• The Journal of Dong Guk Oriental Medicine
• /
• v.5
• /
• pp.53-77
• /
• 1996

The purpose of this study is to observe the effect of Sayeuksan on serum reaction in $CCl^4$ treated rats. In this study the experimental rats divided four group (Control group, $CCl^4$ group, sayeuksan group, and shiho, kamcho group) Under the same condition control group were administered water, sample group were administered Sayeuksan for 8days. and then, both $CCl_4$ group and sample group were injeccted to abdomen with $CCl_4$ for 1days. The change of GOT GPT ${\gamma}$-GTP ALP LDH activity in blood serum. The obtained results are summarised as follows : 1. In the change of GOT GPT contents, as compared with control group sample group was significantly decreased. 2. In the change of ${\gamma}$-GTP contents as compared with control group, sample group was significantly decreased. 3. In the change of ALP contents, as compared with control group, sample group was significantly decreased. 4. In the change of LDH contents, as compared with contol group, sample group was significantly decreased.

• Korean Journal of Acupuncture
• /
• v.17 no.1
• /
• pp.19-31
• /
• 2000

Effects of acupuncture at Taekjung and Ungyo($GV_{28}$) bloodletting on serum glutamic oxaloacetic transaminase (GOT) activity, glutamic pyruvic transaminase activity(GPT), total Protein, Albumin, Alkalin Phosphatase(ALP), ${\gamma}$-GT, lactic dehydrogenase (LDH), total cholesterol and triglyceride were determined. 1) In the Ungyo bloodletting group, GOT, GPT, ALP, ${\gamma}$-GT and LDH activity showed a tendency to decrease compared to those of $CCl_4$ only group and Taekjung acupuncture group. 2) Serum albumin content showed a tendency decrease by treatment of $CCl_4$ and to increase by treatment of Ungyo bloodletting. 3) The values of serum total protein, total cholesterol and triglyceride showed no significant difference among all the treatment groups. 4) All the biological values showed no significant difference between the $CCl_4$ only group and Taekjung acupuncture group except the values of albumin. Results from this study indicate that the Ungyo bloodletting showed the possibility of liver function recovery in liver-harmed rats by $CCl_4$.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.22 no.2
• /
• pp.116-126
• /
• 1993

The purposes of this study were to investigate the effect of seleniumc (Se) and vitamin E on activity of enzyme relevant to lipid peroxidation in alcohol administrated rats. Seventy two male rats of Sprague-Dawley strain weighing about 58~62g were divided into 12groups. The dietary Se levels were 0, 0.4 and 10mg and the dietary vitamin E levels were 0 and 150mg per kg diet, respectively. Alcohol-administrated groups received drinking water solution containing 10% of ethanol from the 3-weeks of experimental periods. The obtained experimental results are summarized as follow: The ${\gamma}$-GTP activity in plasma was higher in alcohol administrated groups and high selenium group (HSe) and low selenium group (LSe) than in control groups (CSe). The ${\gamma}$-GOT and GPT activities were higher in alcohol groups. The ${\gamma}$-GTP activity was significantly influenced by alcohol in LSe groups than in other groups. The glutathione peroxidase (GSH-Px) activity of plasma was significantly lower in LSe groups than HSe and CSe groups. The GSH-Px activity of microsomal and cytosolic fraction was slightly lower in alcohol groups and was about a half value lower in HSe and LSe groups than CSe groups. There was negative correlation between plasma Se level and GSH-Px activity of cytosolic fraction in HSe groups (r=- 0.662, p<0.001) and positive correlation in LSe groups (r=0.640, p<0.001). The GSH S-transferase activity in microsomal and cytosolic fraction was slightly higher in alcohol administrated but vitamin E nonadministrated groups, and significantly higher in LSe groups than in other groups. The catalase activity in mitochondria was lower in HSe than CSe groups, but rather higher in LSe groups. The superoxide dismutase (SOD) activity in cytosolic fraction of liver was not found any effect in all groups. The cytochrome P-450 was higher in alcohol groups, but significantly lower in HSe groups. In conclusion, the deficiency of Se and vitamin E develops the hyperoxidation of liver lipid through the increase of activity of enzyme related to the lipid peroxidation and alcohol administration appears to further increase of hyperoxidation of liver lipid.

