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홍삼 물추출물이 Trypsin 활성에 미치는 영향 (Effect of Red Ginseng Water Extract on Trypsin Activity)

  • 이종원;김나미;도재호
    • Journal of Ginseng Research
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    • 제28권3호
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    • pp.127-131
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    • 2004
  • 홍삼 물 추출물이 효소활성에 미치는 영향을 조사하기 위하여 먼저 홍삼분말에 methyl alcohol을 가하여 지용성 성분과 사포닌 성분을 추출하여 제거하였다. 그 잔사에 정제수를 가하여 추출한 뒤 추출물을 제조하였고, 투석막을 이용하여 분자량 크기별로 분획한 뒤 trypsin의 활성에 미치는 영향을 조사하였다. 몇 가지 분획 중에서 분자량 1,000∼12,000 사이의 분획이 trypsin 활성을 가장 강하게 증가시켰다. 분자량 1,000∼12,000 사이의 분획을 여러 가지 농도별로 첨가하여 trypsin 활성에 미치는 영향을 조사한 결과 2.9${\times}$$10^{-3}$%에서 15% 정도 촉진되었으며, 농도가 높아질수록 trypsin 활성도 증가하여 9${\times}$$10^{-2}$%에서 최고의 활성을 보이다가 그 이상의 농도에서는 감소하였다. Trypsin의 casein 분해에 있어서 물 추출물은 Km 값을 낮게하고 Vmax 값은 증가기켰다. 물 추출물을 부분 정제하여 몇 가지 특성을 조사한 겨로가 ninhydrin, DNS 및 Folin시약에 양성반응을 나타내는 것으로 보아 함질소 화합물이라고 판단된다.

우리나라 지역별 기온변화 특성 (A Study on the Air Temperature Changes and Regional Characteristics in South Korea)

  • 김태룡
    • 통합자연과학논문집
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    • 제2권2호
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    • pp.131-167
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    • 2009
  • Global warming is regarded as one of the most critical issues that should be taken care of by the entire global community as it threatens the survival of mankind. South Korea, in particular, undergoes faster warming than the average rate of global warming. South Korea has revealed various warming rates and trends being surrounded by sea on three sides and having complex terrains dominated by mountains. The rates vary according to regions and their urbanization and industrialization. Differences also derive from seasons and weather elements. Changes to the highest, mean, and lowest temperature are also different according to the characteristics of regions and observatories, which is more apparent where the force of artificial weather applies. In an urban area, temperature gaps tend to decrease as the lowest temperature rises more than the highest temperature. Meanwhile, temperature gaps grow further in a coastal or country region where the force of artificial weather is small and the force of natural weather prevails. In this study, the investigator analyzed the changes to the weather elements of 11 observation spots that had gone through no changes in terms of observation environment since 1961, were consecutively observed, and had the quality of their observation data monitored on an ongoing basis. Using the results, I tried to identify natural and artificial causes affecting certain spots. Located on the east coast of the Asian Continent, South Korea sees weather changing very dynamically. Having huge influences on our weather, China has achieved very rapid industrialization for the last 30 years and produced more and more greenhouse gases and air pollution due to large-size development projects. All those phenomena affect our weather system in significant ways. Global warming continues due to various reasons with regional change differences. Thus the analysis results of the study will hopefully serve as basic data of weather statistics with which to set up countermeasures against climate changes.

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사암침법(舍岩鍼法)의 화열(火熱)에 대한 연구 (Study on the Principles of Prescriptions of Fire and Heat of Saam Acupuncture)

  • 사공희찬;김병수;강정수
    • Journal of Acupuncture Research
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    • 제22권4호
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    • pp.131-141
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    • 2005
  • Objectives : Saam acupuncture has evident principles of prescription based on acupuncture tonification and purgation of Nan-gyeong but many prescriptions of Saam acupuncture are not typical patterns. This paper studies principles of prescriptions of the king fire and the premier fire. Methods : Through investigation of many medical documents concerning king fire and the premier fire, the author explained of physiological and pathological principle of king and the premier fire, also explained of interacting mechanism through five element. And The theoretical authorities of analysis are Nan-gyeong, works of Joo dan-gye and Li dong-won. Results & Conclusion : 1. The sufficiency and the control of the water is the key to treat the disease by fire. Considering the attack and the treatment of disease, heart and liver give rise to fire in its early stage and kidney and lung are the key to treatment. 2. Consequently the pathology of the disease from fire is corresponding to the theory- Yang Sufficient, Yin Deficient- of Joo dan-gye. It seems that the theory of Joo dan-gye influenced on the medical theory of Saam Acupuncture. 3. On that account I believe that the study of medical theories of the Geum and Won Dynasty that various medical theories had appeared is very available for Saam Acupuncture. At present global warming is accelerating because environmental pollution has been increasing. Therefore the damage of fire to human body will be more and more severe and extensive. Because in the future medical plants will not be free from environmental pollution, acupuncture will be very confidential treatment.

