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Effect of Silk Fibroin on the Protection of Alcoholic Hepatotoxicity in the Liver of Alcohol Preference Mouse

  • Kang, Gyung-Don;Lee, Ki-Hoon;Do, Sun-Gil;Kim, Chung-Sub;Suh, Jun-Gyo;Oh, Yang-Seok;Nahm, Joong-Hee
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.1
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    • pp.15-18
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    • 2001
  • Silk fibroin (SF) derided from the domestic silk worm, bombyx mori, is the natural protein and widely used as bio-functional materials as well as apparels. We studied the livers protective effect of SF from alcohol-induced hepatotoxicity in the alcohol preference mouse. To increase more absorption of SF in experimental animals, molecular weight of SF was lowered by 2N of HCI aqueous solution at 10$0^{\circ}C$ for 48 hrs. SF was added to liquid diet with alcohol and fed to the alcohol preference mice for 4 weeks. To assess the liver function, the concentration of alanine aminotransferase (AlT), aspartate aminotransferase (AST) and cholesterol present in either blood or liver tissue were measured. As compared with non-SF treated groups the SF-treated showed significantly low concentrations of ALT, AST, cholesterol and triacylglycerol values, respectively. Histopathological examination revealed that the extent of hepatocyte injury in the SF-treated group was reduced when it was compared with non SF-treated group. These results suggest that SF may have liver protective effects against alcohol-induced hepatotoxicity.

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Meta-analysis of factors predicting resistance to intravenous immunoglobulin treatment in patients with Kawasaki disease

  • Baek, Jin-Young;Song, Min Seob
    • Clinical and Experimental Pediatrics
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    • v.59 no.2
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    • pp.80-90
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    • 2016
  • Purpose: Studies have been conducted to identify predictive factors of resistance to intravenous immunoglobulin (IVIG) for Kawasaki disease (KD). However, the results are conflicting. This study aimed to identify laboratory factors predictive of resistance to high-dose IVIG for KD by performing meta-analysis of available studies using statistical techniques. Methods: All relevant scientific publications from 2006 to 2014 were identified through PubMed searches. For studies in English on KD and IVIG resistance, predictive factors were included. A meta-analysis was performed that calculated the effect size of various laboratory parameters as predictive factors for IVIG-resistant KD. Results: Twelve studies comprising 2,745 patients were included. Meta-analysis demonstrated significant effect sizes for several laboratory parameters: polymorphonuclear leukocytes (PMNs) 0.698 (95% confidence interval [CI], 0.469-0.926), C-reactive protein (CRP) 0.375 (95% CI, 0.086-0.663), pro-brain natriuretic peptide (pro-BNP) 0.561 (95% CI, 0.261-0.861), total bilirubin 0.859 (95% CI, 0.582-1.136), alanine aminotransferase (AST) 0.503 (95% CI, 0.313-0.693), aspartate aminotransferase (ALT) 0.436 (95% CI, 0.275-0.597), albumin 0.427 (95% CI, -0.657 to -0.198), and sodium 0.604 (95% CI, -0.839 to -0.370). Particularly, total bilirubin, PMN, sodium, pro-BNP, and AST, in descending numerical order, demonstrated more than a medium effect size. Conclusion: Based on the results of this study, laboratory predictive factors for IVIG-resistant KD included higher total bilirubin, PMN, pro-BNP, AST, ALT, and CRP, and lower sodium and albumin. The presence of several of these predictive factors should alert clinicians to the increased likelihood that the patient may not respond adequately to initial IVIG therapy.