• The Journal of Pediatrics of Korean Medicine
• /
• v.11 no.1
• /
• pp.183-204
• /
• 1997

In order to study the effects of Daebangpungtang on galactosamine induced hepatoxity in rats, the experimental rats divided five groups (Normal, Control, Sample A, B, C groups). Under the same condition, normal and control groups were administered normal saline for 16 days, control group was injected to abdomen with galactosamine at 8th day (800mg/kg). Sample A group was administered the Daebangpungtang aqueous solution($200m{\ell}／kg$ p.o) for 8 days and injected galactosamine(800mg／kg i.p) for the last day and was administered normal saline for 8 days. Sample B group was treated as same as group A for 8 days, and then was administered the Daebangpungtang aqueous solution($200m{\ell}／kg$ p.o) forfurther 8 days. Sample C group was administered the Daebangpungtang aqueous solution($200m{\ell}／kg$ p.o) for 16 days. The activity of GOT, GPT, ${\gamma}$-GTP, ALP, LDH and total bilirubin in serum were measured at 8th and last day. The results were obtained as follows: 1. The activity of serum GOT of the sample A group decreased effectively at the 8th day and sample B group decreased effectively at 16th day as compared with the control group. 2. The activity of serum GPT of the sample A group decreased effectively at the 8th and 16th day and sample B group decreased effectively at 16th day as compared with the control group. 3. The activity of serum ${\gamma}$-GTP of the sample B group decreased effectively at the 16th day as compared with the control group. 4. The activity of serum ALP of the sample A group increased respectively at the 8th and 16th day and sample B group decreased effectively at 16th day as compared with the control group. 5. The activity of serum LDH of the sample A, B groups decreased effectively at 16th day as compared with the control group. 6. The activity of serum total bilirubin of the sample A, B groups decreased effectively at 16th day as compared with the control group. 7. The activity of GOT, GPT, ${\gamma}$-GTP, ALP, LDH and total bilirubin in serum of the sample C group were analogous with thats of normal group.

• Food Science and Preservation
• /
• v.10 no.1
• /
• pp.94-98
• /
• 2003

Effect of mixture of food material water extracts(modouri) composed of Job's tears, maize, buckwheat Japanese mushroom, lovage, licorice and jujube(13 : 50 : 15 : 2 : 5 : 5 : 5, v/v)] on the content of testosterone(T) and dihydrotestosterone(DHT), biochemical and histological changes of rat were investigated. Animal experiments(30 rats) were divided into 3 experimental groups(control, modouri and propecia). The summarized results were as follows: Activities of GOT, GPT, ALP, ${\gamma}$-GTP and content of total cholesterol and total lipid are normal in modouri group. Therefore modouri does not give rise to any damage in the liver. Also in the histological view, modouri does not have any hepatotoxic effect and increase the number of hair folicle. Total(T+DHT) and DHT content in rat serum and skin are significantly decrease in modouri group compare to the control but there is not any significant difference with propecia.

