• Journal of Microbiology and Biotechnology
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• v.21 no.6
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• pp.590-598
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• 2011

Bacterial assimilation of $CO_2$ into stable biomolecules using electrochemical reducing power may be an effective method to reduce atmospheric $CO_2$ without fossil fuel combustion. For the enrichment of the $CO_2$-fixing bacteria using electrochemical reducing power as an energy source, a cylinder-type electrochemical bioreactor with a built-in anode compartment was developed. A graphite felt cathode modified with neutral red (NR-graphite cathode) was used as a solid electron mediator to induce bacterial cells to fix $CO_2$ using electrochemical reducing power. Bacterial $CO_2$ consumption was calculated based on the variation in the ratio of $CO_2$ to $N_2$ in the gas reservoir. $CO_2$ consumed by the bacteria grown in the electrochemical bioreactor (2,000 ml) reached a maximum of approximately 1,500 ml per week. Time-coursed variations in the bacterial community grown with the electrochemical reducing power and $CO_2$ in the mineral-based medium were analyzed via temperature gradient gel electrophoresis (TGGE) of the 16S rDNA variable region. Some of the bacterial community constituents noted at the initial time disappeared completely, but some of them observed as DNA signs at the initial time were clearly enriched in the electrochemical bioreactor during 24 weeks of incubation. Finally, Alcaligenes sp. and Achromobacter sp., which are capable of autotrophically fixing $CO_2$, were enriched to major constituents of the bacterial community in the electrochemical bioreactor.

• Journal of Bio-Environment Control
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• v.13 no.2
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• pp.102-106
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• 2004

Objective of this research was to investigate the effect of fertigation setting point such as -5, -10, -20, and -30 ㎪ on the growth and fruit quality of sweet pepper (Capsicum annuum L.) in greenhouse culture. The net $CO_2$ assimilation and transpiration rate were the lowest in the treatment of -30㎪. The pH and EC in soil solution were not severly affected by irrigation setting point and no statistical differences were observed among treatments of irrigation setting point tested. The N content of above ground plant tissue was the lowest in the treatment of -30 ㎪ and those of K, Ca, and Mg were the highest in the treatment of -10 ㎪. But that of P did not show statistical differences among treatments tested. As the fertigation setting point was getting low, the growth decreased at 60th day after planting, while there were no differences among treatments at 210th day after planting. The fruit quality except sugar contents did not show differences among treatments, but sugar contents was the highest in the treatment of -30 ㎪ with $8.0^{\circ}$Brix. Above results indicated that fertigation setting point should be in the range from -10 ㎪ to -20 ㎪ to ensure good crop growth and fruit quality in sweet pepper production.

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.311-319
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• 2011

Overusing chemical fertilizers involves potassium accumulation in the soil, which can become a toxicity problem in agriculture. This study was conducted to investigate the effect of potassium (K) treatment on growth, physiological characteristics, and morphological changes using Chinese cabbage (Brassica rapa L. ssp. campestris). With high (600 mM) K treatment, the plant growth traits of leaf length, leaf area, and fresh and dry weight of shoots and roots decreased, whereas chlorophyll content increased. As the concentration of K increasing, total N, P, and K increased in leaves, but concentrations of Ca, Mg, and Na decreased. However, Mn, Fe and Zn contents were highest in 100 mM K treatment. Chlorophyll a, b, and carotenoids increased with increasing K concentration. Maximum photochemical efficiency ($F_v/F_m$) was not significant in the all treatments, whereas $CO_2$ assimilation decreased with increasing K level due to stomatal degradation. Total free amino acids increased with the 10 and 100 mM K but decreased at 600 mM K treatments. Therefore, the growth and physiological characteristics of Chinese cabbage ascertained that tolerance up to 100 mM K when grown with nutrient solution in pot culture.

