• Journal of Nutrition and Health
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• v.39 no.4
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• pp.323-330
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• 2006

Peripheral insulin resistance in obese/type II diabetes animals results from an impairment of insulin-stimulated glucose uptake into skeletal muscle. Insulin stimulate the translocation of GLUT4 from intracellular location to the plasma membrane. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) is implicated in mediation of fusion of GLUT4-containing vesicle with the plasma membrane. Present study investigated regulatory effects of Rhodiola sachalinensis administration and exercise training on the expression of GLUT4 protein and SNAREs protein in skeletal muscles of obese Zucker rats. Experimental animals were randomly assigned into one of five groups ; lean control(LN), obese control(OB), exercise-treated(EXE), Rhodiola sachalinensis-treated(Rho), combine of Rho & EXE (Rho-EXE). All animals of exercise training (EXE, Rho-EXE) performed treadmill running for 8 weeks, and animals of Rho groups (Rho, Rho-EXE) were dosed daily by gastric gavage during the same period. After experiment, blood were taken for analyses of glucose, insulin, and lipids levels. Mitochondrial oxidative enzyme (citrate synthase, CS ; $\beta$-hydroxyacyl-CoA dehydrogenase, $\beta$-HAD) activity were analysed. Skeletal muscles were dissected out for analyses of proteins (GLUT4, VAMP2, syntaxin4, SNAP23). Results are as follows. Exercise and/or Rhodiola sachalinensis administration significantly reduced body weight and improved blood lipids (TG, FFA), and increased insulin sensitivity. Endurance exercise significantly increased the activity of mitochondrial enzymes and the expression of GLUT4 protein, however, administration of Rhodiola sachalinensis did not affect them. The effect of exercise and/or Rhodiola sachalinensis administration on the expression of SNARE proteins was unclear. Our study suggested that improvement insulin sensitivity by exercise and/or Rhodiola sachalinensis administration in obese Zucker rats is independent of expression of SNARE proteins.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.spc
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• pp.47-50
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• 2009

Nutrient losses, especially nitrogen and phosphorus, in agricultural runoff can contaminate surface and ground water, leading to eutrophication. Thus, erosion control is crucial to minimizing nutrient losses from agricultural land. Assessments of various erosion control practices were carried out under various cropping system, soil management practices, and slope conditions by means of a lysimeter study and under artificial rainfall. Soil and nutrient losses were monitored in a small agricultural field to evaluate the soil conservation practices. Nutrient losses occur in runoff and leachate (dissolved nutrient) and in sediments (particulate nutrient). Dissolved nitrates accounted for the majority (about 90%) of nitrate transport within the soil. Particulate phosphate in sediments represented the majority (60% to 67%) of phosphate transport. Recently, engineering and agronomic erosion-control practices haver been used to reduce erosion problems in fields on slopes. These practices reduced soil loss, runoff, and nutrient loss to 1/6, 1/2,and 1/3 their original levels, respectively. Bioavailable particulate phosphate in sediments represents a variable but longterm source of phosphate for algae. Dissolved nitrate and phosphate are immediately available for algal uptake, so reducing fluxes of these nutrients should also reduce the risk of eutrophication.

• Journal of Dairy Science and Biotechnology
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• v.35 no.4
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• pp.249-254
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• 2017

The purpose of this study was to investigate the acid tolerance, bile acid tolerance, and fermentation activity of lactic acid bacteria isolated from Kimchi in the presence of hydrolysates of whey protein concentrate. Kimchi isolates DK109, DK119, DK121, DK128, DK211, DK212, and DK215, which were identified as Lactobacillus sp., and L. casei DK128 showed the highest acid and bile acid tolerance. To produce whey hydrolysates, enzymes were added to a 10% (w/v) whey protein concentrate (WPC) solution at 1:50 (w/v, protein). The viabilities of the DK strains were determined in the presence of low pH and bile salts. Then, yogurt was produced via fermentation with L. casei DK128, an isolate from Kimchi, in the presence of the following additives: CPP, WPC, and WPC hydrolysates (WPCH) generated by alcalase (A) or neutrase (N). The produced yogurts were subjected to various analyses, including viable cell counts (CFU/mL), pH, titratable activity, and sensory testing. After 8 h of fermentation, the pH and titratable activity values of all test samples were 4.2 and 0.9, respectively. The viable counts of LAB were $3.49{\times}10^8$, $5.72{\times}10^8$, $7.01{\times}10^8$, and $6.97{\times}10^8$, for the Control, CPP, A, and N samples, respectively. These results suggest that whey proteins have potential as dietary supplements in functional foods and that WPCH could be used in yogurt as a low-cost alternative to CPP.