• Journal of Haehwa Medicine
• /
• v.13 no.2
• /
• pp.221-229
• /
• 2004

1.Purpose Stroke is the most frequent cause of mortality and morbidity rate in Korea with ischemic heart disease and cancer. Recent recurrence rate of stroke is high, but little attention had been given to the features of its' cause. The purpose of this studies is to examine the hematologic states in Korean normal adults through premature-examination of stroke(PES). 2.Method The study group consisted of 326 cases that they had no significant results by CT through premature examination of stroke(PES) in Cheonan oriental hospital of Daejeon university. We tested RBC, hemoglobin, hematocrit, WBC, PT, PTT, PLT, total protein, albumin, total bilirubin, direct bilirubin, GOT, GPT, $\gamma$-GTP, total cholesterol, triglyceride, HDL-C, glucose, BUN, creatinine, Na, K and evaluated by cross sectional study. 3.Result In the PES, there were many patients with low RBC(11%), shorten PT(26.7%), delayed PTT(19.3%), high TP, GPT, ${\gamma}$ -GTP(respective1y 16.9%, 14.4%, 13.5%), high cholesterol level and triglyceride level(respectively 18.7%, 28.2%). We didn't found significant cases in hemoglobin, hematocrit, WBC, PLT, albumin, total bilirubin, direct bilirubin, GOT, HDL-C, glucose, BUN, creatinine, Na, K. 4.Conclusion In this study, we demonstrated that short PT, high triglyceride level are special views in PES. Also prospective studies are needed continuously to search the preventing methods of the CVA.

• The Korea Journal of Herbology
• /
• v.29 no.5
• /
• pp.75-81
• /
• 2014

Objectives : This study was performed to investigate the effect of root extract of Pueraria thunbergiana Bentham (Puerariae Radix, PR) in diabetic mice as similar as emaciation-thirst disease in Oriental medicine. Methods : C57BL/6 mice were fed high fat (HF) and high sucrose (HS) for 8 weeks, and then administrated with 90 mg/kg body weight (bw) of streptozotocin (STZ) for induction of diabetes which is similar to the middle emaciation stage. After 5 days, blood glucose levels were measured, and selected the mice with ranges above $250mg/d{\ell}$. PR water extract was administrated orally once a day for 4 weeks with high fat and high sucrose. The levels of glucose, insulin, total cholesterol, triglyceride, ${\gamma}glutamyl$ transpeptidase (${\gamma}GTP$), glutamic oxaloacetic transaminase (GOT) and glutamate pyruvate transaminase (GPT) were analysed in the serum. Also, observed their histological changes by hematoxylin and eosin (H&E) of different organs, lung, heart, pancreas, stomach, liver, and kidney. Results : PR extract significantly decreased the levels of serum glucose and insulin in diabetic mice. PR extract significantly increased the levels of triglyceride, total cholesterol, GOT and GPT in diabetic mice. In H&E stain, PR extract inhibited the histopathological changes of lung (as a channel of the upper emaciation stage in the channel-tropism theory), pancreas (as a channel of the middle emaciation stage) and kidney (as a channel of the lower emaciation stage) in diabetic damage. Conclusions : PR extract has an anti-diabetic effect in HF/HS and low-dose STZ-induced diabetic mice. This result suggests that PR follows the channel-tropism theory in the emaciation-thirst disease through the protection of lung, pancreas and kidney.

• Applied Biological Chemistry
• /
• v.50 no.4
• /
• pp.295-303
• /
• 2007

A preliminary diet experiment utilizing Jerusalem artichoke's inulin, lotus leaf powder, nettle powder and eucalyptus powder extract indicated that combining all four elements gave the most effective result. Therefore, a study was conducted to evaluate the effectiveness of combined diet for weight loss. In this study, Sprague-Dawley, male white rats about 200 g in weight was fed with high fat diet for 8 weeks in order to induce obesity followed by 4 week administration of combined diet to look into the effect of the diet. After a total of 12 weeks of feeding, factors relevant to weight, blood, and lipid metabolism by liver in the body were researched and histologic change was examined with optical microscope. In terms of weight change, both high fat diet group and regular diet group gained weight from high fat diet for 8 weeks compared to normal group. Then, for another 4 weeks, while normal group and high fat diet group kept gaining weight, combined diet group which was provided with high fat diet for 8 weeks, lost weight to the normal group level after 3 week administration of diet. However, after the 4th week of administration, the group weighted significantly less than the normal group and the efficiency of diet also significantly dropped. In the biochemical analysis of blood, total cholesterol, LDL-cholesterol, triglyceride, glucose, GOT, GPT, ${\gamma}-GTP$ and creatine showed significant increase in high fat diet group and there was no significant difference between diet group and normal group except for GPT, ${\gamma}-GTP$ and creatine. In the biochemical analysis of liver, there was significant increase in LDL-cholesterol, triglyceride of high fat diet group compared to normal group, while there was no significant difference in term of total cholesterol and HDL-cholesterol. Compared to normal group, diet group had higher HDL-cholesterol, while total cholesterol dropped significantly. There was no significant difference in terms of LDL-cholesterol and triglyceride. Besides, in high fat diet group, observation of histologic change in liver and change in ultrastructure showed volume increase of hepatic cell and severe fatty degeneration in hepatic cell around hepatic vein. However in diet group, like normal group, no pathological change was observed in terms of cytoplasm, nucleus and capillary in hepatocyte and the alignment of hepatocyte had regularity thanks to the administration of combined diet. Therefore, combined diet utilizing Jerusalem artichoke's inulin, lotus leaf powder, nettle powder and eucalyptus powder was proven to be an effective measure to prevent and improve obesity as a result of abnormal adipose deposition.