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초기 영구 전치 파절시의 치아 재부착술의 임상 증례 (CLINICAL EFFORTS FOR TECHNICAL IMPROVEMENT IN TOOTH FRAGMENTATION)

  • 최성철;이긍호;최영철
    • 대한소아치과학회지
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    • 제29권1호
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    • pp.125-131
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    • 2002
  • 본 증례는 경희대학교 치과대학병원 소아치과학교실에 상악 중절치의 치수노출을 동반한 외상을 주소로 내원한 아동의 치험례이다. 증례 1은 미성숙 치근을 가지는 만 8세의 남아로 치아의 치수 노출에 따른 수산화칼슘 치수절단술을 이용한 치근단 형성술을 시행한 후 치아 재부착술을 시행한 증례이며, 증례 2는 점상의 치수노출을 보이는 미성숙 영구 중절치를 직접 치수 복조 추 석회화 층을 형성하는 기간동안 레진을 이용하여 유지한 후 치아 재부착술을 시행하였고 증례 3은 치은 연하로 치경부 파절을 보이는 만 12세의 환아로 신경치료 후 골 삭제술과 골 성형술을 동반한 판막술을 시행하여 carbon fiber post를 이용한 치아 재부착술을 시행한 증례이다 이상의 증례를 통하여 치아 재부착술을 성공적으로 시행하였으며 심미적으로 양호한 결과를 얻었기에 보고하는 바이다.

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막생물반응기(MBR)에 의한 염료용액의 처리연구 (Decolorization of dye solution using membrane bioreactor (MBR) by Trametes versicolor)

  • 이유리;김현;박철환;이병환;김상
    • 청정기술
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    • 제10권3호
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    • pp.131-137
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    • 2004
  • 오래 전부터 생물학적 폐수처리 방법에 대한 다양한 연구들이 꾸준히 진행되고 있으나, 전통적인 염료 염색 폐수처리 방법들은 다소 비효율적이다. 최근 들어 이를 극복하기 위하여 특정 미생물이나 효소를 이용한 연구들이 활발히 진행되고 있다. 본 연구에서는 백색부후균이 생산하는 리그닌 효소에 의한 생물처리 방법으로, 아조계(azo) 염료인 Reactive black 5를 적용하였으며, 다양한 농도에 따른 분해경향을 확인하였다. 미생물 재이용과 장기간 운전, 효율적인 유기물 제거의 가능성을 확인하기 위해 i)생물학적 처리와 ii)분리막 처리공정과 iii)이 두 가지를 결합한 공정의 처리 효율을 비교하였다. 결합공정을 사용하였을 때 색도제거율이 100%였으며, 총유기물(TOC) 제거율은 70% 이상으로 각 단위공정만으로 처리하였을 때보다 더 효율적인 결과를 보였다. 또한, 생물반응에서 배양말기까지 유지된 효소활성(40 U/ml laccase and 300-600 U/ml MnP)을 통해 미생물 재이용의 가능성을 확인하였다.