• Toxicological Research
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• v.28 no.4
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• pp.235-240
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• 2012

$^{99m}Tc$ tricarbonyl glycine monomers, trimers, and pentamers were synthesized and evaluated for their radiolabeling and in vivo distribution characteristics. We synthesized a $^{99m}Tc$-tricarbonyl precursor with a low oxidation state (I). $^{99m}Tc(CO)_3(H_2O)_3^+$ was then made to react with monomeric and oligomeric glycine for the development of bifunctional chelating sequences for biomolecules. Labeling yields of $^{99m}Tc$-tricarbonyl glycine monomers and oligomers were checked by high-performance liquid chromatography. The labeling yields of $^{99m}Tc$-tricarbonyl glycine and glycine oligomers were more than 95%. We evaluated the characteristics of $^{99m}Tc$-tricarbonyl glycine oligomers by carrying out a lipophilicity test and an imaging study. The octanol-water partition coefficient of $^{99m}Tc$ tricarbonyl glycine oligomers indicated hydrophilic properties. Single-photon emission computed tomography imaging of $^{99m}Tc$-tricarbonyl glycine oligomers showed rapid renal excretion through the kidneys with a low uptake in the liver, especially of $^{99m}Tc$ tricarbonyl triglycine. Furthermore, we verified that the addition of triglycine to prototype biomolecules (AGRGDS and RRPYIL) results in the improvement of radiolabeling yield. From these results, we conclude that triglycine has good characteristics for use as a bifunctional chelating sequence for a $^{99m}Tc$-tricarbonyl-based biomolecular imaging probe.

• ALGAE
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• v.38 no.1
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• pp.81-92
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• 2023

Obesity-induced inflammation is crucial in the pathogenesis of insulin resistance and type 2 diabetes. In this study, we investigated the effects of the Gracilaria chorda (GC) on lipid accumulation and obesity-induced inflammatory changes or glucose homeostasis in cell models (3T3-L1 adipocytes and RAW 264.7 macrophages). Samples of GC were extracted using solvents (water, methanol, and ethanol) and subcritical water (SW) at different temperatures (90, 150, and 210℃). The total phenolic content of GCSW extract at 210℃ (GCSW210) showed the highest content compared to others, and GCSW210 highly inhibited lipid accumulation and significantly reduced gene expressions of peroxisome proliferator-activated receptor-γ, CCAAT/enhancer-binding protein-α, sterol regulatory element-binding protein-1c, and fatty acid synthase in 3T3-L1 adipocytes. In addition, GCSW210 effectively downregulated the pro-inflammatory cytokine regulator pathways in RAW 264.7 macrophages, including mitogen-activated protein kinase, signal transducers and activators of transcription and nuclear factor-κB. In co-culture of 3T3-L1 adipocytes and RAW 264.7 macrophages, GCSW210 significantly reduced nitric oxide production and interleukin-6 levels, and improved glucose uptake with dose-dependent manner. These findings suggest that GCSW210 improves glucose metabolism by attenuating obesity-induced inflammation in adipocytes, which may be used as a possible treatment option for managing obesity and associated metabolic disorders.

• BMB Reports
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• v.57 no.2
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• pp.92-97
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• 2024

Elevated blood glucose is associated with an increased risk of atherosclerosis. Data from the current study showed that glucosamine (GlcN), a normal glucose metabolite of the hexosamine biosynthetic pathway (HBP), promoted lipid accumulation in RAW264.7 macrophage cells. Oleic acid- and lipopolysaccharide (LPS)-induced lipid accumulation was further enhanced by GlcN in RAW264.7 cells, although there was no a significant change in the rate of fatty acid uptake. GlcN increased acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), scavenger receptor class A, liver X receptor, and sterol regulatory element-binding protein-1c (SREBP-1c) mRNA expression, and; conversely, suppressed ATP-binding cassette transporter A1 (ABCA-1) and ABCG-1 expression. Additionally, GlcN promoted O-GlcNAcylation of nuclear SREBP-1 but did not affect its DNA binding activity. GlcN stimulated phosphorylation of mammalian target of rapamycin (mTOR) and S6 kinase. Rapamycin, a mTOR-specific inhibitor, suppressed GlcN-induced lipid accumulation in RAW264.7 cells. The GlcN-mediated increase in ACC and FAS mRNA was suppressed, while the decrease in ABCA-1 and ABCG-1 by GlcN was not significantly altered by rapamycin. Together, our results highlight the importance of the mTOR signaling pathway in GlcN-induced macrophage lipid accumulation and further support a potential link between mTOR and HBP signaling in lipogenesis.

