• 생명과학회지
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• 제11권4호
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• pp.385-391
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• 2001

Filamentous hemagglutinin (FHA) is considered as an essential immunogenic component for incorporation into acellular vaccines against Bordetella pertussis, the causative agent of whooping cough. Classically, antipertussis vaccination has employed an intramuscular route. An alternative approach to stimulate mucosal and systemic immune responses is oral immunization with recombinant live vaccine carrier strains of Salmonella typhimurium. An attenuated live Salmonella vaccine sgrain($\Delta$cya $\Delta$crp) expressing recombinant FHA(rFHA) was developed. Stable expressionof rFHA was achieved by the use of balanced-lethal vector-host system. which employs an asd deletion in the host chromosome to impose in obligate requirement for diaminopimelic acid. The chromosomal $\Delta$asd mutation was complemented by a plasmid vector possessing the asd$^{+}$ gene. A 3 kb DNA fragment encoding immuno dominant regionof FHA was subcloned in-frame downstream to the ATG translation initiation codon in the multicopy Asd$^{+}$ pYA3341 vector to create pYA3457. Salmonella vaccine harboring pYA3457 expressed approximately 105kDa rFHA protein. The 100% maintenance of [YA3457 in vaccine strain was confirmed by stability examinations. Additionally, a recombinant plasmid pYA3458 was constructed to overpress His(8X)-tagged rFHA in Essherichia coli. His-tagged rFHA was purified from the E. coli strain harboring pYA3458 using Ni$^{2+}$-NTA affinity purification system.>$^{2+}$-NTA affinity purification system.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2003년도 춘계학술대회 논문집 유기절연재료 방전 플라즈마연구회
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• pp.53-56
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• 2003

In this paper we investigated electrical properties for epoxy resin with improved mechanical property, elastomer epoxy. Investigated electrical properties are permittivity, tan $\delta$ and breakdown voltage strength(BDV). Permittivity and tan $\delta$ have dependancy on additive Quantity in general purpose epoxy resin. In particularly, those have very high values at low frequency and high temperature according to increasing component of elastomer. In case of BDV test, those materials have only a little difference due to increasing elastomer components. But in case of high quantities of elastomer, BDV has a difference. These results are represented that elastomer epoxy resin despite superior mechanical property needs many carefully thought as application electrical insulation.

• Journal of Microbiology and Biotechnology
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• 제24권9호
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• pp.1207-1215
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• 2014

Candida albicans, the major human fungal pathogen, undergoes morphological transition from the budding yeast form to filamentous growth in response to nitrogen starvation. In this study, we identified a new function of GST2, whose expression was required for filamentous growth of C. albicans under nitrogen-limiting conditions. The Gst2p showed Gst activity and required response to oxidative stress. The ${\Delta}gst2$ mutant displayed predominantly yeast phase growth in low ammonium media. Such morphological defect of ${\Delta}gst2$ mutants was not rescued by overexpression of Mep2p, Cph1p, or Efg1p, but was rescued by either overexpression of a hyperactive $RAS1^{G13V}$ allele or through exogenous addition of cyclic AMP. In addition, the ${\Delta}gst2$ mutants had lower levels of RAS1 transcripts than wild-type cells under conditions of nitrogen starvation. These results were consistent with the Ras1-cAMP pathway as a possible downstream target of Gst2p. These findings suggest that Gst2p is a significant component of nitrogen starvation-induced filamentation in C. albicans.

