• Journal of Nutrition and Health
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• v.37 no.5
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• pp.347-351
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• 2004

This study was carried out to investigate the supplementary effects of ${\gamma}$-oryzanol extracted from rice bran on lipid metabolism in diabetic mice. We supplied 2 kinds of experimental diets (CO without and GO with 0.2％ ${\gamma}$-oryzanol) to diabetic mice for 8 weeks. Diet intake, body weight, organ weight, contents of serum and hepatic lipid profiles, and fecal lipid levels were measured. Though there was no significant difference in diet intake, body weight change and organ weight between experimental groups, the concentration of serum total cholesterol and hepatic total lipid, total cholesterol and HMG-CoA reductase activity was significantly lower in GO group treated with 0.2％ ${\gamma}$-oryzanol of diet than CO group after supplementary period of experimental diets. And total lipid, triglyceride, total cholesterol, and bile acid levels excreted to feces were significantly higher in GO group than CO group. These results suggest that ${\gamma}$-oryzanol decrease the serum and hepatic lipid levels by lowing HMG-CoA reductase activity or increasing the contents of fecal lipid in diabetic KK mice.

• Ocean and Polar Research
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• v.37 no.3
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• pp.201-209
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• 2015

To examine the effects of increased $CO_2$ concentration and seawater temperature on the photosynthesis and growth of forest forming Ecklonia cava (Laminariales, Phaeophyta), sporophytic discs and gametophytes were cultured under three $pCO_2$ concentrations (380, 750, 1000 ppm), four temperatures (5, 10, 15, $20^{\circ}C$ for sporophytes; 10, 15, 20, $25^{\circ}C$ for gametophytes), and two irradiance levels (40, $80{\mu}mol$ photons $m^{-2}s^{-1}$) for 5 days. Photosynthetic parameter values ($ETR_{max}$, $E_k$, and ${\alpha}$) were generally higher as sporophytic discs were grown under low temperature and increased $CO_2$ concentration at 750 ppm. However, photosynthesis of Ecklonia sporophytes was severely inhibited under a combination of high temperature ($20^{\circ}C$) and 1000 ppm $CO_2$ concentration at the two photon irradiance levels. The growth of gametophytes was maximal at the combination of 380 ppm (present seawater $CO_2$ concentration) and $25^{\circ}C$. Minimal growth of gametophytes occurred at enriched $pCO_2$ concentration levels (750, 1000 ppm) and high temperature of $25^{\circ}C$. The present results imply that climate change which is increasing seawater temperature and $pCO_2$ concentration might diminish Ecklonia cava kelp beds because of a reduction in recruitments caused by the growth inhibition of gametophytes at high $pCO_2$ concentration. In addition, the effects of increased temperature and $pCO_2$ concentration were different between generations - revealing an enhancement in the photosynthesis of sporophytes and a reduction in the growth of gametophytes.

• Korean Journal of Human Ecology
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• v.20 no.5
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• pp.1075-1091
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• 2011

The purposes of this study were to investigate the actual state of indoor air quality in semi-underground multi-family housing units in early summer, to analyze the influencing factors, and to make suggestions for improvement. A series of field investigations were conducted in four target units between June 22, 2010, and June 28, 2010. The field investigations included measurements of indoor air quality as well as the observation of architectural characteristics and living conditions. In addition to the field investigation, on-site questionnaire surveys were administrated to residents in 90 units. The findings are summarized as follows: (1) The average $CO_2$ levels in each of the four units ranged from 759ppm to 1885ppm. $CO_2$ levels in three units exceeded the evaluation standard (1000ppm), and one unit was lower than the standard. This unit had smaller number of staying person and a large amount of ventilation than others; (2) the average CO levels in each unit were almost 0ppm, but 0.1~1.1 levels of CO were revealed at several times; (3) the average PM-10 levels ranged from $8{\mu}g/m^3$ to $40{\mu}g/m^3$, which distributes within the standard ($150{\mu}g/m^3$). The influencing factors of PM-10 levels were analyzed as inflow from outside, the use of a gas range, and the residents' activities; (4) therefore, it was evaluated to be necessary to improve ventilation for the indoor air quality of semi-underground housing in aspect of $CO_2$ levels.