• Journal of Electrochemical Science and Technology
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• v.15 no.3
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• pp.365-372
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• 2024

As the energy density of lithium-ion batteries (LIBs) continues to increase, various separators are being developed to with the aim of improving the safety performance. Although poly(imide) (PI)-based separators are widely used, it is difficult to control their pore size and distribution, and this may further increase the risk associated. Herein, a melamine phosphonic acid (MP)-coated PI separator that can effectively control the pore structure of the substrate is suggested as a remedy. After the MP material is embedded into the PI separator with a simple one-step casting process, it effectively clogs the large pores of the PI separator, preventing the occurrence of internal short circuits during charging. It is anticipated that the MP material can also suppress rapid thermal runaway upon cycling due to its ability to reduce the internal temperature of the LIB cell caused by the desirable endothermic behavior around 300℃. According to experiments, the MP-coated PI separator not only decreases the thermal shrinkage rate better than commercial poly(ethylene) (PE) separators but also exhibits a desirable Gurley number (109.6 s/100 cc) and electrolyte uptake rate (240%), which is unique. The proposed separator is electrochemically stable in the range 0.0-5.0 V (vs. Li/Li⁺), which is the typical working potential of conventional electrode materials. In practice, the MP-coated PI separator exhibits stable cycling performance in a graphite-LiNi_0.83Co_0.10Mn_0.07O₂ full cell without an internal short circuit (retention: 90.3%).

• Korean Journal of Food Science and Technology
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• v.39 no.3
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• pp.330-335
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• 2007

This study was performed to investigate the anti-diabetic mechanism of crude polysaccharides isolated from the fruiting bodies of Grifola frondosa. We treated 3T3-L1 adipocyte cells to observe whether the crude polysaccharides isolated from Grifola frondosa would stimulate insulin sensitivity. Significant insulin sensitizing activity was observed in the 3T3-L1 adipocytes, and giving the crude polysaccharide of Grifola frondosa with 1 nM of insulin caused glucose uptake to increase to a similar level as giving 50 nM of insulin alone. To confirm the mechanism for the anti-diabetic effect of the crude polysaccharides, we performed further examinations within $KK-A^{y}$ mice, an animal model of type 2 diabetes. The crude polysaccharides reduced blood glucose levels in the $KK-A^{y}$ mice for 2 weeks after feeding, and also significantly lowered plasma insulin levels. These results suggest that the anti-diabetic mechanism of the crude polysaccharide of Grifola frondosa is related to the enhancement of insulin sensitivity.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.229-233
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• 2010

This experiment was carried out to find optimum ratio of $NO_3^-$ to $NH_4^+$ in nutrient solution for the growth of Phalaenopsis hybrid and find the effect of the ratio of $NO_3^-$ to $NH_4^+$ on the flower stem (inflorescence) quality. The ratio of $NO_3^-$ to $NH_4^+$ of nutrient solution used in this experiment was 100% : 0%, 90% : 10%, 80% : 20% and 70% : 30%. Phalaenopsis showed better growth when $NH_4^+$ was supplied concurrently with $NO_3^-$ as nitrogen source than supplied with only $NO_3^-$. Especially, increasing the ratio of $NH_4^+$ from 0% to 10% the fresh weight and dry weight of Phalaenopsis hybrid was highest. But, the growth of shoot and root was diminished when the proportion of $NH_4^+$ in nutrient solution was increased from 10% to 30%. Inflorescence length, the number of inflorescence and flower per plant all increased as $NH_4^+$ increased from 0% to 10% but, decreased from 10% to 30%. These results suggest that the optimal ratio of $NO_3^-$ and $NH_4^+$ in nutrient solution for the growth of Phalaenopsis including inflorescence was founded to be 90%:10%.

• Journal of the Korean Recycled Construction Resources Institute
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• v.11 no.4
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• pp.324-331
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• 2023

In this study, the hydration and carbonation properties of circulating fluidized bed boiler (CFBC) ash with different free-CaO contents were investigated. In addition, the possibility of utilizing CFBC ash with a high free-CaO content as a cementitious material was investigated by carbonation curing as a pretreatment. The CFBC ash with high free-CaO content exhibited rapid setting behavior and low early compressive strength when mixed with cement. For CFBC ash with high free-CaO content, carbon dioxide capture increased with the duration of carbonization curing. In addition, the free-CaO value decreased together, indicating that the free-CaO reacted with carbon dioxide. When the CFBC ash with high free-CaO content was pretreated by carbonation, no fresh set appeared, and the initial compressive strength was improved. From the results of this study, it is confirmed that CFBC ash with high free-CaO content has a high potential to be utilized as a cementitious material through proper carbonation curing.