• Korean Journal of Agricultural Science
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• v.47 no.1
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• pp.173-182
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• 2020

Human Astrovirus (HuAstV), known as a waterborne virus, is a group IV positive-sense single-stranded RNA that belongs to Astroviridae. The first outbreak of HuAstV was reported in England in 1975. HuAstV can exist not only among clinical patients but also in various water environments, such as water for agriculture and vegetables. For diagnosis of HuAstV from water samples, a polymerase chain reaction (PCR) system has been developed. However, the PCR-based diagnostic method has problems in field application, such as reaction time, sensitivity and specificity. For this reason, in this study we developed the loop-mediated isothermal amplification assay (LAMP) system, aimed specifically at HuAstV. Three prepared LAMP primer sets were tested by specificity, non-specificity and sensitivity; one LAMP primer set was selected with optimum reaction temperature. The developed LAMP primer set reaction conditions were confirmed at 62℃, and detection sensitivity was 1 fg/μL. In addition, restriction enzyme HaeIII (GG/CC) was introduced to confirm that the LAMP reaction was positive. As a result, selected LAMP primer set was 100 - 1000 times more specific, rapid, and sensitive than conventional-nested PCR methods. For verification of the developed LAMP assay, twenty samples of cDNA from groundwater samples were tested. We expect that the developed LAMP assay will be used to diagnose HuAstV from various samples.

• Korean Journal of Clinical Laboratory Science
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• v.53 no.3
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• pp.241-248
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• 2021

The present study was conducted to assess the relationship between aspartate aminotransferase/alanine aminotransferase (AST/ALT) ratio and pulse pressure in Korean adults with hypertension. Data from 1,515 adults from the sixth Korean National Health and Nutrition Examination Survey (KNHANES VI-3, 2015) were analyzed. There were several key findings in the present study. First, aspartate aminotransferase (odds ratio [OR], 1.018; 95% confidence interval [CI], 1.002 to 1.033), alanine aminotransferase (OR, 0.982; 95% CI, 0.969 to 0.996), and aspartate aminotransferase/alanine aminotransferase ratio (OR, 1.367; 95% CI, 1.027 to 1.819) were the independent factors determining high pulse pressure. Second, after adjusting for related variables [age, gender, smoking, alcohol consumption, regular exercise, total cholesterol (TC), triglycerides (TGs), high-density lipoprotein-cholesterol (HDL-C), fasting plasma glucose (FPG), body mass index (BMI), and waist circumference (WC)], the ORs of high pulse pressure with the 1st quartile as a reference were significantly higher in the 4th quartile of aspartate aminotransferase/alanine aminotransferase ratio [1.632 (95% CI, 1.113~2.393)]. The high pulse pressure was positively associated with aspartate aminotransferase and alanine aminotransferase/alanine aminotransferase ratio in Korean adults with hypertension, but was inversely associated with alanine aminotransferase.

• The Transactions of the Korea Information Processing Society
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• v.4 no.3
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• pp.831-845
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• 1997

In this paper,we proposed and developed a compiler and interactive programming enviroments for C++ wich is mostly worth of nitice among the object -oriented languages.To develope the compiler for C++ we took front=end/back-end model using EM virtual machine.In develpoing Front-End,we formailized C++ gram-mar with the context semsitive tokens which must be manipulated by dexical scanner and designed a AST class li-brary which is the hierarchy of AST node class and well defined interface among them,In develpoing Bacik-End,we proposed model for three major components :code oprtimizer,code generator and run-time enviroments.We emphasized the retargatable back-end which can be systrmatically reconfigured to genrate code for a variety of distinct target computers.We also developed terr pattern matching algorithm and implemented target code gen-erator which produce SPARC code.We also proposed the theroy and model for construction interative pro-gramming enviroments. To represent language features we adopt AST as internal reprsentation and propose uncremental analysis algorithm and viseal digrams.We also studied unparsing scheme, visual diagram,graphical user interface to generate interactive environments automatically Results of our resarch will be very useful for developing a complier and programming environments, and also can be used in compilers for parallel and distributed enviroments.

• Food Science of Animal Resources
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• v.25 no.2
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• pp.168-174
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• 2005

This study was carried out to investigate the feeding effect of wild grape wine by-products on pork qualities. The chemical composition, volatile basic nitrogen, thiobarbituric acid reactive substances and pH were not significantly different between control and wild grape pork, but the cholesterol and roast loss of wild grape polk. were lower than those of the control, and 1l1e salt soluble protein extractability of the control was lower than that of the wild grape pork. The Hunter's $a^{\ast}$ and $b^{\ast}$ value of wild grape polk. meat were higher than the control, the $a^{\ast}$ value of wild grape polk. meat was higher than the control, but the $L^{\ast}$ value of meat and fat were not significantly different between control and wild grape pork. The cohesiveness, gumminess and chewiness of control(respectively $66.2\%$ 428kg and 189g) were higher than wild grape polk. (respectively $61.4\%$ 357 kg and 154 g). The total amino acid composition of polk. were not significantly different between control and wild grape wine, The glutamic acid, leucine, arginine and aspartic acid were major amino acids in control and wild grape pork. The arachidonic acid $(C_{20:4})$, EPA $(C_{20:5})$ and DHA $(C_{22:6})$ of wild grape polk. were higher than those of the control. The taste (p<0.001), aroma (p<0.05), flavor (p<0.001), juiciness (p<0.01) and palatability (p<0.01) of wild grape polk. were higher than those of the control, but the texture between control and wild grape polk. were not significantly different.