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도시(都市) BUS 운행저해요인(運行沮害要因)에 관한 연구(硏究) (A Study on the Primary Factors Delaying City-BUS Service)

  • 김대웅;유영근
    • 대한토목학회논문집
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    • 제7권2호
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    • pp.131-139
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    • 1987
  • 본(本) 연구(硏究)에서는 대부분(大部分)의 도시(都市)에서 대중교통수단(大衆交通手段)으로써 주요(主要)한 역할(役割)을 담당(擔當)하고 있는 버스 교통(交通)이 운행시(運行詩) 겪게되는 저해요인(沮害要因)들을 규명(糾明)하고 구간평균(區間平均) 주행속도(走行速度) 모형(模型)을 개발(開發)하는데 연구(硏究)의 목적(目的)을 두었다. 2 대(臺)의 video camera 로 속도계(速度計)와 전방(前方)의 교통상황(交通狀況)을 동시(同時)에 촬영하여 시간(時間)-속도선도(速度線圖)를 작성(作成)한 후, 가로교통규제(街路交通規制)의 상한속도선(上限速度線)에 미달(未達)되는 구간(區間)을 저해구간(沮害區間)으로 보고 이 구간(區間)의 저해요인(沮害要因)을 monitor를 통하여 분석(分析)하였다. 이상(以上)에서 파악(把握)된 저해요인(沮害要因)를 수량화(數量化) 이론(理論)(1류(類))에 의하여 통계적(統計的)으로 규명(糾明)하고 이를 설명변수(說明變數)로 도입(導入)하여 단계적(段階的) 회귀분석(回歸分析) 방법(方法)으로 버스 구간평균(區間平均) 주행속도(走行速度) 모형(模型)을 구축(構築)하였다.

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초동발췌를 위한 탄성파 신호분석연구 (A Study in Seismic Signal Analysis for the First Arrival Picking)

  • 이두성
    • 지구물리와물리탐사
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    • 제10권2호
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    • pp.131-137
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    • 2007
  • 초동발췌 방법과 이와 관련된 오차문제를 고찰하고 자료처리 전산화 측면에서 초동발췌 및 오차계산의 실용적 알고리즘을 제안하였다. 제안한 초동발췌 방법은 2단계로 구성된다. 1) 초동신호 중 트레이스 간 연속성이 양호한 최초의 피크(peak) 또는 트라프(trough)를 발췌한다. 2) 발췌시점 전방 일정구간의 기록을 직선으로 근사화 하고 이 직선의 시간 절편을 초동주시로 한다. 근사화 구간의 길이는 대략 초동 웨이브렛 폭의 1/4보다 다소 작게 설정한다. 초동발췌에 내재된 오차의 정량적인 척도는 기록의 특정시점에 초동의 도착 여부를 판단하는 데 필요한 기록의 길이로 정의한다. 기록의 길이를 나타내는 공식은 신호의 주파수대역과 신호대잡음비의 함수로 표시된다. 3개의 공대공 탄성파기록의 초동을 수동 및 제안한 방법으로 발췌하였고 각 트레이스의 오차한계를 계산하였다. 실험결과 제안한 근사직선 시간절편법의 우수한 성능과 발췌된 초동평가에 있어서 발췌오차의 유용성을 확인하였다.

PHEOLOGICAL PROPERTIES OF OIL/WATER EMULSION AND OIL/LIQUID CRYSTAL/WATER SYSTEMS AND THEIR CONSUMER PERCEPTION IN HAIR CARE PRODUCTS

  • Kim, Chongyoup;Jongeoun Hong;Kim, Suhyun;Hakhee Kang
    • 대한화장품학회:학술대회논문집
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    • 대한화장품학회 1999년도 IFSCC . ASCS 학술대회 발표 논문
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    • pp.123-131
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    • 1999
  • Liquid crystal known as a rheological barrier to coaleseence of oil dorplets. increases. emulsion stability, water-holding capacity and promotes active material penetration to skin. Some investigation for its rheological characteristics have been reported but its relations to consumer perception have been rarely published. In this study oil/water emulsion and oil/liquid crystal/water systems were manufactured using the same composition of Behenyltrimethylammonium chloride/Cetostearyl alcohol/Lanolin oil. and rheological properties of each system were investigated with Cone and Plate rheometer. The formation of liquid crystalline phase was observed with polarized microscope and Differential Scanning Calorimeter. Continuous shear experiment, creep yield and water holding capacity were measured for oil/water and oil/liquid crystal/water systems. The results were compared with sensory evaluations. Oil/liquid crystal/water system showed higher viscosity at the same shear rate, higher viscoelasticity and higher yield stress than oil/water system. These properties were expected to show good spreadability and excellent richness without waxiness I hair care products of creme type. This expectation was consistent with the results of sensory experiments. Water-holding capacity was evaluated by measuring residual water of specimens at specific temperature and relative humidity, Oil/liquid crystal/water system was proved to have ability to hold water in comparison with oil/water system. The results indicated that oil/liquid crystal/water system was of benefit to rheological properties creme type hair care products.

