• Journal of Animal Science and Technology
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• 제48권6호
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• pp.805-812
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• 2006

본 연구의 목적은 소 체외수정란의 체외발달에 있어서 Matrigel의 첨가효과를 조사하는 것이었다. 소 체외수정란은 Ⅰ) SOF+0.8% BSA (SOF-B), Ⅱ) 0.8% Matrigel이 첨가된 SOF+0.8% BSA (SOF-M), 그리고 Ⅲ) SOF+0.8% BSA 배양 후, SOF+10% FBS(SOF-BF)에서 배양을 실시하였다. 배양액 내 Matrigel의 첨가는 배반포 발달율을 증가시키지 않는 것으로 나타났다(SOF-B, 26.6%; SOF-M, 28.2%; SOF-BF, 26.2%). 그러나, 부화 배반포의 비율은 Matrigel 첨가에 의하여 유의하게 증가하는 것으로 나타났다(SOF-B, 23.7%; SOF-M, 48.7%; SOF-BF, 18.5%) (P<0.05). 배반포의 세포수를 조사한 결과는 각 처리군 간에 차이가 없는 것으로 나타났으며(SOF-B, 172.7±35.5; SOF-M, 175.1±37.4; SOF-BF, 172.8±38.1), 또한 세포자연사가 유발된 세포수의 비율도 각 처리군 간에 차이가 없는 것으로 조사되었다(SOF-B, 3.6±3.2%; SOF-M, 4.3±2.6%; SOF-BF, 4.9±4.3%). 본 실험결과는 Matrigel이 소 체외수정란의 부화과정을 촉진시키는 것으로 나타났다. 이상의 결과들을 종합해 볼 때, 세포외 기질 복합체인 Matrigel의 첨가는 무혈청 수정란 배양체계에서 보다 생리적인 환경을 조성할 수 있는 방법이 될 수 있을 것으로 사료된다.

• Advances in Traditional Medicine
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• 제8권4호
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• pp.339-348
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• 2008

Butea frondosa has been used traditionally as a topical formulation in the treatment of many diseases and disorders. Two compounds [BF-1 (crystalline flavonol quercetin) and BF-2 (tannin) from ethyl acetate fraction of ethanolic extract] were isolated from the bark of Butea frondosa. The stereostructures of the compounds were determined on the basis of chemical and physicochemical evidence. BF-1 and BF-2 were screened in vitro for possible antibacterial property against 112 bacteria comprising 3 genera of Gram-positive and 12 genera of Gram-negative types. It was found that both BF-1 and BF-2 exhibited inhibitory activity against several bacteria. Most of these strains were inhibited by BF-1 at $50-200\;{\mu}g/ml$, while BF-2 ($MIC_{50}$ $400\;{\mu}g/ml$) was much less active. The bacteria could be arranged in the decreasing order of sensitivity towards BF-1 in the following manner: S. aureus, Bacillus spp., Salmonella spp., Vibrio spp., Shigella spp., E. coli and Pseudomonas spp. The $MIC_{50}$ of the compound was $50\;{\mu}g/ml$ while the $MIC_{90}$ was $100\;{\mu}g/ml$. The decreasing order of sensitivity towards BF-2 was V. cholerae, Bacillus spp., S. aureus, V. parahaemolyticus, Salmonella spp. and Proteus spp. BF-1 was bactericidal in action. In vivo studies with this extract showed that it could offer statistically significant protection (p < 0.01) to mice challenged with a virulent bacterium. The inhibitory activity of Butea frondosa against Gram-positive and Gram-negative bacteria indicates its usefulness in the treatment of common bacterial infections. The potentiality of BF-1 as an antibacterial agent may be confirmed further by pharmacological studies.

