• BMB Reports
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• v.51 no.7
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• pp.356-361
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• 2018

Actin-binding LIM protein 1 (ABLIM1), a member of the LIM-domain protein family, mediates interactions between actin filaments and cytoplasmic targets. However, the role of ABLIM1 in osteoclast and bone metabolism has not been reported. In the present study, we investigated the role of ABLIM1 in the receptor activator of $NF-{\kappa}B$ ligand (RANKL)-mediated osteoclastogenesis. ABLIM1 expression was induced by RANKL treatment and knockdown of ABLIM1 by retrovirus infection containing Ablim1-specific short hairpin RNA (shAblim1) decreased mature osteoclast formation and bone resorption activity in a RANKL-dose dependent manner. Coincident with the downregulated expression of osteoclast differentiation marker genes, the expression levels of c-Fos and the nuclear factor of activated T-cells cytoplasmic 1 (NFATc1), critical transcription factors of osteoclastogenesis, were also decreased in shAblim1-infected osteoclasts during RANKL-mediated osteoclast differentiation. In addition, the motility of preosteoclast was reduced by ABLIM1 knockdown via modulation of the phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt/Rac1 signaling pathway, suggesting another regulatory mechanism of ABLIM1 in osteoclast formation. These data demonstrated that ABLIM1 is a positive regulator of RANKL-mediated osteoclast formation via the modulation of the differentiation and PI3K/Akt/Rac1-dependent motility.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.07b
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• pp.938-941
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• 2003

It is well known that the state of existence of molecules on the surface of water changes during compression of the molecules. Electric methods, such as measurement of the surface potential or displacement current are also useful for investigating dynamic changes of molecular state on the water surface during compression and Transformation of molecular film occurs only usually in air-water interface, 2 dimensions domain's growth and crash are achieved. Organic thin film that consist of growth of domain can understand correct special quality of accumulation film supplying information about fine structure and properties of matter of device observing information and so on that is surface forward player and optic enemy using AFM one of SPM application by nano electronics. In this paper Langmuir (L) that is one of basis technology to manufacture of organic matter device using biology material PBDG that is kind of polypeptide that have biology adaptedness. The Experiment method used ${\pi}-A$ isotherm and BAM(Brewster Angle Microscopy), using the BAM, we can to the molecular orientation of monolayer on the water surface and directly see the morphology of the films on water subphase as well as that of the films.

• The Korean Journal of Pharmacology
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• v.32 no.3
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• pp.311-321
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• 1996

Both $M_1$ and $M_2$ muscarinic receptors contain a triplet of amino acid residues consisting of leucine (L), tyrosine (Y) and threonine (T) at C-terminus ends of the second putative transmembrane domains. This triplet is repeated as LYT-LYT in $M_2$ receptors at the interface between the second transmembrane domain and the first extracellular loop. Interestingly, however, it is repeated in a transposed fashion (LYT-TYL) in the sequence of $M_1$ receptors. In this work, we employed site-directed mutagenesis to investigate the possible significance of this unique sequence diversity for determining the distinct differential cellular function at the two receptor subtypes. Mutation of the LYTTYL sequence of $M_1$ receptors to the corresponding $M_2$ receptor LYTLYT sequence did not result in a significant change in the binding affinity of the agonist carbachol. The reverse mutation at the $M_2$ receptor also did not modify agonist affinity. Surprisingly, the LYTLYT $M_1$ receptor mutant demonstrated markedly enhanced coupling to activation of phospholipase C without a change in its coupling to increased cyclic AMP formation. There was also an enhanced receptor sensitivity in transducing elevation of intracellular $Ca^{2+}$. On the other hand, the reverse $LYTLYT{\rightarrow}LYTTYL$ mutation in the $M_2$ receptor did not alter its coupling to inhibition of adenylate cyclase, but slightly enhanced its coupling to stimulation of phosphoinositide (PI) hydrolysis. Our data suggest that the LYTTYL/LYTLYT sequence differences between $M_1$ and $M_2$ muscarinic receptors are not important for specifying ligand binding and coupling of various subtypes of muscarinic receptors to different cellular signaling pathways although they might play a role in the modulation of muscarinic reseptor coupling to PI hydrolysis.

• Journal of Electrical Engineering and Technology
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• v.12 no.1
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• pp.236-248
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• 2017

A direct stator flux vector control scheme in discrete-time domain is proposed in this paper for the interior permanent magnet synchronous motor (IPMSM) drive to remove the proportional-integral (PI) controller from the direct torque control (DTC) scheme applied to IPMSM and to obtain faster dynamic response and lower torque ripple output. The output of speed outer loop is used as the desired torque angle instead of the desired torque in the proposed scheme. The desired stator flux vector in dq coordinate is calculated with a given amplitude. The state-space equations in discrete-time for IPMSM are established, the actual stator flux vector is estimated in deadbeat manner by a full-order state observer, and then the closed-loop control is achieved by the pole placement. The stator flux error vector is utilized to calculate the reference stator voltage vector. Extracting the angle position and amplitude from the estimated stator flux vector and estimating the output torque are eliminated for the direct feedback control of the stator flux vector. The proposed scheme is comparatively investigated with a PI-SVM DTC scheme by experiment results. Experimental results show the feasibility and advantages of the proposed control scheme.

