• Environmental Analysis Health and Toxicology
• /
• 제1권1호
• /
• pp.61-70
• /
• 1986

The effect of polychlorinated biphenyls (PCB), phenol and biphenyl on the body, liver and kidney weights, and the activity of $\delta$-aminolevulinic acid dehydratase (ALAD), and the contents of microsomal cytochrome P-450, and hematocrit, TBAvalue. PCB (200 mg/kg), phenol (200 mg/kg), biphenyl (200 mg/kg), and biphenyl (100 mg/kg) added phenol (100 mg/kg) was treated orally to Sprague-Dawley rats for 3 days. In all treated groups, the body weights were decreased, while the weights of liver and kidney were increased in comparison with that of control group. The activity of $\delta$-ALAD was increased and hematocrit was decreased in PCB treated group, on the contrary biphenyl treated group was appeared opposite direction. The contents of microsomal cytochrome P-450 and concentration of protein were increased in all treated group. In biphenyl treated group and phenol treated group, TBA value was increased in both groups.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
• /
• pp.224-225
• /
• 2001

$\delta$-Aminolievulinic acid (ALA)는 대부분의 생물에 필수적인 tetrapyroll 화합물의 생합성 전구체로 최근 들어 다양한 의학적 용도 (피부암 치료제, 화상치료제, 면역증 강제 등)가 밝혀짐에 따라 이의 생산 및 사용범위에 대한 연구가 활발히 진행되고 있다 (Elfsson, 1998). 또한 양식어의 사육시 그 피해가 심각한 바이러스(이리도바이러스, 버나바이러스, 및 새우의 백점바이러스 등)와 원생충에 의한 감염성질병에 대하여 탁월한 예방 및 치료 효과를 나타내었으며, 저 농도의 경구투여에 의하여 항바이러스성 및 면역활성 유발 기능이 확인되어지는 등, 질병 관리를 위한 새로운 기능이 확인되었다. (중략)

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권1호
• /
• pp.97-101
• /
• 2006

Certain amino acids are essential precursors of a variety of important biomolecules in addition to their major function as protein building blocks. ${\delta}$-Aminolevulinic acid (ALA) is synthesized from the condensed form of succinyl-CoA with glycine after decarboxylation catalyzed by ALA synthase. The objective of the study was to determine the effects of ALA supplementation on growth performance, behavioral characteristics and hematological/immune status in nursery pigs. A total of 144 pigs weaned at 21 d of age were allotted to three dietary treatments representing (-) control (w/o antibiotics; NC), (+) control (w/carbadox at 50 mg/kg; PC), and the treatment group with ALA supplementation (0.05%; TA). Each treatment had 6 pens (replicates) with 8 pigs per pen. Pigs were fed phase 1 (21.9% CP, 1.40% Lys) and 2 (20.6% CP, 1.15% Lys) experimental diets for 3 and 2 wks, respectively. Feed intake and weight gain were measured weekly during phase 1 and at the end of phase 2. At the end of phase 2, blood samples were taken and analyzed using an automated hematology analyzer. Skin color and activity of pigs (48 h) from all pens in each treatment were measured at the second week of phase 2. Growth performance was not affected (p>0.05) by the dietary supplementation of ALA during the 5 wk nursery period. Pigs in the TA (6.46) and PC (6.68) had a higher (p<0.05) number of red blood cells ($10^6cell/{\mu}L$) than pigs in the NC (6.15). Pigs in PC (12.16) had a higher (p<0.05) hemoglobin level (g/dL) than pigs in the NC group (11.29) and the TA group (11.47). Pigs in the TA and PC had darker (p<0.05) and less (p<0.05) yellow skin color than pigs in the NC. Pigs in the PC tended (p = 0.081) to be less active than pigs in the other groups. There were no differences in behavioral characteristics between the NC and the TA. The data suggest that ALA supplementation has no adverse effects on growth performance of nursery pigs. Moreover, ALA supplementation increased red blood cell counts which may be beneficial to pigs.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 춘계학술발표대회
• /
• pp.512-515
• /
• 2000

일정전류와 일정전압조건 모두에서 완충액 농도가 증가할수록 시료들의 이동시간이 증가하였으며 전압이 높을수록 시료들의 이동시간이 단축됨을 알 수 있었다. ALA, PBG, glycine, LA의 최적 분석조건은 borate 완충액 30mM, 20kV, $76{\mu}A$에서 가장 잘 분리 되었다. 본 연구를 통하여 ALA, PBG, glycine, LA를 동시에 분리할 수 있을 뿐만 아니라 ALA 2mM, PBG 0.25mM 범위내에서 선형적인 보정곡선을 보이므로 정량분석이 가능하였다. CE를 이용한 ALA와 PBG의 동시 정량분석으로 인체, 동식물 및 광합성 미생물의 대사과정에 수반되는 효소인 ALA synthetase, ALA dehydratase의 활성 연구, 광합성 홍색세균에 의한 ALA생합성, 의학, 생화학, 효소공학 등에 응용할 수 있을 것으로 기대된다.

