• IMMUNE NETWORK
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• v.5 no.3
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• pp.163-171
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• 2005

Background: To perform the successful dendritic cell-based cancer immunotherapy one of the main issues to be solved is the source of antigen for DC pulsing. Limitations occur by using auto-tumor lysate due to the difficulties obtaining enough tumor tissue(s) quantitatively as well as qualitatively. In this study the possibility of allogeneic tumor cell lysate as a DC pulsing antigen has been tested in mouse melanoma pulmonary me tastasis model. Methods: B16F10 melanoma cells $(1{\timeS}10^5/mouse)$ were inoculated intra venously into the C57BL/6 mouse. Therapeutic DCs were cultured from the bone marrow myeloid lineage cells with GM-CSF and IL-4 (1,000 U/ml each) for 7 days and pulsed with lysate of either autologous B16F10 (B-DC), allogeneic K1735 (C3H/He origin; K-DC) or CloneM3 (DBA2 origin; C-DC) melanoma cells for 18 hrs. Pulsed-DCs $(1{\times}10^6/mouse)_{[CGP1]}$ were injected i.p. twice with one week interval starting from the day 1 after tumor cell inoculation. Results: Without observable toxicity, allogeneic tumor cell lysate pulsed-DC induced the significantly better anti-tumor response (tumor scale: $2.7{\pm}0.3,\;0.7{\pm}0.3\;and\;0.3{\pm}0.2$ for saline, B-DC and C-DC treated group, respectively). Along with increased tumor specific lymphocyte proliferations, induction of IFN-${\gamma}$ secretion against both auto- and allo-tumor cell lysates was observed from the DC treated mice. (w/B16F10-lysate: $44.97{\pm}10.31,\;1787.94{\pm}131.18,\;1257.15{\pm}48.27$, w/CloneM3 lysate: 0, $1591.13{\pm}1.83,\;1460.47{\pm}86.05pg/ml$ for saline, B-DC and C-DC treated group, respectively) Natural killer cell activity was also increased in the mice treated with tumor cell lysate pulsed-DC ($8.9{\pm}_{[CGP2]}0.1,\;11.6{\pm}0.8\;and\;12.6{\pm}0.7%$ specific NK activity for saline, B-DC and C-DC treated group, respectively). Conclusion: Conclusively, promising data were obtained that allogeneic-tumor cell lysate can be used as a tumor antigen for DC-based cancer immunotherapy.

• Progress in Medical Physics
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• v.19 no.3
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• pp.150-156
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• 2008

An applied technique of contrast enhancement for X-ray image is proposed which is based on combined enhancement of scaling and wavelet coefficients in discrete wavelet transform space. Conventional contrast enhancement methods such as contrast limited adaptive histogram equalization (CLAHE), multi-scale image contrast amplification (MUSICA) and gamma correction were applied on scaling coefficients to enhance the contrast of an original. In order to enhance the detail as well as reduce the blurring caused by up scaling of contrast modified scale coefficients from lower resolution, the sigmoid manipulation function was used to manipulate wavelet coefficients. The contrast detail mammography (CDMAM) phantom was imaged and processed to measure the image line profile of results and contrast to noise ratio (CNR) comparatively. The proposed technique produced better results than direct application of various contrast enhancement methods on image itself. The proposed method can enhance contrast, and also suppress the amplification of noise components in a single process. It could be useful for various applications in medical, industrial and graphical images where contrast and detail are of importance.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2015.05a
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• pp.951-953
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• 2015

Learning for thinking says the behavior of the organism changes as a result of practice or experience. It is very difficult to identify focusing ability objectively when students study. But, brain of the body is not so. EEG signal means continuously electric records of brain potential variation between two points on the scalp when brain activities take place. In types of EEG, there are delta(0~4Hz), theta(4~8Hz), alpha(8~13Hz), beta(13~30Hz) and gamma waves(30~50Hz). SMR waves and Mid-beta waves appear when focused for studying. Part for the most influence on concentrating reported that Mid-beta waves. In relation to brain activities, EEG has been actively researched for evaluating brain focus index system during learning and study. So, By using Biopac system for this study, measured brain wave was converted into FFT for extracting Mid-beta domain signals that are related to learning after giving focus invoked subjects to a small number of people. When concentrating, we measured the change in the power of the Mid-beta frequency domain and presented a correlation. Based on these results, we analyzed whether students are concentrated objectively on learning or not. and hope to offer more efficient learning method.

