• Genomics & Informatics
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• v.18 no.1
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• pp.1.1-1.2
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• 2020

• Genomics & Informatics
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• v.21 no.1
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• pp.1.1-1.1
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• 2023

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.6
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• pp.563-569
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• 2013

This experiment was carried out to investigate the effect of liquid-pig-manure application method (Tr.1: surface appl. LPM 150%, Tr.2: surface appl. LPM 50% + LPM 50%, Tr.3: chemical fertilizer, Tr.4: soil injection LPM 150%, Tr.5: soil injection LPM 50% + surface appl. 50%, Tr.6: no fertilizer) and the economic efficiency of yield on forage crops cultivaion. In barley cultivation experiment, Tr.5 was found to be best, showing the yield of 1,462 kg $10a^{-1}$, and followed by Tr.2(1,226 kg $10a^{-1}$), Tr.3(1,226 kg $10a^{-1}$), Tr.4(1,225 kg $10a^{-1}$) and Tr.1(1,209 kg $10a^{-1}$) in order. In maize cultivation experiment, Tr.4 was found to be best, showing the yield of 2,142 kg/10a, and followed by Tr.1(2,125 kg $10a^{-1}$), Tr.3(2,024 kg $10a^{-1}$), Tr.5(2,011 kg $10a^{-1}$) and Tr.2(1,925 kg 1$10a^{-1}$) in order. The income was showing 1,274,000 ~ 1,591,000 Won $10a^{-1}$ in barley, whereas 766,000-794,000 Won 1$10a^{-1}$ in maize. There was more economical efficiency in barley cultivation than maize cultivation. Rather than the LPM surface application, LPM soil injection was more effective.

• Biomedical Science Letters
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• v.18 no.3
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• pp.201-209
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• 2012

Apoptosis is a physiological programmed cell death process. Tubercle bacilli inhibit apoptosis of alveolar macrophages and phagolysosome fusion. We investigated whether the Bcl-2 family anti-apoptotic member, Bfl-1/A1, plays an important role in the anti-apoptotic process during mycobacterial infection. PMA-treated human monocytoid THP-1 cells were infected with mycobacteria (H37Rv, BCG, and K-strain) at a multiplicity of infection (MOI) of 10 for 0, 1.5, 3, 6, 9, 12, 18, 24, 48, or 72 h. In addition, PMA-treated THP-1 cells were pretreated with specific inhibitors for 45 min before stimulation with mycobacteria at an MOI of 10 for 4 h. After the indicated time, the cells were subject to reverse transcription-polymerase chain reaction (RT-PCR) analysis, and a Bfl-1/A1-specific Western blot was performed. In PMA-differentiated THP-1 cells, the expression level of Bfl-1/A1 mRNA was increased by Mycobacterium tuberculosis (MTB) H37Rv infection. The mRNA level of Bfl-1/A1 peaked 3 h after MTB infection, then declined gradually until 9 h. However, Bfl-1/A1 mRNA induction gradually re-increased from 24 h to 72 h after MTB infection. No difference in Bfl-1/A1 expression was detected following infection with MTB H37Rv, K-strain, or M. bovis BCG. These results were not dependent on mycobacterial virulence. Moreover, mRNA levels of other anti-apoptotic molecules (Mcl-1, Bcl-2, and Bcl-xL) were not increased after MTB H37Rv or K-strain infection. These results suggest that mycobacteria induce the innate immune host defense mechanisms that utilize Bfl-1/A1 molecules at early time points, regardless of virulence.

