• BMB Reports
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• 제40권6호
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• pp.845-852
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• 2007

The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 ($\underline{Ph}alaenopsis$ $\underline{PI}$ STILLATA # $\underline{15}$), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.

• 대한의생명과학회지
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• 제10권4호
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• pp.375-383
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• 2004

It has been reported that osteoporosis induced by the deficiency of estrogens in menopause is associated with the unbalance of Ca/sup 2+/ and Pi levels. Proximal tubule is very important organ to regualte Ca/sup 2+/ and Pi level in the body. However, the effect of estrogens on Ca/sup 2+/ and Pi regulation was not elucidated. Thus, we examined the effect of 17-β estradiol (E₂) on Ca/sup 2+/ and Pi uptake in the primary cultured rabbit renal proxiaml tubule cells. In the present study, E₂(> 10/sup -9/M) decreases Ca/sup 2+/uptake and stimulates Pi uptake over 3 days. E₂-induced decrease of Ca/sup 2+/ uptake and stimulation of Pi uptake were blocked by actinomycin D (a gene transcription inhibitor), cycloheximide (a protein synthesis inhibitor). tamoxifen, and progesterone (estrogen receptor antagonists). E₂-induced decrease of Ca/sup 2+/ uptake and stimulation of Pi uptake were blocked by SQ22536 (an adenylate cyclase inhibitor), Rp-cAMP (a cAMP antagonist), and PKI (a protein kinase A inhibitor). Indeed, E₂ increased cAMP formation. In addition, E₂-induced decrease of Ca/sup 2+/ uptake and stimulation of Pi uptake were blocked by staurosporine, H-7, and bisindolylmaleimide I (protein kinase C inhibitors) and E₂ translocated PKC from cytoslic fraction to membrane fraction. In conclusion, E₂ decreased Ca/sup 2+/ uptake and stimulated Pi uptake via cAMP and PKC pathway in the PTCs.

• Molecules and Cells
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• 제22권1호
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• pp.97-103
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• 2006

Phosphoinositides are critical regulators of ion channel and transporter activity. There are multiple isomers of biologically active phosphoinositides in the plasma membrane and the different lipid species are non-randomly distributed. However, the mechanism by which cells impose selectivity and directionality on lipid movements and so generate a non-random lipid distribution remains unclear. In the present study we investigated which structural elements of phosphoinositides are responsible for their subcellular location and movement. We incubated phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PI(4)P) and phosphatidylinositol 4,5-bisphosphate ($PI(4,5)P_2$) with short or long acyl chains in CHO and HEK cells. We show that phosphate number and acyl chain length determine cellular location and translocation movement. In CHO cells, $PI(4,5)P_2$ with a long acyl chain was released into the cytosol easily because of a low partition coefficient whereas long chain PI was released more slowly because of a high partition coefficient. In HEK cells, the cellular location and translocation movement of PI were similar to those of PI in CHO cells, whereas those of $PI(4,5)P_2$ were different; some mechanism restricted the translocation movement of $PI(4,5)P_2$, and this is in good agreement with the extremely low lateral diffusion of $PI(4,5)P_2$. In contrast to the dependence on the number of phosphates of the phospholipid head group of long acyl chain analogs, short acyl chain phospholipids easily undergo translocation movement regardless of cell type and number of phosphates in the lipid headgroup.

• 폴리머
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• 제38권5호
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• pp.632-639
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• 2014

Trimellitic anhydride chloride와 2,7-dihydroxynaphthalene을 이용하여 2,7-dihydroxynaphthalene bis(trimellitate anhydride) (2,7-TA)의 무수물 단량체를 합성하였다. 합성된 2,7-TA와 p-xylylenediamine 및 2,2'-bis(trifluoromethyl) benzidine(TFB)을 다양한 몰 비로 반응하여 얻은 폴리아믹산(polyamic acid, PAA)을 유리판에서 열처리하여 에스터기를 가지는 폴리이미드 공중합체(copolyimide, Co-PI)를 합성하였다. 합성된 Co-PI는 TFB의 몰 비 조성에 따라 열적 성질, 가스 투과도, 및 광학 성질 등을 조사하였다. 용액 캐스팅으로 합성된 Co-PI 필름은 유연하고 질긴 성질을 보였다. Co-PI 필름은 모두 투명하였으며, 각 필름의 cut-off wavelength은 370~395 nm이었고, 노란색 지수는 3.55~7.63의 비교적 낮은 값을 보여주었다. Co-PI 필름의 열적 성질들은 TFB의 몰 비가 증가할수록 증가하였지만, 산소 차단성과 광학 투명성에서는 반대의 결과를 보여주었다.