• Investigative Magnetic Resonance Imaging
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• v.4 no.1
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• pp.7-13
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• 2000

Purpose : To assess the distortion of MRI with the Leksell stereotactic radiosurgery system in variable pulse sequence and imaging plane through phantom study, to find most adequate imaging plane and pulse sequence for stereotactic radiosurgery system. Materials and methods : We made the phantoms for MRI and get images in variable conditions and analyzed the image distortion using image analysis program, and statistically using paired student t-test. Results : The transeverse plane images had acceptable error ranges bless than 1.5mm) in all pulse sequence in both the analysis of fiducial marker in stereotactic G-frame and the phantom study. The coronal plane images had unacceptable large errors (more than 1.7mm) in the analysis of fiducial marker in the stereotactic G-frame, but had corrected small errors (less than 1.5mm) in the phantom study. Conclusion : We find from the phantom study that the present MR machines are adequate for stereotactic surgery system in frequently used pulse sequences, and imaging planes.

• Atmosphere
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• v.24 no.1
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• pp.49-68
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• 2014

This study analyzed atmospheric conditions for the convergent cloud band (Cu-Cb line) in developing stage and its neighbouring convections formed over the East Sea on 1 February 2012, by using synoptic, satellites data, and WRF numerical simulation output of high resolution. In both satellite images and the WRF numerical simulation outputs, the Cu-Cb line that stretched out toward northwest-southeast was shown in the East Sea, and cloud lines of the L mode were aligned in accordance with the prevailing surface wind direction. However, those of the T mode were aligned in the direction of NE-SW, which was nearly perpendicular direction to the surface winds. The directions of the wind shear vectors connecting top winds and bottom winds of the moist layers of the L mode and the T mode were identical with those of the cloud lines of L mode and T mode, respectively. From the WRF simulation convection circulations with a convergence in the lower layer of atmosphere and a divergence above 1.5 km ASL (Above Sea Level) were identified in the Cu-Cb line. A series of small sized vortexes (maximum vortex: $320{\times}10^{-5}s^{-1}$) of meso-${\gamma}$-scale formed by convergences was found along the Cu-Cb lines, suggesting that Cu-Cb lines, consisting of numerous convective clouds, were closely associated with a series of the small vortexes. There was an absolute unstable layer (${\partial}{\theta}/{\partial}z$ < 0) between sfc and ~0.3 km ASL, and a stable layer (${\partial}{\theta}/{\partial}z$ > 0) above ~2 km ASL over the Cu-Cb line and cloud zones. Not only convectively unstable layers (${\partial}{\theta}_e/{\partial}z$ < 0) but also neutral layers (${\partial}{\theta}_e/{\partial}z{\approx}=0$) in the lower atmosphere (sfc~1.5 km ASL) were scattered around over the cloud zones. Particularly, for the Cu-Cb line there were convectively unstable layers in the surface layer, and neutral layers (${\partial}{\theta}_e/{\partial}z{\approx}=0$) between 0.2 and ~1.5 km ASL over near the center of the Cu-Cb line, and the neutralization of unstable layers came from the release of convective instability.

• Korean Journal of Food Science and Technology
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• v.31 no.4
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• pp.906-911
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• 1999

This study was to determine whether DNA 'Comet Assay' can be applied to the detection of grains irradiated with low doses of Co-60 gamma radiation. Sesame, perilla, wheat, barley and rice were exposed to different doses of 0.1, 0.3, 0.5, 0.7 and 1.0 kGy. The cells isolated from the samples were embedded in a agarose gel on a microscope slide, lysed in lysis solution, and subjected to electrophoresis. DNA and its fragments migrated in the gel produced the characteristic pattern of DNA comet, of which the tail length was measured in a microscope. All the samples irradiated at 0.3 kGy and higher were applicable to detect post-irradiation by the tail length of their comets. Irradiated samples showed comets with long tails and their tail length increased with the dose, while unirradiated samples showed no or very short tails. Especially, sesame, perilla and wheat irradiated at 0.1 kGy could be distinguished from unirradiated samples by visual inspection of the slide in a microscope. Thus, DNA 'Comet Assay' might be applied to the detection of irradiated grains as a simple, inexpensive and rapid screening test.

• Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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• 2003.04a
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• pp.388-393
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• 2003

도시화 속도의 증가와 더불어 3차원 자료 획득의 출처가 다양해지연서, 도로 및 건물경계선과 같은 선형 GIS 정보에 대한 신속한 갱신 또한 요구되고 있다. 임의의 출처 자료로부터 대상 자료를 갱신하기 위해서는 가장 먼저 두 자료간의 위치 관계를 결정하여야 하며, 특히 영상정보와 같은 출처 자료와 GIS 자료와 같은 대상 자료간의 위치 관계를 결정하기 위하여 기존에 제시되어온 대부분의 방법들은 두 개 자료간의 관계를 정의 할 수 있는 기준정과 같은 정확한 점 정합 요소(point matching entities)를 요구하고 있다. 따라서 정확한 정합 요소들을 획득할 수 없는 경우 영상과 GIS 자료간의 위치 관계를 결정할 수 없을뿐더러 위치 관계 정립의 결과는 정합 요소들의 분포 및 정확도에 매우 의존하게 된다. 또한 이러한 점 정합 요소들을 정의하기 위해서는 대부분의 경우 수동적으로 이루어질 수밖에 없다. 따라서 본 연구에서는 영상 및 GIS 자료의 선형 정보를 이용하여 정확한 점 정합 요소들을 모르더라도 영상과 GIS 자료간의 위치 관계를 결정할 수 있는 기법을 제시하고자 한다. 사용된 알고리즘은 개선된 Hough 변환(Modified Hough Transform)을 기반으로 다수의 선형 정보 중에 정합되는 요소들을 자동으로 찾아내고 이들을 최소제곱법으로 풀이함으로써 두 데이터간의 기하학적 변환 관계를 결정하는 기법이다. 본 연구에서는 이와 같은 접근을 통해 데이터간의 기하학적 변환 관계를 결정한 후, 영상 상에는 존재하지만 GIS 자료에는 존재하지 않는 선형 정보에 대한 갱신 여부를 확인하고 갱신함으로써 3차원 위치 자료의 자동 생성에 대한 가능성을 제시하고자 한다.로 갈수록 퇴적이 우세한 것으로 관측되었다.보체계의 구축사업의 시각이 행정정보화, 생활정보화, 산업정보화 등 다양한 분야와 결합하여 보다 큰 시너지 효과와 사용자 중심의 서비스 개선을 창출할 수 있는 기반을 제공할 것을 기대해 본다.. 이상의 결과를 종합해볼 때, ${\beta}$-glucan은 고용량일 때 직접적으로 또는 $IFN-{\gamma}$ 존재시에는 저용량에서도 복강 큰 포식세로를 활성화시킬 뿐 아니라, 탐식효율도 높임으로써 면역기능을 증진 시키는 것으로 나타났고, 그 효과는 crude ${\beta}$-glucan의 추출조건에 따라 달라지는 것을 알 수 있었다.eveloped. Design concepts and control methods of a new crane will be introduced in this paper.and momentum balance was applied to the fluid field of bundle. while the movement of′ individual material was taken into account. The constitutive model relating the surface force and the deformation of bundle was introduced by considering a representative prodedure that stands for the bundle movement. Then a fundamental equations system could be simplified considering a steady state of th

• Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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• 2003.04a
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• pp.510-516
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• 2003