• 대한한의학회지
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• 제29권3호
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• pp.113-123
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• 2008

Objectives: Obesity is chronic condition which can severely influence health. People with a high percentage of body fat (%BF) have high prevalence of hypertension and hypercholesterolemia. Abdominal obesity (AO) seems to play a major role in the development of cardiovascular disease, diabetes and hyperlipidemia. This study investigated the %BF and AO of workers in the automobile manufacturing industry. Methods: The subjects were categorized by sex, age, job class, drinking history and smoking history. %BF and AO were measured by using bioelectrical impedance analysis (Jawon Medical, Seoul, Korea). We analyzed the relationship between age, sex, job class, drinking history, smoking history and %BF and WHR into frequencies, $X^2$-test using the SPSS Ver. 12.0. Results: Regarding the relationship between sex and %BF, AO, there was statistically significant difference as men had a significant higher level of %BF and AO than women. There was a positive correlation between age and both %BF and AO. Workers in their 40s especially ran level of AO up. In job class, office workers were apt to have a higherlevel of %BF and AO than production line workers. %BF and AO of the non-drinker group showed a lower level than the drinker group. On the other hand, the ex-smoker group had higher levels of %BF and AO than the non-smoker or current-smoker groups. Conclusions: The above results showed that men, the advanced in years, office workers, drinkers and ex-smokers had high levels of %BF and AO.

• 공업화학
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• 제26권5호
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• pp.615-620
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• 2015

폴리프로필렌(polypropylene, PP)과 대나무 섬유(bamboo fiber, BF) 복합체의 물성에 미치는 상용화제의 영향을 고찰하기 위해 이축압출기를 이용하여 PP/BF 복합체를 제조하였다. BF의 함량은 10에서 25 wt%로 변량하였고, 상용화제는 3 wt%로 고정하였으며, 폴리프로필렌과 대나무 섬유와의 혼화성 증대를 위한 상용화제는 무수말레인산(maleic anhydride, MAH)이 그라프트된 PP-g-MAH를 사용하였다. 상용화제를 적용한 복합체의 화학구조는 적외선 분광 스펙트럼의 $1700cm^{-1}$ 근처에서 나타나는 카르보닐기(C=O) 신축진동 피크의 존재 여부를 통해 확인하였으며, 압출온도와 스크류 회전속도는 기계적 물성과 탄화 정도를 고려하여 210, 100 rpm으로 선정하였다. PP/BF 복합체의 용융거동은 큰 차이를 보이지 않았지만 결정화 온도는 $10-20^{\circ}C$ 정도 증가를 나타내었고, 이는 BF가 불균일 핵제로 작용하기 때문으로 해석할 수 있다. 인장시험, 굴곡시험을 통해 BF의 함량이 15-20 wt%일 때 상용화제의 효과가 뚜렷하게 나타나는 것을 확인하였고, 이는 PP와 BF의 계면 접착특성으로 설명할 수 있다. 계면 접착특성 향상은 파단면의 SEM 사진과 접촉각을 통해 확인하였다.

• 한국자원식물학회지
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• 제33권4호
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• pp.271-278
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• 2020

이상의 연구 결과로 먹넌출 열매 추출물은 GSK3β 의존성 Cyclin D1 단백질의 분해를 통해 대장암세포의 생육 억제와 관련이 있는 것으로 확인된다. 본 결과는 대장암의 항암제 개발을 위한 소재로 먹넌출 열매의 활용이 가능할 것으로 판단된다.

• Journal of Applied Biological Chemistry
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• 제62권3호
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• pp.229-237
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• 2019

More effective treatments are needed for non-alcoholic fatty liver disease (NAFLD). We hypothesized that water extracts of blackberry fruits (BF) and leaves (BL) and their combinations (BFL) reduce fat deposition in HepG2 cells and modulate shor-tchain fatty acids (SCFA) and fecal bacteria in vitro. HepG2 cells were treated with BF, BL, BFL1:2, and BFL1:3 for 1 h, and 0.5 mM palmitate was added to the cells. Moreover, low ($30{\mu}g/mL$) and high doses ($90{\mu}g/mL$) of BL and BF were applied to fecal bacteria in vitro, and SCFA was measured by GC. BL, BF, BFL1:2, and BFL1:3 reduced triglyceride deposition in the cells in a dose-dependent manner, and BFL1:2 and BFL1:3 had a stronger effect than BF. The content of malondialdehyde, an index of oxidative stress, was also reduced in BL, BF, and BFL1:2 with increasing superoxide dismutase and glutathione peroxidase activities. The mRNA expression of acetyl CoA carboxylase, fatty acid synthase, and sterol regulatory element-binding protein-1c was reduced in BL, BF, BFL1:2, and BFL1:3 compared to the control, and BFL1:2 had the strongest effect. By contrast, the carnitine palmitolytransferase-1expression, a regulator of fatty acid oxidation, increased mostly in BFL1:2 and BFL1:3. Tumor necrosis factor-${\alpha}$ and interleukin-$1{\beta}$ expression was reduced in BL compared to that in BF and BFL1:2 in HepG2 cells. Interestingly, BL increased propionate production, and BF increased butyrate and propionate production and increased total SCFA content in fecal incubation. BF increased the contents of Bifidobacteriales and Lactobacillales and decreased those of Clostridiales, whereas BL elevated the contents of Bacteroidales and decreased those of Enterobacteriales. In conclusion, BFL1:2 and BFL1:3 may be potential therapeutic candidates for NAFLD.