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오령산(五?散)이 CCl4 투여(投與)로 인한 흰쥐의 손상간(損傷肝)에 미치는 영향(影響) (Effect of the Oryungsan on the injured liver in CCl4-treated rats)

  • 권오성;최홍식;이준희;강윤호
    • 동국한의학연구소논문집
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    • 제5권
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    • pp.131-147
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    • 1996
  • This study was investigated to define effect of pretreatment of Oryungsan on hepatotoxicity induced by $CCl_4$ in rat. The experimental anmals were divided into normal group, control group, sample group, and sample group were divided sample1, sample2, sample3. Control group were injected diluted carbon tetrachloride and olive oil, $1m{\ell}/kg$ to intraperitoneal after 8th day. Sample group were injected Oryungsan water extracts, $0.33m{\ell}/kg/day$ to intraperitoneal of sample group1, $0.66m{\ell}/kg$ day to sample group2, $1.32m{\ell}/kg/day$ to sample group 3 each other for 7day, and injected the dosage of $CCl_4$ was $1.0m{\ell}/kg$ diluted 1 : 1 with olive oil after 8th day. The following results were made by observation GOT, GPT, Albumin, Globulin, Bile acid, hepatic tissue. 1. The serum GOT and GPT level of sample group were decreased. Sample group 1 were decreased significantly. 2. The serum A/G ratio of sample group was increased as compared with normaI group. 3. The serum bile acid of sample group was decreased significantly. 4. The destruction of hepatic tissue were repaired as compared with control group. These results demonstrated that oryungsan can be attributed to recovery from hepatotoxicity.

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Floristic Study of Gyodongdo Island in Ganghwa-gun, Korea

  • Kim, Jung-Hyun;Kim, Sun-Yu;Lee, Byoung Yoon;Yoon, Chang-Young
    • 한국환경생태학회지
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    • 제29권2호
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    • pp.105-131
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    • 2015
  • This study was carried out to investigate the flora of Gyodongdo island (Ganghwa-gun). The vascular plants from 11 field surveys were revealed to belong to a total of 629 taxa; 118 families, 364 genera, 561 species, 5 subspecies, 53 varieties, 7 forms and 3 hybrids. 184 taxa were the first records from this region. The plants in Gyodongdo island are composed of the deciduous broad-leaved and conifer-mixed forests which are the common ones in the middle part of the Korean Peninsula. Five taxa of Korean endemic plants such as Clematis brachyura Maxim., Viola seoulensis Nakai, Populus ${\times}$ tomentiglandulosa T. B. Lee, Forsythia koreana (Rehder) Nakai and Hemerocallis hakuunensis Nakai were collected. Endangered wild plants designated by the law called 'Protection Law for Endangered wild fauna and flora' were one taxon. The red list plants according to IUCN valuation basis were examined for 13 taxa; endangered (EN) species of Prunus yedoensis Matsum., Vulnerable (VU) species of both Utricularia pilosa (Makino) Makino and Iris ruthenica var. nana Maxim., Near Threatened (NT) species of Senecio argunensis Turcz., Least Concern (LC) species of Platycladus orientalis (L.) Franco, Potentilla discolor Bunge, Limnophila sessiliflora (Vahl) Blume, Acorus calamus L., Phacelurus latifolius (Steud.) Ohwi, Pseudoraphis ukishiba Ohwi, Belamcanda chinensis (L.) DC., and Not Evaluate (NE) species of both Astragalus sikokianus Nakai and Potamogeton oxyphyllus Miq. The floristic regional indicator plants found in this area were a total of 47 taxa comprising three taxa of grade V, four taxa of grade IV, nine taxa of grade III, 10 taxa of grade II, and 21 taxa of grade I. The naturalized plants were identified as 62 taxa and the percentage of naturalized index (NI) was 9.9 % and the percentage of urbanization index (UI) was 19.3 %, respectively. Furthermore, hemicryptophytes (28 %), therophytes (26 %), hydrophytes (13 %) and geophyte (12 %) showed high proportional ratio in life form spectrum.