• Kyungpook Mathematical Journal
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• 제47권3호
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• pp.335-340
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• 2007

Let M be a hyperbolic 3-manifold such that ${\partial}M$ has at least two boundary tori ${\partial}_oM$ and ${\partial}_1M$. Suppose that M contains an essential orientable surface P of genus $g$ with one outer boundary component ${\partial}_oP$, lying in ${\partial}_oM$ and having slope ${\lambda}$ in ${\partial}_oM$, and $p$ inner boundary components ${\partial}_iP$, $i=1$, ${\cdots}$, $p$, each having slope ${\alpha}$ in ${\partial}_1M$. Let ${\beta}$ be a slope in ${\partial}_1M$ and suppose that $M({\beta})$ is toroidal. Let $\hat{T}$ be a minimal essential torus in $M({\beta})$, which means that $\hat{T}$ is pierced a minimal number of times by the core of the ${\beta}$-Dehn filling, among all essential tori in $M({\beta})$. Let $T=\hat{T}{\cap}M$ and denote by $t$ the number of components of ${\partial}T$. In this paper we prove: (i) if $t{\geq}3$, then ${\Delta}({\alpha},{\beta}){\leq}6+\frac{10g-5}{p}$, (ii) If $t=2$, then ${\Delta}({\alpha},{\beta}){\leq}13+\frac{24g-12}{p}$, (iii) If $t=1$, then ${\Delta}({\alpha},{\beta}){\leq}1$.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2003년도 추계학술대회 논문집 Vol.16
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• pp.67-70
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• 2003

The control of tilt angle for nematic liquid crystal (NLC) with negative and positive dielectric anisotropy on the rubbed homeotropic polyimide (PI) using baking method by hot plate equipment was investigated. LC tilt angle decreased with increasing baking temperature and time. Especially, the low LC tilt angle of positive type NLC ($\Delta\varepsilon$ >0) on the rubbed homeotropic PI surface by increasing temperature and time was measured. The tilt angle of positive type NLC ($\Delta\varepsilon$ >0) is smaller than that of the negative type ($\Delta\varepsilon$ >0) on rubbed PI with increasing baking temperature and time. We consider that the tilt angle of NLC is decreased due to increasing the steric interaction between horizon component of permittivity $\varepsilon$ = of NLC and the stress of polymer side chain by high temperature.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2022년도 학술발표회
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• pp.85-85
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• 2022

The world's most extensive and active deltas, Louisiana's wetlands, are deteriorating rapidly due to multiple stressors such as the discharge of the Mississippi River, sea-level rise, and coastal retreat, the substantial but spatially and temporally variable impacts. However, the ecological and anthropogenic histories, the mode of environmental changes on a multi-millennial timescale have not been thoroughly documented. This study, a palynology-based multiproxy analysis, investigates hydrological, geological, geochemical, and anthropogenic impacts on southern Louisiana wetlands and a variety of external forcing agents influencing ecological succession. Sediment cores extracted from a small pond on a mangrove-dominate island near Port Fourchon, Louisiana, USA yielded a 4,000-year record. The site has been transformed from freshwater to saline water environments, to a mangrove dominant island over the late Holocene. The multivariate principal component analysis identified the relative strength of external drivers responsible for each ecological shift. The Mississippi River delta cycle (lobe switching) was the dominant driver of ecosystem changes during the late Holocene, while relative sea-level rise, tropical cyclones, climate, and anthropogenic effects have been the main drivers late in the site's history.

• 한국전기전자재료학회논문지
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• 제16권6호
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• pp.538-548
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• 2003

We studied effects by Re$_2$O$_3$(R=Dy, Gd, Ho) addition on the properties of Mn-Zn ferrite. The doping concentration range from 0.05 wt% to 0.25 wt%. All samples were prepared by standard fabrication of ceramics. With increasing the rare earth oxides, specific density and initial permeability increased on the whole. But, the tendencies such as upper result had the measured value on limitation and characteristics saturated or decreased properties after that. In case of excessive addition of additive beyond some level, initial permeability properties of ferrite have gone down in spite of anomalous grain. With increasing the content of additive, both the real and imaginary component of complex permeability and the magnetic loss (tan$\delta$) increased. Because the increased rate of real component had higher than imaginary component, magnetic loss increased none the less for increasing the real component related with magnetic permeability. But, the magnetic loss of ferrite doped with the rare earth oxides was lower than that of Mn-Zn ferrite at any rate. The small amount of present rare earth oxides in Mn-Zn ferrite composition led to enhancement of resistivity in bulk, and more so in the grain boundary. It was seem to be due to the formation of mutual reaction such as between iron ions and rare earth element ions.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1999년도 하계학술대회 논문집 D
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• pp.1815-1817
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• 1999

