• Korean Journal of Soil Science and Fertilizer
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• v.46 no.3
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• pp.163-172
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• 2013

The aquatic insect collected at six areas (each 2 for mountain area, plain field, and urban area) from 2009 to 2011 were classified to analyze the distribution and diversity of species. Frequency (number of aquatic insect: N), number of species (S), similarity index (C), richness index (R1, R2), variety index (V1, V2), evenness index (E1, E2, E3, E4, E5), and dominance index (D1) were investigated. Total N and S were 143 and 84, respectively. C matrix of 153 combinations was constructed with the average of 0.542. The average C of 3 years (0.659) was 9.9% P, more higher than the average C of 6 areas (0.560). The average values of the index of 18 plots were 2.28, 0.17, 1.24, 1.08, 0.07, 0.06, 0.01, 0.87, 0.31, 0.93 for R1, R2, V1, V2, E1, E2, E3, E4, E5, D1, respectively. The order in the coefficient of variation (CV) of the indicator for 18 plots was N (70.0%) > E3 (54.9%) > E1 (49.6%) > R2 (40.5%) > S (35.3%) > R1 (33.7%) > E2 (28.4%) > E5 (15.9%) > V1 (11.1%) > E4 (6.3%) > V2 (5.1%) > D1 (4.8%). The correlation matrix with 66 combinations between the indexes was constructed with statistical significance for 33 combinations. However, R1, V1, E2 and D1 were the proper indexes to represent species diversity of aquatic insect based on the correlation matrix and the theory of statistical independence. The richness index was highest in mountain, variety index in urban area, and evenness index in plain field. However, the dominance index was lowest in urban area.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.1
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• pp.55-59
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• 2000

A specific and sensitive sandwich enzyme-immunoassay (EIA) using Avidin-Biotin complex was developed for the measurement of GTH II levels in pituitary content and pituitary cell culture medium of the rainbow trout-(Oncorhpchus mykiss). Biotin-salmon GTH II rabbit IgG (sefondary antibody) wai purified by a protein A sepharose affinity chromatography column and that was biotinylated by using Biotin-N-hydroxysuccinimide ofter (BNHS). Non-biotin salmon GTH II rabbit IgG (first antibody) was obtained only through a protein A sepharose affinity chromatography column. The assay was performed by the so-called 'sandwich' method using a microtiter plate, A dose-response curve was obtained between $0.12 to 125 ng/ml$ of salmon GTH II. The displacement curves for pituitary extraction and pituitary cell culture medium of testosterone-treated rainbow trout were Parallel to the standard curie. The intra-assay and inter-assay coefficients of variation (CV) were $8.2{\%} (N=5) and 12.5{\%} (N=6)$, respectively, This assay system was used to measure the amount of GTH II that accumulated in the culture medium of dispersed pituitary cells in testosterone-treated immature rainbow trout, The accumulation was increased with the amount or salmon gonadotropin-releasing hormone. GTH II values determined by the present method were well correlated with those determined by radioimmunoassay. As a result, this assay system was found to be suitable for the measurement of GTH II for pituitary extraction and pituitary culture medium in many salmonid fishes.

• Food Science and Preservation
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• v.9 no.3
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• pp.282-286
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• 2002

This study was conducted to improve the storability of onion bulbs by loading method under room temperature and to reduce the rot caused by field open storage. Allium cepa cv. Changnyungdeago, late strain, was used for the test at the storage condition of 1-row-6-stairs, 2-rows-6-stairs, 4-rows-6-stairs, 1-rows-8-stairs, 2-rows-8-stairs, and 4-rows-8-stairs. The results obtained art as follows: The mean temperature was maintained lowly 1.6∼3.2$\^{C}$ in 1-row-6-stairs and 1.3∼2.6$\^{C}$ in 2-rows-6-stairs in contrast to 4-rows-8-stairs and the relative humidity was high when loading rows increased. The rotting rates in 1-row-6-stairs, 2-rows-6-stairs, 4-rows-6-stairs, 1-rows-8-stairs, 2-rows-8-stairs, and 4-rows-8-stairs were 11.4%, 11.6%, 12.4%, 14.6%, 13.9% and 16.6%, respectively, and became higher with increased rows and stairs of loading. Total weight loss of onion bulbs were l2.2%, 12.7%, 13.8%, 15.5%, 15.2% and 18.0% in 1-row-6-stairs, 2-rows-6-stairs, 4-rows-6-stairs, 1-row-8-stairs, 2-rows-8-stairs, and 4-rows-8-stairs, respectively. The rot of onion bulbs was caused mainly by Fusarium sp., Aspergilus sp., Botrytis sp., and bacteria.

