• Journal of Plant Biology
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• v.36 no.4
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• pp.383-389
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• 1993

The effects of light and $CO_2$ on the electrophysiological characteristics of guard cells in the intact leaf and in the detached epidermis have been investigated. Guard cells in intact leaves showed the membrane hyperpolarization in response to light. The biggest induced change of the membrane potential difference (PD) in the guard cells of the intact leaf was 13 m V by light and 42 mV by $CO_2$ in Commelina communis. Similar results were obtained with Tradescantia virginiana. However, there were no changes of membrane PD in detached epidermis. In order to determine the influence of the mesophyll on the changes of membrane PD, infiltration of the mesophyll cells with photosynthetic inhibitors was performed. In CCCP infiltrated leaf discs the guard cell membrane was depolarized slightly by red-light and hyperpolarized by $CO_2$, but in leaf discs infiltrated with DCCD and DCMU the guard cell membrane was hyperpolrized by both red-light and $CO_2$ as the control leaf discs. In azide infiltrated leaf discs the guard cell membrane showed no response to light and there was a much reduced membrane hyperpolarization by $CO_2$ compared to other responses. It was likely that azide caused leaf damage and the activity of cell metabolism was decreased greatly, resulting in small membrane PD changes by $CO_2$ and no changes by redlight. Therefore, it can be suggested that red light was sensed by the mesophyll and the light induced guard cell membrane hyperpolarization was related to energy produced by cyclic-photophosphorylation, but ${CO_2}-induced$ guard cell membrane hyperpolarization was not related to photosynthesis. Alkalisation of the vacuole was observed when the intact leaf was exposed to $CO_2$, indicating that membrane hyperpolarization was mainly the result of proton efflux.efflux.

• Ecology and Resilient Infrastructure
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• v.8 no.2
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• pp.133-133
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• 2021

• Journal of Pharmaceutical Investigation
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• v.31 no.4
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• pp.209-216
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• 2001

The purpose of the present study was to investigate the hepatic uptake and biliary excretion of l-anilino-8-naphthalene sulfonate (ANS) in vivo. The plasma concentration and liver concentration of ANS were determined after its i.v. bolus administration at a dose of $30\;{\mu}mol/kg$ in rats. The hepatic uptake clearance $(CL_{uptake})$ of ANS was 0.1 ml/min/g liver. On the basis of the unbound concentration of ANS, the permeability-surface area product $(PS_{influx})$ was calculated to be l0.4 ml/min/g liver, being comparable of in vitro data. On the other hand, we determined the plasma concentration, liver concentration and biliary excretion rate of ANS at steady-state after its i. v. infusion $(0.2-1.6\;{\mu}mol/min/kg)$ in rats. The excretion clearance $(CL_{excretion})$ of ANS showed Michaelis-Menten kinetics with increasing the infusion rate. The permeability-surface area product $(PS_{excretion})$ based on the unbound concentration in the liver was calculated to be 0.0165 ml/min/g liver, which is negligible compared with the intrinsic clearance $(CL_{int}=3.3\;ml/min/g\;liver)$ by rat liver microsomes. The sequestration process of ANS, therefore, was considered to be mainly due to the metabolic process in the liver $(PS_{seq}{\risingdotseq}CL_{int})$. Furthermore, $PS_{efflux}$ value calculated from $PS_{influx}$ and $PS_{seq}$ was 4.4 ml/min/g liver, which was comparable of in vitro data. In conclusion, in vivo parameters such as $PS_{influx}$, $PS_{efflux}$ and $PS_{seq}$ in the present study showed good in vivo-in vitro relationship. Thus, the kinetic analysis method proposed in the present study would be useful to analyze the hepatic transport of drugs in vivo.

• Biomolecules & Therapeutics
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• v.25 no.5
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• pp.553-558
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• 2017

Paclitaxel (PTX) is one of the most frequently used anticancer agent for treating refractory ovarian cancer, metastatic breast cancer and non-small cell lung cancer. However, its oral administration is impeded by very low bioavailability (<5%) due to the P-glycopprotein (P-gp) efflux pump effect. This study investigated in vitro and in vivo P-gp inhibitory effects of adamantyl derivatives AC-603 and AC-786 in rats. Two adamantyl derivatives tested in this study increased the cytotoxicity of daunomycin (DNM) in P-gp overexpressed cell line by inhibiting P-gp efflux function. Pharmacokinetics of PTX with orally co-administered P-gp inhibitors were assessed in rats to improve PTX absorption. The pharmacokinetic parameters of PTX were determined in rats after intravenous (2 mg/kg) or oral (25 mg/kg) administration in the presence or absence of verapamil (a positive control), AC-603 or AC-786 (0.5 mg/kg or 5 mg/kg). Compared to control group (PTX alone), experimental groups (PTX with AC-603 or AC-786) significantly increased the area under the plasma concentration-time curve of PTX following oral administration by 1.7-2.2 fold. The volume of distribution and total clearance of PTX were decreased, while other parameters were not significantly changed. In conclusion, co-administration of AC-603 or AC-786 enhanced the relative bioavailability of orally administered PTX as compared to control.

• Toxicological Research
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• v.31 no.2
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• pp.137-149
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• 2015

Human hepatocytes, with complete hepatic metabolizing enzymes, transporters and cofactors, represent the gold standard for in vitro evaluation of drug metabolism, drug-drug interactions, and hepatotoxicity. Successful cryopreservation of human hepatocytes enables this experimental system to be used routinely. The use of human hepatocytes to evaluate two major adverse drug properties: drug-drug interactions and hepatotoxicity, are summarized in this review. The application of human hepatocytes in metabolism-based drug-drug interaction includes metabolite profiling, pathway identification, P450 inhibition, P450 induction, and uptake and efflux transporter inhibition. The application of human hepatocytes in toxicity evaluation includes in vitro hepatotoxicity and metabolism-based drug toxicity determination. A novel system, the Integrated Discrete Multiple Organ Co-culture (IdMOC) which allows the evaluation of nonhepatic toxicity in the presence of hepatic metabolism, is described.