• YAKHAK HOEJI
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• v.44 no.1
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• pp.52-59
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• 2000

An exceptionally conserved sequence that is shared among most G protein-coupled neurotransmitter receptors is an aspartate-arginine-tyrosine triplet that is located at the second cytoplasmic domain. Using the ml subtype of muscarinic acetylcholine receptors as an example, a point mutation of the arginine residue at position 123 into asparagine was induced. This mutation resulted in a complete blockade of the carbachol-induced increases of PI hydrolysis and intracellular $Ca^2$$^{+}$ level, in spite of the expression of the wild-type and mutant receptors at similar concentrations in Chinese hamster ovary cells. In marked contrast, the muscarinic agonist carbachol induced concentration-dependent enhancement of the activity of NO synthase at mutant ml receptors although the enhancement was significantly smaller than at wild-type ml receptors. These data suggest that this highly conserved arginine residue plays an important role in coupling of muscarinic receptors to the second messenger systems and the presence of alternate mechanisms of activation of neuronal NO synthase which might be operative in the absence of large changes in the concentration of cellular $Ca^{2+}$.2+/.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.14 no.1
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• pp.38-46
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• 2000

This study have established proper mathematical equivalent model of Brushless DC (BLDC) motor and estimated the motor parameter by means of the back-emf measurement as being the step input to the controlled target BLDC motor. And the validity of proposed estimation method is confirmed by the test result of step response. As well, we have designed the reasonable digital controller as a consequence of the root locus method which is obtained from the open-loop transfer function of BLDC motor with hall sensor, and the determination of control gain for variable speed control. Here, revised Ziegler-Nichols tuning method is applied for the proper digital gain establishment, and the system stability is verified by the frequency domain analysis with Bode-plot and experimentation.

• BMB Reports
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• v.32 no.2
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• pp.196-202
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• 1999

The existence of the Ras-independent signal transduction pathway of insulin leading to DNA synthesis was investigated in Rat-1 fibroblasts overexpressing human insulin receptor (HIRc-B) using the single-cell microinjection technique. Microinjection of a dominant-negative mutant $Ras^{N17}$ protein into quiescent HIRc-B cells inhibited the DNA synthesis stimulated by insulin. Microinjection of oncogenic H-$Ras^{V12}$ protein ($H-Ras^{V12}$) (0.1 mg/ml) induced DNA synthesis by 35%, whereas that of control-injected IgG was induced by 20%. When the marginal amount of oncogenic H-$Ras^{V12}$ protein was coinjected with a dominant-negative mutant of the H-Ras protein ($Ras^{N17}$), DNA synthesis was 35% and 74% in the absence and presence of insulin, respectively. This full recovery of DNA synthesis by insulin suggests the existence of the Ras-independent pathway. The same recovery was observed in the cells coinjected with either H-$Ras^{V12}$ plus H-$Ras^{N17}$ plus SH2 domain of the p85 subunit of PI3-kinase ($p85^{SH2-N}$) or H-$Ras^{V12}$ plus H-$Ras^{N17}$ plus interfering anti-Shc antibody. When co-injected with a dominant-negative H-$Ras^{N17}$, the DNA synthesis induced by the Ras-independent pathway was blocked. These results indicate that the Ras-independent pathway of insulin leading to DNA synthesis exists, bypassing the p85 of PI3-kinase and Shc protein, and requires Rac1 protein.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.65 no.10
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• pp.1648-1654
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• 2016

This paper analyzes complex vector current control for the enhanced cross-coupling compensation in accordance with parameter variation in grid-connected inverter system, and verifies through simulation and experiment. Complex vector current control is performed in the synchronous reference frame through d-q transformation. It generates cross-coupling components with rotating nominal angular frequency. In general, cross-coupling elements are compensated by decoupling terms added to output of conventional decoupling PI controller. But, it is impossible to compensate them perfectly which transient response is especially deteriorated such as large overshoot and slow tracking, when variation of grid impedance or measurement error occurs. However, complex vector current control can improve stability and response characteristic of current control regardless of the situation as before. Decoupling controller and complex vector controller are represented through complex forms, and these controllers are analyzed by using frequency response in s-domain, respectively. It is verified that complex vector controller has more superior response characteristic than decoupling controller through MATALB, PSIM and experimental in 5kW grid-connected inverter when L filter parameter is varied from 1.1mH to increase double, 2.2mH.

• 제어로봇시스템학회:학술대회논문집
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• 2005.06a
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• pp.498-501
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• 2005

This paper deals with the design and evaluation of the robust controller for a synchronous generator excitation system to improve the steady state and transient stability. The nonlinear characteristics of the system is treated as model uncertainties, and then the robust control techniques are introduced into the power system stability design to take into account these uncertainties at the controller design stage. The performance of the designed controller is examined by extensive non-linear time domain simulation. It is shown that the performance of the robust controller is superior to that of the conventional PI controller. This paper also proposes an improved digital exciter control system for a synchronized generator using a digitally designed controller with database. Results show that the proposed control system manifests excellent control performance compared to existing control systems. It has also been confirmed that it is easy for the proposed control system to implement digital control.

• International Journal of Fuzzy Logic and Intelligent Systems
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• v.5 no.1
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• pp.83-87
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• 2005

This paper deals with the design and evaluation of the robust controller for a synchronous generator excitation system to improve the steady state and transient stability. The nonlinear characteristics of the system is treated as model uncertainties, and then the robust control techniques are introduced into the power system stability design to take into account these uncertainties at the controller design stage. The performance of the designed controller is examined by extensive non-linear time domain simulation. It is shown that the performance of the robust controller is superior to that of the conventional PI controller. This paper also proposes an improved digital exciter control system for a synchronized generator using a digitally designed controller with database. Results show that the proposed control system manifests excellent control performance compared to existing control systems. It has also been confirmed that it is easy for the proposed control system to implement digital control.