연안관리 정책을 결정하기 위해서는 다양한 정보의 수집과 이를 체계적으로 관리하고 분석할 수 있는 기법이 필요하다. 특히, 다양한 이해집단과 목적들이 상충하는 지역의 특성을 파악하기 위해서는 환경, 사회, 경제적인 기준 및 의사결정자의 가치체계가 반영될 수 있어야 하며, 선호도가 반영된 요소를 효율적으로 분석할 수 있어야 한다. 이에, 본 연구에서는 공간자료의 처리 및 분석이 용이한 GIS 환경에 다기준 분석법, 그 중에서도 AHP 모형을 결합하는 방법을 다루어 보았다. 분석과정을 살펴보면, 본 연구에서는 지역에 영향을 미치는 사회ㆍ경제적인 지표, 환경 지표를 정하였고, 이런 지표들의 데이터를 GIS라는 도구를 통하여 분석이 용이하도록 변환시켰다. 한편, 이 지역에 영향을 미치는 각 기준들의 중요성을 파악하여 의사결정자의 의견을 반영할 수 있도록 다기준 분석법의 하나인 AHP를 이용하여 가중치를 산정하였다. 다음으로, 다양한 출처의 자료를 표준화하여 GIS의 래스터 자료로 구축한 후, 가중치를 적용한 개별 레이어를 지도대수와 중첩분석을 이용하여 최종 결과 레이어를 생성하였다. 생성된 최종 결과 레이어 상의 공간의 대안인 각 셀 값을 비교ㆍ분석하였다. 이로 인한 결과는 연안의 유한한 자원과 공간의 다양한 이용상태를 관리하기 위한 해안과 육상의 정보를 제공할 수 있다. GIS와 다기준 분석을 통합함으로써 다양한 출처의 공간정보를 분석하고 연안의 현 상태를 밝힐 수 있다. 또한, 이것은 분석 결과가 단순하고 명확하게 설명되어 정책 결정자에게 유용한 정보를 제공할 뿐만 아니라, 이 정보를 이용하여 실질적인 연안관리계획을 수립하는데 도움이 된다.가능성 0.5이상의 면적은 59%를 차지하였다.퇴적이 우세한 것으로 관측되었다.보체계의 구축사업의 시각이 행정정보화, 생활정보화, 산업정보화 등 다양한 분야와 결합하여 보다 큰 시너지 효과와 사용자 중심의 서비스 개선을 창출할 수 있는 기반을 제공할 것을 기대해 본다.. 이상의 결과를 종합해볼 때, ${\beta}$-glucan은 고용량일 때 직접적으로 또는 $IFN-{\gamma}$ 존재시에는 저용량에서도 복강 큰 포식세로를 활성화시킬 뿐 아니라, 탐식효율도 높임으로써 면역기능을 증진 시키는 것으로 나타났고, 그 효과는 crude ${\beta}$-glucan의 추출조건에 따라 달라지는 것을 알 수 있었다.eveloped. Design concepts and control methods of a new crane will be introduced in this paper.and momentum balance was applied to the fluid field of bundle. while the movement of′ individual material was taken into account. The constitutive model relating the surface force and the deformation of bundle was introduced by considering a representative prodedure that stands for the bundle movement. Then a fundamental equations system could be sim

• The Journal of the Petrological Society of Korea
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• v.19 no.4
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• pp.283-292
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• 2010

The Suljeongri east three-story stone pagoda in Changnyeong (National Treasure No. 34) has been damaged mainly by lots of cracks. The stones used for this pagoda are medium-granied equigranular pinkish biotite granite. Measured magnetic susceptibility values are of from 2 to 9 (${\times}10^{-3}$ SI unit). From the ${\gamma}$-ray spectrometer mesurement K, eU, and eTh contents of the stones are 3 to 7%, 8 to 19 ppm, and 11 to 35 ppm, respectively. Comparing the petrographical and chemical characteristics between the stones of the pagoda and the country rocks near Suljeongri, it is suggested that the most similar rock could be equigranular biotite granite in the western slope of the Mt. Hwawangsan. Vertical, horizontal and diagonal cracks are intensely developed at the lower part of the stone pagoda. Biotite granite has intrinsic microcracks defined as rift and grain rock cleavages. Both rock cleavages are assumed to have led to the crack growth and consequent mechanical damage of the pagoda. It seems that vertical cracks have been grown parallel to the principal compressional stress, and that horizontal cracks to the reacting tensional stress. Diagonal cracks seems likely to have been resulted from conjugate rift and grain rock cleavages.

• Journal of Radiation Protection and Research
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• v.24 no.4
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• pp.205-213
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• 1999

Effective dose conversion coefficients from unit activity radionuclides contaminated on the ground surface were calculated by using MCNP4A rode and male/female anthropomorphic phantoms. The simulation calculations were made for 19 energy points in the range of 40 keV to 10 MeV. The effective doses E resulting from unit source intensity for different energy were compared to the effective dose equivalent $H_E$ of previous studies. Our E values are lower by 30% at low energy than the $H_E$ values given in the Federal Guidance Report of USEPA. The effective dose response functions derived by polynomial fitting of the energy-effective dose relationship are as follows: $f({\varepsilon})[fSv\;m^2]=\;0.0634\;+\;0.727{\varepsilon}-0.0520{\varepsilon}^2+0.00247{\varepsilon}^3,\;where\;{\varepsilon}$ is the gamma energy in MeV. Using the response function and the radionuclide decay data given in ICRP 38, the effective dose conversion coefficients for unit activity contamination on the ground surface were calculated with addition of the skin dose contribution of beta particles determined by use of the DOSEFACTOR code. The conversion coefficients for 90 important radionuclides were evaluated and tabulated. Comparison with the existing data showed that a significant underestimates could be resulted when the old conversion coefficients were used, especially for the nuclides emitting low energy photons or high energy beta particles.