• Journal of Aquaculture
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• v.19 no.4
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• pp.231-235
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• 2006

The effects of mycelium cultural extract supplemented diet, hematology and disease resistance against Vibrio anguillarum in juvenile flounder, Paralichthys olivaceus were evaluated. Fish were fed the Phellinus linteus with Coriolus militaris versicolor mixed mycelium cultural extract supplemented diet, Phellinus linteus mycelium cultural extract supplemented diet and Coriolus militaris tmycelium cultural extract supplemented diet a commercial diet for 12 week. The body weight and length gain from the fish fed on daily the phellinus with coriolus versicolor mixed mycelium cultural extract supplemented diet, phellinus linteus mycelium cultural extract supplemented diet and Coriolus versicolor mycelium cultural extract supplemented diet of each mycelium cultural extract were higher than the control and the Glutamic oxalacetic transaminase (GPT) were of lower than the control. The relative precent survival rate (RPS) after an artificial challenge with $7{\times}10^5\;CFU$ of Vibrio anguillarum per fish was higher than the control.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.1
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• pp.28-33
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• 2008

Traditionally mungbean (Vigna radiata L.) sprouts has been stored after dehydration to decrease their decay. The study was done to determine the effect of watering for final 12 hours of harvest day on morphological characters and color of mungbean sprouts. The seeds of cv. Zhong Lu 1 were soaked in 50 ppm BA solution immediately before 4 hour aeration and then cultured for 6 days, when water-supplied for final 12 hours or not. After packaged with PE envelops, the sprouts stored 5 days at $8^{\circ}C$, and their morphological characters, fresh and dry weights, and colors were measured everyday. Compared to non-water supplied, water supplied sprouts had thicker hypocotyl diameter in middle part, and higher total fresh weight due to increment of cotyledon and hypocotyl ones although the two sprouts did nearly same in other characters. Only nonwater supplied sprouts showed higher color b value in hypocotyl than water supplied ones although there were not significant differences between them in other color-related characters, meaning that the latter ones were more rapidly decayed during their storage. In non-water supplied sprouts, number of lateral roots, hypocotyl and root lengths, total fresh weight, brightness of hypocotyl and root was decreased since 3 days after storage but cutting resistance of hypocotyl was done since 5 days.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.11
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• pp.1584-1592
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• 2014

The objective was to evaluate the ingestive behavior of crossbred heifers finished on a Brachiaria brizantha cv. Marandu pasture receiving four levels of glycerin in their supplementation. Thirty-six crossbred heifers with average initial weight of $264.83{\pm}3.83kg$ and 20 months of age were distributed into a completely randomized design with four treatments and nine replications: control (0%), 4.82%, 10.12%, and 15.56% glycerin in the dry matter. The grazing time reduced linearly (p<0.05), whereas the time spent on activities like rumination, idleness, trough and total chewing time were quadratically affected (p<0.05). Bite rate and number of bites/day were quadratically influenced (p<0.05). The number of bites/swallowed cud and the number of bites/minute, however, increased linearly (p<0.05). Although the time spent on each cud and number of chews per cud were not affected (p>0.05).The number of rumination periods reduced linearly (p<0.05), whereas the number of grazing, idle and trough periods, and the times per grazing, idle, rumination and trough periods were quadratically affected (p<0.05). The feed and rumination efficiencies of the dry matter, non-fibrous carbohydrates, pasture dry matter and concentrate were quadratically affected (p>0.05) whereas the feed efficiency of neutral detergent fiber reduced linearly (p<0.05). Addition of glycerin in substitution of corn in supplements for animals managed on pastures does not influenced feed intake, but reduces the grazing time and increases the idle time. The supplementation also improves feed and rumination efficiencies.

• Journal of Acupuncture Research
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• v.19 no.4
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• pp.208-224
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• 2002

Objective : Gastic cancer has become major cancers which cause nausea, vomiting. Especially patients with terminal stage of gastric cancer may suffer from nausea, vomiting and other symptoms that can keep patients from taking medicine or food. In those cases, there may be no use of taking herbal medicine to treat or palliate symptoms. So we wanted to know the potential efficiency of Acupuncture and Moxibustion whether they could control the symptoms of terminal stage of Gastric cancer without herbal medicine. Methods : Under the assumption that Acupuncture and Moxibustion may be effective for palliating nausea, vomiting on terminal stage of Gastric cancer, the following points were administrated SaGwan(Hapkok($LI_4$), Taechung($LR_3$)), Chok-Samli($ST_{36}$), Kongson($SP_4$), Naegwan($PC_6$) for Acupuncture, Chungwan($CV_{12}$) for Moxibustion. This observation was carried out on 11 patients with terminal stage of Gastric cancer. We reviewed medical records, specifically intake/output check with vomiting, nausea. Results : After therapy of Acupunture and Moxibustion, there were 22% of complete responses, 46% of major responses and 32% of failures. Therapy resulted in 2 cases of goodness, 4 cases of fairness, 5 cases of badness as satisfaction degree. Unfortunately 2 cases of badness expired. Conclusion : We have concluded that Acupunture and Moxibusiton therapy were effective to palliate the nasea, vomiting of terminal Gastric cancer. So if Gastric cancer develop difficulties of taking medicine with patients, to consider using the methods of Acupunture and Moxibution is worthy to palliate the nausea, vomiting and so on.