• 대한방사선기술학회지:방사선기술과학
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• 제41권6호
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• pp.611-616
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• 2018

Lead apron is harmful to the human body because it is made at heavy metals, and when worn for long periods of time, it causes pain. Therefore, this paper intended to improve the defects of lead apron by using new material shields. For the comparative evaluation of lead and new material shieldes, the shielding rate and weight were measured and tested based on lead 0.5 mmPb. The rate of shielding was 97% based on lead at 0.5 mm thickness, while The new material shield T3 showed similar shielding rates as lead in 8 layers, and in T2 these values were measured in 11 layers. In addition, similar shielding rate was measured in 12 layers at B2, and 8 layers in BF, and 4 layers in $BF_2$. Comparing the weight of cases when commercialized with apron, T3, T2 and B2 were heavier than lead apron. But BF, $BF_2$ were lighter than the lead apron. Based on the results of the experiment, T3 and T2 can be used as an alternative to lead if human or environmental hazards are considered a priority. However, BF and $BF_2$ should be used if the reduction of external exposure is considered a priority.

• 한국축산식품학회지
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• 제29권2호
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• pp.278-282
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• 2009

Previous studies indicate that BF-7 enhances learning and memory in normal and elderly individuals. Here, we evaluated whether milk containing BF-7 (BF-7 milk) could improve the brain function, with thirty normal university students $(21{\pm}1.2 years)$. Two versions of the Paced Auditory Serial Addition Test were used under double-blinded conditions to measure the efficacy of BF-7 milk on learning and memory, especially working memory and attention, and on mathematical ability. As a result, BF-7 milk improved the accuracy of the task more than 3-fold. Furthermore, BF-7 milk protected cultured neuronal cells from 3-hydroxykynurenine, a normal endogenous brain stress agent. These results indicate that BF-7 milk enhances memory, attention and mathematical ability in normal persons.

• 상하수도학회지
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• 제35권4호
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• pp.277-284
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• 2021

The ballasted flocculation effects of the mill scale and magnetite on activated sludge were investigated. Both ballasted flocculants (BF) could remarkably improve the sludge settleability in terms of zone settling velocity (ZSV) and sludge volume index (SVI). With the BF dosage of 0.2 to 2.0 g-BF/g-SS, the magnetite particles showed better efficiency on improving settling behavior of activated sludge than the mill scale due to higher surface area and hydrophobic property. The efficiency of SVI₃₀ with magnetite injection was 2.5 to 11.3% higher than mill scale injection and that of the ZSV appreciated from 23.7% to 44.4% for magnetite injection. Averaged floc size of the BF sludge with magnetite dosage (0.5 g-BF/g-SS) was 2.3 times higher than that of the control sludge. Dewaterability of the sludge was also greatly improved by addition of the BF. The specific resistance to filtration (SRF) was reduced exponentially with increasing the dosage of BF. However, the BF's particle size effect on the SRF looks to be marginal. Consequently, for improving the dewaterability, the BF played a physical role to remove the pore water of the biological flocs by intrusive attachment and a chemical role to induce aggregation of the flocs by charge neutralization.

• Bulletin of the Korean Chemical Society
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• 제8권3호
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• pp.140-144
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• 1987

The effect of Lewis acids on spectroscopic properties and photodimerization of coumarin and 5,7-dimethoxycoumarin was investigated. Quantum yields of coumarin photodimerization increase in the presence of $BF_3{\cdot}OEt_2$ but those of 5,7-dimethoxycoumarin decrease. The spectroscopic properties of the coumarin-$BF_3{\cdot}OEt_2$ and 5,7-dimethoxycoumarin-$BF_3{\cdot}OEt_2$ complexes were studied by UV, IR, $^1H$ NMR and fluorescence spectroscopy.