• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1996년도 춘계학술대회
• /
• pp.180-180
• /
• 1996

Previous studies have shown that kainic acid (KA) causes an elevation of hippocampal proenkephalin mRNA level. However, the role of proto-oncogene products, such as c-Fos, c-Jun and Fra proteins in the regulation of KA-induced proenkephalin mRNA increase in the hippocampus has not been well characterized. Thus, in the present study, the effect of cycloheximide (CHX) on KA-induced proenkephalin mRNA and immediate early gene products induction was examined. After pretreating with either vehicle or CHX (20 mg/kg, s.c.) for 30 min, KA (10 mg/kg) was administered s.c. The animals were sacrificed 1,2, or 8 hrs after KA administration. Total RNA and were isolated for Northern blot assay, and proteins were isolated for Western and electrophoretic gel-shift assays. First, we found that CHX inhibited KA-induced proenkephalin mRNA increase without altering intracellular proenkephalin protein level. Secondly, Western blot assays showed that KA increased c-Fos, c-Jun and Fra proteins at 1,2, and 8 hrs and CHX inhibited these immediate early gene products. Finally, electrophoretic gel shift assays revealed that KA increased both AP-1 and ENKCRE-2 DNA binding activities. Furthermore, CHX attenuated KA-induced AP-1 and ENKCRE-2 DNA binding activities. Both AP-1 and ENKCRE-2 DNA binding activities were abolished by cold AP-1 or ENKCRE-2 oligonucleotides, and further reduced by antibodies against c-Fos or c-Jun. Antibody against CREB reduced ENKCRE-2, but not AP-1, DNA binding activity. Our results suggest that on-going protein synthesis is required for elevation of hippocampal proenkephalin mRNA level induced by KA. All c-Fos, c-Jun, and Fra proteins appears to be involved in the regulation of hippocampal proenkephalin mRNA level induced by KA (This study was supported by a grant from KOSEF).

• Archives of Pharmacal Research
• /
• 제25권3호
• /
• pp.375-380
• /
• 2002

Cytochrome P4501A2 (CYP1A2) is a member of the cytochrome P450 family of isozymes involved in the phase I drug metabolism of vertebrates. CYP1A2 is responsible for the activation of a number of aromatic amines to mutagenic and carcinogenic forms. Thus, the level of CYP1A2, which varies among different populations, may determine an individual's susceptibility to these chemicals. We have previously reported on the importance of a cis element named PRB (protected region B) in the regulation of human Cytochrome P4501A2 (CYP1A2) gene, which appeared to act as a positive regulatory element. Closer examination of the PRB sequence (-2218 to -2187 bp) revealed a putative AP-1 binding site, TGACTAA, at -2212 bp (Chung and Bresnick, 1997). To elucidate the role of AP-1 in CYP1A2 regulation, we transiently overexpressed c-Jun and c-Fos transcription factors in human hepatoma HepG2 cells, and examined their influence on the CYP1A2 promoter activity by reporter gene assays. Cotransfection of the c-Jun and the c-Fos expression vectors increased the induced transactivation by five to six fold from the CYP1A2 promoter constructs. However, deletion of the PRB element did not affect the degree of activation by the c-Jun and the c-Fos. Therefore, it is unlikely that the c-Jun and the c-Fos activate the CYP1A2 promoter through this AP-1 consensus-like sequence in the PRB region.

• 한국식품과학회지
• /
• 제28권4호
• /
• pp.650-656
• /
• 1996

절임배추를 효과적으로 저장 유통할 수 있는 방법을 개발하기 위하여 포장압력(상압, 감압)과 저장온도 $(0^{\circ}C,\;20^{\circ}C)$를 달리하여 저장하면서 절임배추의 품질특성을 외관, 수율, 염도, pH, 환원당 함량, 텍스쳐 및 즙액용출량 등으로 조사한 결과, 수율과 염도는 상압포장 하여 $0^{\circ}C$로 저장한 것이 1주 저장 후 수율이 87.1%로 가장 높았고, 염도변화와 pH변화도 가장 작았다. 그러나 환원당 함량은 감압포장하여 $0^{\circ}C$ 저장한 경우에 배추즙액의 용출이 많아서 상대적으로 환원당 함량이 가장 높았다. 외관과 즙액 용출량은 상압포장하여 $0^{\circ}C$에서 저장하는 경우 조직액의 용출이 128.9ml/kg으로 가장 적었고, 텍스쳐도 상압포장하여 $0^{\circ}C$에 저장 한 경우에 압축강도의 변화가 가장 작았다. 이상의 결과에서 상압포장하여 저온저장하는 경우 절임배추의 특성 변화가 적어 절임배추의 포장 유통방법으로 적합한 것으로 판단된다.