• Horticultural Science & Technology
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• v.32 no.5
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• pp.584-589
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• 2014

Jeffersonia dubia is a spring-flowering perennial found in rich forests in Korea and Northern China and has potential as an ornamental or medicinal plant. However, illegal picking and land use change have decreased the number of populations and overall population size of this plant in its natural habitat. Although J. dubia has been reported to be a shade-preferring plant, no study has determined the optimum light intensity for its growth. The objectives of this work were to observe the effects of various shading levels on the physiological responses of J. dubia and to determine the proper shading level for cultivation. Treatments consisted of four shading levels (0%, 50%, 75%, and 95% shade) imposed using black mesh cloth. The number of leaves and dry weight increased with decreased shading. The shoot-to-root ratio increased with increased shading, mainly due to decreased root dry weight under shading. Plants showed low net $CO_2$ assimilation rates and $F_v/F_m$ values combined with low dry matter levels when grown under 0% shade (full sunlight). These results indicate that J. dubia plants experience excessive irradiance without shading, resulting in damage to the photosynthetic apparatus. By contrast, the net photosynthesis rate increased as the shading level increased. $F_v/F_m$, the potential efficiency of PSII, was 0.8 under 95% shade, indicating that J. dubia is well-adapted under heavy shading. However, the low dry matter of plants in the 95% shade treatment indicated that the low light intensity under 95% shade led to a decline in plant growth. Thus, moderate light (50% shading) is recommended for cultivating J. dubia without physiological defects.

• Journal of Korean Society on Water Environment
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• v.18 no.2
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• pp.189-199
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• 2002

Nitrogen, in its various forms, can deplete dissolved oxygen levels in receiving waters, stimulate aquatic growth, exhibit toxicity toward aquatic life and affect the suitability of sewage for reuse. Pilot-scale Rotating Biological Contactor(RBC) experiments were conducted to examine biological nitrification, respectively, of municipal sewage with five different internal recirculation ratios of 0, 1, 2, 3, and 4 using the constant hydraulic loading of $205L/m^2{\cdot}day$. The use of internal recirculation improved nitrification on account of the dilution of biodegradable organic carbon in influent sewage down to 15 mg/L of $SBOD_5$ or less. Ammonium nitrogen of $14.3{\pm}2.4%$ was consumed by cellular assimilation without the occurrence of denitrification. The thickness of biofilm didn't seem effect significantly the nitrification and denitrification. Nitrification with internal recirculation was found to occur using hydraulic loading rate of as high as $205L/m^2{\cdot}day$, which was beyond the generally known values of it.

• ALGAE
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• v.27 no.4
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• pp.295-301
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• 2012

Carbon concentrating mechanisms play a vital role in photosynthesis in microalgae and cyanobacteria especially in the proper functioning of Rubisco and assimilation of carbon via the Calvin cycle. This study evaluates the role of carbon dioxide on carbon concentrating mechanism (CCM) in a cynaobacteria, Spirulina platensis and a microalga, Chlorella sp. 786. The study organisms were grown in both atmospheric (control sample, 0.035%) and high (exposed sample, 10%) $CO_2$ concentrations. Second dimension (2D) electrophoresis revealed a huge difference in the protein profiles of both organisms suggesting the induction of CCM related proteins in the sample maintained at atmospheric $CO_2$ concentration and the repression of CCM related proteins in the sample maintained at 10% $CO_2$. Liquid chromatography-mass spectroscopy analysis revealed the presence of two important $C_i$ transporter proteins in the control sample of S. platensis, namely ferredoxin-$NADP^+$ reductase and ATP binding cassette (ABC) transport system protein. These proteins were only expressed in the control sample and were downregulated or not expressed at all in the exposed sample. Consequently, this study conclusively proves that CCMs are only inducted at low $CO_2$ concentrations and are not functional at high $CO_2$ concentration.

