• Asian Pacific Journal of Cancer Prevention
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• v.16 no.5
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• pp.2031-2035
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• 2015

Intra-tumoral hypoxia is an environment that promotes tumor cell migration, angiogenesis and epithelial-mesenchymal transition that accounts for a major mechanism of metastasis. Chloroquine potentially offers a new therapeutic approach with an 'old' drug for effective and safe cancer therapies, as it exerts anti-metastatic activity. We investigated the inhibitory effect of chloroquine on cholangiocarcinoma (CCA) cell migration under cobalt chloride ($CoCl_2$)-stimulated hypoxia. We showed that chloroquine suppressed CCA cell migration under hypoxic-mimicking conditions on exposure to $100{\mu}M$ $CoCl_2$. Moreover, chloroquine stabilized the protein level of prolyl hydroxylase domain proteins (PHD-2) but reduced the levels of hypoxic responsive proteins such as hypoxia-inducible factor (HIF-$1{\alpha}$) and vascular endothelial growth factor (VEGF). It also suppressed epithelial mesenchymal transition (EMT) by increasing the ratio of E-cadherin to N-cadherin under hypoxic conditions. In conclusion, chloroquine can inhibit hypoxia-stimulated metastasis via HIF-$1{\alpha}$/VEGF/EMT which may serve as a useful additional strategy for CCA therapy.

• Korean Journal of Animal Reproduction
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• v.19 no.2
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• pp.89-93
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• 1995

In this study was demonstrate that retrovirus-mediated gene transfer is one of the promising alternatives to the conventional pronuclear DNA microinjection approach, especially in transferring the exogenous genes into the boving embryos. By co-culturing of zona of zona-free one-cell stage embryos with the retrovirus-producing cells for 24 hours followed by 6 days of culture in virus-free medium, we could get morulae and blastocysts expressing the E. coli LacZ genes which were transferred by our retrovirus vector. The results obtained in this study are summarized as follows : 1. Addition of 5$\mu\textrm{g}$/ml of polybrene in the embryo and virus-producing cell co-culture medium did not affect development of zona-free one-cell embryo. 2. Compared with the intact embryos removal of zona at one-cell stage before co-culturing with the virus-producing cells for one day caused only slight decrease of embryo develpment. 3. Co-culture of 625 zona-free one-cell stage embryos with the virus-producing cells resulted in 65(10.4%) morulae or blastocysts, and 12.3%(8/65) of the morulae or blastocysts were E. coli LacZ positive.

• KSBB Journal
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• v.26 no.3
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• pp.189-192
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• 2011

Much interest has been recently focused on the production of large quantities of hydrogen, due to its potential importance in our economy and needs in the petroleum and chemical industries. Formate dehydrogenase H (FDH-H) from Escherichia coli containing selenocysteine that oxidizes formate to carbon dioxide with the release of hydrogen is a component of the anaerobic formate hydrogen-lyase complex of E. coli. To make full use of FDH-H, we need effective expression condition. In this approach, we investigated the effect of pH on FDH-H stability and observed the effect of selenite and formate concentration on the activity of FDH-H. Additionally, coexpression of selenocysteine insertion genes were tried to improve the expression of FDH-H. The highest level of FDH-H expression was achieved by coexpression of selenocysteine insertion genes (pSUABC) as well as by the addition of $10\;{\mu}M$ selenite and 10 mM formate. At this optimized condition, a 2.6 fold elevation of expression of FDH-H was achieved.

• Journal of Environmental Impact Assessment
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• v.2 no.2
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• pp.111-116
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• 1993

The EIA system was developed during ten odd years of introduction. On the other hand, the contentional development representing systematical fidelity does not match to it. I surveyed the problems of EIA on the point of environmental items with no legal standards. EIA is being executed only once on the planning stage. If the present EIA system has two times of execution, the quality of EIA will be much better. First, on the stage of deciding location of the project, EIA should be done. And next, detailed EIA should be done on the stage of executing the project. To describe the problems of EIA, I surveyed the legislations on the point of the former stages, investigation of present status and anticipation. And I tried a theoretical approach to EIA. The knowledge of things, the subject's standpoint and his valuation are all put together in the EIA. The knowledge of relationship between air and water pollutants and their impact on human is accumulated much. On the other hand, the knowledge of the compounded item, fauna-flora(natural ecology) is partial and causes many difficulties. I pointed out the absurdities of assessment technique dealing the standards and introduced assessment technique being applied to items with no physico-chemical standards such as wild lives.

• Applied Microscopy
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• v.43 no.1
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• pp.34-39
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• 2013

Glandular and nonglandular trichomes on the leaf surface of Plectranthus tomentosa were investigated by variable pressure field emission scanning electron microscopy (VP-FESEM). The segments of the plant's leaves were directly mounted without any specimen preparation, and examined at ambient temperature using a variable pressure secondary electron (SE) detector under ca. 15 Pa. Foliar trichomes maintained their shapes and structures without severe surface collapse or charging. The adaxial leaf surface was abundantly covered with different types of trichome. Nonglandular trichomes consisted of a basal cell and a long (up to ca. $300{\mu}m$) stalk. Meanwhile, capitate glandular trichomes had a secretory head and a short or long stalk. Peltate glandular trichomes with globose secretory heads were observed in close contact with the leaf epidermis. Spherical projections on the secretory head showed the secretion process of glandular trichomes. In addition to the trichomes, oval stomata were distributed on the abaxial leaf surface. These results suggest that ambient VP-FESEM can be used to classify the dehydration-sensitive foliar trichomes of succulent plants by SE imaging. At the FESEM resolution, this approach facilitates the rapid and detailed morphological analysis of a variety of trichomes in diverse plant taxa with reduced labor and preparation.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.8
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• pp.12-19
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• 2004

