• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.482-483
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• 2011

Graphene is a good candidate for the future nano-electronic materials because it has excellent conductivity, mobility, transparency, flexibility and others. Until now, most graphene researches are focused on the nano electronic device applications, however, biological application of graphene has been relatively less reported. We have fabricated a deoxyribonucleic acid (DNA) conjugated graphene field-effect transistor (FET) and measured the electrical transport characteristics. We have used graphene sheets grown on Ni substrates by chemical vapour deposition. The Raman spectra of graphene sheets indicate high quality and only a few number of layers. The synthesized graphene is transferred on top of the substrate with pre-patterned electrodes by the floating-and-scooping method [1]. Then we applied adhesive tapes on the surface of the graphene to define graphene flakes of a few micron sizes near the electrodes. The current-voltage characteristic of the graphene layer before stripping shows linear zero gate bias conductance and no gate operation. After stripping, the zero gate bias conductance of the device is reduced and clear gate operation is observed. The change of FET characteristics before and after stripping is due to the formation of a micron size graphene flake. After combined with 30 base pairs single-stranded poly(dT) DNA molecules, the conductance and gate operation of the graphene flake FETs become slightly smaller than that of the pristine ones. It is considered that DNA is to be stably binding to the graphene layer due to the ${\pi}-{\pi}$ stacking interaction between nucleic bases and the surface of graphene. And this binding can modulate the electrical transport properties of graphene FETs. We also calculate the field-effect mobility of pristine and DNA conjugated graphene FET devices.

• The Korean Journal of Physiology and Pharmacology
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• v.4 no.6
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• pp.487-496
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• 2000

Insulin stimulates glucose transport in muscle and fat cells by promoting the translocation of glucose transporter (GLUT4) to the cell surface. Phosphatidylinositide 3-kinase (PI3-kinase) has been implicated in this process. However, the involvement of protein kinase B (PKB)/Akt and $PKC-{\zeta}$, those are known as the downstream target of PI3-kinase in regulation of GLUT4 translocation, is not known yet. An interesting possibility is that these protein kinases phosphorylate GLUT4 directly in this process. In the present study, $PKB-{\alpha}$ and $PKC-{\zeta}$ were added exogenously to GLUT4-containing vesicles purified from low density microsome (LDM) of the rat adipocytes by immunoadsorption and immunoprecipitation for direct phosphorylation of GLUT4. Interestingly GLUT4 was phosphorylated by $PKC-{\zeta}$ and its phosphorylation was increased in insulin stimulated state but GLUT4 was not phosphorylated by $PKB-{\alpha}.$ However, the GST-fusion proteins, GLUT4 C-terminal cytoplasmic domain (GLUT4C) and the entire major GLUT4 cytoplasmic domain corresponding to N-terminus, central loop and C-terminus in tandem (GLUT4NLC) were phosphorylated by both $PKB-{\alpha}$ and $PKC-{\zeta}.$ The immunoblots of $PKC-{\zeta}$ and $PKB-{\alpha}$ antibodies with GLUT4-containing vesicles preparation showed that $PKC-{\zeta}$ was co-localized with the vesicles but not $PKB-{\alpha}.$ From the above results, it is clear that $PKC-{\zeta}$ interacts with GLUT4-containing vesicles and it phosphorylates GLUT4 protein directly but $PKB-{\alpha}$ does not interact with GLUT4, suggesting that insulin-elicited signals that pass through PI3-kinase subsequently diverge into two independent pathways, an Akt pathway and a $PKC-{\zeta}$ pathway, and that later pathway contributes, at least in part, insulin stimulation of GLUT4 translocation in adipocytes via a direct GLUT4 phosphorylation.

• Journal of the Korean Ceramic Society
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• v.29 no.6
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• pp.459-462
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• 1992

It has been reported that the size and characteristics of ferroelectric domains which have an essential role on the ferroelectric properties depend on the grain size of ferrolectric ceramics. Therefore understanding the change of domain characteristics with grain size is so important to know the dependence of dielectric constant, dielectric loss and aging on the grain size. In this research, the equilibrim domain with is calculated as d={{{{ SQRT { { 64 pi sigma a} over {3C11Ss2 } } }}. This calculated value is nearly same to the measured value of BaTiO3 and Pb(Zr0.4Ti0.6)O3 ceramics 90$^{\circ}$domain wall width. The calculated 90$^{\circ}$domain wall enerygy in Pb(Zr0.4Ti0.6)O3 which is obtained through the model is approximately 2$\times$10-2J/$m^2$.