• 대한화학회지
• /
• 제31권4호
• /
• pp.352-358
• /
• 1987

$25^{\circ}C$의 1 : 4 dioxane-물의 혼합용액속에서 파라-치환된 phenyl N-(p-chlorobenzoyl)chloroformimidate (I) 유도체들의 가수분해 반응속도 상수를 측정하고 반응속도식, 치환기 효과$(\rho\>{\rho}^+)$, 생성물 분석 및 분자궤도 함수의 계산 결과로부터 pH3.0 이하에서는 azocarbonium 이온(II)이 생성되는 $S_N1$반응 메카니즘으로 무촉매 반응이 일어나며, pH 4.0이상에서는 전이상태(III)를 지나는 $S_N2$반응 메카니즘을 통하여 염기 촉매반응이 일어남을 제안 할 수 있었다. 4가지 peri planar형태 이성질체들의 상대적인 안정도는 각각 (E-ap) > (Z-ap) > (E-sp) > 및 (Z-ap)이었고, (E-ap)형태의 가장 안정한 입체구조는 benzimidochloroformyl group면에 대하여 Y-치환 phenyl group이 수직$(90^{\circ})$을 이루었으며 (I)의 활성화된 azomethine탄소 원자에 대하여 물분자는 시그마 공격에 의하여 친핵성 반응이 일어난다.

• 한국의류학회지
• /
• 제30권2호
• /
• pp.255-265
• /
• 2006

The purpose of this study was to examine the dyeing properties of four kinds of composite yams that were twisted in different processes. The composite yarns consist of acetate and functional polyester in ratio of 70 : 30. The composite yams were dyed at 100$^{circ}C$ and 125$^{circ}C$ using three types of dyes, disperse dyes for acetate fiber, PET fiber and PET-acetate blended fiber, in the three primary colors. The exhaustion($\%$) and K/S value were observed for each case. Also the effects of four different twisting processes to dyeing properties and physical properties were examined. Regardless of twisting methods, the composite yarns that were dyed at 125$^{circ}C$ had higher exhaustion($\%$) than those were dyed at 100$^{circ}C$ with all three types of dyes; However, tendency of the K/S value after reduction cleaning process was measured at 100$^{circ}C$ and the value measured 125$^{circ}C$ had a great difference with disperse dyes for acetate and dyes for PET. The difference of the K/S values of composite yarns, when dyed at 100$^{circ}C$ and 125$^{circ}C$ with disperse dyes for PET-acetate blended fiber, was almost negligible. According to twisting methods K/S values were in the following order: AP1 > AP3 > AP4> AP2. This means that AP1, treated at 220$^{circ}C$, had the highest K/S value and K/S value becomes higher as the yam is higher twisted and becomes lower as lower twisted. On the other hand, the dry shrinkage and wet shrinkage showed low shrinkage rate when the twist was high and steam setting temperature was high.