• Korean Journal of Food Science and Technology
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• v.41 no.3
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• pp.265-271
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• 2009

The quality attributes of bread made with milk protein (casein, C; whey protein, W) and polysaccharide (sodium alginate, A; ${\kappa}$-carrageenan, K) mixtures were investigated to study the method to suppressing quality deterioration during storage. Bread prepared with the CA mixture had a higher specific loaf volume compared to the control. And bread made with the WA mixture had reduced moisture loss during storage compared to the control. The hardness of control and breads containing protein-polysaccharide mixtures increased during storage, but hardness increased more in the control than the treatments. In terms of crumb color, the breads containing protein-polysaccharide mixtures had higher $L^{\ast}$ values, but lower $a^{\ast}$ and $b^{\ast}$ values than the control. Finally, there were no significant differences in sensory quality among the control and treatment breads. Overall, data indicate that the addition of CA and WA improved the baking quality of bread and retarded staling.

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.16 no.6
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• pp.590-605
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• 1987

The purpose of this study was to investigate thermal environmental factors, thermal clothing properties, and thermal sensation of the office workers in four selected office buildings in Seoul, and to determine the effect of thermal environmental factors and clothing insulation to the thermal sensation of the subjects. The subjects selected from each office were 5 males and 5 females at a time. Thermal environmental factors(DBT, GT, RH, MRT, $ET^{\ast}$) and clothing variables such as clothing weight per body surface $area(g/m^2)$ and estimated clothing insulation values(clo) were significantly different among each seasons(p<0,001). Means of $ET^{\ast}$ and estimated clothing insulation values of each season were as follows; Winter; $20.84^{\circ}C$ $ET^{\ast}$ with 0.72 clo for male and 0.79 clo for female Spring and fall; $23.65^{\circ}C$ $ET^{\ast}$ with 0.59 clo for male and 0.68 clo for female Summer; $26.00^{\circ}C$ $ET^{\ast}$ with 0.47 clo for male and 0.53 clo for female. In comparison these data with ASHRAE Standard, the subjects were predicted to feel comfort-able in spring and fall, and slightly hot in summer and slightly cold in winter because of high and low clo respectively. But the result of this survey showed more than $80\%$ of the occupants were thermally comfortable at a given environmental temperature and clo.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.245-248
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• 2009

Recently, Many studies have been progressing to path-finding with dynamic information on the Terminal Based Navigation System(TBNS). However, the most of existing algorithms are based on $A{\ast}$ algorithm. Path-finding algorithms which use heuristic function may occur a problem of the increase of exploring cost in case of that there is no way determined by heuristic function or there are 2 way more which have almost same cost. In this paper, two abstract graph(AG) that are different method of construction, Homogeneous Node merging($AG^H$) and Connected Node Merging($AG^C$), are implemented. The abstract graph is a simple graph of real road network. The method of using the abstract graph is proposed for reducing dependency of heuristic and exploring cost. In result of evaluation of performance, $AG^C$ has better performance than $AG^H$ at construction cost but $AG^C$ has worse performance than $AG^H$ exploring cost.

• Honam Mathematical Journal
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• v.7 no.1
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• pp.119-127
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• 1985

A study is made of a special type of $C^{\ast}$ -dynamical systems, consisting of a class $n^{\infty}$ uniformly hyperfinite $C^{\ast}$-algebra A, the torus group $G=T^{d}$ ($$1{\leq_-}d{\leq_-}n-1$$) and a natural product action of G on A by $^{*}-automorphisms$. We give some conditions for product states on the fixed point algebra $A^{G}$ of A by G to be factorial.

• Korean Journal of Chemical Engineering
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• v.35 no.11
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• pp.2290-2295
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• 2018

An acceleration stress test (AST) was performed to evaluate the durability of a polymer membrane in a polymer electrolyte membrane fuel cell (PEMFC) for 500 hours. Previous studies have shown that hydrogen crossover measured by linear sweep voltammetry (LSV) increases when the polymer membrane deteriorates in the AST process. On the other hand, hydrogen crossover of the membrane often decreases in the early stages of the AST test. To investigate the cause of this phenomenon, we analyzed the MEA operated for 50 hours using the AST method (OCV, RH 30% and $90^{\circ}C$). Cyclic voltammetry and transmission electron showed that the electrochemical surface area (ECSA) decreased due to the growth of electrode catalyst particles and that the hydrogen crossover current density measured by LSV could be reduced. Fourier transform infrared spectroscopy and thermogravimetric/differential thermal analysis showed that -S-O-S- crosslinking occurred in the polymer after the 50 hour AST. Gas chromatography showed that the hydrogen permeability was decreased by -S-O-S- crosslinking. The reduction of the hydrogen crossover current density measured by LSV in the early stages of AST could be caused by both reduction of the electrochemical surface area of the electrode catalyst and -S-O-S- crosslinking.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.2
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• pp.129-134
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• 2014

Two neuropeptides were purified from the acidified tube feet extract of the starfish Asterias amurensis by C18 reversed phase and size-exclusion high-performance liquid chromatography (HPLC). The tube feet extract and the purified peptides (AST-I and AST-II) showed potent contractile activity on dorsal retractor muscle (DRM) of the starfish Asterina pectinifera and intestine (smooth muscle) of the panther puffer Takifugu pardalis. Treatment of the purified peptides with dithiothreitol (DTT) for 60 min at $37^{\circ}C$ significantly altered their retention times, suggesting that these compounds contained disulfide bonds. The molecular weights of AST-I and AST-II were determined to be 2064.2 Da and 6137.2 Da, respectively, by MALDI-TOF mass spectrometry.