• Molecules and Cells
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• v.20 no.2
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• pp.241-246
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• 2005

PDK-1 activates PI3-kinase/Akt signaling and regulates fundamental cellular functions, such as growth and survival. NF-${\kappa}B$ is involved in the induction of a variety of cellular genes affecting immunity, inflammation and the resistance to apoptosis induced by some anti-cancer drugs. Even though the crucial involvement of the PI3-kinase/Akt pathway in the anti-apoptotic activation of NF-${\kappa}B$ is well known, the exact role of PDK-1 as well as PI3-kinase/Akt in NF-vactivation is not understood. Here we demonstrate that PDK-1 plays a pivotal role in transcriptional activation of NF-${\kappa}B$ by dissociating the transcriptional co-repressor HDAC1 from the p65 subunit of NF-${\kappa}B$. The association of CBP with p65 was not directly modulated by PDK-1 or by PI3-kinase. Etoposide activated NF-${\kappa}B$ through PI3-kinase/Akt, and the transcription activation domain (TAD) of p65 was further activated by wild-type PDK-1. Overexpression of a dominant negative PDK-1 mutant decreased etoposide-induced NF-${\kappa}B$ transcription and further down-regulated the ectopic HDAC1-mediated decrease in NF-${\kappa}B$ transcriptional activity. Thus activation of PDK-1 relieves the HDAC1-mediated repression of NF-${\kappa}B$ that may be related to basal as well as activated transcription by NF-${\kappa}B$. This effect may also explain the role of the PI3-kinase/PDK-1 pathway in the anti-apoptotic function of NF-${\kappa}B$ associated with the chemoresistance of cancer cells.

• 한국정보디스플레이학회:학술대회논문집
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• 2003.07a
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• pp.551-554
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• 2003

A novel geometric solution for uniform bend transition in a pi cell has proposed. In order to obtain the uniform bend transition, we applied hybrid domain structure to the edge of a LC cell by using ion-beam alignment method. As a result, we confirmed that the uniform bend transition has arisen from the edge to the center. From the proposed structure, we expect that the reliable and uniform bend transition can be achieved in the pi cell.

• Proceedings of the KIEE Conference
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• 2004.04a
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• pp.147-149
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• 2004

This paper presents a new velocity estimation strategy of a non-salient permanent magnet synchronous motor(PMSM) drive without high frequency signal injection or special PWM pattern. This approach is based on the d-axis current regulator output voltage of the drive system which has the information of rotor position error. The rotor velocity can be estimated through a rotor position tracking PI controller that controls the position error to zero. For zero and low speed operation, the PI gains of rotor position tracking controller have a variable structure. The PI tuning formulas are derived by analyzing this control system using the frequency domain specifications such as phase margin and bandwidth assignment.

• The Transactions of the Korean Institute of Power Electronics
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• v.22 no.3
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• pp.263-269
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• 2017

This paper proposes a novel circulating current control method for an MMC-HVDC system based on Vector PI control. The method can suppress second-order harmonics of the circulating currents under balanced and unbalanced grid conditions. The proposed method is robust to grid frequency variation. The effectiveness of the proposed method is verified through frequency response and time domain simulation.

• Journal of Advanced Navigation Technology
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• v.17 no.6
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• pp.693-699
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• 2013

In this paper, ${\pi}/4$-DQPSK modem according to ITU-R M.1842-1 recommendation Annex1 is investigated and simulated. At first, standardization and technical trend of VHF maritime mobile communication are introduced. The ${\pi}/4$-DQPSK modem defined in the Annex1 should support 28.8Kbps bit transmission rate within 25KHz frequency bandwidth. We describe the system model and simulation process of ${\pi}/4$-DQPSK modem transmitter and receiver design with RRC(Root Raised Cosine) transmitter and receiver filter. Then we suggest various graphical simulation results(time domain signals, constellation, power spectral density according to roll-off factor, eye diagram), and show simulated BER performance of the modem. From the simulation results, it is shown that roll-off factor of RRC filter affects to BER performance according to SNR and the designed simulation model meets the spectrum mask requirement suggested in ITU-R M.1842-1 recommendation.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.25 no.7B
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• pp.1282-1291
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• 2000

기존의 LQ-서보형 PI 설계방법은 보드선도 상에서 저주파와 고주파 영역에서의 루프전단함수의 특이값 일치에 기이한 설계상의 문제를 가지고 있다. 이러한 점을 해결하기 위해 역 최적제어와 볼록형 최적화기법에 기초한 새로운 설계 방법을 제안하고자 한다.

• Proceedings of the Korean Magnestics Society Conference
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• 2009.05a
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• pp.22-22
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• 2009