• 한국신재생에너지학회:학술대회논문집
• /
• 2009.06a
• /
• pp.821-824
• /
• 2009

LFG-MGT CHP system development project with $CO_2$ enrichment in greenhouses was introduced. LFG is produced from the anaerobic digestion of landfilled waste and it has been utilized for power/heat generation since it contains around 50% of $CH_4$. Utilization of LFG from small scale landfill is also needed as well as large scale landfill. However, due to economy of scale, it is very difficult to develop business model. In this context, combining CHP system with greenhouses is considered as feasible option for LFG utilization. LFG-MGT CHP system with $CO_2$ fixation in greenhouses has been derived as an active greenhouse gas reduction strategy, The focus of the system is beyond carbon neutral LFG utilization to neutral carbon absorption. The system is feasible in terms of direct and indirect $CO_2$ emission reduction with more economical way.

• Journal of Microbiology and Biotechnology
• /
• v.11 no.5
• /
• pp.772-775
• /
• 2001

A highly $CO_2$ tolerant microalga, Chlorella KR-1, has been isolated and used to fix $CO_2$ from actual flue gas. Growth of Chlorella KR-1 with the supply of flue gas from a liquified natural gas boiler was comparable to that obtained with 10% $CO_2$. Chlorella KR-1 produced from $CO_2$ fixation using the flue has about 50% crude protein with balanced amino acid profiles. Toxicity was not detected when the microalga was used as a feedstuff for chicks. These results indicate that the KR-1 cells could be a favorable protein source for poultry.

• Applied Biological Chemistry
• /
• v.11
• /
• pp.29-33
• /
• 1969

1. The oxidizability of $NADPH_2$ by quinones in the presence of $NADPH_2$-diaphorase was tested under aerobic conditions. Also the $^{14}CO_2$-fixation rates were compared when Chlorella suspensions were pretreated with $3{\cdot}10^{-5}M$ cocentration of variou quinones for 10 minutes prior and during the $^{14}CO_2$-fixation period. 2. A close correlation seems to exist between the rate of $NADPH_2$ oxidation by quinones and the $^{14}CO_2$-fixation rate. The effect of quinones on $NADPH_2$ oxidation and $^{14}CO_2$-fixation were in the order of Dichlone>06-K>NQ>BQ. 3. It is postulated that the phytotoxicity of quinones on Chlorella is due to the deprival of $NADPH_2$ consequently inhibiting $^{14}CO_2$-fixation, thus causing death of the cells. 4. The effect of quinones on amino acids biosyn-thesis in Chlorella was one of depressed rates, which was especially noted in the case of dichlone. This would be expected from a consideration of $NADPH_2$ deprival and inhibition of $^{14}CO_2$-fixation. Sucrose synthesis was either not affected or rather stimulated, the reasons of which are not clear at the present time.

• Journal of Environmental Science International
• /
• v.29 no.2
• /
• pp.123-132
• /
• 2020

Seasonal changes in the CO₂ fixation rate and water-use efficiency in the leaves of six evergreen and two deciduous broad-leaved tree species on Jeju Island, Korea, were measured using a portable photosynthesis analyzer, to identify which species are most efficient in taking up CO₂ from the air. The CO₂ fixation rate was high in the deciduous species in spring and summer and decreased in fall, whereas it was high in the evergreen species in summer and fall and decreased in winter. The rate remained high in the deciduous tree Prunus yedoensis from spring to fall (> 7.1 μmol CO₂/m₂/s) and in two evergreen trees, Castanopsis cuspidata var. sieboldii and Cinnamomum camphora, in summer and fall (7.0 9.9 μmol CO₂/㎡/s). Therefore, these tree species fix atmospheric CO₂ effectively. The water-use efficiency was higher in evergreen species than in deciduous species regardless of the season. Exceptionally, it was high in the deciduous species Zelkova serrata in spring and summer (> 100 μmol CO₂/mol H₂O), suggesting that Z. serrata is a useful tree for dry conditions due to its tolerance of water stress. The regressions of the CO₂ fixation rate versus the evaporation rate and stomatal conductance were linear and non-linear, respectively. This suggests that the stomatal activity of leaves plays an important part in CO₂ fixation of plants. In conclusion, C. cuspidata var. sieboldii, C. camphora, and P. yedoensis should be planted along roads or in urban spaces for the greening of cities and mitigation of CO₂ concentrations in the air.