• 대한약침학회지
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• 제19권3호
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• pp.253-258
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• 2016

Objectives: The goals of this research were to evaluate acute (single-dose) and sub-acute (repeated-dose) toxicity profiles of methanolic extract of Pistacia integerrima J. L. Stewart ex Brandis (PI) for Wistar rats and to assess the safety profile of PI by observing physiological changes, mortality, changes in body weight, the histopathology of body organs, the hematology and the biochemistry of the animals. Methods: The toxicity profile of PI was evaluated using Wistar rats of both sexes. Animals were divided into four groups: Group 1; control group (normal saline), Group 2; PI-1 (250 mg/kg), Group 3; PI-2 (500 mg/kg), Group 4; PL-3 (1,000 mg/kg). An acute-toxicity study in which animals received a single dose of PI extract (2,000 mg/kg) and were then observed for 14 days for changes in skin, fur, eye color, mucous membrane secretions and excretions, gait, posture, and tonic or clonic movements was performed according to guideline 425 of the Organization of Economic and Corporation Development (OECD). In the repeated-dose toxicity study (OECD - 407) animals received a daily dose of PI extract for 28 days (4 weeks). The parameters observed in this study include body weight, hematology and biochemistry of the animals. Results: In the acute toxicity study, no mortalities or changes in behavior were noted in the animals. The repeated-dose toxicity study was also devoid of any toxicity in the animals during the 28 days of testing with PI extract. The extract did not alter- the body weight, hematology or biochemistry of the animals. The methanolic extract of PI was to be found safe to the no-observed-adverse-effect-level (NOAEL) for the single-dose and repeated-dose toxicity tests in rats. Conclusion: The methanolic extract of PI was devoid of toxicity; hence, it can be used for various ayurvedic preparations and treatments of diseases.

• 한국우주과학회:학술대회논문집(한국우주과학회보)
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• 한국우주과학회 2010년도 한국우주과학회보 제19권1호
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• pp.40.3-40.3
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• 2010

The most frequently suggested source for Pi2 pulsations in the inner magnetosphere (L < 4) is standing fast-mode waves trapped in the plasmasphere (i.e., plasmaspheric resonances). They have been considered as the source of low-latitude Pi2 pulsations. The plasmaspheric resonance model suggests that compressional fast-mode waves can be detected at all local times inside the plasmasphere provided reflection of the wave energy is efficient. Until now, however, there are no reports about compressional Pi2s observed in the dayside inner magnetosphere. That is, there is longitudinal limit of inner magnetosphere. In February 2008, THEMIS probes were near dawn and/or dusk sides, which are the transition regions between the nightside and dayside, in the inner magnetosphere (L = 2-4) when low-altitude Pi2s were identified at Bohyun (L = 1.35) station in Korea. Using the THEMIS electric field data, we examined if Pi2s are excited by longitudinally localized disturbances. We found that compressional Pi2s having high coherence with a low-latitude Pi2 pulsation occur on dawnside. However, any compressional pulsations in the Pi2 frequency band were not detected on duskside. This indicates that compressional Pi2s disappear near the duskside. Our observations are discussed with spatial plasmaspheric structure and possible Pi2 mechanisms.