• 한국산업보건학회지
• /
• 제23권2호
• /
• pp.65-74
• /
• 2013

Objectives: This study attempted to develop a method to measure ultra-trace lead concentrations in plasma using Inductively Coupled Plasma Mass Spectrometry(ICP-MS) and to test whether plasma lead can be used as a biomarker for the biological monitoring of exposure to lead. Methods: Lead concentrations in 160 plasma samples of field workers and 42 plasma samples from the control group were measured by ICP-MS. Blood zinc protophorphyrin(ZPP) concentrations and urinary ${\delta}$-aminolevulinic acid${\delta}-ALA$) were measured for correlation analysis with plasma lead. Results: The mean lead level in the plasma of the workers exposed to lead at work were 786.1 ng/L. Plasma lead levels were not correlated with blood ZPP or urinary ${\delta}-ALA$ concentrations. Otherwise, plasma lead levels showed a good correlation coefficient of 0.400 with blood lead levels, and their correlation coefficient had a better value of 0.552 for the non-smoking and drinking group. In the general population group which was not exposed to lead in the workplace and was considered the control group, the mean concentration of plasma lead was 123.1 ng/L. The plasma lead levels for the general population group showed a good correlation coefficient of 0.520 with blood ZPP and urinary ${\delta}-ALA$ concentrations.

• 한국식품영양과학회지
• /
• 제20권4호
• /
• pp.337-345
• /
• 1991

식이 단백질과 칼슘의 수준이 납중독된 흰쥐의 혈액학적 성상 및 신기능에 미치는 영향을 관찰하기 위해, 흰쥐에게 납 2,000ppm을 함유한 식수를 제한 없이 주면서, 식이 단백질과 칼슘 수준을 각각 3수준, 사육기간을 3주와 7주로 한, 3$\times$3$\times$2 요인 실험으로 설계하고 대조군을 별도로 설정하여 조사한 결과는 다음과 같다. 납 급여군의 체중 증가량, 식이 섭취량 및 식이 효율은 대조군에 비해 감소하였고, 단백급여 수준이 정상보다 높으면서 칼슘 급여 수준이 높을 때 높게 나타났다. 간장, 신장, 비장 및 뇌의 체중 100g당 무게는 대조군에 비해 증가하였으며, 식이 칼슘 수준이 낮을 수록 증가했다. 헤마토크릿치, 헤모글로빈 함량 및 적혈구 수는 납 급여군에서 감소를 보였고, 식이 단백질과 칼슘 수준이 낮을 수록 감소하는 경향이었다. 뇨중 $\delta$-aminolevulinic acid 배설량은 납 급여시 증가하였고, 식이 칼슘 함량이 낮을수록 증가하였다. 뇨중 포도당 배설량은 납 급여 시 증가하였고. 식이 단백과 칼슘 함량이 낮을 수록 높아지는 경향을 보였다. Creatinine clearance는 식이 단백과 칼슘 함량이 충분할 때는 납의 영향을 받지 않는 것으로 나타났다.

• 환경위생공학
• /
• 제14권3호
• /
• pp.107-115
• /
• 1999

The effectiveness of resting cells of a photosynthetic bacterium, Rhodospirillum rubrum N-1, was investigated on the production of extracellular ${\delta}-aminolevulinic$ acid(ALA). The ALA generating system required 3hr-incubation in the presence of 10mg of resting cells per ml to obtain the maximal yield of extracellular ALA. and also, under this condition the effect of ALA inducers, i.e., 30mM levulinic acid (LA) and L-glutamic acid($C_5$ pathway precursor) was relatively higher than that of produced extracellular ALA($83{\mu}M$). The volume of system and proper cell density appeared to be important factors for the effective production of extracellular ALA.

• 생약학회지
• /
• 제29권4호
• /
• pp.374-378
• /
• 1998

Sodium arsenate and Paljin-Tang extract (PJT), a herbal restorative were treated p.o. 20 mg/kg and 500 mg/kg, respectively, and concurrently to rats, and examined the effects on the liver of rats. The values of protein, aniline hydroxylase (AH) and 2-thiobarbituric acid (TBA) were increased in arsenic-treated group. The values of glucose-6-phosphatase (G-6-P) and ${\delta}-aminolevulinic$ acid dehydratase (ALAD) of arsenic-treated group were decreased. But concurrent ad-ministration with PJT showed significant recovery from the toxicity of arsenic.

• 약학회지
• /
• 제44권6호
• /
• pp.552-557
• /
• 2000

In order to investigate the effects of Yukmijihwang-Tang on the hepatic microsomal function of Cd-poisoned rats, 3 mg/kg of cadmium (Cd) and 500 mg/kg of Yukmijihwang-Tang extract (YJT), a herbal hepatoprotective medicine, were administered concurrently to rats for 4 weeks. The levels of protein, aniline hydroxylase (AH) and malondialdehyde (MDA) were increased in Cd-treated group. This increase was suppressed by treatment or YJT. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glucose-6-phosphatase (G-6-P) and ${\delta}-aminolevulinic$ acid dehydratase (ALAD) of Cd-treated group were decreased. This decrease was inhibited by treatment of YJT. Treatment with YJT significantly protects cadmium-induced hepatotoxicity.

• 대한수의학회지
• /
• 제24권1호
• /
• pp.24-30
• /
• 1984

The protective effects of high levels of dietary iron, copper, zinc or protein on lead toxicity were studied In chicks. Growth retardation, reduction of feed intake, anemia and accumulation of lead in the bone and kidney were observed in chicks fed a diet containing 500mg lead as chloride per kg of feed for 42 days. Early changes due to ingested lead were inhibition of red blood cell ${\delta}$-aminolevulinic acid dehydrase at all doses and no effect of iron, copper, zinc or protein addition were observed. Tibia lead accumulation was reduced in chicks receiving additional dietary iron or zinc compared to the lead only group but increased in chicks given supplementary protein. Decreased body weight gain was overcome by supplementary zinc or protein in chicks fed lead but not by supplementary iron. Overall the results of this study show that lead poisoning can be partly reduced by providing supplementary iron, zinc or protein, but the interaction of these element remained to be elucidated.