• KSBB Journal
• /
• v.25 no.6
• /
• pp.559-564
• /
• 2010

A new hydrogen-oxidizing bacterium, Aeromonas sp. strain JS-1, that can fix $CO_2$ via the reductive pentose phosphate cycle (Calvin-Benson cycle) under chemoautotrophic conditions but not photoautotrophic conditions was isolated from fresh water. Strain JS-1 showed considerable $CO_2$ fixation ability during continuous cultivation even at high $CO_2$ concentration. Strain JS-1 used $H_2$ and $CO_2$ fixation as energy and carbon sources, respectively. Carbon dioxide fixation is carried out through the Calvin-Benson cycle, in which ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme. Hydrogen-oxidizing chemoautotrophic Aeromonas sp. strain JS-1 exhibited remarkedly strong RubisCO [EC 4.1.1.39] activity. RubisCO was purified as an $L_8S_8$-type hexadecamer with molecular mass of 560 kDa by gel filtration. The enzyme consisted of two different subunits eight large (56 kDa) and eight small (15 kDa), as demonstrated by SDS-PAGE. The specific activity of the purified enzyme was about 3.31 unit/mg and stable up to $45^{\circ}C$. The $K_m$ values for RuBP, $CO_2$, and $Mg^{2+}$ were estimated to be 0.25 mM, 5.2 mM and 0.91 mM, respectively.

• Microbiology and Biotechnology Letters
• /
• v.26 no.1
• /
• pp.1-6
• /
• 1998

The characteristics of $CO_2$ fixation by Chlorella HA-1 cultured in bubble columns were studied to achieve high photosynthetic rates per basal area. The influence of experimental conditions such as the diameter of a bubble column and gas flow rate, on photosynthesis of Chlorella HA-1 was investigated. The maximum productivity and the overall $CO_2$ fixation rate obtained in a 0.15 L bubble column was 1.09 g dry biomassa-day and 1048 g CO$_2/\m^2$-day, respectively. Light limitation has been observed in the bubble columns having a diameter larger than 3.5 cm.. As the reactor volume increased, the decrease of the $CO_2$ fixation rate was remarkable. High gas flow rate was helpful to mitigate the light limitation problem.

• Journal of Marine Bioscience and Biotechnology
• /
• v.16 no.1
• /
• pp.36-44
• /
• 2024

This study attempted to improve the growth of the freshwater microalgae, Parachlorella kessleri, through the sequential optimization of culture conditions. This attempt aimed to enhance the microalgae's ability to fixate atmospheric CO₂. Culture temperature and light intensity appropriate for microalgal growth were scanned using a high-throughput photobioreactor system. The supplied air flow rate varied from 0.05 to 0.3 vvm, and its effect on the growth rate of P. kessleri was determined. Next, sodium phosphate buffer was added to the culture medium (BG11) to enhance CO₂ fixation by increasing the availability of CO₂(HCO₃^-) in the culture medium. The results indicated that optimal culture temperature and light intensity were 20℃-25℃ and 300 μE/m²/s, respectively. Growth rates of P. kessleri under various air flow rates highly depended on the increase of the culture's flow rate and pH which determines CO₂ availability. Adding sodium phosphate buffer to BG11 to maintain a constant neutral pH (7.0) improved microalgal growth compared to control conditions (BG11 without sodium phosphate). These results indicate that the CO₂ fixation rate in the air could be enhanced via the sequential optimization of microalgal culture conditions.