• Environmental Mutagens and Carcinogens
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• v.23 no.2
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• pp.45-50
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• 2003

Biological activities of PAHs are not known although PAHs are considered as carcinogens. Recent industrial society has human widely exposed to PAHs (polynuclear aromatic hydrocarbons) that are comming from the incomplete combustion of organic material as wider spread environmental contaminants. Our laboratory have been studied the effect of PAHs in the human breast cancer MCF-7 cells. In this study, we examined the human breast cancer MCF-7 cells as a new system to evaluate bioactivity of PAHs. We have selected 13 PAHs to examine bioassay using CYP1A1-luciferase reporter gene expression system where CYP1A1 1.6 Kb 5flanking region DNA was cloned in front of luciferase reporter gene and this plasmid was transfected into MCF-7 cells transiently. This cells then used for the study to observe the effect of PAHs. We demonstrated that PAHs induced the CYP1A1 promoter, CYP1A1 mRNA and 7-ethoxyresolufin O-deethylase (EROD) activities in a concentration-dependant manner. None of PAHs that we have tested showed stronger stimulatory effect on CYP1 gene expression than TCDD. Benz(a)anthracene and benzo(b)fluoranthene were weak responders to CYP1A1 promoter activity stimulation, CYP1A1 mRNA and EROD induction in MCF-7 cells and these chemicals seemed to respond less either CYP1A1 mRNA or EROD than CYP1A1 promoter activity. Benzo(k)fluoranthene, chrysene, and dibenzo(a, h)anthracene showed strong response to CYP1A1 promoter activity stimulation, CYP1A1 mRNA increase and also EROD induction in MCF-7 cells. Results of dose response study suggested that two strong responding PAHs, such as benzo(k)fluoranthene and dibenzo(a, h)anthracene might be mediated through Aryl hydrocarbon receptors system in MCF-7 cells.

• The Pure and Applied Mathematics
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• v.11 no.1
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• pp.97-102
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• 2004

In this paper we establish some recurrence relations satisfied by quotient moments of upper record values from the Pareto distribution. Let ｛$X_n,n\qeq1$｝be a sequence of independent and identically distributed random variables with a common continuous distribution function(cdf) F($chi$) and probability density function(pdf) f($chi$). Let $Y_n\;=\;mas{X_1,X_2,...,X_n}$ for $ngeq1$. We say $X_{j}$ is an upper record value of {$X_{n},n\geq1$}, if $Y_{j}$＞$Y_{j-1}$,j＞1. The indices at which the upper record values occur are given by the record times ${u( n)}n,\geq1$, where u(n) = min{j｜j ＞u(n-l), $X_{j}$＞$X_{u(n-1)}$,n\qeq2$ and u(l) = 1. Suppose $X{\epsilon}PAR(\frac{1}{\beta},\frac{1}{\beta}$ then E$(\frac{{X^\tau}}_{u(m)}}{{X^{s+1}}_{u(n)})\;=\;\frac{1}{s}E$ E$(\frac{{X^\tau}}_{u(m)}{{X^s}_{u(n-1)}})$ - $\frac{(1+\betas)}{s}E(\frac{{X^\tau}_{u(m)}}{{X^s}_{u(n)}}$ and E$(\frac{{X^{\tau+1}}_{u(m)}}{{X^s}_{u(n)}})$ = $\frac{1}{(r+1)\beta}$ [E$(\frac{{X^{\tau+1}}}_u(m)}{{X^s}_{u(n-1)}})$ - E$(\frac{{X^{\tau+1}}_u(m)}}{{X^s}_{u(n-1)}})$ - (r+1)E$(\frac{{X^\tau}_{u(m)}}{{X^s}_{u(n)}})$]

• Environmental Mutagens and Carcinogens
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• v.24 no.3
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• pp.128-136
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• 2004

CYP1A1 is known to be inducible by xenobiotic compouds such as polyciclic aromatic hydrocarbons(PAHs) and 2,3,7,8-tetrachloro-dibenzo-p-dioxin(TCDD). These chemicals have been identified worldwide and can have a significant impact on the human health and well being of human and wildlife. Given these issues, the detection and quantification of these chemicals in biological, environmental and food samples is important. First, we investigated the effect of on CYP1A1 promoter activity, 7-ethoxyresorufin-O-deethylase(EROD) activity and CYP1A1 mRNA expression induced by benzo(k)fluoranthene(B(k)F) in MCF-7 cells. We found that B(k)F significantly up-regulates the level of CYP1A1 prompter activity, EROD and CYP1A1 mRNA. When cells were treated with genistein, it was not changed that EROD and CYP1A1 mRNA, compared to that of control. However, genistein inhibited the B(k)F-induced CYP1A1 promoter activity and mRNA level at high concentration. Furthermore, in this study, effects of HDAC(histone deacetvlase) inhibitors on human prostate cancer cells proliferation were examined. HC-toxin, SAHA and TSA inhibited cell proliferation in PC3 cells. A novel HDAC inhibitor, IN2001 also suppressed the growth of PC3 cells. And IN2001 and SAHA increased S phase and G2/M phase at 12 hrs treatment but cells were arrested G0/G1 phase at 45 hrs treatment. The HC-toxin treatment for 24 hrs and 48 hrs increased G0/G1 at low concentration ($0.1\mu\textrm{m}$) but increased G2/M at more than concentration of $1\mu\textrm{m}$. TSA increased G2/M phase. These findings height the possbility of developing HDAC inhibitors as potential anticancer therapeutic agents for the treatment of prostate cancer.