• Animal cells and systems
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• v.10 no.4
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• pp.191-196
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• 2006

This study was carried out to evaluate soil carbon cycling of a 36-year-old larch (Larix leptolepis) stand in Korea. The aboveground and soil organic carbon storage, litterfall, and soil respiration rates were measured over twoyear periods. The estimated aboveground biomass carbon storage and increment were 4220 gC $m^{-2}$ and 150 gC $m^{-2}\;yr^{-1}$, respectively. Mean organic carbon inputs by needle and total litterfall were 118 gC $m^{-2}\;yr^{-1}$ and 168 gC $m^{-2}\;yr^{-1}$, respectively. The aboveground carbon increment of the stand was similar to the annual input of carbon from total litterfall. The soil respiration rates correlated exponentially with the soil temperature at a depth of 20 cm ($R^2$ = 0.86). In addition, the exponential regression equation indicated a relatively strong positive relationship between the soil respiration rates and soil temperature, while there was no significant relationship between the soil respiration rates and the soil moisture content. The annual mean and total soil respiration rates were 0.40 g $CO_2\;m^{-2} h^{-1}$ and 3010 g $CO_2\;m^{-2}\;yr^{-1}$ over the two-year study period, respectively.

• Journal of Ecology and Environment
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• v.35 no.4
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• pp.307-311
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• 2012

This study was conducted to evaluate the dynamics of soil respiration (total soil and heterotrophic respiration) following fertilizer application in red pine forests. Fertilizer (N:P:K = 113:150:37 kg/ha), which reflects current practices in Korean forest, was applied in April 2011, and total soil and heterotrophic respiration rates were monitored from April 2011 to March 2012. Monthly variation of total soil and heterotrophic respiration rates were similar between the fertilizer and control treatments, as soil temperature was the dominant factor controlling the both rates. Total soil respiration rates during the study period were not significantly different between the fertilizer (0.504 g $CO_2\;m^{-2}\;h^{-1}$) and control (0.501 g $CO_2\;m^{-2}\;h^{-1}$) treatments. However, the proportion of heterotrophic respiration was higher in the fertilizer (78% of total soil respiration rates) than in the control (62% of total soil respiration rates) treatments. These results suggest that current fertilizer practices in Korea forest soil do not substantially affect total soil respiration rates.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.1179-1186
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• 2012

For better understanding of carbon cycle dynamics of an agro-ecosystem, an accurate assessment of seasonal and daily $CO_2$ flux is essential to understand the relationship between various environmental factors and crop productivity. We developed the automatic sliding canopy chamber (ASCC) system that measured continuous net ecosystem productivity (NEP) over whole growing season under the natural meteorological rhythm. The ASCC was composed of two main parts which were sliding part for measuring NEP, and automatic opening and closing chamber (AOCC) for measuring soil respiration (SR) on the soil surface. The ASCC was developed by using open flow method for measuring soil $CO_2$ efflux. The disturbance of natural meteorological condition was minimized by opening the base frames. In the field test with barley (Hordeum vulgare L.), NEP was calculated at $140mg\;CO_2\;m^{-2}h^{-1}$ on a clear day using continuous data and eliminated the possibility of overestimate about 16% using one hour data during the day time. Unlike other small scale chamber system, installation on cropping-field made it possible to take any modifications which might be caused by natural environmental condition.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.4
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• pp.350-359
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• 1993

The effect of gibberellic acid ($GA_3) and abscisic acid (ABA) on KCl-enhanced proteolysis of senescing leaves of rice(Oryza sativa L. cv. Chilsung) was studied. Emphasis was given to their effects on KCI-enhanced efflux of amino acids and proteinase activity. When treated singly, $GA_3 affected leaf proteolysis little, while ABA increased proteolysis, the rate of amino acid efflux, and ribulose -1,5 -bisphosphate carboxylase / oxygenase (Rubisco)-degrading endoproteinase activity. An additive increase in all three parameters mentioned above was observed when leaves were treated with ABA and KCl. No such an additive effect was found when $GA_3 was treated with KCl. Both $GA_3 and ABA helped to alleviate the KCI-suppressed activity of Rubisco-degrading exoproteinases. The additive increase in proteolysis of rice leaves in the presence of both ABA and KCl could thus be ascribed to a further increase in the efflux of protein hydrolyzates and Rubisco-degrading endoproteinase activity. An increase in proteolysis was accompanied by a decrease in water absorption, and the combined treatment of ABA with KCl resulted in a further reduction of water absorption.

• Journal of Korean Society of Forest Science
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• v.95 no.2
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• pp.168-173
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• 2006

Stem respiration rate ($R_{stem}$) was examined using an open flow system on black locust (Robinia pseudoacacia L.). $R_{stem}$ exponentially increased with increasing air and stem temperature during measurement period and was most closely correlated with stem temperature. It was more closely correlated with stem temperature observed 0.5-2.0 hrs earlier than with current stem temperature, that is, there was time lag between the increase of stem temperature and the efflux of $CO_2$ from stem. $R_{stem}$ gradually increased from spring to summer, and then decreased during autumn. $R_{stem}$ ranged from 0.13 to $4.44{\mu}mol$ $CO_2m^{-2}s^{-1}$. $Q_{10}$ decreased with increasing stem temperature, and varied along position (or diameter) within trees during the same period, especially during the growing season. On the other hand, time lag was shortened with increasing temperature.