The Characteristics and Change of Colors on Fashion Collections in 1990s

  • Kim, Honey;Kim, Young-In
    • International Journal of Costume and Fashion
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    • v.7 no.2
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    • pp.18-31
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    • 2007
  • The purpose of this study is to examine color characteristics and color changes of the fashion collections through 1990s, and to provide the efficient color information for color planning upon fashion themes. For this research, a total of 30,084 colors were collected from Paris, Milan, London, New York Collections in 1990s. Those colors were first measured by the Pantone Textile Color Specifier and COS Color System and spectrophotometer(color eye 580). These measured color values $L^{\ast}a^{\ast}b^{\ast}$of CIE were converted into H V/C of Munsell System, and 12 tones of PCCS with 5 achromatic colors. The characteristics of collected colors were analyzed in general and by place, season and year. The results of the study are as follows : First, the hues of purple blue, yellow red, red, yellow and the tones of grayish, pale, white, black, dark grayish, dull, light grayish appeared mostly. Yellow was shown quite frequently in spring/summer while purple, purple blue, red and yellow red in fall/winter. White, pale, light, light grayish and light gray were shown more frequently in spring/summer while Black, dark grayish, grayish, dark gray and dark in fall/winter. Second, the characteristics of colors by 4 representative places were similar to the general characteristics of colors in 1990's. Third, There were distributed widely Red, Yellow Red, Yellow in the early 1990s, Green Yellow, Green, Blue Green in the mid of 1990s, and Purple Blue, Purple in the late of 1990s. The distribution range of chromatic colors showed wide in both of the early of 1990s and the mid of 1990s for a while, and achromatic colors of grayish, gray and black appeared mostly in the late of 1900s.

Quality Characteristics of Kipfel Cookie Prepared with Chitosan-Chungkukjang (키토산 청국장을 첨가하여 제조한 깊펠 쿠키의 품질 특성)

  • Lee Ye-Kyung;Kim Mee-Jung;Lee Seung-Bae;Kim Soon-Dong
    • Journal of the East Asian Society of Dietary Life
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    • v.15 no.4
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    • pp.437-443
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    • 2005
  • Quality characteristic of Kipfel cookies with 0, 20, 40 and $60\%$ of freeze dried chitosan-chungkukjang(CC), which was fermented for 24 hours at $40^{\circ}C$ with steamed soybean added with $0.25\%$ of chitosan(MW 2,025 kDa) powder and inoculated $2\%$ of Bacillus lichenifomis, were investigated Bulk density was higher in the CC cookie, but there were no difference among the CC cookies. While hardness was decreased, brittleness and springiness were increased in the higher ratio of CC, but no significant difference was observed in cohesiveness and gumminess. According to increasing the CC ratio, L$\ast$ values and hue angle were decreased from 62.69 and 96.71 to 44.41 and 69.30, respectively. While a$\ast$ values were increased from -1.94 to 4.95, and no changes were observed in b$\ast$ values. Glucosamine content was $27.34 mg\%$ in the control cookie, $40{\~}93.75 mg\%$ in the CC cookies. Antioxidant activity of the CC cookies were higher than the control. The activity of the control cookie was decreased during storage, while it was maintained in CC cookies. There were no differences in the sour, sweet and savory taste. Off-flavor did not detected in the $0{\~}40\%$ CC cookies, but the chungkukjang odor was slightly detected in the $60\%$ CC cookie. The $20{\~}40\%$ CC cookies did not affect on native odor of Kipfel cookie. Color acceptability also did not affect by addition of CC $20{\~}40\%$ but it was lower in $60\%$ CC cookie. Overall acceptability was the best in the $20\%$ CC cookie.

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Non-enzymatic Antioxidant Status and Biochemical Parameters in the Consumers of Pan Masala Containing Tobacco

  • Shrestha, Raj;Nepal, Ashwini Kumar;Lal Das, Binod Kumar;Gelal, Basanta;Lamsal, Madhab
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.9
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    • pp.4353-4356
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    • 2012
  • Background: Tobacco consumption is one of the leading causes of oral submucous fibrosis, oral cancer and even premature death. The present study was designed to compare the biochemical parameters and non-enzymatic antioxidant status and the lipid peroxidation products in pan masala tobacco users as compared with age-matched non-user controls. Methods: Pan masala and tobacco users of age $33.2{\pm}9.94$ years and age-matched controls ($31.2{\pm}4.73$ years) were enrolled for the study. Plasma levels of vitamin E, vitamin C, albumin, bilirubin, uric acid, glucose, urea, creatinine, aspartate amino transferase (AST), alanine amino transferase (ALT) were measured by standard methods. Serum malondialdehyde (MDA) levels were estimated as a measure of lipid peroxidation. Results: In the pan masala tobacco users, as compared to the controls, the level of vitamin C ($68.5{\pm}5.9$ vs $97.9{\pm}9.03{\mu}mol/L$, $p{\leq}0.05$) vitamin E ($18.4{\pm}5.3$ vs $97.9{\pm}9.03{\mu}mol/L$, $p{\leq}0.001$), albumin ($37.5{\pm}7.01$ vs $44.3{\pm}9.99g/L$, $p{\leq}0.001$), and malondialdehyde ($10.8{\pm}1.29$ vs $1.72{\pm}1.15nmol/ml$, $p{\leq}0.001$) were found to be significantly altered. Malondialdehyde was significantly correlated with vitamin E (r=1.00, p<0.001) and vitamin C (r=1.00, p<0.001) in pan masala tobacco users. Serum levels of AST ($31.0{\pm}16.77$ IU) and ALT ($36.7{\pm}31.3$ IU) in the pan masala tobacco users were significantly raised as compared to the controls (AST, $25.2{\pm}9.51$ IU, p=0.038; ALT, $26.2{\pm}17.9$ IU, p=0.038). Conclusion: These findings suggest that pan masala tobacco users are in a state of oxidative stress promoting cellular damage. Non-enzymatic antioxidants are depleted in pan masala tobacco users with subsequent alteration in the biochemical parameters. Supplementation of antioxidants may prevent oxidative damage in pan masala tobacco users.