In this paper, the generation of high pretilt angle in nematic liquid crystal (NLC) by dipping effect on three kinds of the rubbed polyimide (PI) surface was investigated. The generated pretilt angle of NLC increases due to dipping effect before rubbing treatment on two kinds of the rubbed PI surface with side chain. The generated pretit angle in NLC by using dipping effect after rubbing treatment on PI surface with short side chain is high compared to the PI surface with long side chain. The generated pretit angle of the positive type NLC $(\Delta{\varepsilon}>0)$ is higher as than that of the negative type NLC $({\Delta}{\varepsilon}<0)$ by using dipping effect before rubbing treatment on homeotropic layer. The generated pretilt angle of NLC is attributed to perpendicular component of permittivity ${\varepsilon}_{\perp}$ of NLC on rubbed PI surface.

• 한국의류학회지
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• 제37권4호
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• pp.506-513
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• 2013

Rubia cordifolia L (Indian madder) contains red color purpurin (65-67%) and yellow color munjistin (10-12%) as well as nordamncanthal (9-10%). Purpurin is a traditional red dye. The purpose of this research is to increase the dyeability of silk and light fastness of dyed silk fabric. We dyed silk fabrics after pretreatment to remove the yellow component of madder using various solvents such as ethyl acetate, ethanol, methanol, chloroform, and acetone. The total K/S value was the highest in the case of chloroform and reflectance was the lowest at the red color region from 470nm to 530nm. Chloroform dissolved the yellow color of Rubia cordifolia; in addition, we found that the total K/S value increased and the ${\Delta}E$ value decreased by chloroform pretreatment for silk dyeing.

• 한국산학기술학회논문지
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• 제17권10호
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• pp.319-330
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• 2016

3D 프린팅 기술은 다양한 산업분야에서 개념모델 및 기능성 시제품을 제작하는데 많이 응용되고 있지만, 3D 프린팅 소재 및 제작된 제품 신뢰성 등의 여러 가지 이유로 상용화 제품으로 적용되는 데 한계가 존재한다. 본 논문에서는 3D 프린팅 기술을 이용한 산업적 응용분야 중 하나로 발전소 저탄장에 사용되는 풀 코드 스위치 모듈의 부품들 중 잦은 돌발 상황으로 인해 파손이 자주 발생하는 허브 구동부와 레버 고정부 부품들에 대해 현장에서 작업자들이 단기 대체부품으로 적용이 가능하도록 하는 부품 최적 설계 및 FDM 방식 3D 프린팅 제조공정기술에 대한 연구결과를 제시하였다. 3D 프린팅 기술의 경우 소재 적용에 있어서 한계가 존재하므로, 본 논문에서는 구조 최적화 설계를 통해 구조 안정성을 확보하는 방안에 대해 연구를 수행하였다. 허브 구동부 부품에 대해 내부 구조 형상 및 구조 설계 변수 최적화를 수행하여 좌측구동모드에서는 안전계수가 153.67% 증가한 1.243을 확보할 수 있었으며, 우측구동모드에서는 404.96% 증가한 3.156을 확보할 수 있었다. 레버 고정부 부품의 경우, 반복적인 스위칭 구동에 의한 굽힘 모멘트로 인해 발생하는 파손을 최소화하기 위해 구조 최적화 설계를 수행하여 26% 증가한 구조 안전계수(7.52)를 확보할 수 있었다. 본 연구를 통해 3D 프린팅 기술을 단기 대체부품 제조공정에 적용함에 있어서 소재 최적화를 통한 설계보다는 3D 프린팅 공정의 적층특성을 활용한 구조적 최적화 설계기법 이 더욱 유연한 결과를 도출할 수 있음을 확인할 수 있었다.