• Journal of Pharmacopuncture
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• v.7 no.2
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• pp.43-56
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• 2004

Objectives : In order to investigate effects and immune improvement of distilled red-ginseng herbal Acupuncture, expression of Cox-1, Cox-2, and mRNA of Bcl-2 and Bax were analyzed in A549 cell in vivo. Survival time and expression of cytokine mRNA were measured for the mice with Sarcoma-180 induced abdominal cancer. Methods : Balb/c mouse was treated with distilled red-ginseng Herbal Acupuncture at Wisu($BL_{21}$) and Chung- wan($CV_{12}$) to investigate anti-cancer effects and immune response. Results : 1. For expression of mRNA of Cox-1 using RT-PCR, the control group and the experiment groups didn't show significant differences. For Cox-2, both experiment groups and the normal group showed significant differences. 2. For expression of mRNA of Bcl-2 using RT-PCR, experiment groups showed slight decrease compared to the control group. For Bax, no significant changes were shown between the control group and experiment groups. 3. For survival time, all of experiment groups showed 11.1% increase compared to the control group. 4. For IL-2 and IL-4 productivity using Flow cytometry, all of experiment groups didn't show any significance. 5. For IL-2 productivity using ELISA, all of experiment groups didn't show any significance. 6. For expression of cytokine mRNA using RT-PCR, significant increase of IL.-2 and IL-4 were witnessed in the experiment group II compared to the control group. Significant increase of IL-10 was shown in all off experiment groups compared to the control group. Conclusion : According to the results, we can expect that distilled red-ginseng Herbal Acupuncture may be further effccts in anti-cancer and immune improvement if increasing concentration.

• The Korean Journal of Nuclear Medicine Technology
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• v.19 no.2
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• pp.108-111
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• 2015

Purpose Lately, in accordance with the increasing interest about Healthcare accreditation program and International laboratory accreditation scheme, requirements about the instrument quality management are gradually taking shape. In nuclear medicine In vitro laboratory, the most typical instruments are multi detector gamma counter and automatic dispensing system. Each laboratory continue with the quality control adequate for circumstances. The purpose of this study is to application and establish the new Calibrator[I-125]Set which is efficient at standardization of equipment quality management. Materials and Methods Deviation between detectors were measured with 12 solid samples of the Calibrator[I-125]Set. their activities differ from each other by less than 1%. Multi detector gamma counters are GAMMA-10;Shinjin medics. Inc, Goyansi, Korea(Gamma counter A), SR300;Stratec biomedical systems AG, Gewerbestr, Germany(Gamma counter B) and COBRA II; Packard Instrument Co. Inc, Meriden, USA(Gamma counter C). Evaluation of two automatic dispensing system used A, B liquid tracer of the Calibrator[I-125]Set. After dispensing and counting, calculated using the ratio of the measured value and proposed value. We used solution A for 20, 25ul and solution B for 50, 100ul. Method of data analysis and reference range was provided by kit documentation. Furthermore, we could calculate our counter efficiency indirectly. Results The CV(%) of measured values by Gamma counter A, B, C are 0.34, 0.70, 1.30. Calculated value are 1.05314, 2.10419, 4.08485. Provided reference range is less than 3. A dispensing system's calculated values are 0.986, 0.989, 1.023, 1.017 and B are 0.874, 0.725, 1.021, 0.904. Provided reference range is from 0.95 to 1.05. Also, counter's efficiency are 74.18, 72.79, 74.32% at counter A, B, C and efficiency of the one detector counter is 79.26%. Conclusion If using this Calibrator[I-125]Set after verifying whether quality assurance, is applicable to equipment quality management on behalf of the role of gold standard.

• Korean Journal of Environmental Agriculture
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• v.19 no.4
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• pp.324-327
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• 2000

Soybean (Glycine max L. cv. Hwangkeum) seeds were irradiated with the dose of $0{\sim}20$ Gy to investigate the effect of the low dose ${\gamma}-ray$ radiation on the early growth and resistance to subsequent high dose of radiation. Germination rate was not enhanced in the seeds irradiated with low dos ${\gamma}-ray$ but plant height and fresh weight increased in the low dose irradiation group. The optimal radiation dose for the growth increasing was 8 Gy in soybean plant. Growth inhibition of soybean plants by high dose radiation was noticeably reduced by pre-irradiation of low dose radiation, Resistance to subsequent high dose of radiation was effective in 8 Gy and 20 Gy irradiation group.