• Fisheries and Aquatic Sciences
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• v.12 no.4
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• pp.350-353
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• 2009

As a result of human industrial development, carbon dioxide ($CO_2$) is currently accumulating in the atmosphere and dissolving into the oceans. Sequestration into the deep sea has been proposed as a possible solution to this increasing atmospheric $CO_2$, although the impact of such a program on marine ecosystems is unknown. We examined the effects of increased $CO_2$ levels on the growth of the olive flounder, Paralichthys olivaceus. Juvenile olive flounder 40 days post hatching were exposed to two levels of $CO_2$ (3.60-7.55 and 4.05-11.46 kPa) in running seawater for 26 days. During the exposure period, the pH and $CO_2$ levels of the water were measured, and the numbers of dead individuals were counted in each aquarium. Following the exposure period, the total lengths (mm) and body weights (mg) of the juvenile fish were measured. Both $CO_2$ treatments significantly increased fish mortality compared to controls ($19.87\pm4.53%$ vs. 7.14% and $75.96\pm1.36%$ vs. 7.14% for high and low doses, respectively). After the high $CO_2$ treatment, total length ($14.98\pm6.58$ mm vs. $19.52\pm1.83$ mm) and body weight ($28.92\pm13.85$ mg vs. $67.35\pm18.32$ mg) of the exposed flounder were reduced compared to the control fish; however, no significant differences in these values were observed after the low $CO_2$ dose. These results suggested that $CO_2$ exposure inhibits growth in the juvenile stage and that $CO_2$-enriched seawater is toxic in the early life stages of olive flounder.

• Journal of Environmental Health Sciences
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• v.32 no.2 s.89
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• pp.132-139
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• 2006

Number of aerosol, CO, $CO_2$ and TVOC after one-, two-, three-cigarettes smoking were monitored with time every 10 minute for 180 minutes in the seminar room (volume $51.1m^3$) when poorly-ventilated. IAQ monitor (IAQRAE, model PGM-5210) and PortCount (TSI, model 8020) were used for monitoring. Aerosol was decreased with exponential decay equation and it was estimated that number of aerosol would be long suspended (one cigarette 75/cc. two cigarettes 66/cc, three cigarettes 141/cc by 8hrs after smoking). While CO was also decreased with exponential or linear decay equation and correlated with number of aerosol strongly, TVOC and $CO_2$ were increased with linear equation in accordance with time lag. Most of TVOC and $CO_2$ were above standard levels of Korean Indoor Air Quality (Ministry of Environment) without regarding number of cigarettes. When naturally ventilated, all of CO, $CO_2$ and TVOC concentrations were dramatically decreased below standard levels of Korean Indoor Air Quality.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.303-309
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• 2006

The study examined the effects of $CO_2$ enrichment on growth of soybean (Glycine max). Two soybean varieties were used, Taekwang and Cheongja. The plants were grown in growth chambers with a 12-h photoperiod and a day/night temperature of $28/21^{\circ}C$ at the seedling stage and $30/23^{\circ}C$ from the flowering stage. The plants were exposed to the two elevated $CO_2$ levels of 500 and 700 ppm and the ambient level of 350 ppm. Results of the experiment showed that at the second-node trifoliate stage of the two varieties, the elevated $CO_2$ increased plant height, leaf area and dry weight. The elevated $CO_2$ also raised the photosynthetic rate of soybean as compared to the ambient level. From the beginning bloom stage to the full maturity stage of the two varieties, the elevated $CO_2$ increased plant height, leaf area, seed weight and photosynthetic rate. The stomatal conductance and transpiration rate decreased on long days relative to short days of treatment. Through the entire stages, the elevated $CO_2$ increased the water use efficiency of soybean plants because stomatal conductance and transpiration rate decreased at the elevated $CO_2$ levels relative to the ambient level.

• The Plant Pathology Journal
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• v.33 no.5
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• pp.522-527
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• 2017

We determined the effects of atmospheric temperature ($10-30{\pm}2^{\circ}C$ in $5^{\circ}C$ increments) and carbon dioxide ($CO_2$) levels ($400{\pm}50ppm$, $540{\pm}50ppm$, and $940{\pm}50ppm$) on the infection of Solanum tuberosum cv. Chubaek by Potato leafroll virus (PLRV). Below $CO_2$ levels of $400{\pm}50ppm$, the PLRV infection rate and RNA content in plant tissues increased as the temperature increased to $20{\pm}2^{\circ}C$, but declined at higher temperatures. At high $CO_2$ levels ($940{\pm}50ppm$), more plants were infected by PLRV at $30{\pm}2^{\circ}C$ than at 20 or $25{\pm}2^{\circ}C$, whereas PLRV RNA content was unchanged in the $20-30{\pm}2^{\circ}C$ temperature range. The effects of atmospheric $CO_2$ concentration on the acquisition of PLRV by Myzus persicae and accumulation of PLRV RNA in plant tissues were investigated using a growth chamber at $20{\pm}2^{\circ}C$. The M. persicae PLRV RNA content slightly increased at elevated $CO_2$ levels ($940{\pm}50ppm$), but this increase was not statistically significant. Transmission rates of PLRV by Physalis floridana increased as $CO_2$ concentration increased. More PLRV RNA accumulated in potato plants maintained at 540 or $940{\pm}50ppm$ $CO_2$, than in plants maintained at $400{\pm}50ppm$. This is the first evidence of greater PLRV RNA accumulation and larger numbers of S. tuberosum plants infected by PLRV under conditions of combined high $CO_2$ levels ($940{\pm}50ppm$) and high temperature ($30{\pm}2^{\circ}C$).