Low-speed gas flows around a micro-scale flat plate are investigated using a kinetic theory analysis. The Boltzmann equation simplified by a collision model is solved by means of a finite difference approximation with the Discrete Ordinate method. Calculations are made for flows around a 5% flat plate with a finite length of 20 microns. The results are compared with those from the Information Preservation method and a continuum approach with slip boundary conditions. It is shown that three different approaches predict a similar basic flow patterns, while the results from the present method are more accurate than those from the other two methods in details.

• Journal of Korean Society for Atmospheric Environment
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• v.27 no.2
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• pp.133-141
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• 2011

In this study, different calibration approaches for reduced sulfur compounds (RSCs) were investigated by using thermal desorption coupled with gas chromatography (GC) and pulsed flame photometric detection (PFPD). To evaluate the effects of calibration procedures, gaseous standards of 4 RSCs ($H_2S$, $CH_3SH$, DMS, and DMDS) prepared at 10 ppm level were analyzed at 7 loading injection volumes (40, 60, 80, 100, 160, 240, and 320 ${\mu}L$). The results were then compared with calibration curves made with the Z (zero offset) and N (non-zero offset) method. The concentrations of unknown samples were then quantified by using R (ratio) method in which the slope values are compared between standards and samples. Secondly, in A (average) method, results obtained from a multi-point analysis of unknown samples were also averaged to extract representative values for each sample. Results of both experiments showed that analytical error of low molecular weight components (such as $H_2S$ and $CH_3SH$) was greatly expanded with the Z method. In conclusion, the combined application of N-A method was the more realistic approach to reduce biases in the quantification of RSCs.

• Journal of the Korean Applied Science and Technology
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• v.28 no.1
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• pp.102-108
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• 2011

The study of wave propagation and scattering in biological media has become increasingly important in recent years. The propagation of light within tissues is an important problem that confronts the dosimetry of therapeutic laser delivery and the development of diagnostic spectroscopy. In the clinical application of photodynamic therapy(PDT) and in photobiology, the photon deposition within a tissue determines the spatial distribution of photochemical reactions. Scattered light is measured as a function of the distance (r) between the axis of the incident beam and the detection spot. Consequently, knowledge of the photosensitizer(Chlorophyll-a) function that characterizes a phantom is measured. To obtain the results of scattering coefficients(${\mu}s$) of a turbid material from diffusion described by experimental approach. It was measured the energy fluency of photon radiation at the position of penetration depth. From fluorescence experimental method obtained the analytical expression for the scattered light as the values of $(I/I_o)_{wavelength}$ vs the distance between the center of the incident beam and optical fiber in terms of the condition of "in situ spectroscopy(optically thick)" and real time by fluorometric measurements. The result was compromised with transport of intensities though a random distribution of scatters.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2004.05a
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• pp.23-26
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• 2004

Recently, most of countries in the world are investing huge amount of capital for the infrastructure of logistics and trying to gain dominating position in logistics. To play the role of important hub in logistics, an efficient, flexible, and fault-tolerant transportation process should be developed. Minimization of transportation cost and timely deliveries in the unpredictable environment are a few of the important issues in logistics. This study suggests a way of transporting goods to destinations at the minimal cost and with the minimal delay by optimally allocating transporting resources. Various attributes in transportation such as due date, priority etc. are also considered. Appropriate transporting resources for each work item is selected by calculating the weighted sum of the cost factor and the delay factor assuming that initial sequences of work items are given. A policy to reallocate transporting resources is also suggested when work items or transporting resources are added or deleted because of accidents or disturbances. This policy provides adaptability to the allocation methodology which enables the system to cope with changing environment by controlling various attributes in transportation. Genetic algorithm is used for this approach.

• Proceedings of the Korean Operations and Management Science Society Conference
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• 2005.05a
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• pp.1124-1128
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• 2005

In this paper, a plan-coordination architecture is proposed for multi-agent control in the fractal manufacturing system (FrMS). A fractal in FrMS is a set of distributed agents whose goal can be achieved through cooperation, coordination, and negotiation with other agents. Since each agent in the FrMS generates, achieves, and modifies its own plan fragments autonomously during the coordination process with other agents, it is necessary to develop a systematic methodology for the achievement of global plan in the manufacturing system. The heterarchical structure of the FrMS provides a compromised plan-coordination approach, it compromise a centralized plan-generation/execution (which mainly focuses on the maximization of throughput) with a distributed one (which focuses on the autonomy of each module and flexibility of the whole system). Plan-coordinators in lower level fractal independently generate plan fragments according to the global plan of higher level fractal, and plan-coordinators in higher level fractal mediate/coordinate the plan fragments to enhance the global performance of the system. This paper assumes that generation method of the plan fragments and the negotiation policy of the fractal is achieved by a simple process, and we mainly focuses on the information exchanging and distributed decision making process to coordinate the combinations of plan fragments within a limited exchange of information.