• Journal of Acupuncture Research
• /
• v.22 no.3
• /
• pp.53-67
• /
• 2005

Objective : The purpose of this study is to observe the effects of Scolopendrid Aqua-acupuncture applied to the L14 on galactosamine-induced liver injury in rats. Methods : In this study, the experimental rats were divided four groups(Control group, SHA-1, SHA-2, SHA-3 group). In the Control group, we first injected galactosamine and then didn`t treated. In the SHA-1, SHA-2, SHA~3 group, we first Injected galactosamine and then injected Scolopendrid aqua-acupuncture applied to L14, each 0.083mg/kg, 0.017 mg/kg, 0.008mg/kg. We observed the changes of GOT, GPT, ${\gamma}$-GTP, Total bilirubin, LDH, ALP, Total cholesterol, Triglyceride, HDL-cholesterol, WBC, RBC, HGB, Hct. Results & Conclusion: 1. In the change of GPT content, as compared with control group, SHA-2, SHA-3 groups were significantly decreased. 2. In the change of ${\gamma}$-GTP content, as compared with control group, SHA-1, SHA-2 groups were significantly decreased. 3. In the change of Total bilirubin content, as compared with control group, SHA-2 group was significantly decreased.

• Biomedical Science Letters
• /
• v.19 no.2
• /
• pp.124-131
• /
• 2013

Genome-wide association studies (GWAS) have identified a number of common variants associated with serum liver enzyme homeostasis in population. In the previous study, single nucleotide polymorphisms (SNPs) in several genes have been reported to be associated with serum liver enzyme levels in European population. We aimed to confirm whether the genetic variation of SMAD2 (SMAD family member 2) gene influence the serum liver enzyme levels in Korean population. We genotyped variants in or near SMAD2 in a population-based sample including 994 unrelated Korean adult. Here, we performed association analysis to elucidate the possible relations of genetic polymorphisms in SMAD2 gene with serum liver enzyme levels. By examining genotype data of a total of 944 subjects in 5 hospital health promotion center, we discovered the SMAD2 gene polymorphisms are associated with serum liver enzyme levels. The common and highest significant polymorphism was rs17736760 (${\beta}$=3.51, P=5.31E-07) with glutamic oxaloacetic transferase (GOT), rs17736760 (${\beta}$=5.99, P=1.25E-05) with glutamic pyruvate transaminase (GPT), and rs17736760 (${\beta}$=15.68, P=9.93E-07) with gamma glutamyl transferase (GGT) in all group. Furthermore, the SNP rs17736760 was consistently associated with GOT (${\beta}$=5.25, P=1.72E-06), GPT (${\beta}$=9.97, P=1.16E-05), GGT (${\beta}$=26.13, P=3.43E-06) in men group. Consequently, we found statistically significant SNP in SMAD2 gene that are associated with serum levels of GOT, GPT, and GGT. In addition, these results suggest that the individuals with the minor alleles of the SNP in the SMAD2 gene may be more elevated serum liver enzyme levels in the Korean population.