• Journal of Korean Neurosurgical Society
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• v.55 no.5
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• pp.237-243
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• 2014

Objective : Symptomatic disc degeneration develops from inflammatory reactions in the annulus fibrosus (AF). Although inflammatory mediators during annular inflammation have been studied, the roles of matrix metalloproteinases (MMPs) and their inhibitors have not been fully elucidated. In this study, we evaluated the production of MMPs and tissue inhibitors of metalloproteinase (TIMPs) during annular inflammation using an in vitro co-culture system. We also examined the effect of notochordal cells on annular inflammation. Methods : Human AF (hAF) pellet was co-cultured for 48 hours with phorbol myristate acetate-stimulated macrophage-like THP-1 cells. hAF pellet and conditioned media (CM) from co-cultured cells were assayed for MMPs, TIMPs, and insulin-like growth factor (IGF)-1 levels using real-time reverse-transcriptase polymerase chain reaction and enzyem-linked immunosorbent assay. To evaluate whether notochordal cells affected MMPs or TIMPs production on annular inflammation, hAF co-cultured with notochordal cells from adult New Zealand White rabbits, were assayed. Results : MMP-1, -3, -9; and TIMP-1 levels were significantly increased in CM of hAF co-cultured with macrophage-like cells compared with hAF alone, whereas TIMP-2 and IGF-1 levels were significantly decreased (p<0.05). After macrophage exposure, hAF produced significantly more MMP-1 and -3 and less TIMP-1 and -2. Interleukin-$1{\beta}$ stimulation enhanced MMP-1 and -3 levels, and significantly diminished TIMP-2 levels. Co-culturing with rabbit notochordal cells did not significantly influence MMPs and TIMPs production or COL1A2 gene expression. Conclusion : Our results indicate that macrophage-like cells evoke annular degeneration through the regulation of major degradative enzymes and their inhibitors, produced by hAF, suggesting that the selective regulation of these enzymes provides future targets for symptomatic disc degeneration therapy.

• Journal of Korean Society for Atmospheric Environment
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• v.3 no.2
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• pp.27-32
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• 1987

As part of an air pollution epidemiological study of asthmatics residing in the Houston area, an air monitoring system provided data on the indoor and outdoor measurements of major pollutant gases sampled at selected residences during May ~ October 1981. Continuously monitored pollutant gases included sulfur dioxide ($SO_2$), nitrogen dioxide($NO_2$), nitric oxide(NO), carbon monoxide(CO), and ozone($O_3$). Outdoor levels for each pollutant were compared with their indoor levels(bedroom, kitchen, living room). Mean concentrations of each pollutant in the kitchen, and living room exceeded the mean levels outside except for ozone, while average bedroom levels for all gases except for $O_3$ and $NO_2$ were found higher than the corresponding outside levels. Indoor/ outdoor ratios for $SO_2$, NO, and CO were 1.8 ~ 2.7 times the outdoor levels, but indoor/ outdoor ratios for $NO_2$ and $O_3$ were 0.99 and 0.06, respectively. The impact of several important household characteristics (type of cooking fuel and cigarette smoking) on the indoor levels for these gases is evaluated.

• Journal of Microbiology and Biotechnology
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• v.17 no.3
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• pp.414-419
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• 2007

Botryococcus braunii is a green colonial fresh water microalga and it is recognized as one of the renewable resources for production of liquid hydrocarbons. CFTRI-Bb-l and CFTRI-Bb-2 have been reported for the first time and their performance with regard to growth and biochemical profile is presented here. The present study focused on effect of carbon dioxide $(CO_2)$ on biomass, hydrocarbon, carbohydrate production, fatty acid profile, and carotenoid content in various species of B. braunii (LB-572, SAG 30.81, MCRC-Bb, N-836, CFTRI-Bb-l, and CFTRI-Bb-2) at 0.5, 1.0, and 2.0% (v/v) levels using a two-tier flask. $CO_2$ at 2.0% (v/v) level enhanced growth of the organism, and a two-fold increase in biomass and carotenoid contents was observed in all the B. braunii strains studied compared with control culture (without $CO_2$ supplementation). At 1 % and 2% (v/v) $CO_2$ concentrations, palmitic acid and oleic acid levels increased by 2.5 to 3 folds in one of the strains of B. braunii (LB-572). Hydrocarbon content was found to be above 20% at 2% $CO_2$ level in the B. braunii LB-572, CFTRI-Bb-2, CFTRI-Bb-l, and N-836 strains, whereas it was less than 20% in the SAG 30.81 and MCRC-Bb strains compared with control culture. This culture methodology will provide information on $CO_2$ requirement for growth of algae and metabolite production. B. braunii spp. can be grown at the tested levels of $CO_2$ concentration without much influence on culture pH.