• Journal of Ecology and Environment
• /
• 제42권1호
• /
• pp.20-29
• /
• 2018

Background: For various reasons such as agricultural and economical purposes, land-use changes are rapidly increasing not only in Korea but also in the world, leading to shifts in the characteristics of local carbon cycle. Therefore, in order to understand the large-scale ecosystem carbon cycle, it is necessary first to understand vegetation on this local scale. As a result, it is essential to comprehend change of the carbon balance attributed by the land-use changes. In this study, we attempt to understand accumulated soil carbon (ASC) and soil respiration (Rs) related to carbon cycle in two ecosystems, artificially turned forest into pastureland from forest and a native deciduous temperate forest, resulted from different land-use in the same area. Results: Rs were shown typical seasonal changes in the alpine pastureland (AP) and temperate deciduous forest (TDF). The annual average Rs was $160.5mg\;CO_2\;m^{-2}h^{-1}$ in the AP, but it was $405.1mg\;CO_2\;m^{-2}h^{-1}$ in the TDF, indicating that the Rs in the AP was lower about 54% than that in the TDF. Also, ASC in the AP was $124.49Mg\;C\;ha^{-1}$ from litter layer to 30-cm soil depth. The ASC was about $88.9Mg\;C\;ha^{-1}$, and it was 71.5% of that of the AP. The temperature factors in the AP was high about $4^{\circ}C$ on average compared to the TDF. In AP, it was observed high amount of sunlight entering near the soil surface which is related to high soil temperature is due to low canopy structure. This tendency is due to the smaller emission of organic carbon that is accumulated in the soil, which means a higher ASC in the AP compared to the TDF. Conclusions: The artificial transformation of natural ecosystems into different ecosystems is proceeding widely in the world as well as Korea. The change in land-use type is caused to make the different characteristics of carbon cycle and storage in same region. For evaluating and predicting the carbon cycle in the vegetation modified by the human activity, it is necessary to understand the carbon cycle and storage characteristics of natural ecosystems and converted ecosystems. In this study, we studied the characteristics of ecosystem carbon cycle using different forms in the same region. The land-use changes from a TDF to AP leads to changes in dominant vegetation. Removal of canopy increased light and temperature conditions and slightly decreased SMC during the growing season. Also, land-use change led to an increase of ASC and decrease of Rs in AP. In terms of ecosystem carbon sequestration, AP showed a greater amount of carbon stored in the soil due to sustained supply of above-ground liters and lower degradation rate (soil respiration) than TDF in the high mountains. This shows that TDF and AP do not have much difference in terms of storage and circulation of carbon because the amount of carbon in the forest biomass is stored in the soil in the AP.

• 한국의류산업학회지
• /
• 제10권3호
• /
• pp.394-398
• /
• 2008

One of barely water soluble ketones, acetophenone (AP) was dissolved in methanol and then was mixed with aqueous solution of C. I. Red Acid 114. In order to find out the role of AP in the dyeing process the rate constants and the activation parameters were calculated. The rate for the dyeing with AP was faster than that without it. Because of the reduced temperature dependence by AP the activation energy ($E_a$) for the dyeing with AP was smaller than that without it. With increasing temperature the activation enthalpy (${\Delta}H^*$), the activation entropy (${\Delta}S^*$), and the activation free energy ($G^*$) decreased, which was more noticeable in dyeing with AP. The rate constants and the activation parameters agreed well with the results from the previous reports that the ability of AP to increase disaggregation of dye molecules, loosening the wool fiber, and wickabilty of dyeing solution made it possible to dye wool fiber at low temperature.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권1호
• /
• pp.75-85
• /
• 2012

An experiment was conducted to compare the effect of the same amount of 18:2 offered either as 18:2n-6 or as a mixture of unprotected 18:2c9t11 and 18:2t10c12 on feed intake, milk components as well as plasma and milk fatty acid profile. Fifteen cows were blocked by milk yield and milk fat percentage and within block assigned randomly to 1 of 3 treatments (n = 5). Each cow passed a 12-d adjustment period (AP) on a basal diet. After the AP cows received 1 of 3 supplements during an 18-d experimental period (EP). The supplements contained either 1.0 kg ground sunflower seeds (S), 0.5 kg conjugated linoleic acid (CLA)-oil (C) or 0.75 kg of a mixture of ground sunflower seeds and CLA-oil (2:1; SC). All 3 supplements contained the same amount of 18:2 either as CLA (${\Sigma}18$:2c9t11+18:2t10c12, 1:1) or as 18:2c9c12. During the last 2 d of AP and the last 4 d of EP feed intake and milk yield were recorded daily and milk samples were collected at each milking. Blood samples were collected from the jugular vein on d 11 of AP and d 15 and 18 of EP. The 18:2 intake increased in all treatments from AP to EP. Regardless of the amount of supplemented CLA, the milk fat percentage decreased by 2.35 and 2.10%-units in treatment C and SC, respectively, whereas in the treatment S the decrease was with 0.99%-unit less pronounced. Thus, C and SC cows excreted daily a lower amount of milk fat than S cows. The concentration of trans 18:1 in the plasma and the milk increased from AP to EP and increased with increasing dietary CLA supply. While the concentration of 18:2c9t11 and 18:2t10c12 in the plasma and that of 18:2t10c12 in the milk paralleled dietary supply, the level of 18:2c9t11 in the milk was similar in C and CS but still lower in S. Although the dietary concentration of CLA was highest in treatment C, the partial replacement of CLA by sunflower seeds had a similar inhibitory effect on milk fat synthesis. Comparable 18:2c9t11 levels in the milk in both CLA treatments implies that this isomer is subjected to greater biohydrogenation with increasing supply than 18:2t10c12. The fact that unprotected 18:2t10c12 escaped biohydrogenation in sufficient amounts to affect milk fat synthesis reveals opportunities to develop feeding strategies where reduced milk fat production is desirable or required by the metabolic state of the cow.