Assessment of Validity of RT-PCR and EIA for The Detection of Hepatitis C Virus Infection (C형 간염의 진단을 위한 역전사중합효소연쇄반응과 효소면역측정법의 타당성 평가)

  • Son, Byung-Chul;Chun, Jin-Ho;Park, Yeong-Hong;Shin, Hai-Rim;Cho, Kyu-Il;Kim, Jong-Han;Jung, Kui-Oak;Lee, Jong-Tae;Lee, Chae-Un;Paik, Nak-Whan
    • Journal of Preventive Medicine and Public Health
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    • v.28 no.2 s.50
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    • pp.526-541
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    • 1995
  • This study was conducted to estimate the validity of reverse transcriptase-polymerase chain reaction(RT-PCR) compared to enzyme immunoassay(EIA) for the detection of hepatitis C virus (HCV) infection. EIA for antibody to HCV(anti-HCV) and RT-PCR for HCV was executed on the subjects from Pusan and Kyungnam area with questionnaire survey to collect some relating factors of HCV infection. As the result from 617 cases, the prevalence of HCV infection was 1.5% by EIA and 3.7% by RT-PCR(p<0.05), and the age standardized rate was 1.7% and 3.4% by EIA and RT-PCR, respectively. The prevalence of hepatitis B surface antigen(HBsAg) was 6.8% by enzyme linked immunosorbent assay(ELISA) and the age standardized rate was 7.7%. It was the higher in male group comparing to female group(p<0.01). Both of the prevalence of HCV and HBsAg were higher in elevated asparate aminotransferase(AST) and alanine aminotransferase (ALT) group than in normal AST and ALT group(p<0.01). There was no specific risk factor of HCV infection. Though the degree of agreement of EIA and RT-PCR by gamma statistics was 97.2%, it showed a significant difference between the two methods(p<0.01). For the detection of HCV infection, positive predictive value of EIA was 66.7% and negative predictive value of EIA was 97.2%. This study suggests that negative result to anti-HCV by EIA didn't mean the free state of HCV infection, therefore it would be helpful that further monitoring for HCV infection by RT-PCR in the case of elevated AST and ALT and/or clinically suspected.

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Effects of Ginseng Powder Additives on Quality Characteristics of Press Ham (인삼분말 첨가가 프레스햄 품질 특성에 미치는 영향)

  • Lee, Jeong-Ill;Ha, Young-Joo;Jung, Jae-Doo;Lee, Jin-Woo;Lee, Jae-Ryung;Do, Chang-Hee;Lee, Jung-Dong
    • Food Science of Animal Resources
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    • v.25 no.3
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    • pp.277-284
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    • 2005
  • Press ham were manufactured to investigate the effects of ginseng powder on quality characteristics of press ham. Each treatments added pork loin basis with ginseng powder(0, 0.5, 1.0, 1.5 and $2.0\%$) were stored until 28 days at $4^{\circ}C$. The changes in physico-chemical properties, texture and chemical composition of each treatments were measured during 1, 7, 14, 21 and 28 days at $4^{\circ}C$. There was a not significantly difference in chemical composition between control and ginseng treatment groups. pH value of ginseng treatment groups were decreased significantly than those of control(p<0.05). pH of control and ginseng treatments were increased significantly as the storage period passed(p<0.05). Meat color(CIE $L^{\ast},\;b^{\ast}$) of ginseng treatment groups were increased significantly than that of control(p<0.05). Meat color(CIE $a^{\ast}$) of ginseng treatment groups were decreased significantly than those of control(p<0.05). It was not clearly changed by the passage of storage time. There was a not significantly difference in texture between control and ginseng treatment groups. It was not clearly changed by the passage of storage time. Summing up the a forementioned result, press ham manufacturing with ginseng powder was not affected in physico-chemical properties and texture characteristics. Also, it may be assumed that the high quality press ham can be manufactured with saponin accumulation.