• Journal of the Korean Society of Food Science and Nutrition
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• v.16 no.4
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• pp.328-332
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• 1987

The aroma components analysis of raw and boiled mushroom (Lentinus edodes) by GC, GC-MS is as follows; 1) The Volatile aroma component of raw mushroom was identified as 10 Kinds, and among them, 4 Kinds of aroma component such as 1-octene-3-ol(71.05%), ethyl acetate(1.17%), 2-octenol(1.22%) and octyl alcohol(1.05%)-consists 74.47% of total aroma component. 2) meanwhile, The Volatile aroma component of boiled one was identified as 8 kinds and 5 of them 1-octene-3-ol(83.68%), ethylacetate(2.24%), 2-octenol(1.55%), octylalcohol(1.28%) and 1,2,4-trithiolane(1.91%)-consists 90.56% of total aroma component. 3) 1,2,4-trithiolane and 1,2,4,5-tetrathiane were proved to be a characteristic aroma component as sulphur compounds in Lentinus edodes.

• Journal of Bio-Environment Control
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• v.7 no.2
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• pp.116-122
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• 1998

This study was conducted to investigate growth and flowering of hydroponically-grown carnation as affected by substrate and planting date, Three substrates, coir, perlite, and coir＋perlite(1：1. v/v), and two planting dates. May 1 and September 1 were used. Plant height and stem diameter at harvesting time of cut flowers were greater for the September 1 planting than for the May 1 planting. The plants planted on May 1 produced flowers with weak stems and short stem lengths. In addition, flower weight and blossom width were gloater for the September 1 planting than for the Mar 1 planting. The planting date had no significant effect on the number of petals, The carnation planted on May 1 flowered 50 days earlier compared to those Planted on September 1. Plant height and number of petals were the greatest in the plot of coir substrate. The results indicated that for commercial production of cut carnations in a hydroponic system, planting on September 1 is better than May 1. In addition. the results confirm that coir is the superior substrate for the production of cut carnations in a hydroponic system compared to either Perlite or coir＋perlite mixture.

• Polymer(Korea)
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• v.31 no.4
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• pp.302-307
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• 2007

The resistive-type humidity sensors were prepared from the copolymers of [2-[(methacryloyloxy)ethyl]dimethyl]propylamonium bromide (MEPAB), [2-[(methacryloyloxy)ethyl]-2-hydroxyethyl]dimethylammonium bromide (MEHDAB), 2- [(methacryloyloxy)ethyl]trimethylammonium chloride (METAC), and n-butyl methylacrylate (MBA). Four component copolymers MEPAB/BMA/MEHDAB/METAC=4/4/1/1, 3/5/1/1, 2/6/1/1, 1/7/1/1 crosslinked with blocked-isocyanate on Ag/Pd electrode/alumina substrate showed a good durability at high humidities. The various electrical properties such as frequency dependency, temperature dependency, hysteresis, response time and water durability were examined. In the case of copolymer composed of MEPAB/BMA/MEHDAB/METAC=2/6/1/1, the resistance varied from $1.4\;M{\Omega}$ to $2.9\;k{\Omega}$ at $25^{\circ}C$ in the range of $30{\sim}90\;%RH$ and this copolymers showed a good linearity and low hysteresis.