• 영재교육연구
• /
• 제24권6호
• /
• pp.1001-1024
• /
• 2014

본 연구에서는 과학영재학교에서 AP(Advanced Placement)를 경험하고 현재 대학생이 된 39명의 영재고 졸업생들과의 심층인터뷰를 통해 영재학교에서 운영 중인 AP의 운영실제 및 그 의미를 탐색하고 2015년부터 과학고로의 전면적 확대를 앞두고 있는 AP의 향후 방향성을 모색해보고자 하였다. 현재 영재학교 학생들은 기초필수와 선택과목 등으로 평균 8과목 이상의 AP 과목을 수강하고 있었다. AP 교과목의 경우, 대개는 영재학교 2학년부터 수강이 이루어지는 것이 일반적이나 PT(placement test)를 통해 1학년 때부터 AP과목을 수강하는 경우도 있었다. AP로 제공되는 선택과목들은 전공별로 차이가 있어 수학 교과에서는 비교적 많은 전공 선택 과목이 제공되고 있으나 생물이나 전산 교과등을 이수하려는 학생들에게는 상대적으로 선택의 폭이 적었다. 본 연구에 참여한 학생들에 의하면 영재학교에서의 AP는 오히려 대학에서의 수업보다 내용상 더 어려웠으며 소수인원으로 진행되는 만큼 활발한 교사-학생 상호작용과 함께 충실하게 운영되었다. 하지만 AP 과목의 이수 인정 학점인 C+의 타당성과 관련해서는 상이한 의견이 제시되었다. 연구결과 학생들은 AP로 단축된 시간을 다양한 측면으로 활용하고 있었다. AP가 0.5년에서 1.5년의 조기졸업을 위한 통로로도 활용되어지지만, 학생들은 AP로 단축된 시간을 복수전공, 부전공, 해외 교환학생 등과 같이 다양한 기회로 활용하고 있음을 알 수 있었다. 특히 남학생들의 경우 AP로 인해 단축된 시간이 있어 군복무 기간에 대해 다소 부담감을 덜 느끼는 것을 알 수 있었고, AP가 학생 개개인 관심사나 연구를 위한 시간적 여유를 제공해주기도 하였으며, 무엇보다 AP를 통해 고등학교에서 보다 진지하게 진로를 탐색하고 경험할 수 있는 유용한 시간이었다고 학생들은 지적한다. 본 연구는 과학영재학교와 과학고등학교에서 AP 과정이 성공적으로 정착하고 더욱 확대 발전하기 위해서 학교-대학 간의 보다 원활한 행정시스템 개선, AP 과목 인정 대학의 확대 등을 포함한 몇 가지 현실적 제언을 제시하고 있다.

• 한국정보처리학회:학술대회논문집
• /
• 한국정보처리학회 2013년도 추계학술발표대회
• /
• pp.230-232
• /
• 2013

본 논문에서는 실내 음영지역에서 사용되는 모바일 와이맥스의 펨토셀 기지국(WFAP; WiMAX Femtocell AP)에 대한 성능을 분석하였다. 시뮬레이션을 통해 와이맥스 펨토셀 기지국의 패킷에러율(PER), Outage, throughput을 확인하였다.