • Kim, In-Seon;Suh, Yong-Tack
    • Applied Biological Chemistry
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    • v.41 no.3
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    • pp.253-257
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    • 1998
  • Behaviour of a fungicide $^{14}C-propiconazole$ was investigated in a field lysimeter of sandy loam soil. At 15 days after rice-seedling transplanting, $^{14}C-propiconazole$ was treated on the soil surface at the rate of 0.12 kg/10a. The cummulative $^{14}C-radioactivity$ in the leachate from the lysimeter soil was 4.4% of the applied $^{14}C$ for 16 weeks. Most radioactivity detected in leachate was in the form of parent compound. At the end of lysimeter experiment. $^{14}C$ radioactivity in lysimeter soil was 76.5% of the applied $^{14}C$ and more than 97% of $^{14}C$ in soil remained in the top 20 cm. The percent of $^{14}CO_2$ evolved from lysimeter soil was 7.8% of the applied $^{14}C$. The radioactivity remained in the rice straw after harvest was 7.2% of the applied $^{14}C$, while less than 0.1% of the applied $^{14}C$ was detected in flag leaf, ear, chaff and hulled rice, respectively.

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Effects of Carbide and Matrix Structures on Abrasion Wear Resistance of Multi-Component White Cast Iron (다합금계 백주철의 탄화물 및 기지조직이 내마모성에 미치는 영향)

  • Ryu, Seong-Geun
    • Korean Journal of Materials Research
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    • v.7 no.4
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    • pp.310-316
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    • 1997
  • The effects of carbide and matrix structures on the abrasion wear resistance of multi-component white cast irons with 3.0 mass%C have been studied in this paper. Four different heats were poured in order to obtain the specimens with different combinations of the carbide structures: a basic iron(3.0 mass%C-5.0 mass%Cr-5.0 mass%V-5.0 mass% Mo-12.5mass%W)for M$_{6}$C and M$_{7}$C$_{3}$ carbides, and a Cr free iron(3.0 mass%C-5.0 mass%V-2.5mass%Mo-12.5 mass%W) for MC and M6C carbides. A conventional high Cr free free iron(3.0 mass%C-5.0 mass%V-2.5 mass%Mo-12.5 mass%W) for MC and M6C carbides. A conventional high Cr white cast iron was also poured to compare its wear resistance with those of the multi-component white cast irons. In the as-cast condition, the range of abrasive wear rate(Rw=mg/min) was from 4.15 to 5.98 . The lowest Rw, which means the highest wear resistance, was obtained in the basic iron with nodular MC, lamellar M$_{2}$C and cellular M$_{7}$C$_{3}$ carbides. On the other hand, the Rw of the high Cr white cast iron ranked between the basic iron and the Mo and W free iron. In each alloy, the Rw of air hardened or tempered specimen was lower than that of the as-cast one because of the change of matrix structures by the heat treatments. The Rw of the hear treated speci-mens increased in the order Mo and W free iron, basic iron, Cr free iron, high Cr iron, and V free iron.n.n.n.

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HbA1c changes in patients with diabetes following periodontal therapy

  • Kim, Su-Hwan;Lee, Jihye;Kim, Won-Kyung;Lee, Young-Kyoo;Kim, Young-Sung
    • Journal of Periodontal and Implant Science
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    • v.51 no.2
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    • pp.114-123
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    • 2021
  • Purpose: This retrospective cohort study aimed to assess the effect of nonsurgical periodontal therapy on glycated hemoglobin (HbA1c) levels in patients with both type 2 diabetes and chronic periodontitis. Methods: The intervention cohort (IC) comprised 133 patients with type 2 diabetes who received nonsurgical periodontal treatment, while the matching cohort (MC) included 4787 patients with type 2 diabetes who visited the Department of Endocrinology and Metabolism of Asan Medical Center. The patients in each cohort were divided into 3 groups according to their baseline HbA1c level: subgroup 1, HbA1c <7%; subgroup 2, 7%≤ HbA1c <9%; and subgroup 3, HbA1c ≥9%. Changes in HbA1c levels from baseline to 6 and 12 months were analyzed. In addition, the association between changes in HbA1c levels and the number of periodontal maintenance visits was investigated. Results: There were no statistically significant changes in HbA1c levels in the IC and MC or their subgroups when evaluated with repeated-measures analysis of variance. However, the IC showed maintenance of baseline HbA1c levels, while the MC had a trend for HbA1c levels to steadily increase as shown by pairwise comparisons (baseline to 6 months and baseline to 12 months). IC subgroup 1 also maintained steady HbA1c levels from 6 months to 12 months, whereas MC subgroup 1 presented a steady increase during the same period. The number of periodontal maintenance visits had no association with changes in HbA1c levels during the 1-year study duration. Conclusions: For patients with both type 2 diabetes and periodontitis, nonsurgical periodontal treatment and periodontal maintenance may help to control HbA1c levels.