• Journal of Bio-Environment Control
• /
• v.15 no.4
• /
• pp.364-368
• /
• 2006

This experiment was carried out to figure out the $CO_2$ biomass and the growth response of Chinese cabbage and radish grown under the condition of high temperature and high $CO_2$ concentration to provide the information for the coming climatic change. Chinese cabbage and radish were cultivated in spring and autumn seasons under 4 treatments, 'ambient temp.+ambient $CO_2$ conc.', 'ambient temp.+elevated $CO_2$ conc.', 'elevated temp.+ ambient $CO_2$ conc.', and 'elevated temp. +elevated $CO_2$ conc.'. The 'elevated temp,' plot was maintained at 5 higher than 'ambient temp. (outside temperature)'and the 'elevated $CO_2$ cone.' plot was done in 650 ppm $CO_2$. The growth of spring-sown Chinese cabbage was worse than autumn-sown one, and was affected more by high temperature than high $CO_2$. concentration. The $CO_2$ biomass of Chinese cabbage was lower as 25.1-39.1 g/plant in spring-sown one than 54.8-63.4 g/plant of autumn-sown one. Daily $CO_2$2 fixation ability was not significantly different between spring- and autumn-sown Chinese cabbage as 1.9-2.9, 2.7-3.1 kg/10a/day, respectively. The $CO_2$ biomass of radish were 87.4-104.6 /plant in spring-sown one and 51.3$\sim$76.4 g/plant in autumn-sown one. Daily $CO_2$ fixation ability of radish were 6.2-10.1 kg/10a/day in spring-sown one and 4.6-6.9 kg/10a/day in autumn-sown one.

• Journal of Wetlands Research
• /
• v.20 no.4
• /
• pp.363-369
• /
• 2018

One of the recent environmental problems is climate change due to the increase of atmospheric $CO_2$, which causes ecological changes and various environmental problems. Therefore, various studies are being carried out to reduce $CO_2$ in the world in order to solve various environmental problems caused by increase of $CO_2$. The $CO_2$ reduction using microalgae is an environmentally friendly method by using photosynthesis reaction of microalgae. However, most studies using single species. There is no study on the $CO_2$ fixing efficiency of microalgae in natural rivers. Therefore, this study was to identify the microalgae in the Sum river and to analyze the growth characteristics of microalgae in the river to obtain optimal culture conditions. And the changes of biomass and chlorophyll-a of microalgae were analyzed according to $CO_2$ concentration and injection rate. The purpose of this study was to investigate the fixing efficiency of carbon dioxide in microalgae in natural rivers. Six kinds of dominant species were observed as a result of the identification of microalgae in Sum river(Ankistrodesmus falcatus, Scenedesmus intermedius, Selenodictyum sp., Xanthidium apiculatum var. laeve, Cosmarium pseudoquinarium, Dictyosphaerium pulchellum). All of these species were green algae. Biomass and chlorophyll-a increased with the increase of $CO_2$ concentration and biomass and chlorophyll-a increased faster flow rate at the same $CO_2$ concentration. Also, the quantity of $CO_2$ fixation on the microalgae tended to be higher when the flow rate of injected gas was faster. This study can be referred as being significant in the micro-algae in river. In addition, the optimal conditions for $CO_2$ fixation of microalgae in rivers and the quantification of the quantity of $CO_2$ fixation from microalgae in rivers can be used as basic data for future policy of $CO_2$ reduction.

• KSBB Journal
• /
• v.15 no.6
• /
• pp.644-648
• /
• 2000

Biological fixation of carbon dioxide using microalgae is known as an effective CO$_2$reduction technology. However, many environmental factors influence microalgal productivity. Optimal cultivation factors were determined for the green alga, Euglena gracilis Z, which offers high protein and vitamin E content for animal fodder. In batch culture in a photovioreactor, it was found that theinitial pH, temperature, CO$_2$concentration in air, and light intensity during the optimal cultivating conditions were 3.5, 27$^{\circ}C$, 5-10% and 520 ${\mu}$mol/㎡/s, respectively. When tap water and freshwater were used as cultivating media unsterilized tap water was found to be effective. A kinetic model was considered to determine the relationship between the specific growth rate and the light intensity. The half-velocity coefficient (K(sub)I) in the Monod model under photoautotrophic conditions was 978.9 ${\mu}$mol/㎡/s.