Hepatoprotective Activity of Dandelion (Taraxacum officinale) Water Extract against D-Galactosamine-Induced Hepatitis in Rats (D-Galactosamine으로 유발된 간손상에 대한 민들레 열수추출물의 예방효과)

  • Park, Ji-Young;Park, Chung-Mu;Kim, Jin-Ju;Song, Young-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.2
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    • pp.177-183
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    • 2008
  • This study aimed to investigate the protective effect of dandelion water extract (DWE) on liver injury induced by D-galactosamine (GalN) in Sprague-Dawley rats. Fifty rats were divided into 5 groups; normal control (C), DWE-control (DWE-C: saline injection after feeding 3% DWE diet), GalN-control (GalN-C: GalN injection after normal diet), DWE I (GalN injection after feeding 1.5% DWE diet), and DWE II (GalN injection after feeding 3% DWE diet). After 2 weeks, the acute hepatitis was induced by GalN (650 mg/kg, i.p.) and 24 hrs later, all rats were sacrificed. The DWE supplement ameliorated the serum alanine and aspartate aminotransferase (AST, ALT) as well as alkaline phosphatase (ALP) and tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$. Hepatic antioxidative enzyme activities, such as catalase, GSH peroxidase, GSH reductase, and Mn-superoxide dismutase (SOD) were slightly or significantly elevated by the treatment of DWE. Moreover, the histological examination corresponded with these biochemical observations. According to these findings, dandelion could be used as a potential therapeutic material for treating chemically induced acute hepatitis.

Synthesis of Ferroelectric Liquid Crystals Obtained from Optically Active l-isoleusine (광학활성 이소로이신을 출발물질로 한 강유전성 액정물질의 합성)

  • Jeong, N.H.;Kikuchi, H.;Kajiyama, T.
    • Journal of the Korean Applied Science and Technology
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    • v.13 no.3
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    • pp.51-60
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    • 1996
  • A new one series of chiral schiff's base containing benzylidene aniline moieties was synthesized and characterized by IR, $^1H$ NMR. elementary analysis, and polarized optical microscopy(POM). The yield of these synthetic compounds was in the range of $62{\sim}67^{\circ}C$. The results showed that most of the synthetic compounds were monotropic liquid crystal and exhibited chiral smectic C($Sc^{\ast}$) liquid crystal phases, and the range of phase transition temperature was to $71.4^{\circ}C$ from $45.0^{\circ}C$.

A Periodic Replacement Model with Random Repair Costs and Threshold Levels (확률적 수리비용과 임계수준을 고려한 주기적 교체 모형에 관한 연구)

  • Gang Yeong-Gil;Gang Seong-Jin
    • Journal of the military operations research society of Korea
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    • v.18 no.2
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    • pp.114-125
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    • 1992
  • A policy of periodic replacement with minimal repair at failure is considered for a complex system. Under such a policy the system is replaced at periodic times. iT(i=1,2, $\ldots$), while minimal repair is performed at any intervening system failures. The cost of the j-th minimal repair to the component which fails at age t is g(C(t). $c_j$ (t)), where C(t) is the age-dependent random part, $c_j$(t) is the deterministic part which depends on the age and the number of the minimal repair to the component, and g is a positive nondecreasing continuous function. The cost of replacement is expensive when the number of failures occurring in (0. T) is greater than a threshold level. The problem of determining the optimal replacement period, $T^{\ast}$, which minimizes the total expected cost per unit time over an infinite time horizon is considered. Various special cases are considered.

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