• Educational Technology International
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• v.6 no.1
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• pp.103-119
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• 2005

This study investigated to designand implement web-based collaborative learning system Co-Net and map out students' learning procedure using the system, based upon Student Team Achievement Division (STAD Slavin, 1990, 1996). There are technical process and instructional considerations to be made during the design process. The former are those that concern equipment requirements and specifications and include Ease of Use, Speed of Access, and Flexibility. On the other hand, instructional considerationsare concerned with the delivery and access of instructional materials and their outcomes on learners. They are cooperative interactions within groups and group heterogeneity, learner control, group incentives, individual accountability, equal opportunity for earning high scores and contributing to group effort, task specialization, and competition among groups. A web site for a virtual learning environment designed and built by the authors and known as Co-Net is then explained along with the whole process learners inside the environment. The main page of Co-Net consists of 15 menus to implement cooperative learning process. The cooperative learning activities using 15 menus are composed of six phases (1) preparation of the new knowledge (2) presentation of the new knowledge (3) knowledge assimilation and application (4) team and individual evaluation (5) team and individual recognition Throughout the five phases, the appropriate use of cooperative learning techniques has been shown to have both academic and social benefits to learners.

• Korean Journal of Agricultural and Forest Meteorology
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• v.22 no.4
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• pp.340-345
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• 2020

In this study, we developed the quality control and assurance method of measurement data of SPAR (Soil-Plant-Atmosphere-Research) system, a climate change research facility, for the first time. It was found that the precise processing of CO2 flux data among many observations were sig nificantly important to increase the accuracy of canopy photosynthesis measurements in the SPAR system. The collected raw CO2 flux data should first be removed error and missing data and then replaced with estimated data according to photosynthetic lig ht response curve model. Comparing the correlation between cumulative net assimilation and soybean biomass, the quality control and assurance of the raw CO2 flux data showed an improved effect on canopy photosynthesis evaluation by increasing the coefficient of determination (R2) and lowering the root mean square error (RMSE). These data processing methods are expected to be usefully applied to the development of crop growth model using SPAR system.

• Applied Biological Chemistry
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• v.11
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• pp.29-33
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• 1969

1. The oxidizability of $NADPH_2$ by quinones in the presence of $NADPH_2$-diaphorase was tested under aerobic conditions. Also the $^{14}CO_2$-fixation rates were compared when Chlorella suspensions were pretreated with $3{\cdot}10^{-5}M$ cocentration of variou quinones for 10 minutes prior and during the $^{14}CO_2$-fixation period. 2. A close correlation seems to exist between the rate of $NADPH_2$ oxidation by quinones and the $^{14}CO_2$-fixation rate. The effect of quinones on $NADPH_2$ oxidation and $^{14}CO_2$-fixation were in the order of Dichlone>06-K>NQ>BQ. 3. It is postulated that the phytotoxicity of quinones on Chlorella is due to the deprival of $NADPH_2$ consequently inhibiting $^{14}CO_2$-fixation, thus causing death of the cells. 4. The effect of quinones on amino acids biosyn-thesis in Chlorella was one of depressed rates, which was especially noted in the case of dichlone. This would be expected from a consideration of $NADPH_2$ deprival and inhibition of $^{14}CO_2$-fixation. Sucrose synthesis was either not affected or rather stimulated, the reasons of which are not clear at the present time.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.3
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• pp.248-251
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• 2003

To find ideal leaf types for soybean breeding program, we examined the relationships among leaf anatomical and morphological characteristics and $\textrm{CO}_2$ assimilation on the different leaflet shape of soybean (Glycine max). In anatomical characters of leaf, palisade and spongy cells were thicker in both small seed cultivars with narrow leaflet and large seed cultivars with wide leaflet than others. $\textrm{CO}_2$ uptake per plant and leaf thickness were significantly associated with seed yield per plant, showing difference among the soybean cultivars. Although the leaf area was lower for narrow leaflet cultivars, which had a significantly higher photosynthetic rate per plant comparable to the wide leaflet cultivars.