• 조명전기설비학회논문지
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• 제15권3호
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• pp.57-66
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• 2001

본 논문에서는 전력계통의 안정도를 향상시키기 위하여 동기 발전기와 정지형 무효전력 보상기예 대한 퍼지-PI 제어기를 설계하기 위한 제어 기법을 설명하였다. 정지형 무효전력 보상기는 고정된 용량의 커패시터와 싸이리스터 제어에 의하여 용량이 가변되는 인덕터가 병렬로 연결된 구조를 가지고 있으며, 시스템 전압을 제어할 뿐만 아니라 동기 발전기의 제동을 개선하기 위해 설계되었다. 본 논문에서 제안한 SVC 계통의 퍼지-PI 제어기의 파라미터는 퍼지 추론 기법에 의해 자동 동조되어진다. 퍼지 추론 기법은 일반적인 기법과는 달리 인간의 경험과 전문가의 지식을 제어 규칙으로 제어 동작을 결정하였다. 그리하여 인간의 추론 과정과 매우 유사한 MMGM을 이용하여 PI 이득의 퍼지 추론 기법을 SVC 계통에 적용하여 설명하였다. 제안된 방법의 강인성을 입증하기 위해 중부하시, 정상부하시 및 경부하시에 초기 전력을 변동시킨 경우에 대하여 시스템의 회전자각, 각속도 편차 특성 및 단자전압의 동특성을 고찰하여 기존의 전력시스템안정화장치보다 응답특성이 우수함을 보였다.

• 반도체디스플레이기술학회지
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• 제18권4호
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• pp.30-34
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• 2019

We analyzed how the features in plasma information based virtual metrology (PI-VM) for SiO₂ etching depth with variation of 5% contribute to the prediction accuracy, which is previously developed by Jang. As a single feature, the explanatory power to the process results is in the order of plasma information about electron energy distribution function (PI_EEDF), equipment, and optical emission spectroscopy (OES) features. In the procedure of stepwise variable selection (SVS), OES features are selected after PI_EEDF. Informative vector for developed PI-VM also shows relatively high correlation between OES features and etching depth. This is because the reaction rate of each chemical species that governs the etching depth can be sensitively monitored when OES features are used with PI_EEDF. Securing PI_EEDF is important for the development of virtual metrology (VM) for prediction of process results. The role of PI_EEDF as an independent feature and the ability to monitor variation of plasma thermal state can make other features in the procedure of SVS more sensitive to the process results. It is expected that fault detection and classification (FDC) can be effectively developed by using the PI-VM.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2014년도 춘계학술대회
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• pp.429-431
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• 2014

라즈베리 파이는 영국 라즈베리 파이(Paspberry Pi) 재단에서 만든 초소형/초저가 PC이며 교육용 프로젝트의 일환으로 개발되었다. RCA 연결 잭을 가지고 있으며 2012년 3월에 출시되어 1시간만에 모두 매진되기도 했다. 라즈베리 파이는 리눅스 커널 기반 운영체제를 사용하여 Raspbian이라는 라즈베리 파이에 최적화된 데비안 계열의 무료 운영체제를 주로 사용하고 있다. 현재 라즈베리 파이는 큐비 보드와 함께 IoT 분야에서 상당히 각광받고 있으며 큐비 보드보다 많은 자료를 보유하고 있다. 아두이노는 상당히 쉬운 접근을 허용하지만 avr의 접근성 및 코드 연계등이 불가능하여 모든 커스터마이징을 라이브러리로만 해결해야하는 단점이 존재한다. 라즈베리파이는 라즈비안을 사용하며 가격이 저렴하지만 입출력이 작은 한계점이 존재한다. 그래서 이 둘의 결합을 위해 GPOI를 사용한 하드웨어 제어를 생각하게 되었다. 본 논문에서는 GPIO를 사용한 RaspberryPi 보드를 제어하기 위해 Cooking Hacks 실드를 사용하여 입출력이 부족한 라즈베리 파이 확장을 확인하였고 잘 동작됨을 검증하였다.

• International Journal of Air-Conditioning and Refrigeration
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• 제10권2호
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• pp.106-115
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• 2002

The performance of an inverter-driven water-to-water heat pump with an electronic expansion valve (EEV) was measured as a function of compressor frequency, load conditions, and EEV opening. Based on the test results, a controller using proportional integral (PI) feedback or PI feedforward algorithm was designed and tested to investigate capacity modulation and transient response control of the system. Although the relation between superheat and EEV opening of the heat pump showed nonlinear characteristics, a control gain obtained at the rated frequency was applicable to various operating conditions without causing large deviations. When the simple PI feedback control algorithm was applied, a large overshoot of superheat and wet compression were observed due to time delay effects of compressor frequency. However, applying PI feedforward control scheme yielded better system performance and higher reliability, compared to the PI feedback algorithm.