• 미생물학회지
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• 제29권2호
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• pp.111-116
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• 1991

Rhizopus nigricans produces 11.alpha.-hydroxyprogesternoe with a unidentified byproduct, which is hardly separated. Results of chromatography, IR and NMR spectroscopy identified the byproduct to be 11.alpha.-hydroxy-allopregnane-3,20-dione. R. nigricans was found to transform progesternoe into a monoform intermediate, 11.alpha.-hydroxyprogesterone, from which 11.alpha.-hydroxy-allopregnane-3,20-dione and 6.betha., 11.alpha. - dihydroxyprogesterone were formed respectively by 5.alpha.-reduction and 6.betha.-hydroxylation.

• 미생물학회지
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• 제23권4호
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• pp.297-301
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• 1985

The dependence of activities of Aspergillus phoenicis on the culture conditions in the progesterone transformation reaction was investigated. In the beginning of the reaction, $6{\beta},\;11{\alpha}-dihydroxyprogesterone$ was not produced even at high concentration of $11{\alpha}-hydroxyprogesterone$. However, large amount of the product was obtained after the complete exhaustion of progesterone. When spores of A.phoenicis replaced mycelia as enzyme source, $11{\alpha}-hydroxyprogesterone$ was produced after a considerably long indyction period, and its maximum production rate followed the exponential growth phase. The $6{\beta}-hydroxylation\;of\;11{\alpha}-hydroxyprogesterone$ continued, even after the stationary growth phase. A. phoenicis showed high enzyme activity for these reactions when the phosphate buffer solutions were used in place of the ordinary culture medium. The buffer solutions of low pH gave more yield of $11{\alpha}-hydroxyprogesterone$ than those of high pH. However, the addition of flucose to the buffer solutions did not activate the transformation reaction. The presence of progesterone seems to be necessary for the induction of enzymes for the $6{\beta}-hydroxylation\;of\;11{\alpha}-hydroxyprogesterone\;since\;6{\beta},\;11{\alpha}-dihydroxyprogesterone$ is not produced in the reaction medium containing only $11{\alpha}-hydroxyprogesterone$ as a substrate.

• 한국균학회지
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• 제13권2호
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• pp.83-87
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• 1985

A. phoenicis는 고온에서도 높은 lactose hydrolysis에 대한 효소 반응 활성을 가지고 있으나, 이 균주에 의한 progesterone의 전환 반응은 반응 온도에 매우 민감한 것으로 나타났다. 균체 내외부의 반응액 조성을 sonication 방법을 이용하여 조사함 결과 반응 초기에 균체의 내부에는 외부에 비하여 $11{\alpha}-hydroxyprogesterone$이 더 높은 농도로 존재하였으나 반응이 진행됨에 따라 균체 내부의 $11{\alpha}-hydroxyprogesterone$이 다른 생성물로 전환되어 오히려 더 낮은 농도의 $11{\alpha}-hydroxyprogesterone$이 존재하였다. A. phoenicis mycelium은 유기용매가 존재하는 반응액내에서도 새로운 액체 배지가 연속적으로 공급될 경우 물질대사를 계속 진행시킬 수 있으며, mycelium의 성장을 저해하지 않는 방법으로 고정된 cell을 이용하여 고정층 반응기에서 연속적으로 A. phoenicis-hydroxyprogesterone을 생산할 수 있다고 판단된다.